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Pandey Bishnu Prasad,Pradhan Suman Prakash 경희대학교 융합한의과학연구소 2022 Oriental Pharmacy and Experimental Medicine Vol.22 No.1
The aim of this study was to examine antioxidant properties, major enzyme inhibition activities, and targeted metabolites profiling of Lepisorus mehrae, Pleurospermum benthamii, and Roscoea auriculata in different solvent extracts. This is the first report on metabolites profiling and biochemical activities of these plant species. Our results revealed that L. mehrae, P. benthamii, and R. auriculata are rich source of bioactive secondary metabolites and have good antioxidant potential. The methanol extract of L. mehrae, P. benthamii, and R. auriculata showed substantial inhibitory potential towards elastase, whereas water extract of L. mehrae and R. auriculata were more strong inhibitors of tyrosinase. Among the three plants, P. benthamii showed noteworthy inhibition on α-amylase, α-glucosidase, lipase, tyrosinase, elastase, and cholinesterases enzymes. High resolution mass spectrometry analysis revealed the presence of metabolites such as Protocatechuic acid, Gal- lic acid, 7,8,3′,4′-tetrahydroxyflavanone, Rhamnocitrin, Quercetin, Hyperoside, Quercetin-7-glucoside, Rutin, Rhamnetin, Aromadendrin, Camphor, and Hexanoic acid in L. mehrae. Moreover, Catechin and Fisetin were present in P. benthamii and Kaempferide, 7,8,3′,4′-Tetrahydroxyflavone, 5,7-Dihydroxy-2,3-dihydroflavonol 3-acetate, and Aromadendrin were present in R. auriculata. The results presented here provide enough scientific evidence that these plant species have diverse biochemi- cal potential and can be examined further for their potential use in modern pharmaceuticals, cosmetics, and nutraceuticals.
Regioselective hydroxylation of daidzein using P450 (CYP105D7) from Streptomyces avermitilis MA4680
Pandey, Bishnu Prasad,Roh, Changhyun,Choi, Kwon-Young,Lee, Nahum,Kim, Eun Jung,Ko, Sungghi,Kim, Taejin,Yun, Hyundon,Kim, Byung-Gee Wiley Subscription Services, Inc., A Wiley Company 2010 Biotechnology and bioengineering Vol.105 No.4
<P>Regiospecific 3′-hydroxylation reaction of daidzein was performed with CYP105D7 from Streptomyces avermitilis MA4680 expressed in Escherichia coli. The apparent K<SUB>m</SUB> and k<SUB>cat</SUB> values of CYP105D7 for daidzein were 21.83 ± 6.3 µM and 15.01 ± 0.6 min<SUP>−1</SUP> in the presence of 1 µM of CYP105D7, putidaredoxin (CamB) and putidaredoxin reductase (CamA), respectively. When CYP105D7 was expressed in S. avermitilis MA4680, its cytochrome P450 activity was confirmed by the CO-difference spectra at 450 nm using the whole cell extract. When the whole-cell reaction for the 3′-hydroxylation reaction of daidzein was carried out with 100 µM of daidzein in 100 mM of phosphate buffer (pH 7.5), the recombinant S. avermitilis grown in R2YE media overexpressing CYP105D7 and ferredoxin FdxH (SAV7470) showed a 3.6-fold higher conversion yield (24%) than the corresponding wild type cell (6.7%). In a 7 L (working volume 3 L) jar fermentor, the recombinants S. avermitilis grown in R2YE media produced 112.5 mg of 7,3′,4′-trihydroxyisoflavone (i.e., 29.5% conversion yield) from 381 mg of daidzein in 15 h. Biotechnol. Bioeng. 2010. 105: 697–704. © 2009 Wiley Periodicals.</P>
Pandey, Bishnu Prasad,Lee, Nahum,Choi, Kwon-Young,Jung, Eunok,Jeong, Da-hye,Kim, Byung-Gee Elsevier 2011 Enzyme and microbial technology Vol.48 No.4
<P><B>Abstract</B></P><P>Screening of cytochrome P450 monoxygenases responsible for the regiospecific hydroxylation of flavones, isoflavones and chalcones was attempted using a P450 library constructed from <I>Streptomyces avermitilis</I> MA4680, <I>Bacillus</I> and <I>Nocardia farcinica</I> IFM10152 strains. As electron transfer redox partners with the P450s in <I>Escherichia coli</I> system, putidaredoxin reductase (PdR) and putidaredoxin (Pdx) from <I>Pseudomonas putida</I> were used. Among the 50 soluble P450s in the library screened, three cytochrome P450s, i.e. CYP107Y1, CYP125A2 and CYP107P2 from <I>S. avermitilis</I> MA4680 showed good hydroxylation activities towards flavones and isoflavones. However, low product yields prevented us from identifying complete structure of the products. By using <I>S. avermitilis</I> MA4680 as their expression host, further analysis identified that CYP107Y1(SAV2377), CYP125A2(SAV5841) and CYP107P2(SAV4539) showed good regiospecific hydroxylation activities towards genistein (4′,5,7-trihydroxyisoflavone), chrysin (5,7-dihydroxyisoflavone) and apigenin (4′,5,7-dihydroxyisoflavone) to produce 3′,4′,5,7,-tetrahydroxyisoflavone, B-ring hydroxylated 5,7-dihydroxyflavone and 3′,4′,5,7,-tetrahydroxyflavone, respectively. Analyses of the reaction products were performed using HPLC, ESI-MS–MS and GC–MS and 1H NMR.</P>
Pandey Bishnu Prasad,Pradhan Suman Prakash,Adhikari Kapil,Shresth Rajib Kumar 한국미생물학회 2021 미생물학회지 Vol.57 No.3
Streptomyces are widely distributed in soil and known for the production of bioactive secondary metabolites. It has been reported that among microbial-derived antibiotics, two-third are produced by the Streptomyces species alone. Hence, continuous screening of the Streptomyces species is of growing scientific interest. A small Himalayan country like Nepal is in a unique geographical location with a huge biodiversity. However, little is known about microbial diversity. The aim of this study was to isolate and characterize the Streptomyces species from a high-altitude soil sample collected from an altitude of 4,380 meters above sea level. The 16S rRNA sequence analysis revealed that four isolated strains; G-10, G-14, G-18, and S4L belong to the Streptomyces species. On the basis of phylogenetic analysis of 16S rRNA gene sequences of the isolates with the best match in the database revealed that G-18 isolates closely related to Streptomyces albidoflavus strain PAS-12. Moreover, the ranges of radical scavenging activities by crude extract of isolates were observed against DPPH and ABTS. Furthermore, crude extract of isolates revealed the range of antimicrobial activities against the four pathogenic strains namely Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus species, and Bacillus substilis. Moreover, isolated Streptomyces species revealed amylase, cellulase, and L-asparaginase enzyme activities.
( Kumud Raj Kafle ),( Bishnu Prasad Pandey ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2
Nepal, constantly ranked as one of the most disaster-prone countries, has a high risk of landslides. This study aims at creating the hazard map, landslide inventory map and designation of hazard levels along ~100Kms of Sub-Himalayan road in four of the most crucial sites. Unmanned Aerial Vehicles (UAVs) as the primary means of for carrying out the topographical survey, used the Digital Surface Model (DSM) and the orthomosaic map. The Root Mean Square Error (RMSE) of the survey for the individual sites were found to be 0.001m, 0.045 m, 0.044 m and 0.804 m respectively. A detailed topographical map of the area was created, along with the hazard map. Furthermore, the hazard levels for the surveyed area were also obtained: the largest area being medium (60.68%, 57.45%, 71.21% and 71.16 % respectively in Chattiwan, Bhawanchuli, Gurji and Hakpara), followed by high (32.59%, 18.91%, 17.03% and 11.54% respectively in Chattiwan, Bhawanchuli, Gurji and Hakpara), low (6.73%, 23.64%, 11.76% and 17.30% respectively in Chattiwan, Bhawanchuli, Gurji and Hakpara). It was also concluded that the forest area is at high risk followed by the bush and the settlement area in the first, second and the third site (Chattiwan, Bhawanchuli and Hakpara in order), and the cultivable land followed by the bush and the settlement area were found to be in high risks in order in the fourth site (Gurji).