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콩품종의 Oligosaccharide 함량과 발아에 따른 변화
김선림,문중경,윤홍태,박금룡,이영호,유용환,구자환,김석동 한국육종학회 2002 한국육종학회지 Vol.34 No.3
This study was carried out to establish the analysis condition for oligosaccharides of soybeans (Glycine max L.) and to assay quantitative evaluation of oligosaccharide contents in soybean varieties and soybean sprouts. 55% acetonitrile solution used as a
김선림,최희원,김정태,허온숙,김덕수,서세정,김현복,정일민 한국작물학회 2011 Korean journal of crop science Vol.56 No.4
The objectives of present study were to characterize the peptides which were isolated from Korean fermented soybean paste, chungkukjang, and to determine their antioxidant activities. Four fractions were collected from the methanol extract of chungkukjang by using a recycling preparative HPLC. Among fractions, Fr-2 was identified to be highly potent free radical scavenging activity in the assay of 1,1-diphenyl-2-picryl-hydrazyl (DPPH)and nitroblue tetrazolium(NBT)-reduction inhibition. Base on antioxidant effects, fraction Fr-2 was employed for the refraction with a prep-column and separated into five fractions of which two fractions were identified to have higher antioxidant activity. To confirm the amino acid constituents of antioxidant fractions Fr-2-2 and Fr-2-3 were analyzed,and eight kinds of amino acids such as aspartic acid, threonine,serine, glutamic acid, glycine, lysine, histidine, and arginine were identified as the constituent amino acids. Antioxidant activities of the separated peptides were further assessed cell viability with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl terazolium bromide (MTT), and fluorescence-activated cell sorting (FACS) analysis of H4IIE cells treated with hydrogen peroxide (H2O2). Chungkukjang peptides have shown their ability to protect H4IIE rat hepatoma cells against H2O2-induced oxidative stress by concentration and time-dependent manner. Therefore, These results indicated that fermented soybean paste chungkukjang will be promoted the antioxidant and radical scavenging activities, and beneficial for health. The antioxidant peptide fractions Fr-2-2 and Fr-2-3 were denominated as P-NICS-1 and P-NICS-2, respectively. However,further studies were required to clarify their amino acid sequences and molecular properties, and physiological significances.