SMV코덱의 음성/음악 분류 성능 향상을 위한 Support Vector Machine의 적용

김상균(Sang-Kyun Kim),장준혁(Joon-Hyuk Chang) 대한전자공학회 2008 電子工學會論文誌-SP (Signal processing) Vol.45 No.6

본 논문에서는 support vector machine (SVM)을 이용하여 기존의 3GPP2 selectable mode vocoder (SMV)코덱의 음성/음악 분류 성능을 향상시키는 방법을 제시한다. SVM은 통계적 학습 이론으로 훈련 데이터 사이의 최적 분류 초평면을 찾아내 최적화된 이진 분류를 보여준다. SMV의 음성/음악 실시간 분류 알고리즘에서 사용된 특징벡터와 분류방법을 분석하고, 이를 기반으로 분류성능향상을 위해 통계적 학습 이론인 SVM을 도입한다. 구체적으로, SMV의 음성/음악 분류알고리즘에서 사용되어진 특징벡터만을 선택적으로 사용하여 효과적으로 SVM을 구성한 분류기법을 제시한다. SMV의 음성/음악 분류에 적용한 SVM의 성능 평가를 위해 SMV 원래의 분류알고리즘과 비교하였으며, 다양한 음악장르에 대해 시스템의 성능을 평가한 결과 SVM을 이용하였을 때 기존의 SMV의 방법보다 우수한 음성/음악 분류 성능을 보였다. In this paper, we propose a novel approach to improve the performance of speech/music classification for the selectable mode vocoder (SMV) of 3GPP2 using the support vector machine (SVM). The SVM makes it possible to build on an optimal hyperplane that is separated without the error where the distance between the closest vectors and the hyperplane is maximal. We first present an effective analysis of the features and the classification method adopted in the conventional SMV. And then feature vectors which are applied to the SVM are selected from relevant parameters of the SMV for the efficient speech/music classification. The performance of the proposed algorithm is evaluated under various conditions and yields better results compared with the conventional scheme of the SMV.

콩壞疽 바이러스(SMV-N) 罹病率에 있어서 品種間 差異와 播種期의 影響

Young Hyun Hwang(黃永鉉) 한국육종학회 1993 한국육종학회지 Vol.25 No.3

Epidemic necrotic soybean mosaic virus(SMV-N) was observed on the early planted soybean fields of Kyungbuk Provinical Rural Development Administration in the southern part of Korea where no record of severe SMV-N was made hitherto. Milyangkong which is successfully being cultivated without any SMV-N infection on farmer’s fields in the normal planting date of June 20 showed 88.7%, 38.9%, 25.8%, and 10.4% of SMV-N infection rates in the early planting dates of April 15, April 30, May 15, and May 30, respectively. A great deal of difference in infection rates of SMV-N among twenty tested soybean vareties as well as among three planting times, April 20, May 20, and June 20, were recognized. At the same time, high negative correlations between the infection rate of soybean mosaic virus(SMV) at flowering and that of SMV-N at maturity were also recognized at all three planting times. All American soybean varieties having indeterminated growth habits and some of Korean varieties such as Jangbaekkong, Namhaekong, Danwonking, and Eunhakong which are somewhat suceptible to SMV showed high resistance to SMV-N. Two Korean varities, Paldalkong and SS77053 were resistant to SMV and SMV-N simultaneously. Applying of the cross protection theorem is advisable in the development of soybean varieties for the upland-paddy field rotation croppings, which can be a means of avoiding the deveatating yield loss by possible epidemic SMV-N.

RNAi-mediated Soybean mosaic virus (SMV) resistance of a Korean Soybean cultivar

김혜정,김미진,박정훈,임현희,이동희,김국형,이재헌,김도훈,최홍규,정호원,정영수 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.5

Soybean [Glycine max (L.) Merr.] is an important crop for vegetable oil production, and is a major protein source worldwide. Because of its importance as a crop, genetic transformation has been used extensively to improve its valuable traits. Soybeanmosaic virus(SMV)is one of themostwellknown viral diseases affecting soybean. Transgenic soybean plants with improved resistance to SMV were produced by introducing HC-Pro coding sequences within RNA interference (RNAi) inducing hairpin construct via Agrobacteriummediated transformation. During an experiment to confirm the response of transgenic plants (T2) to SMV infection, no T2 plants from lines #2 (31/31), #5 (35/35) or #6 (37/37) exhibited any SMV symptoms, indicating strong viral resistance (R), whereas NT (non-transgenic wild type) plants and those from lines #1, #3 and #4 exhibited mild mosaic (mM) or mosaic (M) symptoms. The northern blot analysis showed that three resistant lines (#2, #5 and #6) did not show the detection of viral RNA accumulation while NT, EV (transformed with empty vector carrying only Bar) and lines #1, #3 and #4 plants were detected. T3 seeds from SMV-inoculated T2 plants were harvested and checked for changes in seed morphology due to viral infection. T3 seeds of lines #2, #5 and #6 were clear and seed coat mottling was not present, which is indicative of SMV resistance. RT-PCR and quantitative real-time PCR showed that T3 seeds fromthe SMV-resistant lines #2, #5 and #6 did not exhibit any detection of viral RNA accumulation (HC-Pro, CP and CI), while the viral RNA accumulation was detected in SMV-susceptible lines #1, #3 and #4 plants. During the greenhouse test for viral resistance and yield components, T3 plants from the SMV-inoculated transgenic lines #2, #5 and #6 showed viral resistance (R) and exhibited a more favorable average plant height, number of nodes per plant, number of branches per plant, number of pods per plant and total seed weight with statistical significance during strong artificialSMV infection than did other plant lines. In particular, the SMVresistant line #2 exhibited superior average plant height, pod number and total seed weight with highly significance. According to our results,RNAiinduced by the hairpin construct of the SMV HC-Pro sequence effectively confers much stronger viral resistance than did the methods used during previous trials, and has the potential to increase yields significantly. Because of its efficiency, the induction of RNAi-mediated resistance will likely be used more frequently as part of the genetic engineering of plants for crop improvement.

Molecular variability and genetic structure of the population of <i>Soybean mosaic virus</i> based on the analysis of complete genome sequences

Seo, Jang-Kyun,Ohshima, Kazusato,Lee, Hyeok-Geun,Son, Moonil,Choi, Hong-Soo,Lee, Su-Heon,Sohn, Seong-Han,Kim, Kook-Hyung Elsevier 2009 Virology Vol.393 No.1

<P><B>Abstract</B></P><P>The complete genomes of 30 <I>Soybean mosaic virus</I> (SMV) isolates and strains were sequenced in this study. Together with fourteen previously reported sequences, we analyzed the genetic structure of the SMV population. Analyses of genetic diversity showed that different genomic regions of SMV are under different evolutionary constraints and that there was no significant genetic differentiation between East Asian and North American populations of SMV. Phylogenetic analyses revealed a significant correlation between phylogeny of the cylindrical inclusion (CI) gene of SMV and SMV resistance gene 3 (<I>Rsv3</I>)-relating pathogenicity of SMV, suggesting CI might be a pathogenic determinant in <I>Rsv3</I>-mediated disease response. Interestingly, recombination analyses identified 19 ‘clear’ recombination events in the SMV population. Furthermore, as several resistance-breaking strains were identified as recombinants, it appears that recombination might contribute to overcome host resistance in SMV–soybean pathosystem. Our finding suggests that recombination as well as mutation is an important evolutionary process in the genetic diversification of SMV population.</P>

Infectious in vivo Transcripts from a Full-length Clone of Soybean mosaic virus Strain G5H

Seo, Jang-Kyun,Lee, Hyeok-Geun,Choi, Hong-Soo,Lee, Su-Heon,Kim, Kook-Hyung The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.1

An infectious full-length clone of Soybean mosaic virus (SMV) strain G5H was constructed under the control of the cauliflower mosaic virus 35S promoter. The cloned SMV G5H established infections upon simple rub-inoculation of soybean leaves with intact plasmid DNA. We demonstrated that this SMV G5H infectious DNA clone caused typical characteristic symptoms and virulence of SMV strain G5H in twelve tested soybean cultivars. Soybean cultivars Lee74, Somyungkong and Sowonkong developed systemic mosaic symptom while Kwanggyo, Taekwangkong, Hwangkeumkong and Geumjeongkong-l showed systemic necrosis. In contrast, Geumjeongkong-2, Jinpumkong-2, L29, V94-5152 and Ogden showed resistant response against SMV-G5H infection. We also determined full-length sequence of cloned SMV-G5H. The phyogenetic analyses reveal that SMV-G5H is most closely related to SMV-G5, and support that SMV-G5H might be derived from SMV-G5 by recombination rather than mutation.

3GPP2 SMV의 실시간 유/무성음 분류 성능 향상을 위한 Gaussian Mixture Model 기반 연구

송지현(Ji-Hyun Song),장준혁(Joon-Hyuk Chang) 대한전자공학회 2008 電子工學會論文誌-SP (Signal processing) Vol.45 No.5

본 논문에서는 패턴 인식에서 우수한 성능을 보이는 가우시안 혼합모델 (Gaussian mixture model, GMM)을 이용하여 비정상적인 잡음환경에서 3GPP2 selectable mode vocoder (SMV)의 유/무성음 분류 알고리즘 성능 향상을 위한 방법을 제안한다. 기존의 SMV에 대해서 분석하고, 이를 기반으로 유/무성음 분류 알고리즘에서 우수한 성능을 보여주는 특징 벡터를 선택하여 GMM의 입력벡터로 효과적으로 이용한다. 다양한 잡음환경에서 시스템의 성능을 평가한 결과 GMM을 이용한 제안된 방법이 기존의 SMV의 방법보다 우수한 유/무성음 분류 성능을 보였다. In this paper, we propose an approach to improve the performance of voiced/unvoiced (V/UV) decision under background noise environments for the selectable mode vocoder (SMV) of 3GPP2. We first present an effective analysis of the features and the classification method adopted in the SMV. And then feature vectors which are applied to the GMM are selected from relevant parameters of the SMV for the efficient voiced/unvoiced classification. For the purpose of evaluating the performance of the proposed algorithm, different experiments were carried out under various noise environments and yields better results compared with the conventional scheme of the SMV.

국내 콩에서 발생하는 바이러스 병해의 발생 상황

이영훈 ( Yeong Hoon Lee ),임승택 ( Seung Taek Lim ),윤영남 ( Young Nam Yoon ),전명기 ( Myeong Gi Jeon ),윤홍태 ( Hong Tae Yun ),고종민 ( Jong Min Ko ),이수헌 ( Su Heon Lee ),이기운 ( Key Woon Lee ),백인열 ( In Youl Baek ) 한국콩연구회 2013 韓國콩硏究會誌 Vol.29 No.1

국내 콩에서는 SMV, SbDV, AMV, CMV, CPMV, SYMMV, SYCMV와 PSV가 보고되었다. 과거 이들 바이러스 중에서 SMV가 심각한 피해를 입히고 있으며, 90%이상 우점하는 것으로 보고되었다. 하지만, 최근 SMV의 발생률은 50%정도로 낮아졌으며, SYMMV, SYCMV와 PSV 등에 의한 여러 가지 바이러스 병해가 피해를 주고 있는 것으로 확인되었다. 최근 기후 및 재배환경의 변화와 국제 농산물 교역으로 인해 병 발생 양상이 급속하게 변하고 있음을 나타내는 단적인 예인 것이다. 또한, 새로운 매개층과 전염원의 발생으로 신종 또는 미보고 바이러스 발생이 늘어나고 있지만, 콩과 같은 주요 작물의 병해 발생 상황 및 피해 양상 구명에 대한 연구는 미흡한 실정이다. 이러한 이유로 국내 콩에서 발생하는 바이러스 병해의 확인을 위해 8도19지역에서 193점의 시료를 채집하였다. 채집된 시료들은 SMV, SYMMV, SYCMV, SbDV, PSV, BCMV, AMV, PEMV, CMV, CCMV, TSV, BBWV2, BYMV와 CPMV 14종에 대한 정밀 진단을 위하여 각각의 종특이 프라이머를 이용하여 RT-PCR 진단이 수행되었다. 그 결과 채집된 시료의 86%가 바이러스에 감염된 것으로 확인 되었으며, SMV 141, SYMMV 14, PSV 8과 SYCMV 5점이 확인되었다. 대구와 나주에서 채집된 2점의 시료들에서는 SYMMV와 SYCMV가 복합감염 되어있었다. 나머지 미동정 시료에 대한 정밀 분석과 지속적인 발생상황 조사가 이루어 져야 할 것이다. It had been reported that soybean viral diseases are Soybean mosaic virus (SMV), Soybean dwarf virus (SbDV), Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV), Cowpea mosaic virus (CPMV), Soybeun yellow rnottle mosaic virus (SYMMV), Soybean yellow common mosaic virus (SYCMV) and Peanut stunt virus (PSV) in Korea. Among these viral diseases, SMV caused severe damage to soybean in Korea, Although SMV occurred more than 90% in the past, recently several viruses such as SYMMV, SYCMV, SbDV and PSV have been reported in Korea, It means that the incidence of viral diseases are changing in soybean. To identify the viruses infecting soybean in Korea, the 193 samples with viral symptoms were collected in 19 areas of 8 provinces. And then the RT-PCR assay was conducted to detect 14 different viruses such as SMV, SYMMV, SYCMV, SbDV, PSV, BCMV, AMV, PEMV, CMV, CCMV, TSV, BBWV2, BYMV and CPMV. The results indicated that about 86% of samples were identified as Virus-infected, Among 193 soybean samples, 141 SMV, 8 PSV, 14 SYMMV, 5 SYCMV were detected. Two samples were coinfected with SYMMV and SYCMV. The rest of them were likely to express the mosaic similar to virus-induced symptoms. Therefore, the identifications of the unknown samples have been performed by the dererminatlon of the nucleotide sequences of the genomic RNAs.

Reverse transcription Loop-mediated isothermal amplification을이용한 Soybean mosaic virus의 진단

이영훈,배대현,김봉섭,윤영남,배순도,김현주,Bishwo P. Mainali,박인희,이수헌,강항원 한국식물병리학회 2015 식물병연구 Vol.21 No.4

Soybean mosaic virus (SMV) is a prevalent pathogen that causes significant yield reduction in soybean production worldwide. SMV belongs to potyvirus and causes typical symptoms such as mild mosaic, mosaic and necrosis. SMV is seed-borne and also transmitted by aphid. Eleven SMV strains, G1 to G7, G5H, G6H, G7H, and G7a were reported in soybean varieties in Korea. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SML-F3/B3/FIP/BIP from coat protein gene sequence of SMV. After the reaction of RT-LAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I under daylight and UV light. Optimal reaction condition was at 58℃ for 60 min and the primers of RT-LAMP showed the specificity for nine SMV strains tested in this study. Soybean mosaic virus(SMV)는 potyvirus 속에 속하며, 모자이크, 괴사, 기형 등의 병징을 야기하고 국내에서는 11개 계통(G1 to G7, G5H, G6H, G7H, G7a)이 보고되어있다. Reverse transcription loop-mediated isothermal amplification(RT-LAMP) 방법은 등온에서 유전자 증폭이 가능하게 하며, 이 방법은 PCR 과정이나 전기영동 없이도 바이러스에 감염된 식물을 검출할수 있는 이점이 있다. RT-LAMP의 최적반응 조건은 58℃, 60분으로 확인되었다. 특이성 검정을 위해 콩에서 발생하는 여러 바이러스들과 보유중인 SMV의 9 계통에서 그 특이성을 확인하였다. 그 결과 SMV에 대한 RT-LAMP primer들의 종 특이성이 확인되었으며, SMV의 계통들에 대해서도 적용이 가능한 것으로 확인되었다. 항온수조와 heating block과 같은 간편한 등온 장치에서 재현성을 확인하기 위해 Thermocycler 기기와 비교하여증폭 여부를 확인한 결과 반응의 차이는 나타나지 않았다. RTLAMP 반응 이후, 반응물을 전기영동과 SYBR Green I을 이용하여 자연광과 UV광에서 증폭 여부를 확인하였다. 그 결과 전기영동, 자연광, portable UV light와 UV transilluminator에서 모두 반응이 확인되었다.

Complete Genome Sequences of the Genomic RNA of Soybean mosaic virus Strains G7B and G5

Kim, Kook-Hyung,Lim, Won-Seok,Kim, Yul-Ho The Korean Society of Plant Pathology 2003 Plant Pathology Journal Vol.19 No.3

The complete nucleotide sequences of the genomic RNAs of Soybean mosaic virus strains GS (SMV-G5) and G7H (SMV-G7H) were determined and compared with sequences of other SMV strains. Each viral RNA was determined to be 9588 nucleotides in length excluding the poly (A) tail and contained an open reading frame to encode a polyprotein subsequently processed into up to ten proteins by proteolytic cleavage. Com-parison of the amino acid sequences with those of other SMV strains showed high percentage of amino acid sequence homology with the same genome organization. The nucleotide and the deduced amino acid sequences between SMV-G5 and SMV-G7H were greater than 99% identity. When compared with those of other SMV strains in a phylogenetic analysis of the nucleotide and deduced amino acid sequences, they formed a distinct virus clade showing over 97% amino acid identity, but were more distantly related to the other potyvirus (44.1-69.6% identity). Interestingly, SMV G7H strain caused a severe mosaic or necrosis symptom in soybean cultivars including Jinpum-1, Jinpum-2, and Sodam, whereas, no symptom was observed in SMV-G5 inoculation. Complete nucleotide sequences of these strains will give clues for determining symptom determinant(s) in future research.

Complete Genome Sequences of the Genomic RNA of Soybean mosaic virus Stranins G7H and G5

임원석,김국형,김유호 한국식물병리학회 2003 Plant Pathology Journal Vol.19 No.3

The complete nucleotide sequences of the genomic RNAs of Soybean mosaic virus strains G5 (SMV-G5) and G7H (SMV-G7H) were determined and compared with sequences of other SMV strains. Each viral RNA was determined to be 9588 nucleotides in length excluding the poly (A) tail and contained an open reading frame to encode a polyprotein subsequently processed into up to ten proteins by proteolytic cleavage. Comparison of the amino acid sequences with those of other SMV strains showed high percentage of amino acid sequence homology with the same genome organization. The nucleotide and the deduced amino acid sequences between SMV-G5 and SMV-G7H were greater than 99% identity. When compared with those of other SMV strains in a phylogenetic analysis of the nucleotide and deduced amino acid sequences, they formed a distinct virus clade showing over 97% amino acid identity, but were more distantly related to the other potyvirus (44.1- 69.6% identity). Interestingly, SMV G7H strain caused a severe mosaic or necrosis symptom in soybean cultivars including Jinpum-1, Jinpum-2, and Sodam, whereas, no symptom was observed in SMV-G5 inoculation. Complete nucleotide sequences of these strains will give clues for determining symptom determinant(s) in future research.