Phellinus linteus, Phellinus baumii 및 Phellinus gilvus의 형태, 일반성분, 원소분석 및 무기성분 함량의 비교

배재성,장광호,이만휘,정규식,조우식,최성국,김영환,박승춘,Bae, Jae-sung,Jang, Kwang-ho,Rhee, Man-hee,Jeong, Kyu-shik,Jo, Woo-sik,Choi, Sung-guk,Kim, Young-hoan,Park, Seung-chun 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.3

진흙버섯 품종별 P. linteus, P. baumii 그리고 P. gilvus에 대한 형태학적 차이, 일반성분 및 무기성분을 조사하였다. 전자현미경 사진에서 P. gilvus와 P. linteus는 비슷한 형태를 보여주고 있다. 원소 함량의 구성비에서는 세 종류의 버섯이 모두 유사함을 보여주었다. 일반성분을 분석 비교시 수분의 함량은 P. baumii가 가장 높았으며 P. gilvus의 수분 함량은 10% 이내로 낮은 경향을 보여주었다. 식이섬유는 약 30% 이상을 함유하고 있으나 vitamin C 및 단백질의 함량은 높지 않았다. 원소분석에서 Ca의 경우 세 종류의 진흙버섯에서 P. baumi가 가장 높은 Ca 함량을 보여주었으나 통계적유의성은 없었다. 그러나 원소조성 및 일반성분 비교에서는 P. linteus, P. baumii 그리고 P. gilvus의 큰 차이점이 나타나지 않았다. The purpose of this study is to compare the morphology, general composition, elemental composition and mineral contents of Phellinus linteus, Phellinus baumii and Phellinus gilvus. In the scanning electron microscopy, P. gilvus and P. linteus had a similar shape. In the elemental composition, all of the three Phellinus spp. showed similar percent for the analyzed components. In the general composition, the water content of P. linteus. P. baumii and P. gilvus were 13%, 15% and 10%, respectively. The dietary fiber of three Phellius spp. showed more than 30%. The range of vitamin C and protein content was 1.5-2.1 g% and 3.63-3.73 g%. respectively. In mineral analysis, P. baumii of the three Phellinus spp. has the highest calcium concentration (1,135 ppm). From the above results, P. linteus. P. baumii and P. gilvus did not show any differences in the general composition and elemental composition.

흰쥐 혀, 혀밑샘, 귀밑샘 및 턱밑샘 비만세포에 미치는 N-Nitrosodimethylamine 독성에 대한 상황버섯의 완화효과

곽인경,조운복 釜山大學校 師範大學 科學敎育硏究所 2009 科學敎育硏究報 Vol.36 No.-

This study was made to determine alleviating effects of Pbellinus Iiateus on the NDMA toxicity to mast cell in the tongue, sublingual gland, parotid gland and submandibular gland of rat. Phellinus Iiateus (Hymenocsetscese) is a member of Basidiomycotinae, which has been used for the treatment of gastric cancer, non-insulin-dependent diabetes mellitus, diarrhea, and menstrual irregularity. Sprague-Dawley male rats weighing about 200-250gr were randomly divided 5 groups including the control, NDMA group plus sub-groups with Pbellinus linteus extract administration. DN administration group which was administrated by only 0.004% NDMAin the drinking water for one week, PN administration group which was fed by both 0.004% NDMAand Pbellinus Iinteus for one week, P+PN administration group which was applied with both 0.004% NDMAand Pbellinus Iiateus for one week after Phellinus linteus in the drinking water was dosed for one week and P+PN+P administration group which was applied with Phellinus Iinteus in the drinking water for one week after both 0.004% NDMA and Pbelliaus liateuswas fed for one week after Pbellinus Iinteus in the drinking water was dosed for one week. In order to study on the morphological changes of mast cells in the rat, toluidine blue staining was used, and to find the histological changes, H-E and PAS staining were used. The cells were counted by randomly choosing 60 visual fields under x400 magnification according to intensity of stain. The morphological changes in DN administration group, the mast cells in the tongue, sublingual gland, parotid gland and submandibular gland of rat were most irregular shaped, the granules of the cells showed more vacuolation. Degenerative mast cells were abnormally large-sized. Also mean number of total mast cell, and A type, B type dramatically increased compared to the control group. The groups of feeding Phellinus linteus showed recovering tendency. The recovery degree of P+PN administration group was greater than that of PN administration group. And P+PN+P administration group recovered almost up to the state of the control group. Mean number of total mast cell in the rat tongue was 4.08±3.38. In the DN administration group, the mean number of total mast cell was 12.28±6.25, in the PN administration group was 9.25±6.08, in the P+PN administration group was 4.96±4.59,and in the P+PN+P administration group was 3.81±3.68. It turned out that the pattern of total mast cell in the rat sublingual gland, parotid gland and submandibular gland was the same with above. The total mast cell and A type, B type numbers were significantly decreased by the treatment of Phellinus linteus, degranulation of mast\ cells were markedly inhibited by Phellinus linteus. P+PN+P administration group recovered almost up to same size and shape as the control group. Consequently, the above results suggest that NDMA exhibit the toxicity which affects the mast cell in the tongue, sublingual gland, parotid gland and submandibular gland of rat and also suggest that Phellinus linteus alleviates this toxicity. The different results of recovering tendency of NDMA toxicity in PN administration group, P+PN administration group and P+PN+P administration group indicate that the alleviating effects of Phellinus linteus on NDMA toxicity to the mast cell in the tongue, sublingual gland, parotid gland and submandibular gland depend on the dosing time and the amount of Phellinus linteus. Furthermore, Phellinus linteus is presumed to have effects to recover from NDMA toxicity, to suppress exhibition of NDMA toxicity and to prevent NDMA toxicity. From these results, it was suggested that Phellinus linteus can help to recover the damaged function and further may help to prevent senescence diseases.

수 종 상황버섯의 조다당 분획물 조성

박미정(Mijung Park),안미정(Mi-Jeong Ahn),김진웅(Jinwoong Kim),제금련(Keum Ryon Ze),이흠숙(Heum Sook Lee) 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.1

본 연구는 국내에서 재배되거나 수입되어 유통되는 4종 Phellinus 속 버섯(구멍쟁이버섯과)의 조다당 분획물의 조성을 비교함으로써 각 종간의 차이에 의한 성분조성의 차이를 검토하기 위하여 수행되었다. 상황버섯의 활성 분획 중의한 유효성분인 조다당 분획물을 에탄올 냉침법으로 제조한 결과, 조다당 분획물의 취득률은 P. linteus 15.0%, P. baumii 5.1%, P. pini 6.3%, P. igniarius 5.7%로 Phellinus 종에 따라 차이가 있었다. 또한, 그 다당체를 조성하고 있는 단당의 함유량도 버섯의 종에 따라 큰 차이를 보였다. 즉, P. linteus의 경우, 건조중량 1 g 당 함유된 당의 총량은 148.713 ㎎/g이었으나, P. baumii는 29.022 ㎎/g, P. pini는 29.222 ㎎/g으로 P. lintues의 약 1/5 정도의 당 함유량을 나타냈으며, P. igniarius는 P. lintues의 1/100 정도인 1.546 mg/g의 당을 함유하고 있었다. 모든 Phellinus 속 버섯에서 glucose의 함량이 월등하게 많았으며, 함유되어 있는 각 단당들의 절대량은 종에 따라 차이가 있었다. P. linteus는 mannose와 ribose가 P. baumii와 P. pini보다 적은 양이 함유되어 있는 것을 제외하고는 그 외의 모든 단당을 월등하게 많이 함유하고 있었다. P. baumii와 P. pini는 arabinose를 제외하고는 거의 모든 단당의 함량이 유사하였다. P. igniarius는 거의 모든 당의 절대량이 다른 Phellinus 속 버섯에 비하여 매우 적었다. Various Phellinus species (Hymenochaetaceae) including P. linteus, P. baumii, P. pini, and P. igniarius of domestic and foreign products, were examined by gas chromatography to validate their composition of crude polysaccharides. The crude polysaccharide fraction, which is known to be one of the physiologically active fractions, were prepared by a cold ethanol precipitation method and the yield of the crude polysaccharide fractions of P. linteus, P. baumii, P. pini, and P. igniarius were 15.0%, 5.1%, 6.3% and 5.7%, respectively. The total monosaccharide amount of the crude polysaccharide fraction of P. linteus was 148.713 ㎎/g (dry weight of mushroom) while those of P. baumii, P. pini and P. igniarius were 29.022 ㎎/g, 29.222 ㎎/g and 1.546 ㎎/g, respectively. Although the major monosaccharide of all Phellinus mushrooms was glucose, there were remarkable differences in the absolute quantity of each monosaccharide. P. linteus included plenty of monosaccharides except mannose and ribose in comparison with P. baumii and P. pini. The absolute quantities of monosaccharides of P. baumii and P. pini were nearly similar except arabinose. The total monosaccharides of P. igniarius were minimal, but the portions of galactose and mannose in P. igniarius were greater than in the other species.

복강암세포(Sarcoma-180)를 이식한 마우스에서 상황버섯(Phellinus linteus) 추출물이 백혈구 화학주성(Chemotaxis)능에 미치는 영향

조홍식,윤정중,이연태 대한암예방학회 1999 Journal of cancer prevention Vol.4 No.4

기주식물에 따른 목질진흙버섯의 일반성분(무기물) 함량변화 I

이수원 외 한국버섯학회 2005 한국버섯학회지 Vol.3 No.3

We investigated mineral difference from mushroom Phellinus linteus and Phellinus baumii on mulberry, oak and elm. ICP test, The major mineral components were K, Ca, and Fe, while Mg, Na, P, Cr, Mn, Cu and Zn were determined as micro mineral components. In case of P.linetus and P.baumii the component of K was shown as 699.0mg/100g and 630mg/100g, in oak, 340.7mg/100g and 314.6mg/100g in elm, and 311.6mg/100g and 311.6mg/100g in mulberry respectively. In Ca, mulberry fruiting body were shown about 2 times highest and expecially in case of Na was oak fruiting body were shown as 10 times highest as against the others sample groups. In micro mineral components, Zn component was shown very diffeence about sample groups. We concluded that the highest mineral component of K was shown in mushroom cultivated on oak of both P.linetus and P.baumii. As a results, mineral component has showed very difference on cultivated to timber. According to the results of mineral component we concluded that Phellinus linteus and Phellinus baumii has mineral component different depending on timber. 두 종류의 목질진흙버섯인 P. linteus 와 P. baumii 를 뽕나무, 참나무, 느릅나무등 3가지의 기주식물에서 자실체를 발생시켜 무기물의 함량을 ICP 법으로 측정하였다. 각각의 균주 및 기주식물에 따라 무기물의 커다란 함량 차이를 확인하였다. 다량 함유 무기물중 K은 두 균주 모두 가장 많은 무기물 함량을 보였으며 참나무에서 재배한 자실체의 K의 함량이 두배 정도 높았고 Ca은 뽕나무의 함량이 특히 높았다. Fe은 특히 느릅나무에서 재배한 자실체의 Fe 함량이 월등히 적게 함유된 것으로 확인되었다. 미량 함유 무기물은 Na에서 가장 주목할만한 결과를 확인하여 두 균주 모두 참나무에서 재배된 자실체의 Na 함량이 10배나 크게 함유되었다. 또한 P. linteus의 무기물 함량 특징은 극미량 원소중 Zn가 원목에 따른 함량으로 확인할수 있으며 Mn의 함량 또한 참나무 재배 자실체에서 3배 정도 높게 함유되었다. 그리고 무기물만의 함량으로 P. linteus 와 P. baumii 를 구별하는데 Zn의 함량을 측정할 수는 있으나 Zn 만으로는 판정하는데 어려움이 있을수 있으나 형태적으로 P. linteus 와 P. baumii 의 자실체의 모양이 차이가 뚜렷한 차이를 보여 무기물의 함량을 측정하며 P. linteus와 P. baumii 가 어떤 원목에서 재배되었는가를 판정할수 있는 자료로 연구의 가치가 있는 것으로 판단한다.

상황버섯균 접종 황기의 배양 중 이화학적 성분변화

장연정,김은주,김소영,이윤혜,박신영 한국식품저장유통학회 2016 한국식품저장유통학회지 Vol.23 No.5

This study analyzed the changes in physicochemical components of Astragalus membranaceus (AM) fermented with Phellinus linteus. Moisture content, pH, total acidity, total reducing sugar content, extraction yield, free sugar content, free amino acid and isoflavonoid (calycosin, formononetin) were investigated. The moisture content was increased during fermentation with Phellinus linteus. The pH level increased while the total acidity significantly decreased during fermentation. The reducing sugar content were in the range of 0.32~0.61%. The extraction yield using water was higher than that using 80% ethanol. The major free sugars were identified as glucose, fructose, sucrose and the content of free sugars decreased through fermentation. However, the glucose and sucrose contents of the water extracts were increased. In addition, the free amino acid increased significantly during fermentation. Finally, calycosin and formononetins contents in water extracts of after 30 days of AM fermentaion with Phellinus linteus were (3.91 mg/100 g) and (1.38 mg/100 g), respectively. These results suggest that fermentation with Phellinus linteus could be used to increase the bioactivity of AM. The mycelium-fermented AM could be a valuable source of functional material and edible resource for industry.

항보체 활성을 갖는 Phellinus linteus 균사체 생산의 최적화

서호찬 한국응용생명화학회 2010 Journal of Applied Biological Chemistry (J. Appl. Vol.53 No.3

각종 버섯에서 항보체 활성균주를 선별하기 위해 보관균주 50여종과 임업협동조합에서 분양 받은 20여종의 균주를 대상으로 항보체 활성을 검색한 결과, 항보체 활성이 65.3%를 나타낸 상황버섯(Phellinus linteus)을 선별하였다. 본 균주가 생산하는 항보체 활성의 본체를 파악하기 위해 pronase 처리와 periodate 산화를 행한 결과 pronase로 처리한 시료는 무처리군과 비교하여 차이가 없었던 반면 periodate로 산화시킨 시료는 항보체 활성이 크게 감소함에 따라 P. linteus가 생산하는 항보체 활성의 본체는 다당에 기인되는 것으로 추정되었다. 항보체 생산을 위한 최적 배양조건은 soluble starch 3.0%, peptone 0.3%, yeast extract 0.4%, MgSO4·7H2O 0.1%, K2HPO4 0.2%, 초기 pH 7.0, 배양온도 30℃ 및 교반속도 150 rpm이었다. 상기의 최적 배양조건에서 jar fermentor로 18일 배양하였을 때 88%의 항보체 활성과 15mg/mL의 균사체 생육을 나타내었다. To produce the functional food materials from edible mushrooms, hot-water extracts from 70 kinds of mushroom mycelia were examined for anti-complementary activity and Phellinus linteus showed the highest activity through the complement fixation test. The maximum production of Phellinus linteus mycelia with anti-complementary activity was observed in culture medium containing soluble starch 3.0%, peptone 0.3%, yeast extract 0.4%, MgSO4·7H2O 0.1%, K2HPO4 0.2% and in the culture conditions controlled at initial pH 7.0, 30℃ and 150 rpm by the rotary shaker. In addition, the maximum production of mycelial dry weight was 15 mg/mL after 18 days under the optimal conditions, and anti-complementary activity was reached to 88% in 5 L-jar fermenter.

Artificial cultivation of Medicinal Mushroom, Phellinus linteus using Mulberry logs

Hong, In-Pyo,Sung, Gyoo-Byung,Chung, In-Mo,Lee, Won Chu 한국잠사학회 2002 한국잠사곤충학회지 Vol.44 No.2

The optimal conditions for mycelial growth of P. linteus ASI 26011 were 25-30°C and pH 6.0, respectively. The mycelial growth of p. linteus was excellent on MCM medium. In case of carbon sources, the mycelial growth of P. linteus was best on the culture media that were contained with sucrose,k mannose and glucose. Potassium nitrate and sodium nitrate were good for the mycelial growth of p. lingeus as a nitrogen source. For comparison of the mycelial colonization of P. linteus on logs, several techniques of inoculation were tested; the sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation. The mycelial colonization of p. linteus on logs was good in the treatment of sterilized short log inoculation, but poor in the traditional methods such as drilling inoculation and log- end sandwich. The initial mycelial growth and the full cycelial colonzation of p. linteus were the best on 20cm logs under the condition of 42% of moisture content in log. Also the initial mycelial growth of P. linteus was accelerated over 12 hours of sterilization. Burying method of logs after 5-6 months of incubation was the best for formation of basidiocarp of p. linteus. The formation of fruiting body of P. linteus was quite good in the cultivation house at the 31-35°C and over 96% of relative humidity.

A Sphingolipid and Tyrosinase Inhibitors from the Fruiting Body of Phellinus linteus

Hye Sook Kang,Jin Ho Choi,Won Ki Cho,Jong Cheol Park,Jae Sue Choi 대한약학회 2004 Archives of Pharmacal Research Vol.27 No.7

This paper for the first time reports the isolation of 5 compounds from Phellinus linteus. A sphingolipid (1) and two tyrosinase inhibitory compounds (2, 3) along with two carboxylic acids (4, 5), were isolated from the fruiting body of Phellinus linteus (Berk & Curt) Aoshima. The structure of compound 1 was identified as 1-O-b-D-glucopyranosyl-(2S, 3R, 4E, 8E)-2-[(2R)-2- hydroxyhexadecanoylamino]-9-methyl-4,8-octadecadiene-1,3-diol, known as cerebroside B, based on spectroscopic methods such as 1D and 2D NMR as well as by acid hydrolysis. Compounds 2~5 were identified as protocatechualdehyde (2), 5-hydroxymethyl-2-furaldehyde (HMF) (3), succinic acid (4), and fumaric acid (5) based on the spectroscopic evidence. Compounds 2 and 3 inhibited the oxidation of L-tyrosine catalyzed by mushroom tyrosinase with an IC50 of 0.40 and 90.8 mg/mL, respectively. The inhibitory kinetics, which were analyzed by the Lineweaver-Burk plots, were found to be competitive and noncompetitive inhibitors with a Ki of 1.1 mM and 1.4 mM, respectively.

상황보리 녹차의 휘발성 향기성분

최성희(Sung Hee Choi),정경태(Kyung Tae Chung) 한국차학회 2009 한국차학회지 Vol.15 No.1

To make tea having good flavor and functional property, roasted Phellinus linteus-barley(50%) was added to green tea(50%). Volatile flavor components of the tea were analyzed and identified. The extraction of volatile flavor compounds of the tea was accomplished by a simultaneous distillation and extraction method using a Likens and Nickerson's extraction apparatus. The concentrated extract was analyzed by GC-mass spectrometry. The main volatile flavor components of Phellinus linteus baley-green tea were compounds that originated from roasted Phellinus linteus-barley and green tea. The former main volatile flavor components were 4-methyl-2-methyl phenol, furanones and furfural etc. The latter main volatile flavor components were linalool, phenylethyl alcohol, β-ionone and geraniol etc.