중국 산시성 30대 여성의 피부노화 인식과 항노화 관리 특성

송효정,최미옥 한국미용학회 2022 한국미용학회지 Vol.28 No.6

This study aims to provide accurate information through data on the perception of skin aging and anti-aging management behavior characteristics of women in their 30s in Shanxi Province, China, and to provide basic data on the development and professional management of cosmetics tailored to customers' needs in the anti-aging market. For the study, an electronic questionnaire was produced by the Wenjuanxing program, and the purpose of the study and the survey contents were read to a woman in her 30s living in Shanxi Province, China, and a survey was conducted anonymously. A total of 260 copies were distributed, and 227 copies were finally analyzed except for 33 responses that were insincere or omitted. The survey was divided into three parts: 6 questions to know the general characteristics of the survey subjects, 19 questions to know skin aging awareness, 8 questions to know anti-aging cosmetics use characteristics, and 9 questions to know professional care use characteristics among anti-aging management. The collected data were analyzed using the SPSSWIN21.0 program for Windows. The results of the study showed that women in their 30s in Shanxi Province, China, are usually aware of skin aging, but there is a lot of interest in anti-aging management. To prevent skin aging, cosmetics are used, lifestyle is maintained well, and the period of using cosmetics is 20~25 years old. Frequently used cosmetics are ‘moisturizing products’, and cosmetics are important to improve skin aging. In addition, “self-management (home care)” is the most common professional management, and “removing wrinkles” is the most common management. These findings show that women in their 30s need to spread knowledge about skin aging, and there is a growing interest in skin aging and anti-aging management by identifying their skin characteristics and selecting skin care that suits them. Therefore, professional management should develop a skin care program for middle-aged women in their 30s by developing a skin care program tailored to each age group, and the results of this study are meaningful in providing basic data on the dissemination of anti-aging knowledge and development and marketing of Chinese middleaged women.

Adipose-Derived Mesenchymal Stem Cells and Their Derived Epidermal Progenitor Cells Conditioned Media Ameliorate Skin Aging in Rats

Badr Omar I.,Anter Aya,Magdy Ihab,Chukueggu Marvellous,Khorshid Moamen,Darwish Mohamed,Farrag Mohamed,Elsayed Menna,Amr Youmna,Amgad Yomna,Mahmoud Tasnim,Kamal Mohamed M. 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.6

BACKGROUND: Skin alterations are among the most prominent signs of aging, and they arise from both intrinsic and extrinsic factors that interact and mutually influence one another. The use of d-galactose as an aging model in animals has been widely employed in anti-aging research. Adipose tissue-derived mesenchymal stem cells (Ad-MSCs) are particularly promising for skin anti-aging therapy due to their capacity for effective re-epithelization and secretion of various growth factors essential for skin regeneration. Accordingly, we aimed to examine the potential utility of Ad-MSCs as a therapy for skin anti-aging. METHODS: In this study, we isolated and characterized adipose-derived mesenchymal stem cells (Ad-MSCs) from the epididymal fat of male Sprague Dawley rats. We assessed the in vitro differentiation of Ad-MSCs into epidermal progenitor cells (EPCs) using ascorbic acid and hydrocoritsone. Additionally, we induced skin aging in female Sprague Dawley rats via daily intradermal injection of d-galactose over a period of 8 weeks. Then we evaluated the therapeutic potential of intradermal transplantation of Ad-MSCs and conditioned media (CM) derived from differentiated EPCs in the d-galactose-induced aging rats. Morphological assessments, antioxidant assays, and histopathological examinations were performed to investigate the effects of the treatments. RESULTS: Our findings revealed the significant capability of Ad-MSCs to differentiate into EPCs. Notably, compared to the group that received CM treatment, the Ad-MSCs-treated group exhibited a marked improvement in morphological appearance, antioxidant levels and histological features. CONCLUSIONS: These results underscore the effectiveness of Ad-MSCs in restoring skin aging as a potential therapy for skin aging. BACKGROUND: Skin alterations are among the most prominent signs of aging, and they arise from both intrinsic and extrinsic factors that interact and mutually influence one another. The use of d-galactose as an aging model in animals has been widely employed in anti-aging research. Adipose tissue-derived mesenchymal stem cells (Ad-MSCs) are particularly promising for skin anti-aging therapy due to their capacity for effective re-epithelization and secretion of various growth factors essential for skin regeneration. Accordingly, we aimed to examine the potential utility of Ad-MSCs as a therapy for skin anti-aging. METHODS: In this study, we isolated and characterized adipose-derived mesenchymal stem cells (Ad-MSCs) from the epididymal fat of male Sprague Dawley rats. We assessed the in vitro differentiation of Ad-MSCs into epidermal progenitor cells (EPCs) using ascorbic acid and hydrocoritsone. Additionally, we induced skin aging in female Sprague Dawley rats via daily intradermal injection of d-galactose over a period of 8 weeks. Then we evaluated the therapeutic potential of intradermal transplantation of Ad-MSCs and conditioned media (CM) derived from differentiated EPCs in the d-galactose-induced aging rats. Morphological assessments, antioxidant assays, and histopathological examinations were performed to investigate the effects of the treatments. RESULTS: Our findings revealed the significant capability of Ad-MSCs to differentiate into EPCs. Notably, compared to the group that received CM treatment, the Ad-MSCs-treated group exhibited a marked improvement in morphological appearance, antioxidant levels and histological features. CONCLUSIONS: These results underscore the effectiveness of Ad-MSCs in restoring skin aging as a potential therapy for skin aging.

Molecular Mechanisms and In Vivo Mouse Models of Skin Aging Associated with Dermal Matrix Alterations

Kyung-A Hwang,Bo-Rim Yi,Kyung-Chul Choi 한국실험동물학회 2011 Laboratory Animal Research Vol.27 No.1

Skin is the most superficial body organ and plays an important role in protecting the body from environmental damage and in forming social relations. With the increase of the aging population in our society, dermatological and cosmetic concerns of skin aging are rapidly increasing. Skin aging is a complex process combined with intrinsic and extrinsic factors. Intrinsic or chronological skin aging results from the passage of time and is influenced by genetic factors. Extrinsic skin aging is mainly determined by UV irradiation, also called photoaging. These two types of aging processes are superimposed on sunexposed skin, and have a common feature of causing dermal matrix alterations that mostly contribute to the formation of wrinkles, laxity, and fragility of aged skin. The dermal matrix contains extracellular matrix proteins such as collagen, elastin, and proteoglycans that confer the strength and resiliency of skin. Skin aging associated with dermal matrix alterations and atrophy can be caused by cellular senescence of dermal cells like fibroblasts, and decreased synthesis and accelerated degradation of dermal matrix components, especially collagen fibers. Both intrinsic aging and photoaging exert influence during each step of dermal matrix alteration via different mechanisms. Mouse models of skin aging have been extensively developed to elucidate intrinsic aging and photoaging processes, to validate in vitro biochemical data, and to test the effects of pharmacological tools for retarding skin aging because they have the advantages of being genetically similar to humans and are easily available.

중국 성인여성의 피부관리 행동 및 피부관리실 이용실태에 따른 피부노화 인식과의 관련성

왕옥,김경란 아시아.유럽미래학회 2024 유라시아연구 Vol.21 No.2

본 연구는 중국 성인여성들을 대상으로 피부관리 행동과 피부관리실 이용실태에 따른 피부노화 인식과의 관련성을 검증하고자 2024년 03월 19일부터 03월 21일까지 위쳇(WeChat)을 이용하여전자 설문한 269부를 연구에 사용하였다. 조사된 자료는 엑셀프로그램(WPS Excel 2022 program)으로 자동코딩된 자료를 SPSS v. 25.0 통계 패키지 프로그램을 이용하여 빈도분석을 실시하였고, 피부노화 인식은 요인분석과 신뢰도 검증을 위해 Cronbach's α계수를 산출하였다. 차이검증을 위해 독립표본t-test 및 일원변량분석(One way ANOVA)을 실시하였다. 일반적인 특성의 연령은 25∼35세, 결혼여부는 기혼, 최종학력은 전문대학, 직업은 전문직, 월 평균소득은 5,000 미만, 피부유형은 복합성피부, 피부고민은 넓은 모공, 피부노화 진행 원인은 관리소홀과식습관, 정보습득은 인터넷/모바일이 가장 높게 나타났다. 피부노화 예방을 위한 피부관리 행동은 각질제거, 선크림 사용, 충분한 숙면 노력한다에서 기장 높게 나타났다. 피부관리방법은 세안흿수는 2회, 스킨/로션 사용횟수는 2회, 항노화 화장품 사용, 시작시기는 20∼25세, 화장품 유형은 수분제품, 항노화제품 사용하지 않은 이유로 번거러워서가 가장 높게 나타났다. 일반적인 특성에 따른 피부노화 인식의 차이에서 연령이 낮을수록, 학력이 높을수록 피부노화 원인인식이 통계적으로 유의미하게 나타났다. 피부관리 행동에 따른 피부노화 인식에서는 화장품 사용 시작시기에서 연령이 낮을수록 피부노화 원인을 인식하고 피부관리 행동이 중요하다로 응답하여 통계적으로 유의미한 차이가 나타났다. 일반적으로 연령이 높을수록 피부노화 인식 및 개선이 높다고 보고하고 있으나, 본 연구에서 연령이 낮은 20대에서 피부노화 원인 및 인식 개선이 높게 나타났다. 따라서연령이 낮은 청소년부터 항노화 예방법, 피부관리 행동, 피부관리실 이용목적 등을 위한 정보제공과 마케팅의 필요성을 제시하고자 한다. This study conducted an electronic survey using WeChat from March 19, 2024 to March 21, 2024, to verify the relationship between skin care behavior and awareness of skin aging according to skin care salon use among Chinese adult women. . The surveyed data were automatically coded in Excel program format using SPSS v. Frequency analysis was conducted using the 25.0 statistical package program, and Cronbach's α coefficient was calculated for factor analysis and reliability verification for skin aging awareness. To verify the difference, independent samples t-test and one-way ANOVA were performed. General characteristics, age range from 25 to 35 years old, whether married or not, final education in college (associate degree), occupation as a full-time worker, monthly average income less than 5000, skin type complex skin, skin problems with enlarged pores, lack of management and dietary habits due to skin aging, and the highest access to information is through the internet/mobile phone. As a living habit of preventing skin aging and skin management, strive to remove horniness, use sunscreen, and get enough sleep. The number of exercises is 1-2 times a week, without the use of facial mask. The average daily sleep time is 5-7 hours. The skin management method is to wash your face twice, use toner/lotion twice, and use anti-aging cosmetics from the age of 20 to 25. The type of cosmetics is water products, and anti-aging products are not used, because it is cumbersome. In the understanding of skin aging based on skin management actions, the younger the age at the beginning of cosmetic use, the more aware the causes of skin aging, and the more important the skin management actions are, resulting in statistically significant differences. According to the usage of skin management, although there is no statistically significant difference in understanding skin aging, the higher the frequency of use in the skin management room, the lower the amount of management fees, and the more people who answer the need to improve the causes and understanding of skin aging.

피부노화에 대한 인식정도와 관리행동에 관한 연구

김민재,김현이 한국뷰티경영학회 2021 한국뷰티경영학회지 Vol.9 No.2

This study conducted a survey on 211 adults residing in Seoul and Gyeonggi Province to find out the level of awareness and management behavior for skin aging. The results of the study are as follows. As for the skin type, combination skin accounted for the most at 30.8%, and the first time of skin aging was from 25 years old to less than 30 years old. management was required. In the questionnaire about interest in skin aging management, 35.6% answered 'yes', and about the use of cosmetics to prevent skin aging, 56.6% and 43.1% answered 'yes'. The most frequently used cosmetics for this purpose were moisturizing products. In the questionnaire on whether to use skin care, there were more respondents who did not use skin care, and 61.7% of the respondents spent less than 100,000 won on skin care to improve the average annual skin aging. As a result of investigating the awareness level and management behavior of skin aging, it can be analyzed that people in their 20s and 30s, who are familiar with the media, are paying attention to skin care, although they feel and are interested in skin aging, but are not trying to improve it. have. Therefore, it is meaningful to seek the right information and improvement plans through this study, and to provide basic data for the correct anti-aging management method and professional skin care office and anti-aging market in the future.

피부노화의 주관적 인지에 따른 항노화인식, 뷰티관리행동, 피부관리실 선택속성 조사

박미승,권기한 한국미용예술경영학회 2023 미용예술경영연구 Vol.17 No.4

This study aims to clarify the target of skin aging-related product development in the beauty industry or establish marketing strategies to secure customers in the skin care shops by examining the subjective perception of skin aging in women in their 30s and 60s. This survey collected data from adult women in their 30s to 60s through the Internet and direct surveys from December 14 to December 20, 2022, and a total of 770 copies of the questionnaire were distributed and 662 copies, excluding 101 unfaithful copies, were used as final analysis data. Frequency analysis, factor analysis, reliability analysis, t-test verification, one-way batch variance analysis, and Scheffe follow-up analysis were conducted on the collected data using the SPSS 28.0 program. Based on the subjective perception of skin aging, skin aging is first recognized and is most concerned about skin aging due to skin color changes from the age of 20-24 through differences in anti-aging perception, beauty care behavior, and skin care shops selection attributes. Based on these results, it is believed that it can be used as basic data for marketing the beauty industry to stimulate the purchase of products for beauty care to improve skin aging and the promotion of skin care shops purchase. In the study, it was found that the onset of skin aging was recognized as 20-24 years old, so it is suggested that follow-up studies including those in their 20s will be conducted.

Inflammaging and Barrier Function in Aged Skin

( Eung Ho Choi ),( Sung Ha Lim ),( Beom Jun Kim ),( Hyun Jee Hwang ) 한국피부장벽학회 2021 한국피부장벽학회지 Vol.23 No.2

Inflammaging is a chronic low-level inflammation associated with aging and drive many age-associated diseases including atherosclerosis, type 2 diabetes, and Alzheimer’s disease. In inflammaging, increased levels of circulating proinflammatory cytokines such as IL-6, IL-8 and TNF-α are observed. In addition, glucocorticosteroid hormone (GC), which has anti-inflammatory effects, is increased in the blood, skin, and mucosa of aging humans and mice. The expression of 11β-hydroxysteroid dehydrogenase type 1 (HSD1), which converts inactive GC to active GC, increases with aging and contributes to the phenotypes of aged skin. In aging skin, barrier function is impaired by various factors. GC activated by 11β-HSD1 may further deteriorate the skin barrier function in aged skin. Stratum corneum lipid levels were decreased in aged skin, however this could be rescued by treatment with an 11β-HSD1 inhibitor. The three main mechanisms responsible for skin pH are disturbed with age. Among them, abnormal function of sodium-hydrogen exchanger 1 (NHE1) is a specific underlying defect in the pH increase in aging skin. Repair strategies for the aging skin barrier are physiological lipid mixtures, NHE1 activators, and acidic moisturizers. Inflammaging and barrier dysfunction are observed in the aged or diabetic skin. The long-standing hyperglycemia observed in type 2 diabetes accelerate the skin aging process, damaging the skin barrier. In aging skin, impaired barrier function increases the cutaneous pro-inflammatory cytokines, both of which are restored by moisturizers. Conversely, inflammaging exacerbates barrier dysfunction. In summary, skin barrier impairment in the aged may lead to skin inflammation, which in turn may lead to systemic inflammation by increasing blood cytokines. Therefore, inflammaging and its sequelae may be prevented or mitigated with barrier repair strategies.

Inflammaging and Barrier Function in Aged Skin

( Eung Ho Choi ),( Sung Ha Lim ) 한국피부장벽학회 2021 한국피부장벽학회지 Vol.23 No.2

Inflammaging is a chronic low-level inflammation associated with aging and drives many age-associated diseases including atherosclerosis, type 2 diabetes, and Alzheimer’s disease. In inflammaging, increased levels of circulating proinflammatory cytokines such as IL-6, IL-8, and TNF-α are observed. In addition, glucocorticosteroid hormone (GC), which has anti-inflammatory effects, is increased in the blood, skin, and mucosa of aging humans and mice. The expression of 11β-hydroxysteroid dehydrogenase type 1 (HSD1), which converts inactive GC to active GC, increases with aging and contributes to the phenotypes of aged skin. In aging skin, barrier function is impaired by various factors. GC activated by 11β-HSD1 may further deteriorate the skin barrier function in aged skin. Stratum corneum lipid levels were decreased in aged skin, however, this could be rescued by treatment with an 11β-HSD1 inhibitor. The three main mechanisms responsible for skin pH are disturbed with age. Among them, the abnormal function of sodium-hydrogen exchanger 1 (NHE1) is a specific underlying defect in the pH increase in aging skin. Repair strategies for the aging skin barrier are physiological lipid mixtures, NHE1 activators, and acidic moisturizers. Inflammaging and barrier dysfunction are observed in the aged or diabetic skin. The long-standing hyperglycemia observed in type 2 diabetes accelerates the skin aging process, damaging the skin barrier. In aging skin, impaired barrier function increases the cutaneous pro-inflammatory cytokines, both of which are restored by moisturizers. Conversely, inflammaging exacerbates barrier dysfunction. In summary, skin barrier impairment in the aged may lead to skin inflammation, which in turn may lead to systemic inflammation by increasing blood cytokines. Therefore, inflammaging and its sequelae may be prevented or mitigated with barrier repair strategies.

피부노화의 발병기전 및 예방

조소연 대한의사협회 2021 대한의사협회지 Vol.64 No.6

Background: As global life expectancy increases, an interest in maintaining health and beauty in old age has increased. As a barrier organ, the skin is an ideal model for studying both genetically-programmed (intrinsic) and environmentallyinduced (extrinsic) aging. Current Concepts: Among the extrinsic aging factors, solar ultraviolet radiation is the most important, accounting for 80% of facial skin aging. Other nongenetic factors include air pollution, cigarette smoke, nutrition, temperature, sleep, and stress. Through complex interplay, genome, exposome and microbiome all contribute to skin aging. Intrinsic aging causes thinning of the skin and fine wrinkles, while extrinsic aging leads to thick rubbery skin texture, deep wrinkles and dyspigmentation in exposed areas. Fibroblast senescence is a fundamental mechanism of skin aging, with these cells persisting and exhibiting a senescence-associated secretory phenotype which secrets proinflammatory cytokines. Chronic low-level inflammation associated with aging, termed inflamm-aging, is exacerbated by oxidative damage caused by extrinsic factors. Discussion and Conclusion: Understanding the pathogenesis of skin aging may help in developing anti-aging strategies in general. In addition to applying sunscreen every morning and retinoic acid every night, taking antioxidantrich foods and maintaining a healthy lifestyle are all important for preventing skin aging.

P007 Changes of decorin and biglycan in intrinsically aged and photoaged human skin

( Jang-hee Oh ),( Hanon Lee ),( Min Kyeong Shin ),( Ji Soo Lim ),( Soyun Cho ),( Jin Ho Chung ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.2

<div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div> Background: Loss of mature collagen and increase of matrix metalloproteinase expression are the major molecular change during skin aging processes; however, change of glycosaminoglycans (GAGs) and proteoglycans in skin aging processes has not been well elucidated. Objectives: To investigate the skin aging-related changes of decorin and biglycan, including their protein expression levels and GAG chain length in intrinsically aged and photoaged human skin. Methods: Skin samples of buttock and forearm skin were obtained from young (20~40 yr, n=6) and aged volunteers (70~78 yr, n=9). Expression level of decorin and biglycan was investigated with real-time RT-PCR and Western blot analysis. Size shift to their core proteins was confirmed by treatment with chondroitinase ABC. Results: mRNA level of decorin and biglycan was not significantly reduced during skin aging. In contrast, by Western blot analysis of dermal protein samples, biglycan levels were significantly reduced in intrinsically aged buttock dermis, compared to young buttock dermis, while decorin levels did not. Sizes of decorin and biglycan were decreased in intrinsically aged buttock skin; however, their sizes were increased in aged forearm skin, compared to each individual’s buttock skin. Conclusion: Reduction of biglycan levels and the GAG chain length changes of decorin and biglycan in aged dermis may give some clues for explanation of aging-related skin phenotypes.