$^{99m}Tc$-HMPAO 표지효율에 대한 고찰

현준호,임현진,김하균,조성욱,김진의,Hyeon, Jun Ho,Lim, Hyeon Jin,Kim, Ha Kyun,Cho, Seong Uk,Kim, Jin Eui 대한핵의학기술학회 2012 핵의학 기술 Vol.16 No.2

핵의학검사에서 방사성의약품의 표지효율은 검사의 정확성과 신뢰성 측면에서 중요하다. 보통 Brain SPECT검사에 사용되는 $^{99m}Tc$-HMPAO는 화학적으로 불안정하고 불순물의 발생이 많아 표지효율의 저하가 나타나기 쉽다. 이에 본 연구는 $^{99m}Tc$-HMPAO의 표지효율에 영향을 미치는 인자에 대한 실험을 통해 $^{99m}Tc$-HMPAO 표지 및 사용에 도움이 되고자 한다. Brain SPECT에 사용되는 국산 동아제약의 HMPAO vial을 대상으로 실험하였다. 삼영사의 generator 55.5 GBq (1,500 mCi)를 이용하였고, TLC 측정세트(ITLC-SG, butanone, saline, TLC chamber)와 Radio-TLC 스캐너(Bioscan, AR-2000)를 이용하였다. 첫 번째 실험은 generator에서 1, 8, 16, 24, 28시간 간격을 두고 용출한 $^{99m}Tc$-pertechnetate를 각각 HMPAO와 표지하여 표지효율을 측정하였다. 두 번째 실험은 generator에서 용출한 $^{99m}Tc$-pertechnetate를 0, 1, 3, 6시간경과에 따라 1.85GBq (50mCi)/1 ml를 취하여 HMPAO와 표지하여 표지효율을 측정하였다. 세 번째는 $^{99m}Tc$-HMPAO를 표지 후 0, 30분, 3시간, 5시간, 7시간이 경과했을 때 표지효율을 측정하였다. 첫 번째 실험에서 표지효율은 시간 순으로 95.05%, 94.64%, 94.94%, 95.64%, 96.76%로 나타났다. 두 번째 실험에서는 94.38%, 94.23%, 93.26%, 91.03%로 나타났다. 세 번째 실험에서는 95.76%, 94.17%, 88.19%, 83.6%, 76.86%로 나타났다. 첫 번째 실험에서 generator에서 24시간 후에용출한 $^{99m}Tc$-pertechnetate를 사용 한 표지효율은 비교적 높게 측정되었다. 추가실험을 통하여 임상에서의 사용 가능성에 대한 논의가 필요할 것으로 사료된다. 두 번째 실험에서 시간이 지날수록 표지효율은 다소 감소하였으나 6시간 이후에도 91% 이상으로 높게 측정되었다. 보충실험을 통하여 보완이 필요할 것으로 판단된다. 세 번째 실험에서 시간이 흐를수록 표지효율은 급격하게 감소하였다. 특히, 정확한 검사를 위해 표지후 3시간 이내에 사용하는 것이 좋다고 판단된다. Purpose : The labeling efficiency of radiopharmaceuticals in nuclear medicine is important in terms of accuracy and reliability of the examination. Usually $^{99m}Tc$-HMPAO used for brain SPECT scan is chemically unstable since lots of impurities are existing. Therefore, occurrence of loss of labeling efficiency is easy to appear. In this paper, labeling and use of $^{99m}Tc$-HMPAO should be helpful through experiments on factors affecting the labeling efficiency of $^{99m}Tc$-HMPAO. Materials and Methods : Domestic HMPAO vials (Dong-A) used for brain SPECT scan were tested. Domestic Samyeong Generator 55.5 GBq (1,500 mCi), TLC measurement sets (ITLC-SG, butanone, saline, TLC chamber) and radio-TLC scanner (Advantest, Bioscan) were used. In the first experiment, after eluting generator at 1, 8, 16, 24, 28 hours apart, each eluted $^{99m}Tc$-pertechnetate were labeled with HMPAO and the labeling efficiency was measured. In the second experiment, after eluting $^{99m}Tc$-pertechnetate from a generator, $^{99m}Tc$-pertechnetate was drawn at 0, 1, 3, 6 hours. And each drawn $^{99m}Tc$-pertechnetate were labeled with HMPAO for measuring labeling efficiency. In the third experiment, labeling efficiency was measured at 0, 0.5, 3, 5, 7 hours after labeling $^{99m}Tc$-HMPAO. Results : In the first experiment, measured values were appeared 95.05, 94.64, 94.94, 95.64, 96.76% in passing order of time. In the second experiment, measured values were appeared 94.38, 94.23, 93.26, 91.03% in passing order of time. In the third experiment, measured values were appeared 95.76, 94.17, 88.19, 83.6, 76.86% in passing order of time. Conclusion : In the first experiment of this paper, labeling efficiency of $^{99m}Tc$-HMPAO labeled with $^{99m}Tc$-pertechnetate eluted after 24 hours from first elution. Additional experiments will be needed to discuss for usability. In the second experiment, the labeling efficiency was slightly decreased in chronological order, but it was measured higher than 90%. Also, additional experiments will be needed to discuss for usability. In the third experiment, the labeling efficiency was decreased considerably. Especially, within 3 hours after the labeling is recommended to use $^{99m}Tc$-HMPAO

Label-efficient Learning Methods for Computer Vision Applications

문예빈,오태현 대한전자공학회 2024 IEIE Transactions on Smart Processing & Computing Vol.13 No.2

In this work, we review the challenges of data scarcity and label inefficiency in deep learning and survey efforts to overcome these challenges. Many label-efficient learning methods have been proposed, but there is still room to develop more effective methods. We introduce potential yet promising directions to achieve label-efficient learning in terms of data, learning methods, and efficient use of prior knowledge. We also present case studies involving the latest methods.

편의점 도시락 레이블의 정보 중요성에 관한 연구

이연정,김승인 한국디지털정책학회 2020 디지털융복합연구 Vol.18 No.8

This study is about convenience store lunchbox labels for efficient information transmission. Its purpose is to identify the importance of the information that buyers want and to establish labels that can help buyers when purchasing lunchbox. The literature research and survey identified the factors that buyers consider important when purchasing lunchbox, analyzed the information identified on the label and the order of importance of the information. The study found that label information that lunchbox buyers think is important was in order of price, expiration date, calories, lunchbox components, and date of manufacture. Also, buyers who do not check labels pointed the less readable labels. This study expects that the information that buyers think is important will be newly established on labels so that buyers can easily check and purchase information through labels. 본 연구는 효율적인 정보전달을 위한 편의점 도시락 레이블에 관한 연구로써, 구매자들이 원하는 정보의 중요도를 파악해 도시락 구매 시 구매자들에게 도움을 줄 수 있는 레이블을 정립하는 데 그 목적이 있다. 연구방법으로는 문헌연구와 설문조사를 통해 구매자들이 도시락을 구매할 때 중요하게 생각하는 요소와 레이블에서 확인하는 정보 및 그 정보들의 중요도 순서를 분석하였다. 연구결과 도시락 구매자들이 중요하다고 생각되는 레이블 정보는 가격, 유통기한, 칼로리, 도시락 구성요소, 제조일자 순으로 나타났다. 또한, 레이블을 확인하지 않는 구매자들은 가독성이 떨어지는 레이블을 지적했다. 본 연구는 구매자들이 중요하다고 생각하는 정보들을 레이블에 새롭게 정립한다면 구매자들이 레이블을 통해 쉽게 정보를 확인하고 구매할 수 있을 것으로 기대한다.

디지털 전기천공시스템에서 형광 염료로 표지 된 DNA 전달 효율의 정량화

배서준 ( Seo Jun Bae ),임도진 ( Do Jin Im ) 한국화학공학회 2020 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.58 No.3

In the previous study, there was a big difference between the tendency of the delivery efficiency of Yo-Pro- 1 and the expression efficiency of the CFP gene, but there was a problem that could not provide a clue to this problem. Therefore, this study aimed to present a clue to this problem by quantifying and comparing the delivery efficiency after labeling DNA using a fluorescent dye, which was one of the methods for quantifying biomolecules. As a fluorescent dye for labeling, Yo-Pro-1 was used, and the delivery efficiency of the fluorescent dye Yo-Pro-1 and the labeled DNA was compared. The delivery efficiency of Yo-Pro-1 and labeled DNA according to the voltage condition of the digital electroporation system was maximized at 96 V, and the delivery efficiency tended to decrease as the voltage increased further. In the comparison of the delivery efficiency of Yo-Pro-1 and labeled DNA according to the number of voltage application conditions, the delivery efficiency was maximized at the number of 8 voltage application times for both delivery materials, and the delivery efficiency tended to decrease as the number of voltage application increases further. Through the two results, it was confirmed that the delivery efficiency using Yo-Pro-1 in the digital electroporation system represents the delivery efficiency of the system well. In addition, through the results of this study, the difference between the tendency of the delivery efficiency of Yo-Pro-1 and the expression efficiency of the CFP gene shown in the preceding study was not the result of the difference in the delivery efficiency of the delivery material, but it can be predicted to be due to a problem with the expression process of the genetic material that had been delivered.

대규모 정전사태 방지를 위한 에너지수요관리 법제도 개선 방안

김종천 ( Jong Cheon Kim ) 홍익대학교 법학연구소 2014 홍익법학 Vol.15 No.1

The September 15, 2011 blackout incident proves the fact that Korea`s electricity demand overweighs electricity supply during every year`s winter and summer festivals. Thus, public demands the government (Ministry of Commerce, Industry and Energy) to come up with more effective energy saving measures. The distorted price structure system that does not reflect current production price is highly criticized as being obstacles for energy-saving efforts. Wolsong nuclear power generator no.1 in Gyeongju has stopped due to malfunctioning in October and November of 2012. Also Yeonggwang Nuclear Power generator no.5 and no.6 in 1,000,000-kW class will be stop by the end of this year. During intensively cold period in this winter, Korea possibly will face a major blackout. In this respect, we must say that there is a limitation because existing regulation-oriented energy policy system cannot manage energy demand effectively. There are no applicable provisions regarding status of “public interest,” legal principles on the efficiency of Administrative law, and demand management, as constitutional basis and limit for energy demand management. Nevertheless, as constitutional basis and limit for energy demand management, policies regarding energy development can be derived (the Mining Law and Submarine Mineral Resources Development Act), energy demand management can be based on the Constitutional Law Article 37 Section 2. Since such legislation related to energy demand management policy may fall under restriction of fundamental rights, it should be consistent with principle of proportionality. However, regulating energy saving policy to energy consumer for energy demand management may constitutionally violate the individual freedom. For example, Rational Energy Utilization Act enforcement regulations Article 31 Section 2 requires that “standard for thermal limits of air conditioning and heating under Article 36 Section 2 Subsection 1(the “thermal limits”) are as follows: air conditioning: above 2 6℃ and heating: below 20 ℃, except for sales facilities and airport where air conditioning thermal limit shall be above 25℃.” In addition, requiring energy supplier to improve energy efficiency for energy demand management means limiting business occupation and therefore we should check whether it violates the principle of proportionality. It should be regarded as not conforming with principle of proportionality. As main content of Low Carbon Green Growth Act, there are fundamental principles such as energy policies (Article 39 Section 2), energy basic planning, and greenhouse gas energy target system. Building Act, for the purpose of energy demand management, legalized Green Building Certification Program, Intelligent Building Certification Program, vitalization of building environment friendly buildings and efficient energy usage of buildings, and Building Energy Efficiency Rating System (Energy Consumption Certification for real estate transactions). However, it was criticized for being under control of Ministry of Land, Transport and Maritime Affairs. This can be seen as the basis law for demand management program according to Rational Energy Utilization Act. In such law, there are many demand management systems such as energy use efficiency measure for national and provincial organizations and demand management investment plans of energy supplier, efficient energy consumption managing equipment labeling system, average energy consumption efficiency system and improvement order, standby power reduction product endorsing system, standby power reduction excellent product labelling system and preferential purchasing system, high efficiency energy equipment certifying system and preferential purchasing system, support system for energy service company, support system of companies which entered into agreements voluntarily, support system of energy management system, reporting system of energy glutton business and mandatory system of energy diagnosis, notification system of target energy consumption rate setting, utilization of waste heat, heat using machinery management - registration of certain heat using machinery, examination of machinery subject to inspection, appointment of operators for machinery subject to inspection, etc. Recently, developed countries have adopted several programs to strengthen energy demand management and they include: USA`s EERS, England`s EEC and CERT, EU`s efficiency management program for energy using machinery, Germany`s 2000 National Climate Protection Program enacted in 2005, Co2-building improvement and maintenance program enacted in 2001, Renewable Energy Law enacted in 2004, German Greenhouse Gas Emission Allowance Trading Act (TEHG) enacted in 2007 and Energy Saving Regulation (EnEV) enactment, quota law, and Japan`s “Top-Runner System” for energy use rationalization. These programs have been adopted for the purpose of energy saving and thus provide many implications to Korean legislation. Therefore, for energy demand management, “improvement on energy efficiency obligation” system was included to Rational Energy Usage Law Article 9 Section 2. Also, following improvement plans were suggested: Top-Runner System adoption plan, “energy conservation stickers on electronics” adoption plan (Section 65 Subsection 2), and “Energy Storage System (ESS) and smart grid (intelligence power grid) construction scheme” for Building and Promoting Smart Grid Act, enacted on May 24, 2011.

Re - 188 - DTPA ( Diethylenetriaminepentaacetic acid )의 표지와 생체내 분포

이진,이동수,김영주,장영수,정재민,신승애,정준기,이명철,고창순 ( Jin Lee,Dong Soo Lee,Young Ju Kim,Young Soo Chang,Jae Min Jeong,Seung Ae Shin,June Key Chung,Myung Chul Lee,Chang Soon Koh ) 대한핵의학회 1997 핵의학 분자영상 Vol.31 No.4

Re-188 is useful candidate for therapeutic radionuclide because it has a physical half life of 17 hours, contains beta ernissions suitable for therapy(maximum energy 2.12MeV) and emits a garnma ray that is suitable for quantitative diagnostic scanning(155keV). To use He-188 as a radionuclide compound of angioplasty balloon radiotherapy, we investigated the labelling method and biodistribution of Re-188- DTPA. We postulated that labeled Re-188-DTPA is preferable because it would be excreted via urinary system more easily than other compounds. To label Re-188 with DTI'A, 1ml of 222MBqI(6mCi) of Re-188 was added to DTPA solution(DTPA 20mg, SnC4 2HsO 10mg, pH 3.5) and boiled at 100C for 120min in water bath. pH was adjuted to 5 with 2.3Fo sodium acetate. I.abeling efficiency was measured using TLC-SG(acetone, saline). We evaluated biodistribution of Re-188-DTPA in sacrificed mice at 10 and 60 minutes after injection. We acquired images of kidneys, and drew tirne-activity r.urves in normal dogs and rats and calculated Trnax and Tl/2 in rats. The labelling efficiency was 95.7Yo on average. Labelling of Re-188-DTPA was stable(90% after 5hours) in vitro at room temperature. According to time-activity curves of dogs and rats, it took 15 to 20 minutes after injection for Re-188-DTPA to be washed out through kidneys. In conclusion, Re-188-DTPA was successfully labeled, Re-188-DTPA was stable in vitro and was excreted early via kidneys in animals. We could recornmend Re-188-DTPA as radionuclide of potential use in angioplasty balloon radiotherapy.

Preparation and characterization of Ga-68-deferoxamine to test the feasibility as a bifunctional chelating agent or a renal imaging radiopharmaceutical

김영주,이윤상,정재민 대한방사성의약품학회 2015 Journal of radiopharmaceuticals and molecular prob Vol.1 No.1

Chelating agents 1,4,7-triazacyclononanetriacetic acid (NOTA), 1,4,7,10-tetraazacyclododecane-1,4,7,10- tetraacetic acid (DOTA) and 30-amino-3,14,25-trihydroxy-3,9,14,20,25-penta-azatriacontane-2,10,13, 21,24-pentaone (desferrioxamine, DFO) were labeled with 68Ga and tested in vitro properties to check the feasibility of using DFO as a bifunctional chelating agent or renal imaging agent. The chelating agents of concentration 2 μM were labeled with 68Ga in 0.1 M HCl at pH 1.7-10.3 at room temperature and 80℃ and the optimal pH for labeling each chelating agent was found. And then, the chelating agents were labeled with 68Ga in various concentration of chelating agents at optimal pH. The labeled chelating agents were subject to stability test in human serum and to binding studies to human red blood cell (RBC) and plasma protein. The optimal pH’s of NOTA, DOTA and DFO for 68Ga-labeling were 4.4, 3.6 and 5.6, respectively. DFO (10 μM) showed high labeling efficiency (>97%) at pH 5.6. All the labeled chelating agents showed high stability in human serum. 68Ga-DFO showed low RBC binding but significant amount was bound to plasma protein. The results demonstrated that 68Ga-DFO can be used as a bifunctional chelating agent but not as a renal imaging agent. J Radiopharm Mol Probes 1(1):31-37, 2015

GCAM-EML을 이용한 대형상업용 건물에너지 효율변화의 장기영향 분석

백민호,ZULFIKAR YURNAIDI,오재익,김수덕 재단법인 에너지경제연구원 2015 에너지경제연구 Vol.14 No.3

While various energy efficiency improvement programs with specific energy saving targets are being proposed by governments, the feasibility of those programs and targets are properly assessed in quantitative manner. This research focuses on the enhanced building shell efficiency, efficiency improvement of lighting appliances to reduce cooling energy demand via 9 scenarios discussed in this article using GCAM-EML. Provided in this process of research are quantification of such policy impacts on energy system through scenario simulation results. The results show that this energy efficiency of large buildings are evaluated to be improved at maximum of 4.1% and 8.5% by 2030 and 2055 compared to the reference case. Although this improvement does not seem be large enough, it should be noted that the cumulative energy savings up to 2030 is estimated to be 2.36MTOE and it is 1.16 times of large commercial building energy consumption of 2010 reported by energy survey conducted on 2011. A further detailed research with a more realistic scenarios would better describe how various energy efficiency improvement programs currently deployed affect the overall building’s energy efficiency in the future, and this information could be better utilized for the detailed design of various ongoing energy efficiency programs. 건물에너지소비절약을 위한 정책들이 구체적인 소비저감목표를 제시하지만 저감목표달성이 어떻게 가능할 것인가에 대한 정량분석 결과를 제시하지 못하고 있다. 본 연구는 GCAM-EML모형을 이용하여, 대형상업용건물에 적용될 수 있는 건물외피 효율강화 및 조명기기의 효율개선 등 두 개의 요인에 대해 각각 세부 시나리오 분석을 통해 이들 두 개 요인이 대형 상업용건물에너지 효율개선에 미치는 효과를 살펴 봄으로써 건물에너지의 효율개선 잠재력을 평가해 본다. 두 개의 에너지효율개선 관련 요인을 근거로, 시나리오 분석을 진행한 결과 기준안 대비 효율개선은 2030년에 최대 4.1%, 2055년에 8.5%로 나타닜다. 이를 누적 에너지저감량으로 환산하면, 2030년 최대 2.36MTOE에 달하여 이는 2010년 대형건물의 총 에너지소비량의 1.16배에 해당한다여러가지 에너지효율개선 정책 중 대형상업용 건물에너지 소비의 극히 일부 * 이 논문은 2015년 자원경제학회 하계 학술대회에서 발표한 논문을 수정 보완한 논문입니다. 본 논문의 개선을 위해서 많은 조언을 하여 주신 에너지경제연구원 오경수 박사님, 그리고 익명의 심사자들께 감사드립니다. ** 아주대학교 에너지시스템학과 박사과정(주저자). minhobaek83@gmail.com *** 아주대학교 에너지시스템학과 석박사통합과정. viczhoel@yahoo.com **** 아주대학교 에너지시스템학과 연구교수. jaickoh@gmail.com ***** 아주대학교 에너지시스템학과 교수(교신저자). suduk@ajou.ac.kr 분을 차지하는 두 개의 요인만의 효과가 이러하다면, 다양한 에너지효율개선 정책이 향후 건축물의 에너지효율등급 개선에 줄 수 있는 잠재적 영향력을 짐작할 수 있게 한다. 한편, 본 연구에서 사용한 방법론은 개별정책의 효과를 통합평가 함으로써, 정책목표를 종합적으로 점검하는데 활용할 수 있을 것으로 기대한다.

Preparation and characterization of Ga-68-deferoxamine to test the feasibility as a bifunctional chelating agent or a renal imaging radiopharmaceutical

Kim, Young Ju,Lee, Yun-Sang,Jeong, Jae Min 대한방사성의약품학회 2015 Journal of radiopharmaceuticals and molecular prob Vol.1 No.1

Chelating agents 1,4,7-triazacyclononanetriacetic acid (NOTA), 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) and 30-amino-3,14,25-trihydroxy-3,9,14,20,25-penta-azatriacontane-2,10,13,21,24-pentaone (desferrioxamine, DFO) were labeled with $^{68}Ga$ and tested in vitro properties to check the feasibility of using DFO as a bifunctional chelating agent or renal imaging agent. The chelating agents of concentration $2{\mu}M$ were labeled with $^{68}Ga$ in 0.1 M HCl at pH 1.7-10.3 at room temperature and $80^{\circ}C$ and the optimal pH for labeling each chelating agent was found. And then, the chelating agents were labeled with $^{68}Ga$ in various concentration of chelating agents at optimal pH. The labeled chelating agents were subject to stability test in human serum and to binding studies to human red blood cell (RBC) and plasma protein. The optimal pH's of NOTA, DOTA and DFO for $^{68}Ga$-labeling were 4.4, 3.6 and 5.6, respectively. DFO ($10{\mu}M$) showed high labeling efficiency (>97%) at pH 5.6. All the labeled chelating agents showed high stability in human serum. $^{68}Ga$-DFO showed low RBC binding but significant amount was bound to plasma protein. The results demonstrated that $^{68}Ga$-DFO can be used as a bifunctional chelating agent but not as a renal imaging agent.

Energy-Efficiency Labels And Standards ; Global Assessment for Air conditioners

Jun Young CHOI(최준영) 대한설비공학회 2020 대한설비공학회 학술발표대회논문집 Vol.2020 No.6