HCC : PO-07 ; The comparison of radiologic findings and histopathology for the diagnosis of hepatocellular carcinoma

( Jin Nyoung Kim ),( Jae Young Jang ),( Soung Won Jeong ),( Jin Woo Choo ),( Soon Ha Kwon ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang Woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background/Aim: Detection and characterization of focal lesions in the liver is critical for screening patients with liver cirrhosis. The aim of this study was to investigate the sensitivity of magnetic resonance imaging (MRI) and spiral computed tomography (CT) for the diagnosis of hepatocellular carcinoma (HCC) and relation between radiologic findings and histopathology in HCC. Methods: One hundred four consecutive patients with HCC diagnosed by liver biopsy or hepatectomy were included. Radiologic findings such as CT and MRI were compared with histopathology. Typical radiologic finding of HCC was defined as early enhancement and early wash-out. Tumor cell differentiation was evaluated using Edmondson-Steiner criteria in liver cores. Results: Thirty one HCCs (29.8%) were grade 1 and 2, seventy three HCCs (70.9%) were grade 3 and 4. The sensitivity for detection of HCC was 83.7% for CT alone, 92.9% for MRI alone, 87.9% for both CT and MRI together, and 96% for either CT or MRI. Typical radiologic finding of HCC on CT has a tendency of well differentiated HCC (p=0.060), and typical findings of HCC on CT and MRI showed a significantly increased well differentiated HCC (p=0.013). Clinical variables were no significantly different according to the histopathology. Conclusion: Typical early enhancement and early wash-out pattern on CT and MRI showed a significantly increased well differentiated hepatocellular carcinoma on histopathology.

HCC : PO-07 ; The comparison of radiologic findings and histopathology for the diagnosis of hepatocellular carcinoma

( Jin Nyoung Kim ),( Jae Young Jang ),( Soung Won Jeong ),( Jin Woo Choo ),( Soon Ha Kwon ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang Woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background/Aim: Detection and characterization of focal lesions in the liver is critical for screening patients with liver cirrhosis. The aim of this study was to investigate the sensitivity of magnetic resonance imaging (MRI) and spiral computed tomography (CT) for the diagnosis of hepatocellular carcinoma (HCC) and relation between radiologic findings and histopathology in HCC. Methods: One hundred four consecutive patients with HCC diagnosed by liver biopsy or hepatectomy were included. Radiologic findings such as CT and MRI were compared with histopathology. Typical radiologic finding of HCC was defined as early enhancement and early wash-out. Tumor cell differentiation was evaluated using Edmondson-Steiner criteria in liver cores. Results: Thirty one HCCs (29.8%) were grade 1 and 2, seventy three HCCs (70.9%) were grade 3 and 4. The sensitivity for detection of HCC was 83.7% for CT alone, 92.9% for MRI alone, 87.9% for both CT and MRI together, and 96% for either CT or MRI. Typical radiologic finding of HCC on CT has a tendency of well differentiated HCC (p=0.060), and typical findings of HCC on CT and MRI showed a significantly increased well differentiated HCC (p=0.013). Clinical variables were no significantly different according to the histopathology. Conclusion: Typical early enhancement and early wash-out pattern on CT and MRI showed a significantly increased well differentiated hepatocellular carcinoma on histopathology.

Effects of dietary fish oil and trans fat on rat aorta histopathology and cardiovascular risk markers

Park, Seon-Hye,Park, Yong-Soon The Korean Nutrition Society 2009 Nutrition Research and Practice Vol.2 No.1

Fish oil and shortening have been suggested to have opposite effects on cardiovascular disease (CVD). This study investigated the effect of shortening and fish oil on CVD risk factors and aorta histopathology, and the association between risk factors and aorta histopathology. Male Wister rats (n=30) were fed an AIN-93G diet containing 20% fat in the form of fish oil, shortening, or soybean oil for 4 weeks. Total cholesterol (TC), triacylglyceride (TG), and C-reactive protein levels were significantly (P<0.001) lower in the fish oil than in soybean oil and shortening groups. HDL-cholesterol concentrations were significantly different (P<0.001) between groups. In addition, LDL-cholesterol levels were significantly (P<0.001) lower in the fish oil and shortening groups than in the soybean oil group. Insulin and glucose concentrations did not differ among groups. Effect of dietary fat on tissue fatty acid composition significantly differed in abdominal fat and brain compared with RBC, heart, kidney and liver. The aortic wall was significantly (P=0.02) thinner in the fish oil group than in the soybean oil and shortening groups. The aortic wall thickness was positively correlated with TG and TC, but negatively with EPA + DHA levels of all tissues. These results suggested that fish oil had protective effects on aorta histopathology by hypolipidemic action in this rat model.

Effects of dietary fish oil and trans fat on rat aorta histopathology and cardiovascular risk markers

Seonhye Park,Yongsoon Park 대한지역사회영양학회 2009 Nutrition Research and Practice Vol.3 No.2

Fish oil and shortening have been suggested to have opposite effects on cardiovascular disease (CVD). This study investigated the effect of shortening and fish oil on CVD risk factors and aorta histopathology, and the association between risk factors and aorta histopathology. Male Wister rats (n=30) were fed an AIN-93G diet containing 20% fat in the form of fish oil, shortening, or soybean oil for 4 weeks. Total cholesterol (TC), triacylglyceride (TG), and C-reactive protein levels were significantly (P<0.001) lower in the fish oil than in soybean oil and shortening groups. HDL-cholesterol concentrations were significantly different (P<0.001) between groups. In addition, LDL-cholesterol levels were significantly (P<0.001) lower in the fish oil and shortening groups than in the soybean oil group. Insulin and glucose concentrations did not differ among groups. Effect of dietary fat on tissue fatty acid composition significantly differed in abdominal fat and brain compared with RBC, heart, kidney and liver. The aortic wall was significantly (P=0.02) thinner in the fish oil group than in the soybean oil and shortening groups. The aortic wall thickness was positively correlated with TG and TC, but negatively with EPA + DHA levels of all tissues. These results suggested that fish oil had protective effects on aorta histopathology by hypolipidemic action in this rat model.

비용, 상악동 및 하비갑개 조직의 병리조직학적 소견의 비교

한길수,박인서,이동율,김창효,오윤석,장태영 대한비과학회 2008 Journal of rhinology Vol.15 No.1

Background and Objectives:The nasal polyp is the most common form of mass lesion in the nose with an unclear etiology and pathogenesis among patients in Asia. Also, the pathological characteristic of nasal polyps in these patients is eosinophil infiltration. These findings, as reported in Asia, are distinct from those of the West. Moreover, the role of eosinophil is recently found to be on the increase even in Asia. So, we investigated the proportion of eosinophil infiltration in the nasal polyp among patients in Korea. Materials and Methods:The study group consisted of 35 patients with a mean age of 43 years (ranging from 6 to 72 years). Tissue samples were taken via endoscopic sinus surgeries from the nasal polyp, inflammatory sinus mucosa, and the inferior turbinate. We compared 5 parameters of mucosal histopathology. Result:The incidence of a severe eosinophilic infiltration in the nasal polyp was 70%. Nasal polyp and inflammatory sinus mucosa had a larger eosinophil, lymphocyte and polymorphonuclear leukocyte infiltration than a normal nasal mucosa. Also, the nasal polyp and sinus mucosa had a more severe basement membrane thickening and goblet cell hyperplasia. Distinctively, the nasal polyp had a severe eosinophilic infiltration and a more thickened basement membrane. Conclusion:The present histopathologic feature of the nasal polyp among Koreans indicates that cases of eosinophilic nasal polyp seems to be on the increase. Background and Objectives:The nasal polyp is the most common form of mass lesion in the nose with an unclear etiology and pathogenesis among patients in Asia. Also, the pathological characteristic of nasal polyps in these patients is eosinophil infiltration. These findings, as reported in Asia, are distinct from those of the West. Moreover, the role of eosinophil is recently found to be on the increase even in Asia. So, we investigated the proportion of eosinophil infiltration in the nasal polyp among patients in Korea. Materials and Methods:The study group consisted of 35 patients with a mean age of 43 years (ranging from 6 to 72 years). Tissue samples were taken via endoscopic sinus surgeries from the nasal polyp, inflammatory sinus mucosa, and the inferior turbinate. We compared 5 parameters of mucosal histopathology. Result:The incidence of a severe eosinophilic infiltration in the nasal polyp was 70%. Nasal polyp and inflammatory sinus mucosa had a larger eosinophil, lymphocyte and polymorphonuclear leukocyte infiltration than a normal nasal mucosa. Also, the nasal polyp and sinus mucosa had a more severe basement membrane thickening and goblet cell hyperplasia. Distinctively, the nasal polyp had a severe eosinophilic infiltration and a more thickened basement membrane. Conclusion:The present histopathologic feature of the nasal polyp among Koreans indicates that cases of eosinophilic nasal polyp seems to be on the increase.

성상세포교종에서의 BUdR, AgNOR's, PCNA에 대한 비교연구

이원창,신문수,정남,허승곤,이혜경 대한신경외과학회 1993 Journal of Korean neurosurgical society Vol.22 No.12

In 14 cases of astocytoma, we performed a comparative study of BUdR, AgNOR's, and PCNA with histopathology. Fourteen astrocytomas were classified histopathologically into low grade, anaplastic, and G.M.(glioblastoma multiforme) groups. These comprised 3 G.M., 5 anaplastic astrocytomas, and 6 low grade astrocytomas. The mean BUdR-LI(Labeling Index) was statistically different(P<0.005) between G.M.(10.4), anaplastic astrocytoma(3.6), and low grade astrocytoma(1.3). On the basis of correlation between BUdR-LI and histopathology which was reported in 1989 at PMC, we examined the relationship between BUdR-LI and AgNOR's count, and between BUdR-LI and PCNA-LI, Both AgNOR's count and PCNA-LI did not correlate with BUdR-LI, and also not with histopathology. This study indicates that, at present, for prognosis and differentiation between malignant and benign astrocytoma, histopathlogy and BUdR-LI are important.

드렁허리, Monopterus albus의 구두충 감염예에 대한 병리조직학적 관찰

강혜민 ( Hye Min Kang ),이한나 ( Han Na Lee ),임상구 ( Sang Gu Yim ),김영대 ( Young Dae Kim ) 한국어병학회 2013 한국어병학회지 Vol.26 No.3

2013년 3월부터 국립수산과학원 내수면양식연구 센터에서는 종 보존 연구를 위해 1,000미의 드렁허리(Monopterus albus)를 양성하고 있었다. 그러던 중에 100미의 드렁허리에서 체색흑화, 점액과다, 항문돌출과 같은 임상증상을 보였으며 몇몇 개체들은 폐사하였다. 해부 검사한 결과 100미 전부 구두충에 감염된 것을 확인할 수 있었다. 이에 드렁허리에 감염된구두충을 병리조직학적으로 관찰하였다. 구두충은 소화관 전반에서 관찰되었으며 개체 당 평균 19미의 충체를 확인할 수 있었다. 중감염된 개체에서는 구두충에 의한 장폐색 증상을 보였으며 충체는 소화관점막하층에 proboscis를 침투시켜 기생하고 있었다. 특징적으로 위와 장에서 점막상피내 충혈과 점막하조직내 호산성 세포의 침윤, 염증반응, 기생충성 육 아종 소견을 보였다. 일부 개체에서는 위선상피의 공포화와 장 점막층의 괴사도 관찰되었다. 소화관 외기타장기에서는 특징적인 병변은 관찰되지 않았다. 본 연구로 드렁허리에 감염된 구두충의 감염정도를 알 수 있었고, 향후 드렁허리 양식에 큰 도움이될 것으로 보인다. Since March in 2013, Inland Aquaculture Research Center, NFRDI has cultivated 1,000 wild swamp eel(Monopterus albus) for species conservation research. While cultivating, 100 fishes showed clinical sign that darkness color, mucus hypersecretion and anus rubor. Even some of them were died. Result of anatomical test, all 100 fishes were infected with intestinal parasite, acanthocephalan. So we were going to determine the case of acanthocephalans infection in swamp eel(Monopterus albus) as histopathologically. Acanthocephalan was founded in alimentary canal only. Parasite were confirmed 19 unit in individual fish, averagely. Heavy infected fishes were confirmed enterocleisis by acanthocephalans. Worms were attachment in submucosa layer of alimentary canal by invading proboscis. Characteristic symptoms were observed in stomach and intestine, including hyperemia in mucous epithelium, infiltration of eosinophills in submucosa layer, inflammation, parasitic granuloma. Some fishes showed vacuolization of gastricgland epithelium, necrosis of intestinal mucosa. Other organs, excluding alimentary canal, were not found lesion. The results of this study, the effect of the acanthocephalan infection on swamp eel(Monopterus albus) could find and it seems to be a big help in the future swamp eel(Monopterus albus) cultivation.

Histopathological features and viral genome detection in caprine arthritis encephalitis virus infected dairy goats in Korea

( Gain Son ),( Eun-sang Cho ),( Hyun-jin Shin ),( Hwa-young Son ) 한국가축위생학회 2017 韓國家畜衛生學會誌 Vol.40 No.3

Caprine arthritis encephalitis (CAE) virus is a causative agent of caprine arthritis-encephalitis. In our previous study we reported a prevalence of CAE. In this study, we described the further detailed pathological features of CAE and examined the detection of virus by in situ hybridization (ISH). Histopathologi-cally, interstitial pneumonia and bronchopneumonia in lung, focal inflammation in mammary glands, perivascular cuffing in brain, arthritis, and focal necrosis, mild steatosis, inflammatory cell infiltration of liver were noted. CAEV proviral-DNA was identified by nested polymerase chain reaction (PCR) in blood cells, brain, synovial fluid, and lymph node. Confirmation by nested PCR involved amplification of a 296 bp (1^st PCR) and 185 bp (2^nd PCR) fragments corresponding to a conserved region on the gag gene of CAEV. Positive ISH signals were detected in the brain and liver. In conclusion, significant histopathological findings included parenchymal infection in various organs, including the lung, liver, brain, joint, and mammary gland were noted in the CAEV infected dairy goat. ISH can help confirm the diagnosis of CAE in formalin-fixed samples.

Equisetum arvense L.이 Streptozotocin유발 당뇨성 쥐의 항당뇨작용에 미치는 영향

이현자,김석환,손기호,최종원 慶星大學校 環境問題硏究所 1993 環境硏報 Vol.3 No.1

The present study was undertaken in order to elucidate the effects of pretreatment with Equisetum arvense L.(EM) on change in serum lipid compositions and pancreatic enzymes induced by streptozotocin(STZ) in rats with pancreatic histopathology. 1. Total serum lipid and triglyceride levels in the STZ-induced diabetic rats were significantly higher than those in the control group, and those in the group pretreated with EM but the phopholipid serum level was not significantly different in any of the group. 2. In the STZ-induced diabetic group, the total serum cholesterol, VLDL-and LDL-cholesterol levels, and the atheroscerotic index were higher, while the HDL-cholesterol level was lower when compared to the control group. HOwever, these changes were slightly prevented by EM. 3. Pancreatic lipase and trypsin activities were increased, but amylase activity was decreased by STZ, and pretreatment with EM slightly prevented these STZ-induced changes, in the histological changes of the pancreatic β-cell. These results suggest that EM pretreatment slightly supresses STZ induced changes in the serum lipid compositions and pancreatic enzyme via maintenance of prevent of β-cell destruction by STZ.

고충증 ( Sparganosis ) 의 임상 및 병리조직학적 고찰

이범주(Beom Joo Lee),안성구(Sung Ku Ahn),김수찬(Soo Chan Kim),이승헌(Seung Hun Lee) 대한피부과학회 1992 大韓皮膚科學會誌 Vol.30 No.2

We reviewed the clinical epidermiologic features and skin biopaies of 23 patients who were diagonosed with sparganosis. Clinically, the parasites were obtained from the lesions and confirmed histopathologically. The purpose of this study was to evaluate the elinical, epidemiological, and histopathological charaeteristics of sparganosis. The results are summurized as follows : 1. There were no difference between male (48%) and female (52%) patients. 2. Age distribution, at first visit, were variable, ranging from 7 to 75, with the mean age of 40 years-old. 3. Duration of symptoms were variable, ranging from 10 years to 15 years, with a mean duration of 3 years. 4. Frequency of clinical features were as follows; movable or fixed subcutaneous nodule (16 cases), subcutaneous nodule with pain & focal warmth t.o touch (6 cases), seizure (I case). 5. Number of parasites per lesion were single lesion with single, parasite (21 cases), single lesion with two parasites (2 cases) and three parasites (3 case). 6. Frequency of location of lesion were abdominal wall (8 cases), thigh (4 cases), breast (3 cases), scrotum (3 cases), arm (3 cases), buttoek (1 cases), ciiest wall (1 case), brain (1 case). 7. The histological change of the affected tissue were characterized as follows ; 1) necrotizing and granulomatous tissue with or without parasif os in the lesions. 2) some cases were associated with marked fibrosis or formation of lymphoid follicles. 3) There were many lympho-histocytes, eosinophils, giant cel1s and some plasma cells near the lesions. (Kor J Dermatol 1992;30(2): 168-174)