피라미(Zacco platypus)의 난자형성에 관한 연구

장성재,김동희,류동석,등영건,Jang, Seung-Jae,Kim, Dong-Heui,Reu, Dong-Suck,Deung, Young-Kun 한국현미경학회 1995 Applied microscopy Vol.25 No.3

The development of pale chub oocyte from the immature oogonium to mature oocyte was investigated by light and electron microscope. The cytoplasm of pale chub oogonia was acidic and many vesicles were located at inner side of nuclear membrane. In primary oocytes, yolk vesicles were distributed in cytoplasm. Also, fibrous materials and protuberances were distributed on the surface of zona radiata. The nucleus of secondary oocyte was enlarged and yolk vesicles in cytoplasm migrated to zona radiata. In early egg, yolk mass are formed and yolk vesicles were located at inner side of zona radiata. Three-layered zona radiata was about $3{\mu}m$ in thickness. The three layers were an outer fibrous material layer, a middle nurse cell layer in which microvilli of early egg cytoplasm contact with processes of nurse cells, and an inner layer with high electron density. In mature egg, euchromatin and a germinal vesicle were developed, mitochondria, free ribosomes, and yolk mass were distributed in cytoplasm. But, yolk vesicles were disappeared. Specially, zona radiata of matured eggs were better thin than the one of immature eggs In conclusion, it is summerized that the oogenesis of pale chub were the increase of cell size, the formation and accumulation of yolk, the decrease in nucleat electron density, changes of zona radiata, and the development of microvilli.

Developmental Changes of the Oocyte and Its Enveloping Layers, in Micropercops swinhonis (Pisces: Perciformes)

Park, Jong-Young,Richardson, Ken-C.Richardson,Kim, Ik-Soo The Korean Society for Integrative Biology 1998 Korean journal of biological sciences Vol.2 No.4

In the goby Micropercops swinhonis, the development of its egg's enveloping layers could be divided into 4 stages. In the earliest developmental period, stage I, there is a simple oocyte surrounded by a layer of squamous follicular cells. Stage II corresponds to the yolk vesicle stage of vitellogenesis. Here the initial follicular layer has become bilaminar with the retention of its outer squamous cell layer and the acquisition of an inner cuboidal cell layer just over the zona radiata. The number and size of the cuboidal cells increases throughout this stage. Stage III corresponds to the yolk granule stage of true vitellogenesis. Here the cuboidal cells begin to be replaced by columnar cells. As the oocyte grows, the columnar cells increase in size. The columnar cells produce cytoplasmic neutral mucins and by the end of this stage their cytoplasm has been filled with this mucin. In stage IV a single layer of squamous cells still remained as the outer follicular layer of the oocyte. The secretory activity of the inner follicular layers' columnar cells has ceased and they had lost their cell wall integrity and ended as a series of bullet-shaped, neutral mucin deposits.

난막전구체 유전자발현을 이용한 내분비계장애물질 바이오마커 연구

박광식 동덕여자대학교 종합약학연구소 2002 동덕약학연구지 Vol.6 No.-

The fish egg envelope has been known to consist of two distinct layers. The outer layer is thin and is formed around the oocytes during the later stage of previtellogenic development. The inner layer, called zona radiata, is thicker than outer layer and occupies most of the egg envelope. Zona radiata proteins of medaka (Oryzias latipes) consist of two major subunit groups, ZI-1,2 and ZI-3. The precursors of ZI-1,2 and ZI-3 have been named choriogenin H and choriogenin L, respectively. They are synthesized in the liver in response to estrogen, and then released into the blood stream and incorporated into the zona radiata in sexually matured female medaka. However, choriogenin is also induced in male medaka when the fish are exposed to estrogenic chemicals. Choriogenin can be suggested as a possible biomarker of endocrine disruption in fish, however, relatively little has been known about chorogenin gene expression by estrogenic chemicals. In this study, full sequence of choriogenin L DNA was identified, and measurement of choriogenin mRNA induction was established in medaka by use of RT-PCR technique for the establishment of a simple tool for the screening of endocrine disrupting chemicals. And estrogenic effects of industrial chemicals were evaluated by this tool. When matured male medaka was treated with bisphenol A, nonylphenol, and 17-ethinylestradiol for 6 days, respectively, the expression levels of choriogenin L and H mRNA were elevated in treated groups. The induction of choriogenin subunits expression by these chemicals showed a dose-dependent pattern and choriogenin L was found to be more sensitive than choriogenin H.

韓國産 미꾸리에 關한 育種·繁殖學的 硏究 : Ⅵ. Histological Changes of Pituitary Gland and Oocytes from March to May Ⅵ. 계절에 따른 암컷의 腦下垂體와 卵母細胞의 組織學的 變化

姜京來,盧淳昌,尹鍾萬,朴弘陽,李相鎬 건국대학교 동물자원연구센터 1993 動物資源硏究誌 Vol.18 No.-

1992년 3월부터 1992년 5월까지 자연적인 채광상태에서 溫度를 유지시킨 후 채취된 미꾸리 암컷의 腦下垂體, 肝 및 卵巢 組織의 번식주기에 일어나는 미세구조의 변화를 광학 및 투과형 전자현미경을 이용하여 살펴보고, 이것들의 相互 聯關性을 알기 위하여 본 연구를 실시하였다. 본 연구에서 얻어진 결과는 다음과 같다. 성숙된 개체의 뇌하수체는 수많은 작은 분비과립과 커다란 분비구 및 불규칙한 형태의 과립들이 관찰되었다. 핵소체가 핵막 주변에 위치헤 있는 후기 단계(LMO)의 난모세포가 난황이 축적되는 성숙초기 단계(EMO)의 난모세포로 변형되는 시기를 난황의 형성이 개시되는 시기로 본다. 난모세포가 성장함에 따라 핵소체의 크기와 난황과립의 수가 증가하게 된다. 난황과립은 결정상의 과립으로 난세포질에 치밀하지 않은 상태로 축적된다. 성숙초기(EMO) 및 말기단계(LMO)에 난소는 커지고 투명하며 과립형태로서 색깔이 연노란색을 나타낸다. 따라서 난 내부를 쉽게 관찰할 수 있다. 방사대(ZR)는 성숙초기단계(EMO)에 단층으로부터 입방형태로 변화된다. 과립막 세포와 난모세포로 부터 유래된 미세융모는 난황형성이 진행되는 동안에 성장하게 되며, 방사대의 pore canals의 구멍을 통해서 서로 연결되어 있다. 따라서 외부로부터 물질의 이동이 있게 된다. 난모세포가 완전하게 성숙되어 방사대가 단단해 지면 미세융모는 그 기능이 상실된다. This study was undertaken to understand the development stages of pituitary gland, liver and ovary of Korean loach(Misgurnus anguillicaudatus) under natural circumstances from March to May 1922. Expecially, the light microscopic and ultrastructural changes of ooplasm and follicular membranes of oocytes, were observed by light and transmission electron microscope during the reproductive cycle. In pituitary gland of mature individuals, there were observed a number of secretory granules,large globules, and irregular masses.The vitellogenic phase began as these late perinucleolus oocytes became transformed into early maturing oocytes through the accumulation of yolk. The size of the nucleoli and number of the yolk granules increased as the oocyte growed. Yolk granules were loosely deposited in the oocyte as crystalline granules. Due to the presence of early and late maturing oocytes, their ovaries were enlarged transparent, granular and yellowish in color. Zona radiata change a squamous into cuboid shape in EMO stage. Microvilli from the granulosa cells and from the oocyte grow and make contact with each other in the pore canals of the zona radiata during vitellogenesis, but are withdrawn as the zona radiata becomes more compact during oocyte maturation.

좀구굴치 Micropercops swinhonis의 난여포층

박종영,김익수,이용주,Park, Jong-Young,Kim, Ik-Soo,Lee, Yong-Joo 한국어류학회 2001 韓國魚類學會誌 Vol.13 No.4

좀구굴치 성숙란의 난여포층은 외층인 theca cell과 내층인 granulosa cell로 구분되며 특히 원주형인 granulosa cell은 분비활동으로 인하여 세포질에 분비물이 축적되면서 난모세포를 둘러싸게 된다. 이러한 granulosa cell은 난황형성 초기인 난황포 시기에 입방형태를 보이지만 난황구시기에는 원주상세포로 바뀌면서 점액을 분비하는 특징을 보여 주고 있다. 이러한 부착물질은 형태가 없으며 전자밀도가 높다. 또한 이러한 분비물을 가지는 난막은 난황형성이 더욱 진행됨에 따라 동물극 부근이 식물극보다 더욱 두꺼워지고 커지게 된다. 이러한 점막여포층 아래에는 약 $7.8{\sim}11.5\;{\mu}m$ 두께의 방사대가 존재한다. 방사대는 전자밀도가 낮은 외층과 3~5층의 여러 전자밀도층을 가지는 내층으로 구성되어 있다. 한편 점막여포층은 방사대에 존재하지 않고 있기 때문에 이 물질은 난세포질이 여포상피로부터 기원된 것으로 생각된다. In the goby Micropercops swinhonis, the follicular layer of full-grown oocytes consists of an outer layer (theca cell) and an inner layer (granulosa cell). As the oocyte grows, columnar cells of inner granulosa layer secrete mucin to their cytoplasm and then surround the oocyte. Such granulosa cells appear to be cuboidal cells in the early vitellogenesis, yolk vesicle stage, to be replaced by columnar cell secreting mucins (adhesive materials) in the middle vitellogenesis, yolk granule stage. The enveloping layer of the oocyte has a muco- follicle layer filled with mucins. The mucins are an amorphous and electron-dense substance. Interestingly, the oocyte enveloping layer becomes thickened towards the animal pole as vitelogenesis proceeds. A zona radiata of about $7.8{\sim}11.5\;{\mu}m$ thick is present below the muco-follicle layer. The zona radiata is composed of an one-layered electron-dense externa and a three to five-layered electron-less interna.

發達 段階에 따른 韓國産 메기(Silurus asotus) 卵母細胞의 微細構造的 變化

윤종만,김계웅,신호철,장계남,류동석,박홍양 한국동물생명공학회(구 한국동물번식학회) 1995 Reproductive & developmental biology Vol.19 No.2

금강모치와 버들치 난모세포의 표피 구조

곽진영 ( Jin Young Gwak ),박종영 ( Jong Young Park ) 한국어류학회 2007 韓國魚類學會誌 Vol.19 No.1

A Microscopic Study on the Egg Envelope of an Endemic Korean Fish, Coreoleuciscus splendidus, Cyprinidae, Teleostei

Kim, Chi-Hong,Park, Jong-Sung,Kim, Jae-Goo,Park, Jong-Young Korean Society of Microscopy 2014 Applied microscopy Vol.44 No.3

Study on the egg envelope of an endemic Korean freshwater fish, Coreoleuciscus splendidus was carried out by light and scanning electron microscopes during its spawning season. The egg envelope on the entire egg is filled with plenty of long cylinder-like villi before and after fertilization. The fertilized eggs consist of two regions, the attaching and non-attaching parts to stick to the substrates. The villi covering the fertilized eggs have three kinds of villi in its length: 1) normal- sized villi of an average of $10.6{\mu}m$ on the non-attachment part; 2) longer and more slender villi over about $20{\mu}m$ on the attachment part, at least being two times longer than those of the non-attachment; and 3) shorter villi under about $5.0{\mu}m$ around the micropyle, with half the size of the non-attachment villi. The micropyle rotated in a counter-clockwise direction with five pieces of the ridges.

무지개 송어의 견전 육종학적 연구 IX. 난모세포의 성장 기간중 난포의 미세구조적 변화

윤종만,김계웅 한국통합생물학회 1993 동물학회지 Vol.36 No.2

1991년 8월부터 그이듬해 7월까지 채취된 무지개 송어(Oncorhvnchus mykiu) 난모세포의발달 단계를 이해하기 위하여 광필 및 전자현미경을 이용하여 본 각구를 실시하였다. 건국 대학교 축산대학 양수.장에서 수온이 14-16$^{\circ}C$이고 용존산소량이 7.6 $\pm$ 0.3ppm. PH 5.8 $\pm$ 0.2인 자연채광 상태인 1개의 10m3 탱크에서 N R.C. 사양표준에 준한 펠렛트 배합사료를 급여하여 연중 사육중인 체중 700-1,2009의 한복새끼인 무지개 송어 암컷 120마리를 공시희.로 이용 하였다. LPO(late perinucleolus oocyte), EMO(early maturing oocyte) 단계에서 과립막 세포와 협막세포와 같은 난포막 세포는 단층구조로 부터 입 방형구조로 변화되었고. 완전한 성장, 성숙 및 과숙기에 방사대(Eons rsdiat3)에 있는 pore canals의 입구가 봉쇄되었다. 방사대는 맨바깥목에 균일하면서 점은 두께의 막과 나선형의 두꺼운 복의 2개의 막으로 구성되어 있으며. 성숙기에 있는 난모세포에는 수초 및 자갈에 붙을 수 있는 온수성 어종 보다 상대적으로 얇은 점액성의 막이 그 주위를 둘러싸고 있다. 난모세포가 성장함에 따라 세포질 주변부 및 임립등 세포내에 막대기 형태의 미토콘드리아와 비대해진 내형질세강의 육가 급증하였다. 전자밀도가 높은 소포는 방사대에 인접한 난세포질 주변부에 많이 존재하며. 이는 성장중인 방사대의 pore Canals을 통해서 간으로 부터 합성된 단백질과 탄수화물을 수송하는 것과 밀접한 관계가 있는 것으로 사료된다. 자연적인 조건하에서 fP포막은 난형성 및 이에 영향을 미치는 성호르몬의 발달에 충분한 대르몬을 계절에 따라 분비시키는 데에 커다란 영향을 미치는 것으로 나타났다.