http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Hyun Ju Lee ),( Ja Sung Rho ),( Shao Ran Gui ),( Mi Kyung Kim ),( Yu Kyoung Lee ),( Yeon Sook Lee ),( Jeong Eun Kim ),( Eu Na Cho ),( Mong Cho ),( Tae Ho Hwang ) 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3
Background/Aims: JX-594 is an oncolytic virus derived from the Wyeth vaccinia strain that causes replication-dependent cytolysis and antitumor immunity. Starting with a cross-examination of clinical-trial samples from advanced hepatocellular carcinoma patients having high levels of aldosterone and virus amplification in JX-594 treatment, we investigated the association between virus amplification and aldosterone in human cancer cell lines. Methods: Cell proliferation was determined by a cell-counting-kit-based colorimetric assay, and vaccinia virus quantitation was performed by quantitative polymerase chain reaction (qPCR) and a viral plaque assay. Also, the intracellular pH was measured using a pH-sensitive dye. Results: Simultaneous treatment with JX-594 and aldosterone significantly increased viral replication in A2780, PC-3, and HepG2 cell lines, but not in U2OS cell lines. Furthermore, the aldosterone treatment time altered the JX-594 replication according to the cell line. The JX-594 replication peaked after 48 and 24 hours of treatment in PC-3 and HepG2 cells, respectively. qPCR showed that JX-594 entry across the plasma membrane was increased, however, the changes are not significant by the treatment. This was inhibited by treatment with spironolactone (an aldosterone-receptor inhibitor). JX-594 entry was significantly decreased by treatment with EIPA [5-(N-ethyl-N-isopropyl)amiloride; a Na+/H+-exchange inhibitor], but aldosterone significantly restored JX-594 entry even in the presence of EIPA. Intracellular alkalization was observed after aldosterone treatment but was acidified by EIPA treatment. Conclusions: Aldosterone stimulates JX-594 amplification via increased virus entry by affecting the H+ gradient. (Korean J Hepatol 2011;17:213-219)
영어 대화연속체에서 한국대학생들의 부사어구 사용에 대한 연구
조은하 ( Eu Na Cho ) 21세기영어영문학회 2014 영어영문학21 Vol.27 No.2
This research explores the Korean university students`` use of English adverbials by studying how adverbials contribute to pragmatic tone. English oral data from 80 participants whose native language is Korean were transcribed and analysed. All adverbials that have assertive tone and intense tone were identified and grouped by proficiency level of the Korean university students. The results show that both the range and frequency of adverbials used are very similar between the lower- and higher-proficiency groups. Both groups use adverb “so” that has intense tone far more frequently than other adverbials. However, the two groups differ in their use of adverbials to convey pragmatic tone. The lower-proficiency learners tend to rely more upon intensifiers by repeatedly using a limited number of adverbs. Compared with the lower-proficiency group, the higher-proficiency learners have a tendency to use various adverbials in interactional contexts. The findings indicate that pragmatic proficiency should be studied in the skillful use of adverbials to convey messages. Finally, EFL teachers should consider that the appropriate use of adverbs which are pragmatically rich items contributes to the learners`` utterance.
상백피 및 강황 추출 혼합물 첨가에 따른 생면의 저장성 및 품질증진 효과
박나비(Na-Bi Park),이소영(So-Young Lee),윤소영(So-Young Yoon),김꽃봉우리(Koth-Bong-Woo-Ri Kim),송유진(Eu-Jin Song),이소정(So-Jeong Lee),이청조(Chung-Jo Lee),정지연(Ji-Yeon Jung),곽지희(Ji-Hee Kwak),이호동(Ho-Dong Lee),최호덕(Ho-Duk Cho 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.5
This study was performed to examine the shelf life and qualities of wet noodle with Morus alba L. root and Curcuma aromatica extracts (MCE) during storage at 4oC. Lightness and redness of wet noodle were decreased with increasing amounts of MCE added in noodle while yellowness was increased. The viable cell and molds count of wet noodle with MCE was reduced about 1~2 ? cycle as compared with control during storage time. Also the TBARS (thiobarbituric acid reactive substance) value of wet noodle with MCE was lower than that of control. Hence the wet noodle with MCE has shown remarkable antioxidation effect. In sensory evaluation, the wet noodle containing the ratio of 2.5:0.02 of M. alba : C. aromatica was preferred than the control. From these results, the addition of 2.5% of M. alba and 0.02% of C. aromatica extracts in wet noodle had a good effect on improvement of preservation and development of quality.
MicroRNA-23a: A Novel Serum Based Diagnostic Biomarker for Lung Adenocarcinoma
Lee, Yu-Mi,Cho, Hyun-Jung,Lee, Soo-Young,Yun, Seong-Cheol,Kim, Ji-Hye,Lee, Shin-Yup,Kwon, Sun-Jung,Choi, Eu-Gene,Na, Moon-Jun,Kang, Jae-Ku,Son, Ji-Woong The Korean Academy of Tuberculosis and Respiratory 2011 Tuberculosis and Respiratory Diseases Vol.71 No.1
Background: MicroRNAs (miRNAs) have demonstrated their potential as biomarkers for lung cancer diagnosis. In recent years, miRNAs have been found in body fluids such as serum, plasma, urine and saliva. Circulating miRNAs are highly stable and resistant to RNase activity along with, extreme pH and temperatures in serum and plasma. In this study, we investigated serum miRNA profiles that can be used as a diagnostic biomarker of non-small cell lung cancer (NSCLC). Methods: We compared the expression profile of miRNAs in the plasma of patients diagnosed with lung cancer using an miRNA microarray. The data from this assay were validated by quantitative real-time PCR (qRT-PCR). Results: Six miRNAs were overexpressed and three miRNAs were underexpressed in both tissue and serum from squamous cell carcinoma (SCC) patients. Sixteen miRNAs were overexpressed and twenty two miRNAs were underexpressed in both tissue and serum from adenocarcinoma (AC) patients. Of the four miRNAs chosen for qRT-PCR analysis, the expression of miR-23a was consistent with microarray results from AC patients. Receiver operating characteristic (ROC) curve analyses were done and revealed that the level of serum miR-23a was a potential marker for discriminating AC patients from chronic obstructive pulmonary disease (COPD) patients. Conclusion: Although a small number of patients were examined, the results from our study suggest that serum miR-23a can be used in the diagnosis of AC.
MicroRNA-23a: A Novel Serum Based Diagnostic Biomarker for Lung Adenocarcinoma
( Yu Mi Lee ),( Hyun Jung Cho ),( Soo Young Lee ),( Seong Cheol Yun ),( Ji Hye Kim ),( Shin Yup Lee ),( Sun Jung Kwon ),( Eu Gene Choi ),( Moon Jun Na ),( Jae Ku Kang ),( Ji Woong Son ) 대한결핵 및 호흡기학회 2011 Tuberculosis and Respiratory Diseases Vol.71 No.1
Background: MicroRNAs (miRNAs) have demonstrated their potential as biomarkers for lung cancer diagnosis. In recent years, miRNAs have been found in body fluids such as serum, plasma, urine and saliva. Circulating miRNAs are highly stable and resistant to RNase activity along with, extreme pH and temperatures in serum and plasma. In this study, we investigated serum miRNA profiles that can be used as a diagnostic biomarker of non-small cell lung cancer (NSCLC). Methods: We compared the expression profile of miRNAs in the plasma of patients diagnosed with lung cancer using an miRNA microarray. The data from this assay were validated by quantitative real-time PCR (qRT-PCR). Results: Six miRNAs were overexpressed and three miRNAs were underexpressed in both tissue and serum from squamous cell carcinoma (SCC) patients. Sixteen miRNAs were overexpressed and twenty two miRNAs were underexpressed in both tissue and serum from adenocarcinoma (AC) patients. Of the four miRNAs chosen for qRT-PCR analysis, the expression of miR-23a was consistent with microarray results from AC patients. Receiver operating characteristic (ROC) curve analyses were done and revealed that the level of serum miR-23a was a potential marker for discriminating AC patients from chronic obstructive pulmonary disease (COPD) patients. Conclusion: Although a small number of patients were examined, the results from our study suggest that serum miR-23a can be used in the diagnosis of AC.
가시오가피와 두충 추출 혼합물 첨가에 의한 생면의 저장성 및 품질 증진 효과
정지연(Ji-Yeon Jung),송유진(Eu-Jin Song),이소영(So-Young Lee),김꽃봉우리(Koth-Bong-Woo-Ri Kim),이소정(So-Jeong Lee),윤소영(So-Young Yoon),이청조(Chung-Jo Lee),박나비(Na-Bi Park),곽지희(Ji-Hee Kwak),이호동(Ho-Dong Lee),최호덕(Ho-Duk Cho 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.6
1.5% 농도의 가시오가피와 두충 추출 혼합물 첨가가 생면의 저장성과 품질에 미치는 영향에 대하여 알아보았다. 가시오가피와 두충 추출 혼합물을 첨가한 생면을 4℃에서 21일간 저장하며 일반 세균수와 곰팡이수를 측정한 결과, 저장기간 전반에 걸쳐서 처리구가 무처리구보다 미생물의 생육이 억제되었다. 산화도의 경우 처리구가 무처리구보다 낮은 값을 보여 추출물에 의해 생면의 산화가 지연됨을 알 수 있었다. pH의 변화에 있어서는 저장기간 동안 일정하게 유지되었으며, 실험구간의 pH는 유의적인 차이를 나타내지 않았다. 수분함량은 9일까지 유의적인 차이를 보이지 않았으나 15일과 21일에는 처리구가 무처리구보다 다소 높은 수분함량을 보였다. 색도의 경우에는 처리구의 명도와 황색도가 무처리구보다 다소 낮은 값을 나타냈다. 물성에서는 저장기간 전반에 걸쳐 처리구와 무처리구 간의 유의적인 차이를 보이지 않았다. 관능평가에서 맛은 처리구가 무처리구보다 낮은 기호도를 보였지만, 전체적인 기호도에서는 처리구와 무처리구가 유사한 기호도를 나타냈다. 이상의 결과를 종합해 볼 때 가시오가피와 두충 추출 혼합물을 1.5% 첨가할 경우 생면의 품질을 유지하면서 저장성 개선에 효과가 있을 것으로 사료된다. This study examined the shelf-life and quality characteristics of the wet noodle added with the Acanthopanax senticosus and Eucommia ulmoides extract (AEE). The wet noodle with AEE reduced total microbial counts and TBARS value compared with the control during storage at 4℃ for 21 days. Moisture content of the wet noodle with AEE and control was not different during the early storage period, but after 9 days of storage, that of the control was decreased about 7%, while that of the noodle with AEE was decreased slightly. Lightness of the wet noodle with AEE showed low value in uncooked and cooked noodles compared with the control and decreased during storage. The pH and texture of the wet noodle with AEE and control did not change during storage at 4℃ for 21 days. In sensory evaluation, texture, soup smell and springiness of the wet noodle with AEE were preferred than the control. These results suggested that the AEE can keep the quality characteristics and increase the shelf-life in the wet noodle.