소리쟁이(Rumex crispus) 뿌리로부터 가용성 고형분의 추출특성

정갑섭 ( Kap Seop Jeong ) 한국환경과학회 2011 한국환경과학회지 Vol.20 No.10

In this study, the extraction characteristics of soluble solid from Rumex crispus(Curled dock) was studied from the investigation of the effects of experimental conditions on extraction rate; extraction ratio, composition of extractants, extraction time and pH of extractant, etc. The proximate composition of Rumex crispus was 2.58% crude lipid, 5.59% crude protein, 7.39% crude ash, 6.13% moisture and 78.31% carbohydrate, respectively. Turbidity of extract by distilled water was higher and increased with extraction time and extraction temperature, where as the turbidity didn`t increase by ethanol and methanol in 20 folds of extraction ratio. Turbidity was inversely proportional to the extraction ratio for the three extractants at 25℃ and 1 hour extraction. But turbidity of extract was highest by composition of 50% methanol-water extractant than any other compositions of extractants. Eighteen and fifteen free aminoacids were detected in extracts with distilled water, methanol and ethanol extractant, respectively, and it`s contents were order of glutamic acid>proline>aminobutyric acid>alanine. The extraction rate of soluble solid from Rumex crispus was order of distilled water>methanol>ethanol within experimental extraction ratio. In extraction with distilled water, the contents of soluble solid was inversely proportional to the pH of extractant.

Extraction of mangiferin from Mahkota Dewa (Phaleria macrocarpa) using subcritical water

Wan-Joo Kim,김재덕,Bambang Veriansyah,Youn-Woo Lee,김재훈 한국공업화학회 2010 Journal of Industrial and Engineering Chemistry Vol.16 No.3

A pharmacological active component, mangiferin, was extracted from Mahkota Dewa using subcritical water extraction. The subcritical water extractions were carried out at temperatures ranging 323–423 K,pressures ranging 0.7–4.0 MPa, and extraction times ranging 1–7 h. Extraction yield of mangiferin was measured using high-performance liquid chromatography (HPLC). The extraction yield was strongly dependent on the temperature while weakly dependent on the extraction pressure. As the extraction temperature increased, the extraction mangiferin yield increased, possibly resulting from the decrease in polarity of subcritical water at higher temperature. At an optimal extraction condition of 373 K, 4.0 MPa and extraction time of 5 h, the extraction yield of mangiferin was 21.7 mg/g. This value was close to the extraction yield with methanol (25.0 mg/g) and higher than those with water (18.6 mg/g) or ethanol (13.2 mg/g) at their boiling points. 2010 Published by Elsevier B.V. on behalf of The Korean Society of Industrial and Engineering Chemistry.

탈지미세조류로부터 초음파추출을 이용한 항산화 물질 생산 공정 최적화

조재민 ( Jaemin Jo ),신슬기 ( Suelgl Shin ),정현진 ( Hyunjin Jung ),민보라 ( Bora Min ),김승기 ( Seungki Kim ),김진우 ( Jinwoo Kim ) 한국화학공학회 2017 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.55 No.4

초음파 추출법(Ultrasonic-assisted extraction, UAE)은 기존의 추출법 대비 높은 추출 효율과 짧은 추출시간으로 식물 세포벽으로부터 생리활성물질 추출 또는 분리에 효과적인 방법으로 인식되어 관심이 증대되고 있다. 본 연구에서는 탈지미세조류(Tetraselmis KCTC 12236BP)에서 폴리페놀 추출을 위해 UAE를 적용하여 총 폴리페놀(TPC) 생산에 미치는 주요 추출변수의 영향을 평가하였다. 추출변수의 최적화를 위해 입자크기, 고액비(L/S ratio), 에탄올 농도, 추출 온도 및 추출 시간을 요인으로 하여 순차적인 최적화를 진행하였다. 실험에 적용 된 모든 변수는 TPC 추출에 유의한 효과를 보였으며 추출 온도가 TPC생산에 가장 큰 영향을 미침을 확인 할 수 있었다. 최적 추출조건은 혼합 입자 사용 시, 10%고액비, 60% 에탄올, 추출온도 100℃와 추출시간 30 분을 적용한 추출에서 8.7 mg GAE/g DW를 얻을 수 있었다. 동일한 추출조건에서 열수추출과 UAE를 비교하였을 때 UAE에서 TPC 추출이 1.8배 증가함을 확인하였다. 본 연구를 통해 저온 및 짧은 추출시간을 적용한UAE가 기존의 열수추출 공정에 비해 LEA를 이용한 생리활성물질 생산에 보다 효과적임을 확인하였다. Ultrasound-assisted extraction (UAE) has attracted growing interest, as it is an effective method for the rapid extraction of bioactive compounds from plants with a high extraction efficiency comparable to the conventional extraction. In this study, UAE was used for the extraction of polyphenols from lipid extracted microalgae (Tetraselmis KCTC 12236BP) and the effects of five extraction variables on the total phenolic compounds (TPC) were studied. For the optimization of extraction parameters, particle size, solid-to-liquid (L/S) ratio, ethanol concentration, extraction temperature and extraction time have been examined as independent variables. All variables exhibited the significant effects on the extraction of TPC and extraction temperature showed the most significant effect among five variables. The optimal extraction conditions were the extraction using mixed particle, S/L ratio of 10%, ethanol concentration of 60%, extraction temperature of 100℃ and extraction time of 30 min, which gave the 8.7 mg GAE/g DW for TPC. Compared with conventional hot-water extraction, TPC extraction under UAE was increased by up to 1.8 fold with same extraction condition. This study showed that UAE under low temperature and short extraction time was proven to be an effective extraction process for TPC production from LEA compared to conventional hot-water extraction process.

추출 방법과 조건에 따른 소나무 지엽 추출효율 변화

김동성(Dong Sung Kim),김형민(Hyung Min Kim),성용주(Yong Joo Sung),강석구(Seog Goo Kang),강호양(Ho-Yang Kang),이준우(Jun-Woo Lee),김세빈(Se Bin Kim),박관수(Gwan-Soo Park) 한국펄프·종이공학회 2016 펄프.종이技術 Vol.48 No.1

The extraction efficiency depending on the extracting methods and the conditions of extraction was investigated. The common steam extraction was compared to the distillation extraction method. The effects of the samples size and the extraction time on the extract yield were also investigated by using UV-Vis spectrophotometer. One of the functional components of pine needle extract as the natural phenol base components were detected by the UV-VIS at around 235 nm wavelength range. The absorbance intensity at around 235 nm wavelength of the pine needle extract was used as the indicator of the extraction efficiency in this experiment. The distillation extraction showed the higher extract yield than the steam extraction. The grinding treatment of pine needles also resulted in the better extract performance, but the severe grinding showed a little decrease in the extract yield especially in case of the distillation extraction method. More than half of the extract was collected at the first stage of the extraction, that was the first 15 minutes in the total 60 minutes extraction.

금은화의 폴리페놀, 플라보노이드, Chlorogenic acid의 최적 추출조건

송선영,김명기,하헌용 조선대학교 공학기술연구원 2018 공학기술논문지 Vol.11 No.1

This study was carried out to compare of extraction efficiency for Lonicera Flos by extraction conditions. To compare the extraction efficiency of active ingredients by the extraction methods and ratio of water to ethanol (0, 20, 40, 60, 80 and 100% water) for Lonicera japonica Flos was extracted by ultrasonic extraction, shaking extraction water-bath extraction, and soaking extraction. Contents of phenolic compounds were the highest with 127.9 mg GAE/g DW in 60% ethanol by hot-water extraction method at 80℃ and contents of flavonoids were highest with 29.8 mg GAE/g DW in 80% ethanol by hot-water extraction method at 80℃. Contents of chlologenic acid were the highest with 31.8 mg/g in 80% ethanol by hot-water extraction method at 80℃. Extraction of the bioactive ingredient of Lonicera japonica Flos was effective in 60-80% ethanol by hot-water extraction.

탈지미세조류로부터 단백질 고함량화를 위한 알칼리 추출공정 최적화

신슬기(Seulgi Sin),조재민(Jaemin Jo),정현진(Hyunjin Jung),서주현(Joo-Hyun Seo),김진우(JinWoo Kim) 한국생물공학회 2017 KSBB Journal Vol.32 No.4

In this study, an attempt was made to optimize protein extraction from lipid extracted microalgae (LEA) by alkali solution. To enhance the productivity, extraction solvent, extraction pH, temperature, time and precipitation pH were evaluated to maximize the protein extraction from defatted Tetraselmis KCTC 12236BP. Independent variables tested were organic solvents, extraction pH (5-13), precipitation pH (2.3-4.0), extraction time (2.6-9.4 h) and extraction temperature (26-94°C). Alkali solution (2 mol NaOH) was found to be effective in extraction evaluating, the impact of the solvents on protein extraction. The results showed that the extraction pH was an influencing variable and extraction yield increased both with increase in extraction temperature time and precipitation pH. The yield of protein showed a maximum level at temperature of 64°C and precipitation pH of 3.38, respectively. Under the optimal condition, LEA protein yield reached 71.7% as compared to 34.4% with the initial condition with 50°C, 30 min of agitation and 1 h of precipitation, representing a 2.1 fold increase in protein yield.

Haematococcus pluvialis로부터 아스타잔틴 추출을 위한 추출공정 조건 최적화

김송이,전성진,김호섭,감다혜,김진우 한국생물공학회 2019 KSBB Journal Vol.34 No.4

In this study, the selection of extraction solvent and optimization of extraction condition were carried out to increase the extraction of astaxanthin, an antioxidant material produced from Haematococcus pluvialis. For increasing the extraction of astaxanthin through more effective cell disruption, the concentration of acetone, rotation speed of homogenizer, extraction temperature, and extraction time were sequentially optimized. When acetone (50% v/v) was used as an extract solvent, the extraction concentration of astaxanthin was found to be the highest (1.41 μg/mL), and the extraction concentration increased 3.3 times compared to ethanol extraction. As the concentration of acetone increased to 99.5% (v/v), it was found that the concentration of astaxanthin increased proportionally to 2.47 μg/mL. When evaluating the effect of rotation speed and extraction temperature, astaxanthin concentration was maximized to 3.93 g/mL at 15,318 rpm (impeller tip speed = 5.67 m/s) and 52.5oC. In addition, as extraction time increased, the concentration of extract was increased and resulted in a maximum of 4.76 μg/mL at 16.6 min which was a 10.9-fold increase compared to ethanol extraction indicating that acetone is more effective than conventional solvents in the extraction of astaxanthin.

추출조건이 노니 부정근에 함유된 rubiadin의 추출 효율에 미치는 영향

김명기(Myong-Ki Kim),정철승(Cheol-Seung Jeong),신용국(Yong-Kook Shin),박경희(Kyong-Hee Park),이운장(Woon-Jang Lee),이은정(Eun-Jung Lee),백기엽(Kee-Yoeup Paek) 한국원예학회 2010 원예과학기술지 Vol.28 No.4

노니(Morinda citrifolia) 부정근의 주요 성분인 rubiadin은 간 보호 효과가 있어 제약산업에서 높은 가치가 있다. 노니 부정근의 주요 유효성분인 rubiadin의 효율적인 추출조건을 규명하고자 용매 종류, 물과 메탄올의 비율(물, 20%, 40%, 60%, 80%, 100%), 추출시간 및 추출방법을 달리하여 추출하였다. 노니 부정근에서의 rubiadin의 함량분석은 HPLC이용 분석조건을 확립하였으며, C-18 컬럼을 사용하여 280nm에서 메탄올과 물로 농도구배를 주어 분석하였다. 용매별추출효율은 메탄올(0.08%)>에탄올(0.05%)>아세토니트릴(0.03%)>아세톤(0.02%) 및 메틸렌클로라이드(0.02%)순으로 증가하였다. 메탄올에 물의 혼합 비율을 달리하여 초음파 추출기로 1시간 동안 추출한 결과 60% 메탄올(0.21%)>80% 메탄올(0.13%)>100% 메탄올(0.07%) 및 40% 메탄올(0.07%) 순으로 효과적이었으며, 환류냉각 추출기로 2시간 동안 추출한 결과 60% 메탄올(0.21%)>40% 메탄올(0.17%)>80% 메탄올(0.14%) 용매처리구 순으로 효율이 좋았다. 추출방법 및 추출시간에 따른 rubiadin의 추출 효율을 비교하기 위해 환류냉각추출 및 초음파추출, 진탕추출법으로 추출하였다. 추출방법 및 추출시간에 따른 rubiadin의 추출효율은 초음파추출 및 진탕추출에서 각각 8 및 24시간동안 추출했을 때 효율적이었다. Rubiadin, a major compound of noni (Morinda citrifolia) adventitious root, is highly valued in pharmaceutical industry due to hepatoprotective activity. To dissect rubiadin’s effective extraction condition, extraction process of noni adventitious roots was performed with different solvent types, ratio of water to methanol (water, 20, 40, 60, 80, and 100% of methanol), extraction time, and extraction method. In contrast, we also developed a reverse- phase HPLC assay method to determine rubiadin from noni adventitious roots. The HPLC assay of rubiadin was performed by C-18 column using a gradient solvent system of methanol and water with UV detector at 280 ㎚. The extraction efficiency of different types of solvents were increased in order of methanol (0.08%)>ethanol (0.05%)>acetonitrile (0.03%)>acetone (0.02%) and methylene chloride (0.02%). The results of rubiadin extraction using different solvents showed that 1 hour of ultrasonic extraction was effective in order of 60% methanol (0.21%)>80% methanol (0.13%)>100% methanol (0.07%), 40% methanol (0.07%) and 2 hours of reflux extraction was effective in order of 60% methanol (0.21%)>40% methanol (0.17%)>80% methanol (0.14%). To compare the extraction efficiency of rubiadin according to the extraction methods and time for high rubiadin content, the extracts of rubiadin in noni adventitious roots were isolated with the methods of ultrasonic extraction, shaking extraction and reflux extraction. Rubiadin extracted from the methods of ultrasonic waves and shaking displayed the highest contents at 8 and 24 hours, respectively.

Effect of warfarin discontinuation on the incidence of postoperative bleeding in tooth extraction

Jung-Soo Lee,Moon-Key Kim,Sang-Hoon Kang 대한구강악안면외과학회 2020 대한구강악안면외과학회지 Vol.46 No.4

Objectives: The number of patients undergoing oral anticoagulant therapy for cardiovascular and cerebrovascular disease is increasing. However, the risk of bleeding after tooth extraction in patients receiving warfarin is unclear. Here, we assess the risk of bleeding after tooth extraction in patients on warfarin. Materials and Methods: The study included 260 patients taking warfarin who underwent tooth extraction (694 teeth). The patients were divided into those whose teeth were extracted while they were taking warfarin, those who discontinued warfarin before extraction, and those who underwent extraction while receiving heparin bridging therapy. Bleeding complications in the two groups were compared. Results: Of the 260 patients, 156 underwent extraction while taking warfarin, 70 stopped taking warfarin before extractions, and 34 received heparin bridging therapy and stopped taking either medication before extractions. Bleeding complications occurred in 9 patients (3.5%) and 9 tooth sites (1.3%). Among the 9 patients with bleeding complications, 6 underwent extraction while taking warfarin, 2 stopped warfarin before extraction, and 1 underwent extraction after receiving heparin bridging therapy. No significant difference was seen between patient groups regarding bleeding after extractions (P=0.917). Conclusion: Warfarin use does not increase the risk of post-extraction bleeding and can therefore be continued during tooth extraction.

Solvent Microextraction of Methamphetamine and its Metabolite, Amphetamine, in Urine with Simultaneous Back-Extraction

Sihn, Young-Sihn,Baeck, Seung-Kyung,Kim, Sun-Chun,Park, Yoon Shin,Chung, Hee-Sun 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-