돼지 도축폐수 처리를 위한 RABC 공정의 생물막 세균군집 구조

성기문,이동근,박성주,Sung, Gi-Moon,Lee, Dong-Geun,Park, Seong-Joo 한국미생물학회 2011 미생물학회지 Vol.47 No.1

돼지 도축폐수를 처리하는 Rotating Activated Bacillus Contactors (RABC) 공정의 세균군집 특성을 파악하기 위하여 그람양성세균수와 총세균수를 계수하여 기존 고도폐수처리공정인 A2O (Anaerobic-Anoxic-Oxic) 공정과 비교하였다. RABC 공정의 생물막 세균군집구조는 비배양기법인 16S rDNA 염기서열결정법을 이용하여 분석하였다. RABC 공정의 총세균수에 대한 그람양성세균수 비율은 생물막(32%)에 비해 최종포기조(1282%) 및 반송슬러지(958%)에서 현저히 증가한 반면, A2O 공정의 그람양성세균수 비율은 호기조(40%)와 반송슬러지(49%) 모두에서 상대적으로 훨씬 낮았다. 총 9개 문에 해당하는 92개의 클론이 검출되었으며, 이 가운데 최우점 집단은 Proteobacteria (64.1%)와 Actinobacteria (18.4)%로서 이들 2개 문이 전체의 82.5%를 차지하였다. 3번째로 많이 검출된 것은 내생포자형성세균집단이 속하는 Firmicutes (5.4%) 문이었다. 소량 검출된 나머지 6개 문은 Bacteroidetes (3.3%), Chlorobi (2.2%), Nitrospirae (1.1%), Chlorofleix (1.1%), Acidobacteria (1.1%), Fusobacteria (1.1%)의 순이었다. Proteobacteria 문 중에서는 Betaproteobacteria 강 34.8%, Alphaproteobacteria 강 26.1%로서 대부분을 차지하였고, Gammaproteobacteria 강은 3.2%이었다. 내생포자형성세균집단의 비율은 모두 19.4%로서, Firmicutes 문 5.4%와 Actinobacteria 문의 Intrasporangiaceae과 14.0%이었다. 질화세균 및 탈질세균과 관련된 클론 비율 6.5%, 인축적세균과 관련된 클론 비율 5.4%를 기록함으로써 무기영양소 및 악취 제거능력을 가진 세균집단이 RABC 생물막에 많이 서식하고 있음이 확인되었다. Culture-independent microscopic observations and 16S rDNA analyses were applied to describe the bacterial community inherent to the biofilm structure of the RABC (Rotating Activated Bacillus Contactors) process for swine butchery wastewater treatment. The ratios of Gram-positive bacterial counts to total bacterial counts of the RABC process were significantly increased in the last aeration tank as well as returned sludge, while those of the existing A2O (Anaerobic-Anoxic-Oxic) process maintained constant from aeration tanks to returned sludge. Totally nine phyla were recovered by 16S rDNA analysis, two of which were major groups: the Proteobacteria (64.1%) and the Actinobacteria (18.4%). The third major group was the endospore-forming Firmicutes (5.4%). The remaining six minor groups are the Bacteroidetes (3.3%), the Chlorobi (2.2%), the Nitrospirae (1.1%), the Chlorofleix (1.1%), the Acidobacteria (1.1%), and the Fusobacteria (1.1%). The ratio of endospore-forming bacteria was 19.4%, which was composed of the members of the Firmicutes phylum (5.4%) and the Intrasporangiaceae family (14.0%) of the Actinobacteria phylum. Nitrifying and denitrifying related- and phosphorus accumulating related-sequences were composed of 6.5% and 5.4% of total community, respectively, these could mean the high capacity of the RABC process to remove odor compounds and reduce eutrophication by efficient removing inorganic nutrients.

수정 RABC 공정을 이용한 영양염류 제거능 제고에 관한 연구

김선희,김동환,장기웅,김응호 대한상하수도학회 2016 상하수도학회지 Vol.30 No.1

This study was performed to develop a new process technology for advanced wastewater treatment using a modified Rotating Activated Bacillus Contactor (RABC) process that adopts anoxic-oxic suspended biomass tanks to enhance nutrients removal. A modified lab-scale RABC process was applied to examine its applicability and to obtain the design factors for the optimum operation of the system. The modified RABC process showed a little more stable and high nutrients removal efficiency than the prototype RABC process: about 70% of nitrogen and 55% of phosphorous removal when the low organic loading (influent COD 200mg/L). However, the processing efficiency of nutrients removal rates was enhanced to great extent when high organic loading: nitrogen 90% and phosphorous 85% (influent COD 500mg/L). High organic loading stimulated extremely good biomass attachment on the reticular carrier RABC stage and the excellent nutrients removal, nevertheless with almost no offensive odor.

망상형 회전식 바실러스 접촉장치를 이용한 하수의 고도처리공정에 관한 연구

김응호 ( Kim Eung Ho ),조연제 ( Jo Yeon Je ),박성주 ( Park Seong Ju ),신광수 ( Sin Gwang Su ),임수빈 ( Im Su Bin ),정진권 ( Jeong Jin Gwon ) 한국물환경학회 2004 한국물환경학회지 Vol.20 No.2

A new technology for advanced wastewater treatment was developed using a modified Rotating Biological Contactor (RBC) process, named as Rotating Activated Bacillus Contactor (RABC) process that utilizes Bacillus sp., the facultatively anaerobic or activated microaerophilic bacteria on multiple-stage reticular rotating carriers, as a predominant species. The RABC process for a municipal wastewater with relatively low concentrations of organics, nitrogen, and phosphorus showed stable and high removal efficiencies, less than BOD_(5) 10 ㎎/L, T-N 15 ㎎/L, and T-P 1.5 ㎎/L in final effluent. The performance load of RABC process was shown to be 1.23 ㎏·BOD/㎡·day for the first stage (average 0.31 ㎏·BOD/㎡·day for the total stages) based on both removed BOD and converted disc area corresponding to the reticular one. The sludge produced in the RABC process is characterized by low generation rate (about 0.18㎏·MLSS/㎏·BOD) and excellent settleability. The number ratio of Bacillus (2.4×10(6)) CFU/ml) to heterotrophic bacteria ( 3.6×10_(7) CFU/ml) inhabiting in the biofilms of the RABC process was 6.7%, indicating that Bacillus sp. was a predominant species in the biofilms. The RABC process with reticular rotating carriers showed its excellent performance for the advanced wastewater treatment without any offensive odor problem due to organic overloading.

회전식 부착 바실러스를 이용한 하수고도처리 공정에서의 총대장균군 제거 특성

김응호 ( Eung Ho Kim ),조연제 ( Yeon Je Cho ),박성주 ( Seong Joo Park ),신광수 ( Kwang Soo Shin ),임수빈 ( Soo Bin Yim ),박현주 ( Hyun Ju Park ) 한국물환경학회 2005 한국물환경학회지 Vol.21 No.1

This study was performed to examine the disinfection capability of a Rotating Activated Bacillus Contactor (RABC) system, in which the predominant species, Bacillus sp. was expected to have a removal or inactivation effect of total coliforms. In a settling test with mixtures of E. coli and Bacillus sp., a high removal of E. coli was observed at 20~40℃, while insignificant removal at 10℃. In a batch test, a 4.5% addition of Bacillus sp. to activated sludge considerably enhanced the removal effect of total coliforms, indicating Bacillus sp. played an important role in improving the settlability of the sludge and coliforms. In a pilot scale RABC system, the concentration of total colifroms reduced remarkably in the settling tank, suggesting that total coliforms in the RABC process were eliminated through coagulation and precipitation, probably due to extracellular polymeric substance (EPS) of Bacillus sp. The fraction of Bacillus sp. in the total cell count in the RABC process was in the range of 4.5%~6.3%. The majority (75%) of the Bacillus sp. in the RABC process was Bacillus subtilis which is known to enhance coagulation and precipitation by producing EPS. Hence, an adoption of a RABC process might be able to eliminate the disinfection unit process from a wastewater treatment system.

Pilot-scale Study on Nitrogen Removal of Effluent from Biogas Plant

유성인(Yoo, Sungin),유영섭(Yu, Youngseob),이용세(Lee, Yongsei),박현수(Park, Hyunsu),유희찬(Yoo, Heechan) 한국신재생에너지학회 2011 한국신재생에너지학회 학술대회논문집 Vol.2011 No.11

A rotating activated bacillus contactor (RABC) process with a series of aerobic reactors was tested in pilot scale to treat digested liquid from an anaerobic digester treating swine wastewater and sewage sludge. The influent (digested liquid) for the RABC process showed C/N ratios less than 2 as a typical feature of effluent from anaerobic digesters. The pilot process, which consists of three 3 RABC reactors, four aerobic tanks and a sedimentation tank, was operated for 210 days with a hydraulic retention time of 20 days without pH and temperature control. Since the Bacillus-enriched aerobic reactors shows high efficiencies of nitrogen removal at low DO levels less than 1.0 mg/L, they were operated at reduced aeration intensities. With relatively low concentrations of organics in comparison with nitrogen concentrations, the RABC process tested in this study showed stable and high nitrogen and organics removal efficiencies over 80%. The nitrogen removal process tested in this study was proven to be an effective and operation-cost saving (lower aeration) method to remove nitrogen without adding external carbon sources to meet the optimum C/N ratio.

Ranking Artificial Bee Colony for Design of Wireless Sensor Network

김성수(Sung-Soo Kim 한국산업경영시스템학회 2019 한국산업경영시스템학회지 Vol.42 No.1

A wireless sensor network is emerging technology and intelligent wireless communication paradigm that is dynamically aware of its surrounding environment. It is also able to respond to it in order to achieve reliable and efficient communication. The dynamical cognition capability and environmental adaptability rely on organizing dynamical networks effectively. However, optimally clustering the cognitive wireless sensor networks is an NP-complete problem. The objective of this paper is to develop an optimal sensor network design for maximizing the performance. This proposed Ranking Artificial Bee Colony (RABC) is developed based on Artificial Bee Colony (ABC) with ranking strategy. The ranking strategy can make the much better solutions by combining the best solutions so far and add these solutions in the solution population when applying ABC. RABC is designed to adapt to topological changes to any network graph in a time. We can minimize the total energy dissipation of sensors to prolong the lifetime of a network to balance the energy consumption of all nodes with robust optimal solution. Simulation results show that the performance of our proposed RABC is better than those of previous methods (LEACH, LEACH-C, and etc.) in wireless sensor networks. Our proposed method is the best for the 100 node-network example when the Sink node is centrally located.

식품폐수처리 RABC system의 생물막과 포기조 대사지문 비교

이동근(Dong-Geun Lee),유기환(Ki-Hwan Yoo),성기문(Gi Moon Sung),박성주(Seong Joo Park),이재화(Jae-Hwa Lee),하배진(Bae Jin Ha),하종명(Jong-Myung Ha),이상현(Sang-Hyeon Lee) 한국생명과학회 2009 생명과학회지 Vol.19 No.3

돈ㆍ도축 식품폐수를 처리하는 생물막 시스템인 RABC(rotating activated Bacillus contactor) 공정의 생물막과 점감식으로 운영되는 포기조의 동일한 시료를 대상으로 Biolog GN2 plate를 이용한 호기/혐기 대사지문 분석으로 다음과 같은 결과를 얻었다. 첫째 대응표본 상관관계 분석과 pairs t-test를 통하여 매일 측정값보다 일별변화량이 전반적인 군집구조의 유사성을 나타내는 것으로 판단되었다. 둘째 생물막이 포기조 시료들에 비해 높은 탄소원 이용도를 보여(p<0.01) 생물막의 높은 유기물 제거 능력을 알 수 있었고 포기조 시료사이는 이용된 탄소원의 상관관계가 높았고(78%) 활성정도도 비슷하였다(p=0.287). 셋째 활발하게 이용된 탄소원의 종류는 시료별로 차이를 보여 활성을 보이는 군집이 각 처리단계 별로 변화하는 것으로 판단되었다. 마지막으로 같은 시료라도 호기/혐기 조건에 따라 대사지문에 차이를 보여 미호기성 및 혐기성 군집구성원에 의한 활성 차이를 확인하였다. 향후 대사지문(metabolic fingerprinting)을 통한 활성군집 비교에 일별변화량과 혐기조건 분석을 병행하면 더욱 풍부한 분석이 가능할 것이다. Metabolic fingerprinting of microbial communities was investigated with Biolog GN2 plates using samples of biofilm and aeration tanks from an RABC (rotating activated Bacillus contactor) system - an advanced wastewater treatment system for the food wastewater of pig slaughterhouses. Aerobic and anaerobic results revealed the following four aspects. First, simple matching and pairs t-test of daily variation showed more defined qualitative and quantitative relatedness of active microbial communities than that of mere optical densities. Second, metabolic potentials were higher in biofilm than in aeration tanks (p<0.01), meaning higher activity of biofilm. Third, two aeration tanks showed the highest similarity (78%) and similar metabolic power (p=0.287). However, actively used carbon sources were different among samples, signifying change of active communities at each wastewater treatment step. Finally, aerobic and anaerobic metabolic fingerprinting patterns were different for the same samples representing activities of microaerophilic and/or anaerobic communities. These results suggest that daily variation and anaerobic incubation would help in the comparison of metabolic fingerprintings.

Dominance of Endospore-forming Bacteria on a Rotating Activated Bacillus Contactor Biofilm for Advanced Wastewater Treatment

Park, Seong-Joo,Yoon, Jerng-Chang,Shin, Kwang-Soo,Kim, Eung-Ho,Yim, Soo-Bin,Cho, Yeon-Je,Sung, Gi-Moon,Lee, Dong-Geun,Kim, Seung-Bum,Lee, Dong-Uk,Woo, Sung-Hoon,Koopman, Ben The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.2

The bacterial diversity inherent to the biofilm community structure of a modified rotating biological contactor wastewater treatment process, referred to as the Rotating Activated Bacillus Contactor (RABC) process, was characterized in this study, via both culture-dependent and culture-independent methods. On the basis of culture-dependent methods, Bacillus sp. were found to exist in large numbers on the biofilm (6.5% of the heterotrophic bacteria) and the microbial composition of the biofilms was quite simple. Only three phyla were identified-namely, the Proteobacteria, the Actinobacteria (High G+C Gram-positive bacteria), and the Firmicutes (Low G+C Gram-positive bacteria). The culture-independent partial 16S rDNA sequence analysis revealed a considerably more diverse microbial composition within the biofilms. A total of eight phyla were recovered in this case, three of which were major groups: the Firmicutes (43.9%), the Proteobacteria (28.6%), and the Bacteroidetes (17.6%). The remaining five phyla were minor groups: the Planctomycetes (4.4%), the Chlorobi (2.2%), the Actinobacteria (1.1%), the Nitrospirae (1.1%), and the Verrucomicrobia (1.1%). The two most abundant genera detected were the endospore-forming bacteria (31.8%), Clostridium and Bacillus, both of which are members of the Firmicutes phylum. This finding indicates that these endospore-forming bacteria successfully colonized and dominated the RABC process biofilms. Many of the colonies or clones recovered from the biofilms evidenced significantly high homology in the 16S rDNA sequences of bacteria stored in databases associated with advanced wastewater treatment capabilities, including nitrification and denitrification, phosphorus accumulation, the removal of volatile odors, and the removal of chlorohydrocarbons or heavy metals. The microbial community structures observed in the biofilms were found to correlate nicely with the enhanced performance of advanced wastewater treatment protocols.

식품폐수 처리 단계별 미생물 대사지문

유기환,이상현,이동근,Yoo, Ki-Hwan,Lee, Sang-Hyeon,Lee, Dong-Geun 한국환경보건학회 2008 한국환경보건학회지 Vol.34 No.4

To determine structure and activities of microbial communities in a food wastewater treatment system, biofilm of RABC (rotating activated Bacillus contactor) and samples of aeration tanks were analyzed. Heterotrophic bacterial concentrations were similar between biofilm and stage 1 aeration tank and decreased 2-log at stage 3 aeration tank as dissolved oxygen decreased, however portions of Bacillus groups were increased at stage 3 aeration tank. It was revealed by quantitative and qualitative analysis of metabolic fingerprinting patterns of Biolog GN2 plate that RABC represented much higher activities and a different microbial community structure compared to aeration tanks. Metabolic fingerprinting showed the carbon sources that isolated Bacillus groups could or could not use, were used similarly meaning that not only Bacillus groups but also other microbial groups would contribute to the treatment of wastewater.

Metabolic Potentials of Biofilm and Aeration Tanks of RABC System

Dong-Geun Lee,Ki-Hwan Yoo,Gi Moon Sung,Yeon-Je Cho,Seong Joo Park,Jae-Hwa Lee,Bae Jin Ha,Jong-Myung Ha,Sang-Hyeon Lee 한국생명과학회 2008 한국생명과학회 심포지움 Vol.49 No.-