Serum Proteomics Analysis of Feline Mammary Carcinoma Based on Label-free and PRM Techniques

Jia-San Zheng,Ren-Yue Wei,Zheng Wang,Ting-Ting Zhu,Hong-Ri Ruan,Xue Wei,Kai-Wen Hou,Rui Wu 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.3

Background: Feline mammary carcinoma is the third most common cancer that affects female cats. Objectives: The purpose of this study was to screen differential serum proteins in feline and clarify the relationship between them and the occurrence of feline mammary carcinoma. Methods: Chinese pastoral cats were used as experimental animals. Six serum samples from cats with mammary carcinoma (group T) and six serum samples from healthy cats (group C) were selected. Differential protein analysis was performed using a Label-free technique, while parallel reaction monitoring (PRM) was performed to verify the screened differential proteins. Results: A total of 82 differential proteins were detected between group T and group C, of which 55 proteins were down regulated and 27 proteins were up regulated. Apolipoprotein A-I, Apolipoprotein A-II (ApoA-II), Apolipoprotein B (ApoB), Apolipoprotein C-III (ApoC-III), coagulation factor V, coagulation factor X, C1q, albumen (ALB) were all associated with the occurrence of feline mammary carcinoma. Differential proteins were involved in a total of 40 signaling pathways, among which the metabolic pathways associated with feline mammary carcinoma were the complement and coagulation cascade and cholesterol metabolism. According to the Label-free results, ApoB, ApoC-III, ApoA-II, FN1, an uncharacterized protein, and ALB were selected for PRM target verification. The results were consistent with the trend of the label-free. Conclusions: This experimen is the first to confirm ApoA-II and ApoB maybe new feline mammary carcinoma biomarkers and to analyze their mechanisms in the development of such carcinoma in feline.

Evidence of multiple reassortment events of feline-to-human rotaviruses based on a rare human G3P[9] rotavirus isolated from a patient with acute gastroenteritis

Nguyen, T.H.,Than, V.T.,Thanh, H.D.,Kim, W. Pergamon Press 2016 Comparative immunology, microbiology and infectiou Vol.46 No.-

<P>A rare human/feline-like rotavirus G3P[9] strain, CAU14-1-262, from a 2-year-old girl with severe gastroenteritis was isolated and sequenced. The 11 gene segments of the CAU14-1-262 strain possessed a novel genotype constellation, G3-P[9]-I3-R3-C3-M3-A3-N3-T1-E3-H6, which was identified for the first time. Phylogenetic analysis of this strain identified the following genome origins: VP7, VP4, VP6, VP1-VP3, NSP1, NSP2, and NSP4 genes possessed an AU-1-like genotype 3 constellation with high sequence identity to those of the feline and human/feline-like rotaviruses; NSP5 possessed a H6 lineage, with highest sequence identity to the human/feline-like E2541 strain; and the NSP3 gene possessed a Wa-like genotype 1 constellation with high sequence identity to those of the of human rotaviruses. These results provided evidence of multiple reassortment events in G3P[9] rotavirus CAU14-1-262 and possibility of feline-to-human interspecies transmission. (C) 2016 Elsevier Ltd. All rights reserved.</P>

Original articles : Comparison of processing times for isolation of feline adipose tissue-derived mesenchymal stem cells

( Hee Ryang Kim ),( Jienny Lee ),( Gyeong Been Lee ),( Jeong Su Byeon ),( Na Yeon Gu ),( In Soo Cho ),( Sang Ho Cha ) 한국예방수의학회(구 한국수의공중보건학회) 2015 예방수의학회지 Vol.39 No.3

Mesenchymal stem cells (MSCs) are an attractive source for cell therapy, as they have the potential for differentiation into multi-lineage cells. Adipose tissue is a safe source due to its easy extraction and abundant resource, with minimal risk to the organ donor. In this study, we attempted to correlate the harvest yield and resulting multipotency of feline adipose tissue-derived mesenchymal stem cells (fAD-MSCs) in accordance with processing time. fAD-MSCs were individually isolated from the abdominal adipose tissues of 6 felines. They were divided into two groups, based on their processing times - Group 1: 0~1 day after adipose tissue harvesting; Group 2: more than 3 days after adipose tissue harvesting. In both groups, the proliferation capacity was analyzed using the cumulative population doubling level (CPDL) calculation assay. The expression levels of MSC-specific markers and differentiation potentials into mesodermal cell lineages were also evaluated. We observed that fAD-MSC isolation yields and CPDL were excellent in Group 1 compared with Group 2. We also found that the differentiation potential-specific genes (ACAN and OPN) were strongly expressed in Group 1 compared with Group 2. These results suggest that for the clinical treatments of feline diseases, fAD-MSCs should be isolated within 1 day after adipose tissue harvesting.

Dynamic analysis of the spinal-pelvic motion match during feline galloping for speed increase into quadruped robotic system

Young Kook Kim,Jongwon Park,Byungho Yoon,Kyung-Soo Kim,Soohyun Kim 제어로봇시스템학회 2014 제어로봇시스템학회 국제학술대회 논문집 Vol.2014 No.10

In terms of the speed performance in feline galloping, relative spinal motion is observed as a key role to increase the locomotion speed. In order to prove this, a domestic cat was observed to investigate galloping gait patterns of the feline. Based on the measurement of the dynamic behavior of a domestic cat, we observed a significant increase in speed through correlated angular rate changes of spinal segments. To support the motion into a quadruped system, the hindlimb stance phase was analyzed using a 2 dimensional planar dynamic model to verify that the external torque generated by spinal motion plays an important role in increasing the ground reaction force in the direction of movement. To verify the analysis, the dynamic simulation demonstrating the hindlimb stance was applied with 3 parameter variations: time, phase and the amplitude of the fitted relative angular velocity profile.

Extensive characterization of feline intra-abdominal adipose-derived mesenchymal stem cells

김희량,이지현,변정수,구나연,이지윤,조인수,차상호 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.3

Mesenchymal stem cells (MSCs) isolated from various tissues have been well characterized for therapeutic application to clinical diseases. However, in contrast to MSCs from other animal species, the characteristics of feline MSCs have not been fully documented. In this study, we conducted extensive characterization of feline adipose tissue-derived MSCs (fAD-MSCs). Study fAD-MSCs were individually isolated from the intra-abdominal adipose tissues of six felines. The expression levels of cell surface markers and pluripotent markers were evaluated. Next, proliferation capacity was analyzed by performing cumulative population doubling level (CPDL) and doubling time (DT) calculation assays. Differentiation potentials of fAD-MSCs into mesodermal cell lineages were analyzed by examining specific staining and molecular markers. All fAD-MSCs positively expressed cell surface markers such as CD29, CD44, CD90, CD105, CD166, and MHC-I, while CD14, CD34, CD45, and CD73 were negatively expressed. The CPDL of the fAD-MSCs was maintained until passage 5 to 6 (P5 to P6), whereas DT increased after P3 to P4. Also, stem cell-specific pluripotent markers (Oct3/4, Nanog, and SSEA-4) were detected. Importantly, all fAD-MSCs demonstrated mesodermal differentiation capacity. These results suggest that fully characterized fAD-MSCs could be beneficial when considering the use of these cells in feline disease research.

Incidence and sero-surveillance of feline viruses in Korean cats residing in Gyeonggi-do

Yang, Dong-Kun,Park, Yu-Ri,Kim, Eun-ju,Lee, Hye Jeong,Shin, Kyu-Sik,Kim, Ju-Hun,Lee, Kyunghyun,Hyun, Bang-Hun The Korean Society of Veterinary Science 2022 大韓獸醫學會誌 Vol.62 No.3

Incidences of major feline viral diseases provide basic information for preventing viral disease in cats. Despite the growing interest in feline viral diseases, sero-surveillances have been lacking. In this study, we analyzed the diagnoses of feline viral diseases and conducted a sero surveillance of feline panleukopenia virus (FPV), feline calicivirus (FCV), feline herpesvirus-1 (FHV-1), and feline infectious peritonitis virus (FIPV) in Korean cats. Of the 204 confirmed cases since 2015, the numbers of diagnoses for FPV, FIPV, FCV, feline influenza virus, and FHV-1 were 156, 32, 12, 3, and 1 case, respectively. In total, 200 sera, collected between 2019 and 2021, were screened for the presence of antibodies against FPV, 2 FCVs, FHV-1, and FIPV using a hemagglutination inhibition test and a virus-neutralizing assay (VNA). The overall seropositive rates in cats tested for FPV, the 2 FCVs, FHV-1, and FIPV were 92.5%. 42.0%, 37.0%, 52.0%, and 14.0%, respectively. A low correlation (r = 0.466) was detected between the VNA titers of 2 FCV strains. The highest incidence and seropositive rate of FPV reveal that FPV is circulating in Korean cats. The low r-value between 2 FCVs suggests that a new feline vaccine containing the 2 kinds of FCVs is required.

Development and evaluation of a triplex real-time quantitative reverse transcription-polymerase chain reaction for rapid and differential detection of three feline respiratory viral pathogens

( Ji-su Baek ),( Jong-min Kim ),( Hye-ryung Kim ),( Ji-hoon Park ),( Yeun-kyung Shin ),( Hae-eun Kang ),( Jung-hoon Kwon ),( Won-jae Lee ),( Min Jang ),( Sang-kwon Lee ),( Ho-seong Cho ),( Yeonsu Oh ) 한국동물위생학회 2023 韓國家畜衛生學會誌 Vol.46 No.4

In this study, a new triplex real-time quantitative reverse transcription polymerase chain reaction (tqRT-PCR) assay was developed for the rapid and differential detection of three feline viral pathogens including feline calicivirus (FCV), feline herpesvirus 1 (FHV-1), and influenza A virus (IAV) in a single reaction. The assay specifically amplified three targeted viral genes with a detection limit of below 10 copies/reaction. The assay showed high repeatability and reproducibility, with intra- and inter-assay coefficients of variation of less than 1%. Based on the diagnostic results of the assay using 120 clinical samples obtained from cats with feline respiratory disease complex (FRDC)-suspected signs, the prevalence of FCV, FHV-1, or IAV was 43.3%, 22.5%, or 0%, respectively, indicating that the diagnostic sensitivity was comparable or superior to those of previously reported monoplex qRT-PCR/qPCR assays. The dual infection rate for FCV and FHV-1 was 8.3%. These results indicate that FCV and FHV-1 are widespread and that co-infection with FCV and FHV-1 frequently occur in the Korean cat population. The developed tqRT-PCR assay will serve as a promising tool for etiological and epidemiological studies of these three bacterial pathogens, and the prevalence data for three feline viruses obtained in this study will contribute to expanding knowledge about the epidemiology of FRDC in the current Korean cat population.

Prevalence of feline viral agents in South Korea from 2020 to 2022

Ilseob Lee,Sung-Hee Kim,Kyoung-Ki Lee,Bok-Kyung Ku,Hye-Young Jeoung 한국예방수의학회 2023 한국예방수의학회 학술대회자료집 Vol.2023 No.-

Immunogenicity of a new inactivated vaccine against feline panleukopenia virus, calicivirus, and herpesvirus-1 for cats

Dong-Kun Yang,Yu-Ri Park,Eun-Ju Kim,Hye Jeong Lee,Subin Oh,Bang-Hun Hyun 대한수의학회 2023 大韓獸醫學會誌 Vol.63 No.1

Feline panleukopenia virus (FPV), feline calicivirus (FCV), and feline herpesvirus type-1 (FHV-1) are major infectious pathogens in cats. We evaluated the immunogenicity of a new vaccine containing inactivated FPV, two FCVs, and FHV-1 in animals. An FPV, two FCVs, and an FHV-1 isolate were continuously passaged 70, 50, 80, and 100 times in CRFK cells. FP70, FC50, FC80, and FH100 were propagated and used as vaccine antigens. Two inactivated feline virus vaccines, feline rehydragel-adjuvanted vaccine (FRAV) and feline cabopol-adjuvanted vaccine (FCAV) were prepared and inoculated into mice and guinea pigs. Humoral immune responses were measured using hemagglutination inhibition (HI) for FPV and virus-neutralizing antibody (VNA) for two FCVs and FHV-1 tests. Serial passages in CRFK cells resulted in increase in titers of FPV and two FCVs but not FHV-1 The FCAV induced higher mean HI and VNA titers than the FRAV in guinea pigs; therefore, the FCAV was selected. Cats inoculated with FCAV developed a mean HI titer of 259.9 against FPV, and VNA titers of 64, 256, and 3.2 against FCV17D03, FCV17D283, and FHV191071, respectively. Therefore, cats inoculated with the FCAV showed a considerable immune response after receiving a booster vaccination.

Exosomes derived from feline adipose tissue mesenchymal stem cells reduce inflammation factors

Soo-Eun Sung,Minkyoung Sung,Kyung-Ku Kang,Joo-Hee Choi,Sijoon Lee,KilSoo Kim,Min-Soo Seo 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Feline adipose tissue derived mesenchymal stem cells (fAD-MSCs) release extracellular vesicles like exosome, apoptotic bodies and microparticles. Particularly, exosomes are formed inward a cell, throughout multivesicular bodies (MVB), so it contains genetic materials such as protein, DNA, and RNA are similar to the parent cells. The exosomes research is rapidly expanding, with increase publication in recent years so the function and application of MSC-derived exosomes should emerge as cell-free therapeutics. Exosomes were isolated from feline AD-MSCs and feline fibroblasts cell culture medium using ultracentrifugation. Characterization of feline exosomes by FACS, NTA and TEM imaging. And then, cytokines levels were detected by sandwich ELISA procedure in exosomes and LPS-induced THP-1 macrophages. The size of isolated exosomes had typical exosome size approximately 150 nm and the exosome expressed tetraspanin CD9 and CD81. Anti-inflammatory factor IL-10 was increased in feline ADMSCs derived exosomes. However, the pro-inflammatory factors such as IL-1β, IL-8, IL- 2, RANTES and IFN-gamma were significantly decreased in feline AD-MSC exosomes. This results were related that inhibition of inflammation response in LPS-induced THP-1 macrophage through expressed low levels of cytokines such as TNF-α, IL-1 β. It was the first demonstrated that feline AD-MSCs derived exosomes enhanced the inflammatory suppressive effects and it had the therapeutic potential of immune disease or inflammation inhibition therapy.