In vitro Effects of Mussel-Inspired Polydopamine Coating on Ti6Al4V Alloy

( Joon Ho Lee ),( Young Wook Lim ),( Soon Yong Kwon ),( Yong Sik Kim ) 한국조직공학·재생의학회 2013 조직공학과 재생의학 Vol.10 No.5

Mussel adhesion phenomena in nature have inspired the integration of inorganic hydroxyapatite (HA) crystals within versatile materials. One example is the simple, aqueous, two-step functionalization approach, called polydopamine-assisted hydroxyapatite formation (pHAF), which consists of the chemical activation of material surfaces via polydopamine coating and the growth of hydroxyapatite in a simulated body fluid (SBF). For this study, we anticipated that such a polydopamine coating on the surface of titanium (Ti) alloy would improve the ability of cementless stems to osseointegrate. We compared the in vitro ability of cells to adhere to polydopamine-coated Ti alloy and machined Ti alloy. We performed energy-dispersive x-ray spectroscopy (EDS) and scanning electron microscopy (SEM) investigations to assess the structure and morphology of the surfaces. Biological and morphological responses to osteoblast cell lines (MC3T-E1) were then examined by measuring cell proliferation, cell differentiation (alkaline phosphatase (ALP) activity), and actin filament formation. Real-time polymerase chain reaction (PCR) was used to analyze gene expression for osteocalcin, osteonectin, and osteoprotegerin. Cell proliferation and ALP activity in the polydopamine-coated Ti alloy did not differ statistically compared to the other group. The polydopamine-coated Ti alloy exhibited better apatite formation ability than the untreated alloy, as evidenced by apatite formation after SBF immersion for 10 days. Molecular biological analysis did not differ statistically between the groups. The surface modification of the Ti alloy by coating with polydopamine did not change the biological properties of the Ti alloy. This may make some difficulties for osteogenesis signaling for the cells.

폴리도파민 표면화학: 발명 10 년의 이야기

엄수민 ( Soomin Eom ),박홍기 ( Hong Key Park ),박지효 ( Jihyo Park ),홍선기 ( Seonki Hong ),이해신 ( Haeshin Lee ) 한국접착및계면학회 2018 접착 및 계면 Vol.19 No.1

바다에서 서식하는 홍합의 독특한 수중 접착성을 모방하여 개발된 폴리도파민 (polydopamine)코팅 기술은 2007년 처음 발표된 이래 지난 10년 동안 전세계적으로 매우 크게 발전하였다. 표면 비특이적인 코팅 능력을 통해 이제까지 표면 개질이 어려웠던 다양한 표면을 제한 없이 기능화 할 수 있는 유일한 표면 화학으로 자리 잡았으며, 또한 다양한 반응 조건에서의 코팅 방법이 새롭게 보고되면서, 산업 전반에 걸친 폴리도파민의 응용 범위가 기하급수적으로 넓어지고 있다. 한편, 밝혀지지 않은 폴리도파민의 복잡한 화학적 구조와 형성 반응 메커니즘에 관한 재료화학적 기초 연구도 지속적으로 보고되고 있으며, 폴리도파민의 전구체인 도파민 (dopamine)과 유사한 분자 구조를 가지는 다양한 카테콜아민 (catecholamine) 화합물과 폴리페놀 (polyphenol)의 표면 코팅 능력이 새로이 밝혀지고 있다. 본 연구에서는, 지난 10년 동안 전세계적으로 급속한 발전을 이룬 폴리도파민의 특성 및 응용분야에 대해 살펴보고, 이를 통해 폴리도파민의 표면 화학 분야에서의 의의와 가능성에 대해 논의하고자 한다. Polydopamine coating is one of the most straightforward and widely used method for surface modification inspired by adhesiveness of mussel foot protein contributed by co-existence of catechol and amine. This technique has been utilized not only in surface modification but other numerous fields of study as well. For the past decade, the subject of polydopamine has been thoroughly studied since the initial polydopamine research published in 2007, including its chemical structure, coating conditions, and material characteristics. In this study, we report the current trends and progress of polydopamine coating methods, the newly developing areas of polydopamine related research such as using dopamine derivatives and polyphenolic compounds, improvement of various functionalization and application of polydopamine coating, and explain the state of current attempts to discover the chemical mechanism, structure, and properties of polydopamine.

Efficacy of polydopamine-coated titanium in order to improve bond strengths for dental resin cement

김현진,권태엽 대한치과재료학회 2017 대한치과재료학회지 Vol.44 No.2

This study investigated the effects polydopamine-coated titanium surfaces on resin cement shear bond strengths, and characterized polydopamine-coated titanium surfaces. Polydopamine was coated onto the titanium surfaces by immersion in a 2 mg/mL dopamine solution in 10 mM Tris-HCl buffer at pH 8.5. The surface characteristics of polydopamine-coated titanium plates were observed by X-ray photoelectron spectroscopy (XPS), scanning electron microscope (SEM) and contact angle measurements. And then, the resin cement bond strengths were evaluated under shear bond strength mode. The formation of polydopamine coating on titanium has been confirmed by XPS analyses of the materials surfaces. The absence of Ti2p peak from polydopamine-coated titanium surface indicates that the polydopamine coating completely has covered the titanium surface. Microstructural features revealed that nanometer-sized bright granules were randomly distributed after coating. The cement shear bond strength of polydopamine-coated titanium plate was higher than the non-coated titanium plates (p<0.05). The more surface wetted with the cement material, the higher the resultant shear bond strength value. As a results, bond strengths are correlated to wettability. These results suggest that a treatment of polydopamine surface provides an effective surface condition to enhance the cement bond strength to titanium.

ZrIV complexation for stability enhancement of polydopamine coatings and rapid grafting of amine compounds

이아리수,김인호,강성민 대한화학회 2023 Bulletin of the Korean Chemical Society Vol.44 No.11

Polydopamine is extensively researched in multiple disciplines due to its ability to coat various solid substrates and undergo secondary functionalization. However, a drawback of polydopamine is its tendency to degrade under strong alkaline conditions. This study aims to address this limitation. Specifically, it is observed that the stability of polydopamine significantly increases when it forms a complex with zirconium (IV). Furthermore, the polydopamine/ zirconium (IV) complex demonstrates efficient incorporation of amine compounds, opening up possibilities for further applications.

아크릴산이 그라프트된 나노섬유에서의 폴리도파민 코팅

신영민,김우진,박종석,권희정,노영창,임윤묵 한국방사선산업학회 2011 방사선산업학회지 Vol.5 No.4

The surface property of the materials used in tissue engineering application has beenessential to regulate cellular behaviors by directing their adhesion on the materials. To modulatesurface property of the synthetic biodegradable materials, a variety of surface modification techniqueshave used to introduced surface functional groups or bioactive molecules, recently polydopaminecoating method have been introduce as a facile modification method which can be coatedon various materials such as polymers, metals, and ceramics regardless of their surface property. However, there are no reports about the degree of polydopamine coating on the materials withdifferent hydrophilicity. In the present study, we prepared acrylic acid grafted nanofibrous meshesusing electron-beam irradiation, and then coated meshes with polydopamine. Polydopamine successfullycoated on the all meshes, both properties of acrylic acid and polydopamine were detectedon the meshes. In addition, the degree of polydopamine deposition on the materials has been alteredaccording to surface hydrophilicity, which was approximately 8-times greater than those on thenon-modified materials. In conclusion, dual effect from the acrylic acid grafting and polydopaminemay give a chance as a alternative tool in tissue engineering application.

Effective Immobilization of BMP-2 Mediated by Polydopamine Coating on Biodegradable Nanofibers for Enhanced in Vivo Bone Formation

Cho, Hyeong-jin,Madhurakkat Perikamana, Sajeesh Kumar,Lee, Ji-hye,Lee, Jinkyu,Lee, Kyung-Mi,Shin, Choongsoo S.,Shin, Heungsoo American Chemical Society 2014 ACS APPLIED MATERIALS & INTERFACES Vol.6 No.14

<P>Although bone morphogenic proteins (BMPs) have been widely used for bone regeneration, the ideal delivery system with optimized dose and minimized side effects is still active area of research. In this study, we developed bone morphogenetic protein-2(BMP-2) immobilized poly(<SMALL>l</SMALL>-lactide) (PLLA) nanofibers inspired by polydopamine, which could be ultimately used as membranes for guided bone regeneration, and investigated their effect on guidance of in vitro cell behavior and in vivo bone formation. Surface chemical analysis of the nanofibers confirmed successful immobilization of BMP-2 mediated by polydopamine, and about 90% of BMP-2 was stably retained on the nanofiber surface for at least 28 days. The alkaline phosphatase activity and calcium mineralization of human mesenchymal stem cells (hMSCs) after 14 days of in vitro culture was significantly enhanced on nanofibers immobilized with BMP-2. More importantly, BMP-2 at a relatively small dose was highly active following implantation to the critical-sized defect in the cranium of mice; radiographic analysis demonstrated that 77.8 ± 11.7% of newly formed bone was filled within the defect for a BMP-2-immobilized groups at the concentration of 124 ± 9 ng/cm<SUP>2</SUP>, as compared to 5.9 ± 1.0 and 34.1 ± 5.5% recovery, for a defect-only and a polydopamine-only group, respectively. Scanning and transmission electron microscopy of samples from the BMP-2 immobilized group showed fibroblasts and osteoblasts with nanofiber strands in the middle of regenerated bone tissue, revealing the importance of interaction between implanted nanofibers and the neighboring extracellular environment. Taken together, our data support that the presentation of BMP-2 on the surface of nanofibers as immobilized by utilizing polydopamine chemistry may be an effective method to direct bone growth at relatively low local concentration.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2014/aamick.2014.6.issue-14/am501391z/production/images/medium/am-2014-01391z_0011.gif'></P>

Polydopamine-coated nanocomposites of <i>Angelica gigas</i> Nakai extract and their therapeutic potential for triple-negative breast cancer cells

Nam, Suyeong,Lee, Song Yi,Kim, Jung-Jin,Kang, Wie-Soo,Yoon, In-Soo,Cho, Hyun-Jong Elsevier 2018 Colloids and Surfaces B Vol.165 No.-

<P><B>Abstract</B></P> <P>Polydopamine (PD)-coated nanocomposites (NCs) based on the ethanol extract of <I>Angelica gigas</I> Nakai (AGN EtOH ext) were fabricated and evaluated for breast cancer therapy. AGN NCs were prepared using a modified emulsification-solvent evaporation method and were further incubated in dopamine solution (at pH 8.6) to be covered with the PD layer. PD-AGN NCs with a 213-nm mean diameter, narrow size distribution, and negative zeta potential values were fabricated in this study. Less negative (close to zero) zeta potential value of PD-AGN NCs than that of AGN NCs implied the existence of the PD layer in the outer surface of NCs. The PD layer in PD-AGN NCs was also identified by X-ray photoelectron spectroscopy (XPS) and ultraviolet (UV)/visible absorption analyses. The sustained release of decursin (D) and decursinol angelate (DA), as major active pharmacological components of AGN, was observed in both AGN NCs and PD-AGN NCs. Enhanced cellular binding property of PD-AGN NCs, compared to AGN NCs, in MDA-MB-231 (human breast adenocarcinoma; triple-negative breast cancer) cells was observed. Improved anticancer activities of PD-AGN NCs compared with those of AGN EtOH ext and AGN NCs were also shown in MDA-MB-231 cells. The developed PD-AGN NCs may be used as remarkable platform nanocarriers for efficient breast cancer therapy.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Nanocomposites (NCs) composed of <I>Angelica gigas</I> Nakai (AGN) extract were fabricated. </LI> <LI> Polydopamine (PD) layer was coated onto the outer surface of AGN NCs. </LI> <LI> Cellular adhesiveness of PD-AGN NCs was higher than AGN NCs in breast cancer cells. </LI> <LI> Improved anticancer activities were shown in PD-AGN NCs group than AGN NCs group. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Potential and performance of a polydopamine-coated multiwalled carbon nanotube/polysulfone nanocomposite membrane for ultrafiltration application

Merry Sianipar,김승현,민충식,Leonard D. Tijing,손호경 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.34 No.-

The addition of multiwalled carbon nanotubes (MWNTs) as inorganic fillers is well known to improvemembrane performance for water desalination. Most MWNTs are treated by acid treatment to enhancetheir hydrophilicity before their applications in membranes. However, acid treatment leads to structuraldamages of the MWNT wall. An alternative way of improving the hydrophilicity of MWNTs is throughcoating of polydopamine (Pdop), where MWNT wall damage is avoided. In the present study,polydopamine-coating on MWNT is carried out at pH 8.5 and at room temperature (23–25 8C). Differentconcentrations (0.1–0.5 wt%) of Pdop-MWNTs were incorporated into polysulfone (Psf) membranesfabricated by phase inversion. The results showed that the incorporation of Pdop-coated MWNTs hasincreased the membrane permeability using BSA solution (1000 ppm) by 19–50% depending on theamount of Pdop-MWNTs in the membrane, and has maintained good rejection performances (99.88%). Moreover, the antifouling properties of the nanocomposite membranes were also improved. Here, theoptimum dose was determined to be 0.1 wt% of Pdop-MWNTs. Furthermore, even though the Pdop-MWNT/Psf membranes showed lower permeability than acid-MWNT/Psf membrane, the Pdop-MWNT/Psf membrane obtained higher mechanical strength and would be potentially sustainable for a long termultrafiltration operation.

Zwitterion/Polydopamine-coated separator for high ion conductivity in Li-ion battery and efficient polysulfide barrier in Li-S battery

이준혁,유필진 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1

Recently, various studies related to adding functionalities to battery separators have been reported. In particular, high conductivity of separators is considered to be one of the crucial properties for fast charge/discharge characteristics in battery applications. Here, we attached sulfobetaine zwitterionic functional group on polydopamine- coated polyolefin separator to induce dissociation lithium-ion cation and counter anion for high ion conductivity. Furthermore, zwitterion/polydopamine composite-coated separator showed prohibition effect for “polysulfide shuttling” in a lithium-sulfur battery system.

Polydopamine/β-cyclodextrin/coal fly ash composite for the highly efficient extraction of uranium from water environment

Yan Wang,Yong Zhang,Xiaolin Liu,Sen Sun,Shiyi Qin,Jiaqi Huang,Bowei Chen 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.127 No.-

To obtain a green, low-cost and efficient adsorbent, polydopamine (PDA) and b-cyclodextrin (b-CD) wereadopted to modify coal fly ash (CFA) to prepare polydopamine/b-cyclodextrin/coal fly ash composite(PDA/b-CD/CFA). The successful introduction of PDA and b-CD was proved by FT-IR, XRD and XPS. Theuranium extraction efficiency on PDA/b-CD/CFA reached 95.6% (pH = 5.0, T = 298 K, C0 = 10 mg/L andm/V = 0.2 g/L) and the whole adsorption process was perfectly fitted by the Pseudo-second-order model(R2 = 0.999), illustrating that uranium was extracted via chemisorption. The correlation coefficient R2 ofLangmuir model was 0.999, which was higher than other models, meaning that uranium extractionbehavior on PDA/b-CD/CFA was uniform monolayer adsorption. The maximum extraction capacity of uraniumon PDA/b-CD/CFA calculated by Langmuir model was 537.6 mg/g, which was larger than most ofreported adsorbents, indicating that PDA/b-CD/CFA was a potential candidate for uranium extractionfrom water environment. Moreover, PDA/b-CD/CFA performed excellent uranium extraction propertieswith the existence of coexisting ions and the desorption efficiency of uranium by PDA/b-CD/CFA washigher to 95.8% at the fifth cycles, fully suggesting that PDA/b-CD/CFA possessed good selectivity andcycle stability. Characterization results demonstrated that uranium was immobilized on PDA/b-CD/CFAthrough chelation, complexing action, electrostatic interaction and hydrogen bonding.