Enhanced CNN-based Plant Growing-stage Classification using Additional Information Carried in an Additional Channel

Yeh-Huann Goh,Zhao-Jun Ho,Chee-Ho Ng,Yann-Ling Goh 대한전자공학회 2019 IEIE Transactions on Smart Processing & Computing Vol.8 No.3

Studying the observable characteristics of mutants and the growing stages of the same genotype plant interacting with various environmental conditions is important in order to understand the performance of a particular trait in different growth environments. A plant"s growing stage affects the growth rate of leaves, the photosynthetic rate, water absorption capacity, and other characteristics. By automating the plant mutant classification process and the growing-stage classification process, botanists and agriculture scientists can perform large-scale experiments to cultivate plants with useful traits to combat extreme environmental conditions. This research aims to construct an enhanced optimum convolutional neural network (CNN) for image-based plant growing-stage classification, and a description of the algorithms to construct the optimum CNN for image-based plant mutant classification is included as well. This research was carried out using the Ara2013-Canon dataset annotated by the International Plant Phenotyping Network (IPPN) for classification processes. Optimum parameters found in this paper are for 1) the number of convolutional layers, 2) the number of neurons in a fully connected (FC) layer, and 3) the number of FC layers in a CNN for plant growing-stage classification. The possibility to enhance the successful classification rate is explored by introducing an additional channel that carries additional useful information, such as 1) mutant type, 2) number of leaves, 3) total size for all leaves, 4) mean leaf size, and 5) standard deviation of the leaf size in the form of square matrices. Experimental results show that under optimum conditions for growing-stage classification, a CNN classification system utilizing plant images and all additional useful information provides the best recognition rate at 81.97%.

4년생 인삼의 수확시기에 따른 ginsenoside 및 일반 화학성분의 변화

안영남,이선영,정명근,최강주,강광희 韓國作物學會 2002 한국작물학회지 Vol.47 No.3

4년생 인삼의 저장근에 함유된 일반 화학성분, 유리당 및 사포닌 성분을 수확 시기별로 조사하고 그 변화 양상을 검토하여 4년생 인삼의 최적 수확기 선정을 위한 기초자료 제공을 목적으로 한 본 실험의 결과를 요약하면 다음과 같다. 1. 4년생 인삼의 전 생육기간 중 조단백 함량은 지상부 출현기인 4월이 20.77%로 가장 높았고, 지상부 전개가 완료된 6월이 13.13%로 가장 낮았으며, 8월 이후에는 17% 수준으로 함량의 변화가 일어나지 않았다. 2. 조지방 및 조섬유 함량은 조단백과 반대의 양상으로 개화기인 5월에 최대치를 나타내었다. 3. 총당은 전 생육기간 중 60-71%의 함량범위로 존재하여 전체성분 중 가장 높은 함량을 나타내었고, 지상부 생육이 완료된 6월이 71% 수준으로 가장 높았으며, 개화기인 5월이 60% 수준으로 가장 낮았다. 4. 인삼 저장근의 유리당 함량은 지상부 출현기인 4월에 20.40%로 최대치를 보였으나, 개화기인 5월에는 11.89%로 전생육시기 중 최소의 함량을 나타내었다. 5. 유리당 중 가장 함량이 높은 sucrose의 변화(10.96-19.60%) 양상은 전 수확기간 중 총 유리당의 변화(11.89-20.40%) 경향과 유사한 양상을 나타내지만 fructose의 함량 변화와는 서로 상반된 양상을 나타내줬다. 한편 유리당 중 glucose와 maltose의 함량은 전 수확기간 중 통계적 차이가 인정되지 않았다. 6. 조사포닌과 총 ginsenosides함량은 개화기인 5월에 각각 7.60% 및 4.09%로 기타 수확시기에 비해 월등히 높았으며, 그 외의 시기에는 통계적으로 유의한 차이가 인정되지 않았다. 따라서 4년생 인삼에서 성분적 특성만을 고려할 경우 개화기인 5월이 가장 유리하였다 그러나 개체당 함량은 10월이 가장 높게 나타났다.다소 감소되었으며 건답직파에서는 5년연속 재배시 62%로 크게 감소되었다.다.0일이었다.환경적응성이 낮은 경향이었다.은 모두 경운 유·무에 관계없이 시비수준이 높을수록 높은 경향을 보였고, 10일에 최고수준을 보인 후 완만한 감소를 나타냈으며, K+, Ca2+에 비해 Mg2+이 보다 낮은 함량을 보였다.989년산 일반계를 10분도와 12분도로 도정하였을 때 도정도에 따른 밥맛의 차이는 없었다.X>CoOx는 Co3O4 로 존재하고, 반응 전의 경우에는 이와는 다른 chemical state를 보여주었다. XRD 및 XPS 결과를 바탕으로, 촉매표면에 존재하는 Co3O4 의 외부표면이 Co2TiO4 와 CoTiO3 같은 CoTiOx 로 encapsulation되어 있는 모델구조를 제안할 수 있고, 이는 반응시간의 함수로 나타나는 촉매활성에 있어서 전이영역의 존재를 잘 설명할 수 있을 뿐만 아니라, XRD와 XPS에서 얻어진 촉매의 물리화학적인 특성을 잘 반영할 수 있다. 나타냈고, 골격근과 눈 조직에서 피루브산에 대한 LDH의 친화력이 상당히 크므로 LDH가 혐기적 조건에서 효율적으로 기능을 하는 것으로 사료된다.5) and "Cleanliness of clothes & features" (p <0.05) of VIP ward were significantly higher than those of a general ward.tive to apply.아울러 고려(考慮)해야 한다. 이것은 고무기술자(技術者)가 당면(當面)해야할 과제(課題)에 속(屬)하며 바람직 한것은 본장( This study was conducted to know the change pattern of chemical components, especially proximate constituents, saponin and free sugars in roots of 4-year ginseng according to different harvesting time, and to find out the optimum harvesting time on the basis of chemical components in fourth year ginseng root. The crude protein content was 20.77% of the highest on the April (shootinzg stage),13.13% of the lowest on the June among all growing stages, and was constant at 17% after the August. But crude oil and fiber contents showed the highest value on the May (flowering stage). The content of total sugars which was the largest constituent among the chemical components in ginseng root was in the range of 60~7(1% during the all growing stages. It showed the highest value of 7l% on the June, but the lowest of 60% on the May. The total free sugar contents was the highest on the April (20.40%), but the lowest on the May (11.89%). The change pattern of sucrose contents (10.96-19.60%) was same as the total sugars contents (11.89-20.40%), on the other hand, the contents of glucose and maltose were not changed significantly during all harvesting times. The contents of crude saponins and total ginsenosides had the value of 7.60% and 4.09% on the May, respectively. That was statistically significant, but the other harvesting times were not. Therefore, on the basis of the only chemical characteristics in the four year-old ginseng root, we suggest that the harvesting on the May at flowering stage was most proper time.

Identification of Differentially Expressed Proteins at Four Growing Stages in Chicken Liver

Lee, K.Y.,Jung, K.C.,Jang, B.G.,Choi, K.D.,Jeon, J.T.,Lee, J.H. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.10

Because of high growth rate and large deposition of fat in the abdomen, the chicken has been used as a model organism for understanding lipid metabolism, fattening and growing. In this study, differentially expression of proteins in chicken liver, one of the important organs for lipid metabolism, has been investigated at four different growing stages. After separation of proteins using two-dimensional electrophoresis (2-DE), more than 700 protein spots were detected. Among them, 13 growing stage specific proteins in chicken liver were selected and further investigated by matrix-assisted laser adsorptions ionization-time of flight mass spectrometry (MALDI-TOF MS). Of these, 12 proteins were matched to existing proteins based on a database search. The identified fat-related proteins in this study were fatty acid synthase (FASN) and malic enzyme (ME1). These proteins were more highly expressed at week 32 than at other weeks. In order to confirm the differential expression, one of the proteins, FASN, was confirmed by western blotting. The identified proteins will give valuable information on biochemical roles in chicken liver, especially for lipid metabolism.

황갈색 재래닭의 간에서 성장 단계별 차등 발현 유전자 분석

이경연,유성란,정기철,장병귀,최강덕,이준헌,Lee, K.Y.,Yu, S.L.,Jung, K.C.,Jang, B.K.,Choi, K.D.,Lee, J.H. 한국가금학회 2007 韓國家禽學會誌 Vol.34 No.2

한국 재래닭에서 성장에 따른 유전자들의 발현 변화를 알아보고 성장 촉진, 대사 및 면역 관련 유전자를 발굴하기 위하여 주령별로 닭의 간에서 RNA를 추출하였으며 10개의 arbitrary ACPs를 이용하여 차등 발현되는 유전자를 조사하였다. 발현량에 현저한 차이를 보이는 5개의 유전자들이 선별되었으며, 이 중 3개의 유전자들은 BLAST search 결과 이미 기능이 알려진 FTH1, SAA와 HSP90B1으로 밝혀졌다. 그러나 2개의 유전자들은 닭의 genome sequence가 끝났음에도 불구하고 기능이 밝혀져 있지 않아 앞으로 이 유전자들의 기능에 대한 연구가 지속되어야 함을 의미한다. 본 연구에서 닭의 간에서 성장 단계별로 발현 차이를 보이는 유전자들은 앞으로 다른 유전자와 단백질들과의 관계를 통하여 닭의 성장 및 지방 대사를 이해하는데 도움을 줄 것으로 사료된다. The chicken liver has been involved in various biological functions including detoxification, glycogen storage and plasma protein synthesis. The aim of this study was to investigate differentially expressed genes in chicken liver in four different growing stages. Using 10 arbitrary Annealing Control Primers (ACPs), five differentially expressed genes have been identified. Based on the Basic Local Alignment Search Tool (BLAST) search results, three of them were matched with previously known genes, and the other two were matched with unknown EST sequence and a hypothetical protein, respectively. In order to confirm the expression results, quantitative real-time PCR was also performed. The high similarities between the expression data using arbitrary ACPs and quantitative real-time PCR indicate that the identified genes are the real differentially expressed genes in different growing stages. The genes identified in this study can be used as valuable biomarkers in chicken with further investigation of the functions.

생육시기에 따른 느타리류의 자실체 특성 변화

류재산,나경숙 한국버섯학회 2023 한국버섯학회지 Vol.21 No.4

The characteristics and spore production of Gonji7ho, Bunhong, and Sunjung fruiting bodies were assessed at different growth stages. The shape of the Pleurotus species fruiting body starts out short and small, then takes on a typical mushroom shape as it grows. Gonji7ho has a long stalk, Bunhong has a short stalk and a wide cap, and Sunjung's cap and stalk dimensions are intermediate. Each variety displayed deep color at the beginning of growth but became steadily lighter with continued growth. The shape of the linkage between the mushroom stalk and cap changed from an initial central position to a lateral position after the growing stage. Gonji7ho cap diameter increased 7-fold from 15.5 mm (5 days of growth) to 37.9 mm (9 days of growth). Growth rates for each growth day measured using the growth percentage of the previous day were 285.5% (5 → 6th day), 182.2% (6 → 7th day), 129.4% (7 → 8th day), and 103.8% (8 → 9th day). This trend was also observed in Bunhong and Sunjung, but Bunhong’s growth rate was more rapid (4.9 fold on day 6, 2.7 fold on day 7) and continued to increase through day 9. Harvest yield, which is of greatest interest to farmers, displayed a similar trend spanning the growth period, as did cap diameter. Gonji7ho harvest yield increased rapidly until day 7 of growth (more than 177%), then growth slowed down beginning around day 8, and further decreased on day 9 (98%). Similar trends were observed in Bunhong and Sunjung. Bunhong showed characteristic rapid growth in harvest yield (4.9 fold compared to the previous day on day 6 and 2.7 fold on day 7), and the increase continued through day 9. A decrease in mushroom harvest yield commonly seen in the late growth stage is thought to be due to the death of some mushrooms and decomposition of cap tissue. Basidiospore content increased with number of growth days but decreased after day 8. Gonji7ho yielded the highest production on day 7 of growth, coinciding with harvest time, with 209,000,000 spores. This trend was also observed in Bunhong and Sunjung. These results will provide researchers with basal data and guide farmers in selecting the optimal harvest day.

4. Effect of Harvest Date on Agronomic Characteristics, Forage Quality and Yield of Rye

Guoqiang Zhao,Chang Liu,Hakjin Kim,Jong Geun Kim 한국초지조사료학회 2018 한국초지조사료학회 학술대회논문집 Vol.2018 No.09

In this experiment comparison was made for the agronomic characteristics and quality of forage rye among the different growing stages. Rye was harvested every 5 days, then separated into three parts and determined the quality. Before harvesting, plant height and yield were recorded. Dry matter yield increased continuously, while fresh matter yield decreased after heading. ADF and NDF value increased before blooming and then showed a slight fluctuate. In addition, highest CP content appeared on jointing stage. TDN and RFV went down until blooming stage and then showed a steady trend. So the proper harvest time is from heading to blooming stage for forage rye.

작약 생육시기에 따른 약근수량 및 Paeoniflorin함량 변화

姜光熙,鄭名根 韓國作物學會 1994 Korean journal of crop science Vol.39 No.4

경북 영천군 독농가가 재배한 정식 4년차인 의성종과 밀양종(P. lactiflora Pall.에 속하는 지방종)작약을 공시재료로 하여 생육시기별로 paeoniflorin 및 내용성분을 조사하고 수확기별로 약근수량을 조사한 결과를 요약해 보면 1. 개화기인 5월에 저장근 건물비율은 약 23%로 가장 낮았고, 수축율은 47%로 가장 높아 서로 상반된 양상을 나타내었으며, 6월 이후는 이들 특성의 변화가 없어 안정적이었다. 2. 전 생육기간 중 내용성분의 변화가 가장 큰 시기는 개화기인 5월이었으며, 약근에 함유된 paeoniflorin, 조단백, 조섬유 및 조회분은 공통적으로 개화기인 5월에 함량이 일시적으로 증가하였고, 총당 및 조지방은 변화가 적었다. 3. Paeoniflorin은 5월 시기에 6.37%로 가장 높았으며 6월 이후 변화없이 안정된 경향을 보였고, 의성종과 밀양종의 품종간 차이는 뚜렷하여 밀양종이 약 2% 높았다. 4. 전 생육기간 중 내용성분의 변화가 심하였던 전기(2∼5월)와 안정적인 후기(6∼10월)의 특성간 상관관계 중 전·후기 모두 정의 유의상관을 가진 것은 전분과 건물중, 조섬유와 paeoniflorin이며 부의 유의상관을 가진 특성은 전분과 조섬유, 전분과 paeoniflorin, 전분과 건물중 이었다. 5. 경장, 주당경수, 건전경비율은 이병으로 인한 고사로 8월보다 10월 수확기에서 현저히 낮았으며, 약근수와 길이도 근부현상이 심하여 10월 수확기에서 감소되었다. 6. 4년생 작약의 수확적기는 8∼9월이 적당하며 지상부 고사가 심하면 수확기를 앞당기는 것이 수량 및 품질면에서 더욱 안정적이다. This experiment was conducted to determine best harvesting time and observe paeoniflorin and some chemical component concentrations of 4-year-old paeonia radix in Euisung and Milyang varieties. Concentrations of paeoniflorin and some chemical components were investigated at 8 periods from Feb. to Oct. Yield of paeonia radix was investigated in Aug. 19, Sep. 18 and Oct. 17. Paeoniflorin and some chemical components were changed seriously at May as flowering time and stabilized after June. The lowest dry matter ratio was 23% and the maximum shrinkage ratio was 47% in May during all the growing stages. Especially, the difference of paeoniflorin concentrations between Euisung and Milyang was about 2%, and paeoniflorin concentrations in Milyang were higher than in Euisung variety during all the stages. Serious infection of disease in October, stem length, stem number per stock and healthful stem ratio in October were more reduced than in August. Yield of paeonia radix was not different from August to September, but that of October harvest was obviously reduced by root-decay-disease. Reasonable harvesting time in 4-year-old paeonia radix was late August to mid-September. When infected shoot rate by disease were severely high, early harvest was more stable in the yield and quality aspects.

Effects of Renewal Pattern of Recycled Nutrient Solution on the Ion Balance in Nutrient Solutions and Root Media and the Growth and Ion Uptake of Paprika (Capsicum annuum L.) in Closed Soilless Cultures

Myat Thaint Ko,Tae In Ahn,Jong Hwa Shin,Jung Eek Son 한국원예학회 2014 원예과학기술지 Vol.32 No.4

Ion imbalance in recycled nutrient solutions is caused by selective ion uptake of plants, which occurs at different rates in different growth stages. The objectives of this study were to investigate the ion balances in both recycled nutrient solutions and rockwool media using different renewal patterns for the nutrient solutions, and to analyze the subsequent effects on uptake of water and nutrients. Over 12 weeks of paprika cultivation, two different renewal patterns (week units) of 6-4-2 and 8-2-2 weeks were compared with a constant renewal pattern of 4-4-4 weeks (control). The nutrient solution in the reservoir tank was constantly maintained at EC 2.5 dS·m<SUP>-1</SUP> and pH 5.5-6.5. The changes in the ion balance with the 4-4-4 week pattern were smaller than those with the other treatments. In the early growth stage, however, the ion balances similarly changed among all treatments. Greater changes were subsequently observed for the 6-4-2 week pattern. Although fruit yield and shoot fresh weight of paprika were the lowest with 6-4-2 renewal pattern, no significant differences were observed. Our results indicate that renewal intervals can be extended in consideration of growth stage for more efficient and practical operations in closed soilless cultures.

Effects of Renewal Pattern of Recycled Nutrient Solution on the Ion Balance in Nutrient Solutions and Root Media and the Growth and Ion Uptake of Paprika (Capsicum annuum L.) in Closed Soilless Cultures

Ko, Myat Thaint,Ahn, Tae In,Shin, Jong Hwa,Son, Jung Eek Korean Society of Horticultural Science 2014 원예과학기술지 Vol.32 No.4

Ion imbalance in recycled nutrient solutions is caused by selective ion uptake of plants, which occurs at different rates in different growth stages. The objectives of this study were to investigate the ion balances in both recycled nutrient solutions and rockwool media using different renewal patterns for the nutrient solutions, and to analyze the subsequent effects on uptake of water and nutrients. Over 12 weeks of paprika cultivation, two different renewal patterns (week units) of 6-4-2 and 8-2-2 weeks were compared with a constant renewal pattern of 4-4-4 weeks (control). The nutrient solution in the reservoir tank was constantly maintained at EC $2.5dS{\cdot}m^{-1}$ and pH 5.5-6.5. The changes in the ion balance with the 4-4-4 week pattern were smaller than those with the other treatments. In the early growth stage, however, the ion balances similarly changed among all treatments. Greater changes were subsequently observed for the 6-4-2 week pattern. Although fruit yield and shoot fresh weight of paprika were the lowest with 6-4-2 renewal pattern, no significant differences were observed. Our results indicate that renewal intervals can be extended in consideration of growth stage for more efficient and practical operations in closed soilless cultures.

中翁 李匡贊의 학술사상과 中翁實蹟 연구(1) -『대학』설을 중심으로 -

천병돈 한국양명학회 2023 陽明學 Vol.0 No.70

이광찬은 초기하곡학파라고 할 수 있지만, 그의 철학적 입장은 완전히 주자학을 바탕으로 한다. 주자의 『대학장구』 受容: 이광찬은 주자가 『대학장구』를 통해『대학』사상을 체계화했다는 점에서 매우 높게 평가했다. 격물치지설: ‘格’을 ‘至’로, 物은 事를 포함한 외부 사물을 말한다. ‘致’는 주자학적으로 ‘미루어 극진히 하다(推而極之)’로 이해했다. ‘致’의 중점은 ‘노력과 공부(用力ㆍ工夫)’에 있다. 즉 ‘리’를 인식하는 ‘지극한 지식(極知)’에 이르기 위한 노력이고 공부다. 이러한 ‘知’는 주자학적 ‘知’이다. ‘知’는 인식론적 ‘知’로 이해했다. 따라서 ‘物(事)’은 인식의 대상이지 도덕실천의 대상이 아니다. 주자의 지행병진설 受容: 주자철학에서 도덕실천의 관건은 ‘心’에 있다. 격물궁리와 함양찰식을 통해 心知를 궁극으로 밀고 나가 리와 하나가 되도록 해야 한다. 그러나 주자철학에서 知와 行은 ‘心’ 즉 형이하에서 이루어지는 ‘知와 行’이다. 그러므로 지행병진설을 근거로 지행합일설을 비판한 이광찬의 주장은 타당하지 않다. Kwangchan Lee belongs to the Early Hakgok School. However, his philosophical position is completely based on Zhu Xi’s neo-Confucianism. Accept “Daxuezhangju”: Kwangchan Lee highly praised Zhu Xi for systematizing the idea of “The Great Learning” through “Daxuezhangju”. The inverstgation of things and extension of Knowledge: Inverstgation(格) is ‘to reach(至)’다. Object(物) refers to things(事) that are external to things (things). ‘extension(致)’ understood as Zhu Xi’s ‘expand to the end(推而極之)’. The emphasis of ‘extension’ is on ‘effort and study(用力ㆍ工夫)’. In other words, it is an effort and study to reach ‘ultimate knowledge(極知)’ that recognizes ‘Li(理)’. This ‘knowing’ is the Neo-Confucian ‘knowledge’. Therefore, objects are objects of cognition, not objects of moral practice. Both knowledge and conduct grow: In Zhu Xi’s philosophy, the key to moral practice lies in ‘the mind’. You have to push through what your mind recognizes through The inverstgation of things and extension of Knowledge to the end and become one with Li. However, in Zhu Xi’s philosophy, knowledge and conduct are ‘mind’, that is, ‘knowledge and conduct’ in the phenomenal world. Therefore, Lee Kwang-chan’s argument, which criticized The theory of Unity of knowledge and conduct based on The theory of Both knowledge and conduct grow, is not valid.