옥수수 수염의 이화학적 특성과 변이

김선림,박철호,김이훈,허한순,손영구 韓國作物學會 2000 한국작물학회지 Vol.45 No.6

옥수수 수염의 일반적인 생육특성과 함유된 각종 일반성분 및 유리당, 유기산, 아미노산 및 향기성분에 관한 기초자료를 제공하기 위하여 본 시험을 수행하였으며 얻어진 결과를 요약하면 다음과 같다. 1. 옥수수의 수염은 출사 후 3일 이내에 수정이 완료되고 길이 신장이 정지되나 수정되지 않은 수염은 지속적으로 신장하여 출사 후 8일에 23-25cm였다. 2. 출사기에 옥수수 수염의 수분함량은 92-94%, 출사후 30일에는 70-75%였다. 3.옥수수 수염은 엽록소 b의 함량이 높고, 엽록소 a/b의 비율은 사료용 옥수수> 단옥수수> 초당옥수수> 찰옥수수 순이었다. 4. 유리당은 glucose와 fructose가 주종을 이루고, 그 구성비율은 glucose가 55% fructose가 42% 이었으며 sucrose 및 maltose는 4% 미만으로 낮았다. 5. 가용성 고형물 함량은 미수정 수염이 13.7-16.8 Brix %, 수정된 수염은 12.6-13.7 Brix %로 1.1-3.1 Brix % 차이가 있었다. 6. 옥수수 수염의 유기산은 phytic, oxalic, malic, shikimic, glutaric 및 acetic acid가 검출되었는데, 이중 phytic, oxalic 및 glutaric acid가 주요 유기산이었고 유기산의 함량은 사료용 옥수수> 초당옥수수> 찰옥수수> 단옥수수 순으로 높았다. 7. 단백질 함량은 3.36-4.17%로서 출사후 3일에 최대치를 나타내었고, 단백질 함량은 사료용 옥수수> 찰옥수수> 단옥수수> 초당옥수수 순으로 높았다. 8. 아미노산중 serine, glycine 및 threonine 3종은 10% 이상 함유되어있었으며 glycine의 구성비율이 12.3%로 가장 높았고 황(S)함유 아미노산인 methionine과 cystine 함량은 각각 2.1% 및 1.3%로 함량이 낮았다. 9. 향기성분으로 acetaldehyde, ethanol, acetone, DMS, isobutylaldehyde, cis-3-hexanol, 3-hexe-1-ol, acetate, trans-2-hexanol, pentanol 등이 검출되었고, 사료용의 주요 향기성분은 acetaldehyde와 DMS, 찰옥수수는 acetaldehyde, ethanol 및 DMS였다. 10. 옥수수 수염의 길이는 유기산 함량과 r=0.556, 아미노산 함량과는 r=-0.514로 유의성이 인정되었고, 유리당 함량은 유기산 함량과 r=0.703, flavonoid 함량과 r=0.544로 유의한 정의상관이 있었다. 특히, flavonoid 함량은 엽록소 함량과 정의 상관(r=0.523)이 인정되어 엽록소는 flavonoid 함량의 판단지표로 활용될 수 있을 것으로 사료되었다. This study was carried out to investigate the physicochemical characteristics of corn silks. Pollination of corn silks was finished within three days after silking, and elongation of open pollinated corn silks was stopped, but unpollinated silks elongated until eight days after silking. Moisture contents of corn silks were about 92-94% at silking stage, but continuously decreased. these were about 70-75% at 30 days after silking. Chlorophyll b was higher than chlorophyll a in corn silks, and chlorophyll a/b ratios of four hybrids were high in this order : silage > sweet > super sweet > waxy corn. Free sugars in corn silks were mainly fructose, glucose and maltose. Their composition rate was 55% of glucose, 42% of fructose and 4% of maltose. Water soluble solid (Brix %) contents of unpollinated corn silks were ranged from 13.7 to 16.8 Brix % and pollinated corn silks were from 12.6 to 13.7 Brix %. Phytic, oxalic, malic. shikimic, glutaric and acetic acid were detected on corn silks. Phytic, oxalic and glutaric acid were considered as a major organic acids in corn silks. Amino acids in corn silks were high in this order : waxy > silage > sweet >sweet corn. Serine, glycine and thereoine were contained more than 10%, and five amino acids such as aspartic, glutaric, arginine, alanine and proline were ranged about 5 to 8%. Methionine and cystine, amino acids containing sulfur were contained only small quantity as about 2.1% and 1.3%, respectively. Acetaldehyde, ethanol, acetone, DMS, isobutylaldehyde, cis-3-hexanol, 3-hexe-1-ol, acetate, trans-2-hexanol and pentanol were detected as the volatile components in corn silks, but acetaldehyde and DMS were major volitiles in silage corn silks, and acetaldehyde, ethanol and DMS were major volitiles in waxy corn silks. The length of corn silks was a positively correlated with organic acids (r=0.556* ), and a negatively correlated with amino acids (r=-0.514* ), respectively. Free sugars were positively correlated with all characteristics tested and significantly correlated with organic acid (r=TEX>0.703**), and flavonoids (r=TEX>0.544*). Chlorophyll was significantly correlated with flavonoid contents (r=TEX>0.523*). For this reason chlorophyll was evaluated as an indirect selection character for the high flavonoid containing varieties.

옥수수수염에 함유된 Flavonoids의 분리 및 동정

김선림,김미정,이유영,정건호,손범영,이진석,권영업,박용일 한국작물학회 2014 한국작물학회지 Vol.59 No.4

This study was carried out to isolate and characterizethe flavonoids present in corn silks. Maysin content in theunpollinated corn silks (Kwangpyeongok) showed its highestlevel at 3 days after silking, and decreased thereafter, whilethe content of open pollinated silks were consistently decreasedafter silking. This result indicates that the maysin contentis considerably affected by the pollination of corn silk. Unpollinated corn silks were collected with excising, andethanol employed to extract flavonoids at common temperaturefor 9 days. After extraction, chlorophyll, lipids etc. wereremoved with methylene chloride, then submitted to flashcolumn cartridge (150 × 40 mm i.d.) packed with a preparativeRP-C18 bulk packing material (125 Å, 55-105 μm), andmonitored at 352 nm. Four fractions, fraction-I, -II, -III,and -IV, were isolated from ethanolic extract of corn silks. Absorption spectrum of fraction I showed its maximum intensity(λmax) at 327 nm and 239 nm, fraction-II showed its maximumintensity at 339 nm and 274 nm, fraction-III showed itsmaximum intensity at 345 nm and 277 nm, and fraction-IVshowed its maximum intensity at 352 nm, 270 nm, 257 nm,respectively. On the baisis of ESI micro-TOF analysis, fraction-Iwas identified as chlorogenic acid (m/z 355, 3-(3,4-dihydroxycinnamoyl)quinic acid, C16H18O9), fraction-II identifiedas a mixture of chlorogenic acid and luteolin 3'-methylether 7-glucuronosyl-(1→2)-glucuronide (m/z 653, C28H28O18),fraction-III identified as a mixture of chlorogenic acid luteolin7-O-neohesperidoside (m/z 595, C27H30O15), and luteolin 3'-methylether 7-glucuronosyl-(1→2)-glucuronide, and fraction-IV identifiedas maysin (m/z 577, 2''-O-α-L-rhamnosyl-6-C-(6-deoxy-xylohexose-4-ulosyl)luteolin, C27H28O14), respectively. From theethanolic extract of corn silks, fraction-I was obtained about35 mg/100 g F.W., fraction-II was about 48 mg/100 g F.W.,fraction-III was about 46 mg/100 g F.W., and fraction-IVwas about 138 mg/100 g F.W., respectively

Effects of Delayed Pollination on Kernel Development in Corn

Myoung Hoon Lee 韓國作物學會 1998 Korean journal of crop science Vol.43 No.1

Receptivity of corn silk to pollen decreases with time. For effective pollination, it is important to study the receptivity of corn silks in relation to time elapsed after their emergence. Two commercial corn hybrids, 'Kwanganog' and 'Suwon 19', were tested for their silk growth and effects of delayed pollination on kernel development for 1 to 13 days after emergence of the first silk. Silks elongated rapidly for the first 3 days and then gradually decreased. Silks grew more than 30mm per day for the first 3 days and stopped growing on the 11 or 12th day after emergence of the first silk. Filled ear length decreased slowly for the first 8 days, and then decreased rapidly. Similar trends were observed for number of kernel rows and number of kernels per row or ear. The highest numbers of kernels per row or ear were observed when plants were pollinated 2-4 days after silking. These numbers decreased gradually up to 8 days, and then decreased sharply. This result indicates that 8 day-delayed pollination has no influence on kernel development. There were negative correlations between silk length and ear characteristics except kernel weight. Silk growth rate was positively correlated with filled ear length, row number, and kernel number. It might be assumed that delayed pollination by one week after the first silk emerged would not affect on kernel set

Effects of Delayed Pollination on Kernel Development in Corn

Lee, Myoung-Hoon The Korean Society of Crop Science 1998 Korean journal of crop science Vol.43 No.1

Receptivity of corn silk to pollen decreases with time. For effective pollination, it is important to study the receptivity of corn silks in relation to time elapsed after their emergence. Two commercial corn hybrids, 'Kwanganog' and 'Suwon 19', were tested for their silk growth and effects of delayed pollination on kernel development for 1 to 13 days after emergence of the first silk. Silks elongated rapidly for the first 3 days and then gradually decreased. Silks grew more than 30mm per day for the first 3 days and stopped growing on the 11 or 12th day after emergence of the first silk. Filled ear length decreased slowly for the first 8 days, and then decreased rapidly. Similar trends were observed for number of kernel rows and number of kernels per row or ear. The highest numbers of kernels per row or ear were observed when plants were pollinated 2-4 days after silking. These numbers decreased gradually up to 8 days, and then decreased sharply. This result indicates that 8 day-delayed pollination has no influence on kernel development. There were negative correlations between silk length and ear characteristics except kernel weight. Silk growth rate was positively correlated with filled ear length, row number, and kernel number. It might be assumed that delayed pollination by one week after the first silk emerged would not affect on kernel set.

옥수수수염에 함유된 Flavonoids의 분리 및 동정

김선림,김미정,이유영,정건호,손범영,이진석,권영업,박용일 한국작물학회 2014 한국작물학회지 Vol.59 No.4

본 연구는 옥수수수염에 함유된 flavonoid 계열의 물질인 maysin, luteolin 7-O-neohesperidoside, luteolin 3'-methyl ether 7-glucuronosyl-(1→2)-glucuronide 및 polyphenol성 물 질인 chlorogenic acid을 추출 및 분리하는 방법에 관한 것 으로, 얻어진 결과를 요약하면 다음과 같다. 1. 수정되지 않은 옥수수수염의 메이신 함량은 출사 후 3 일에 함량이 최대치에 도달하고, 그 후 감소되는 것으 로 나타났으며, 방임수분된 옥수수수염의 메이신 함량 은 출사후 지속적으로 감소되는 것으로 나타나 화분의 수분 여부에 따라 메이신 함량에 많은 차이가 있음을 알 수 있었다. 2. 채취한 옥수수수염에 에탄올을 가하여 9일간 상온에 서 추출 후 엽록소, 지질 및 당질 등을 제거시키고, C18 column chromatography를 수행하여 분취물 I, II, IIIIV를 얻었다. 3. 분취물의 흡광도를 분석한 결과 분취물 I은 327 nm 및 239 nm에서 최대 흡수 파장(λmax)을 나타내었고, 분취물 II는 339 nm 및 274 nm, 분취물 III는 345 nm 및 277 nm, 분취물 IV는 352 nm, 270 nm, 및 257 nm 에서 최대 흡수파장을 나타내었다. 4. 분취물 I은 m/z 355[M+H]+인 chlorogenic acid(3-(3,4- dihydroxycinnamoyl)quinic acid, C16H18O9)이었으며, 분취물 II는 chlorogenic acid와 m/z 653[M+H]+인 luteolin 3'-methyl ether 7-glucuronosyl-(1→2)-glucuronide (C28H28O18) 를 함유한 혼합물, 분취물 III는 chlorogenic acid와 m/z 595[M+H]+인 luteolin 7-O-neohesperidoside (C27H30O15) 및 luteolin 3'-methyl ether 7-glucuronosyl-(1→2)-glucuronide를 함유한 혼합물이었고, 분취물 IV는 m/z 577[M+H]+인 maysin (C27H28O14, 2''-O-α-L-rhamnosyl- 6-C-(6-deoxy-xylo-hexose-4-ulosyl)luteolin 임을 각각 확인 하였다. 5. 옥수수수염의 알코올 추출물로부터 분리된 분취물 I은 옥수수수염 생체 100 g 당 (수분함량 약 92%) 35 mg/ 100 g, 분취물 II는 48 mg/100 g, 분취물 III는 46 mg/ 100 g, 분취물 IV는 138 mg/100 g을 얻을 수 있었다. This study was carried out to isolate and characterize the flavonoids present in corn silks. Maysin content in the unpollinated corn silks (Kwangpyeongok) showed its highest level at 3 days after silking, and decreased thereafter, while the content of open pollinated silks were consistently decreased after silking. This result indicates that the maysin content is considerably affected by the pollination of corn silk. Unpollinated corn silks were collected with excising, and ethanol employed to extract flavonoids at common temperature for 9 days. After extraction, chlorophyll, lipids etc. were removed with methylene chloride, then submitted to flash column cartridge (150 × 40 mm i.d.) packed with a preparative RP-C18 bulk packing material (125 Å, 55-105 μm), and monitored at 352 nm. Four fractions, fraction-I, -II, -III, and -IV, were isolated from ethanolic extract of corn silks. Absorption spectrum of fraction I showed its maximum intensity (λmax) at 327 nm and 239 nm, fraction-II showed its maximum intensity at 339 nm and 274 nm, fraction-III showed its maximum intensity at 345 nm and 277 nm, and fraction-IV showed its maximum intensity at 352 nm, 270 nm, 257 nm, respectively. On the baisis of ESI micro-TOF analysis, fraction-I was identified as chlorogenic acid (m/z 355, 3-(3,4-dihydroxycinnamoyl) quinic acid, C16H18O9), fraction-II identified as a mixture of chlorogenic acid and luteolin 3'-methyl ether 7-glucuronosyl-(1→2)-glucuronide (m/z 653, C28H28O18), fraction-III identified as a mixture of chlorogenic acid luteolin 7-O-neohesperidoside (m/z 595, C27H30O15), and luteolin 3'-methyl ether 7-glucuronosyl-(1→2)-glucuronide, and fraction-IV identified as maysin (m/z 577, 2''-O-α-L-rhamnosyl-6-C-(6-deoxy-xylohexose- 4-ulosyl)luteolin, C27H28O14), respectively. From the ethanolic extract of corn silks, fraction-I was obtained about 35 mg/100 g F.W., fraction-II was about 48 mg/100 g F.W., fraction-III was about 46 mg/100 g F.W., and fraction-IV was about 138 mg/100 g F.W., respectively.

옥수수수염 추출물이 SW480 Colon Cancer Cell에서 NF-κB와 염증성 사이토카인 발현에 미치는 영향

최현지 ( Hyunji Choi ),김선림 ( Sunlim Kim ),강현중 ( Hyeonjung Kang ),김명환 ( Myunghwan Kim ),김우경 ( Wookyoung Kim ) 대한영양사협회 2019 대한영양사협회 학술지 Vol.25 No.3

There have been no published studies concerning the anti-inflammatory effects of corn silk on colon cancer cells. Thus, this study was conducted to investigate the effect of corn silk extract containing high levels of maysin on inflammation and its mechanism of action in colon cancer cells. SW 480 human colon cancer cells were treated with 1 μg/mL of lipopolysaccharide (LPS) to induce inflammation, and next they were treated with different concentrations of corn silk extract (0, 5, 10 and 15 μg/mL). The concentrations of nitric oxide (NO) were determined. The mRNA expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor α (TNF-α), interleukin- 1beta (IL-1β) and interleukin-6 (IL-6), were determined. Western blot analysis was performed to determine the protein expressions of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases, and the latter consists of extracellular signal-related kinase (ERK), c-jun NH2-terminal kinase (JNK) and p38 MAP kinase (p38). The concentration of NO and the mRNA expression of iNOS were significantly and dose-dependently decreased in the corn silk-treated groups (P<0.05). The mRNA expression of TNF-α, IL-1β and IL-6 were significantly increased in the LPS-treated group (P<0.05), but these expressions were significantly and dose-dependently decreased in the corn silk treated groups (P<0.05). The protein expressions of NF-κB (in a dose-dependent fashion), ERK (at 10 and 15 μg/mL), JNK (at 15 μg/mL) and p38 (at 10 and 15 μg/mL) were significantly decreased with corn silk treatments (P<0.05). In conclusion, corn silk extract containing high levels of maysin seems to inhibit the LPS-induced inflammatory responses in SW480 colon cancer cells via the NF-κB pathway.

Corrosion-Assisted Self-Growth of Au-Decorated ZnO Corn Silks and Their Photoelectrochemical Enhancement

Zhang, Zhuo,Choi, Mingi,Baek, Minki,Deng, Zexiang,Yong, Kijung American Chemical Society 2017 ACS APPLIED MATERIALS & INTERFACES Vol.9 No.4

<P>Modem nanotechnology generates more stringent requirements for the design and synthetic strategy of nanostructural materials. In this work, we demonstrate a novel strategy for the synthesis of 'corn silk'-like ZnO hierarchical nanostructures, simplified as ZnO corn silk: silk-like ZnO nanotubes (NTs) with a large length-to-diameter ratio are grown on the top tip of corn-shaped ZnO nanorods (NRs). The synthetic method is unique in that when the ZnO NRs are dipped into the aqueous solution of NaBH4, the release of Zn2+ and OH- caused by the corrosion of ZnO NRs, as well as the subsequent growth of, ZnO NTs, could allow the process to run step-by-step in self-assembly mode. This process is directed and driven by the change in concentrations of hydrogen anion H(s)(-) induced by NaBH4, as well as hydroxyl ions (OH-) induced by the H- formation and hydrolysis of dissociative Zn atoms. The prepared ZnO corn silks exhibit highly enhanced photoelectrochemical (PEC) efficiency after decoration with Au nanoparticles (NPs). ZnO silks act as pathways to facilitate efficient charge transfer, and the Au NP decoration induces the plasmonic effect, causing the hot electrons to inject into ZnO under visible illumination. At the same time, the formation of a Schottky barrier at the Au/ZnO interface can retard the electron-hole recombination. Overall, Au-decorated ZnO corn silk with an increased PEC efficiency represents a promising photoanode material, and the synthesis route developed in the current study is applicable to building hierarchical nanostructures of other materials.</P>

옥수수수염 분말의 입자크기별 이화학적 특성과 항산화활성

차선미,손범영,이진석,백성범,김선림,구자환,황종진,송범헌,우선희,권영업,김정태 한국작물학회 2012 한국작물학회지 Vol.57 No.1

본 연구에서는 옥수수수염을 일반분쇄기와 저온초미분쇄기를 이용하여 얻은 5단계의 다른 입자 크기의 옥수수수염 분말을 각각의 입자 크기별 이화학적 특성을 검토하고 항산화활성을 평가하여 최적의 입자크기를 찾고 우수한 가공법을 확립하고자 연구를 수행하였다. 본 연구의 결과를 요약하면 다음과 같다. 1. 건조된 옥수수수염을 일반적 분쇄 방법과 저온초미분쇄기를 이용하여 분쇄한 5단계의 입자크기는 각각 199.17um , 178.27um , 85.48um , 27.04um 및 20.97um 로 가장 큰 입자 크기와 가장 작은 입자 크기와는 약 10배 가량 입자크기의 차이가 있었다. 2. 입자크기별 옥수수수염 분말의 색차는 입자의 크기가 작아질수록 명도와 황색도의 수치가 커졌다. 3. 또한 입자크기별 옥수수 수염 분말을 주사전자현미경(SEM)을 이용하여 300배 확대하여 관찰한 결과 입자의 크기가 작아질수록 확대한 입자의 크기가 작은 것을 확인할 수 있었으며 입자의 모양은 다각형의 모양을 보였다. 4. 입자크기별 옥수수수염 분말의 조지방, 조섬유, 조회분, 조단백질 함량과 유리당 함량을 분석한 결과 조섬유 함량과 조단백질 함량은 입자크기가 작아질수록 그 수치가 줄어들었고, 조지방 함량과 조단백질 함량, 유리당 함량은 입자크기 간에 유의적인 차이를 보이지 않았다. 5. 입자크기별 옥수수수염 분말의 총 페놀 함량과 DPPH 라디컬 소거능 모두 입자크기가 작아질수록 그 수치가 증가하였고 통계적으로도 유의적인 차이를 보였다. 6. 옥수수수염 분말의 입자크기에 따른 ascorbic acid equivalents는 입자크기간의 수치적으로는 차이가 있는 것으로 보였으나 통계적으로는 서로간의 유의적 차이가 없는 것으로 나타났으나 TEAC 실험과 FRAP 실험에서는 입자크기가 작아질수록 그 수치가 증가하였고 통계적으로도 유의적인 차이를 보였다. 7. 옥수수수염 분말의 입자크기, 색도, 이화학적 특성 및 항산화 활성간의 상관관계를 분석한 결과 서로 유의적 관계를 보였으며 특히 입자크기가 작아질수록 명도, 적색도, 황색도, 총 페놀 함량, ascorbic acid equivalents, TEAC에서 고도로 유의한 상관관계를 보였다. The study was carried out to analyze the relationship between analysis of antioxidant activity and the level of functional components according to particle size of corn silk. Particle size was classified into 5 groups. By particle size distribution and color difference, the total phenol content and DPPH radical scavenging activity were observed. The particle sizes of corn silk were 199.17~mum , 178.27~mum , 85.48~mum , 27.4~mum and 20.97~mum , respectively. The lightness of colored pigments was increased when the particle size was decreased. The contents of free sugar (fructose, glucose, galactose, sucrose, and maltose) of corn silk were analyzed using a HPLC. The total phenol contents by the particle sizes of corn silk were 2.01 mg/g, 2.02 mg/g, 2.06 mg/g, 2.26 mg/g and 2.26 mg/g, respectively. DPPH radical scavenging activities of samples were 21.00%, 21.75%, 22.90%, 24.35% and 23.67%, respectively. Antioxidative activities of Trolox and Fe(II) in corn silk were measured by ferric reducing antioxidant power (FRAP) assay and Trolox equivalent antioxidant capacity (TEAC) assay. TEAC values of samples were 2.36~mumol TE / g dw, 2.81~mumol TE / g dw, 3.20~mumol TE / g dw, 3.36~mumol TE / g dw, and 3.44~mumol TE / g dw, respectively. FRAP values of samples were 11.67~mumol Fe(II) / g dw, 12.80~mumol Fe(II) / g dw, 13.43~mumol Fe(II) / g dw, 13.85~mumol Fe(II) / g dw and 15.95~mumol Fe(II) / g dw, respectively. Total phenolic content and antioxidantive activities based on FRAP assay and TEAC assay were increased with decreasing particle size. In addition, DPPH radical scavenging activity was also increased. A significant correlation was also noted between DPPH radical scavenging activities and the content of phenolic compounds.

자색 옥수수 잎과 줄기를 이용한 직물의 천연염색

김정태,손범영,이진석,백성범,김선림,김미정,정건호,권영업 韓國作物學會 2013 한국작물학회지 Vol.58 No.2

본 연구는 자색 옥수수 부산물인 옥수수 줄기와 잎을 이용하여 염액을 추출하여 여러 가지 염색 조건에서 염색정도를 관찰하고 자색옥수수 부산물의 염료로서의 가능성을 타진하여 이용성 증진을 위한 기초자료를 제공하고자 실시하였다.1. 자색 옥수수 추출시 pH에 따른 흡광도의 변화가 없으므로 자색 옥수수 색소 함량을 측정하기 위하여 UV흡광도를 측정할 때 520∼560 nm를 이용하는 것이효율적일 것으로 생각된다.2. 자색 옥수수 잎 추출물로 염색한 silk와 cotton은 pH가높아질수록 직물의 밝기와 적색도가 낮아졌다. 줄기추출물의 경우에는 뚜렷한 경향을 나타내지는 않았지만 pH 값이 높아질수록 밝기와 적색도가 높아지는 경향을 나타내었다.3. 농도에 따른 직물의 염색 정도는 silk와 cotton 모두 농도가 높아질수록 밝기는 낮아지고 적색도는 증가하는경향을 나타내었다.4. Silk와 cotton은 일반적으로 염색시간이 길어질수록명도가 증가하고, 적색도는 감소하는 경향을 나타내었다. This study was carried out to evaluate the dyeing possibility of purple corn stem and leaf. The UV absorbance of the purple corn stem extract and leaf extract at different pH does not change. It is considered to use to efficiently measure the purple corn pigment content when using UV absorbance at 520∼560 nm. By adjusting higher pH values in the extraction dyed fabrics, silk and cotton fabric used by the purple corn leaf extraction decreased lightness and redness. Extent for dyeing the fabric depending on the concentration, lightness and redness showed a tendency to increase color values when silk and cotton concentration increases. Also when the longer the dyeing time, silk and cotton increased lightness but redness decreased.

옥수수 수염에서 Maysin 및 유사물질의 동정

김선림,Maurice E. Snook,김이훈,박철호 韓國作物學會 2000 한국작물학회지 Vol.45 No.3

옥수수수염에 함유되어 있는 maysin 및 유사 flavonoid 물질의 분리 및 정제법을 확립하여 신품종 육성의 기초적인 자료를 제공하고자 본 시험을 수행하였으며 얻어진 결과를 요약하면 다음과 같다. 1. Preparative C18 컬럼에서 10% MeOH에 용출된 물질은 neochlorogenic acid, chlorogenic acid 및 4-caffeoylquinic acid였으며 30% MeOH로 용출된 물질은 rhamnosyl isoorientin이었고 maysin은 50%의 MeOH에서 용출되었다. 2. Silicic acid 컬럼으로 maysin 조추출물의 1차 정제시 100% ethyl acetate 500m 로 컬럼에 흡착되어 있는 maysin을 용출시켰으며, 이때 수거된 maysin의 순도는 75% 이상에 해당하였고, C18 컬럼(12 43")으로 maysin의 2차 정제시 maysin의 순도는 95% 이상에 달하였다. 3. FAB-MS에 의한 maysin의 분자량은 577M+H m/z이고, fragmentation으로 보아 431M+H m/z은 rhamnose에 해당하였고, 1 H 및 13C NMR에 의한 spectrum을 확인한 결과 분리한 물질이 maysin임을 확인할 수 있었다. 있었다. This study was carried out to isolate and identify the maysin and related flavonoid analogues in corn silks. Silks were covered with silk bag to prevent pollination and were sampled at 3-5 days after silking. The silks were filled with 100% MeOH and stored at 0~circC until analysis. The MeOH extracts of corn silks were filtered and concentrated at 35-40~circC . The CH2 Cl2 was added on the concentrated aqueous solution to remove the chlorophyll and lipids. The Cis open column (25mm~times 54 cm) was washed and activated with serial treatment of 500m~ell of 100% MeOH(twice)longrightarrow75% MeOH longrightarrow50% MeOHlongrightarrow30% MeOHlongrightarrow100% H2 O(2 times). The concentrated aqueous solution was applied to the C18 column and washed with H2O several times to remove the sugars and water soluble pigments. Neochlorogenic acid, chlorogenic acid and 4-caffeoylquinic acid were eluted with 10% MeOH, and rhamosyl isoorientin was eluted with 30% MeOH, but maysin was eluted with 50% MeOH from the C18 open column. Collected fractions were analyzed with HPLC by using revers-phase Ultras-phere C18 column (4.6~times 250mm, 5~mu~textrmm ) and H2 O (10% MeOH containing 0.1% H3 PO4 )/MeOH (100% MeOH containing 0.1% H3 PO4 ) linear gradient from 20% to 90% MeOH for 35 minutes, a flow rate of 1 m~ell /min and detection at 340nm. The selected fractions were concentrated and applied to the silicic acid column. Maysin was eluted with 500m~ell of 100% ethyl acetate from the silicic acid column for the first purification, and the purity of collected fractions was about 75%, but the purity from the second purification with the Cis column (1/2 ~times 43") was greater than 95%. FAB-MS spectral data was obtained with VG7O-VSEQ VG analytical fast atom bombardment mass (UK). 1 H-NMR and 13 C-NMR data were obtained with Bruker DPX 400 MHz NMR spectrometers (German) in DMSO-d6 at 400 and 100 MHz