유산소 운동이 Streptozotocin 유도 당뇨쥐 생식세포의 생화학적 특성에 미치는 영향

임은미(EunMiLim),김유미(YouMiKim),윤진환(JinHwanYoon),김광찬(KwangChanKim) 한국체육학회 2013 한국체육학회지 Vol.52 No.3

본 연구는 유산소 운동이 Streptozotocin(STZ) 유도 당뇨 쥐 고환조직의 생화학적 특성에 어떤 영향을 미치는지 규명하는데 그 목적이 있다. 이를 위해 10주령의 Sprague-Dawley 계열 수컷 흰 쥐를 통제그룹(CON; n=15), 당뇨그룹(DM; n=15), 당뇨운동그룹(DM-EX; n=15)으로 무선배정 하였으며, 12주간(5일/주, 60분/일) 무부하 수영운동을 실시하였다. 연구결과 당뇨그룹은 glucose transporter-3 (GLUT-3), lactate dehydrogenase C-4 (LDHC-4), monocarboxylate transporter-2 (MCT-2)의 발현이 통제 집단에 비해 유의하게 감소하였다(p<.05). 반면 당뇨운동그룹은 GLUT-3, LDHC-4, MCT-2의 발현이 당뇨그룹에 비해 유의하게 증가 하였다(p<.05). 이러한 결과를 종합해 볼 때 수영운동은 당뇨환자의 고환조직 생화학적 기능을 향상시켜 주는데 효과가 있는 것으로 나타났다. The purpose of this study was to investigate the effects of aerobic exercise upon characteristic of biochemistry on of Testicular tissue in the streptozotocin (STZ)-induced diabetic rats. 10 week-old male Sprague-Dawley rats were divided into control group (CON; n=15), diabetes group (DM; n=15) and diabetes-exercise group (DM-EX; n=15). The rats in DM-EX group was made to swim(5 days/week, 60 min/day) for 12 weeks without load. In DM group, glucose transporter-3 (GLUT-3), lactate dehydrogenase C-4 (LDHC-4) and monocarboxylate transporter-2 (MCT-2) protein reduced in the testicular tissue compared to the CONl group (p<.05). In contrast, in DM-EX group, GLUT-3, LDHC-4 and MCT-2 protein enhanced in the testicular tissue compared to the DM group (p<.05). These results showed that swimming exercise improves functions of biochemistry of testicular tissue in the diabetes.

Effects of Red Ginseng on Exercise Capacity and Peripheral Fatigue in Mice

김대현(Daehyun Kim),이병관(Byounggwan Lee),김희진(Heejin Kim),김미경(Mikyung Kim) 물리치료재활과학회 2021 Physical therapy rehabilitation science Vol.10 No.2

Objective: Fatigue can decrease both quality of life and work efficiency. Ginseng is one of the most popular herbal treatments forimproving personal health, with applications in treating fatigue. However, the exact mechanisms of anti-fatigue effects are stillunclear. Thus, we investigated the effect of red ginseng powder (RGP) on exercise capacity and peripheral fatigue using bothbehavioral and molecular experiments in mice. Design: Four-groups behavioral and molecular experiment. Methods: Male 6-weeks-old ICR mice were treated with distilled water, 100, and 200 mg/kg RGP for 5 days via oraladministration. The exercise capacity of each animal group was measured by locomotor activity, rota-rod, hanging wire, and coldswimming tests. Additionally, after performing the treadmill to induce fatigue, lactate expression and molecular experiments were investigated using mice gastrocnemius. Results: Mice treated with RGP exhibited increased exercise capacity in the behavioral tests. Additionally, RGP induced a dose-dependent decrease in lactate levels after high-intensity exercise, and Monocarboxylate transporter (MCT) 4 expressionincreased in groups treated with RGP. However, there was no significant change in MCT1. Conclusions: These results suggest that RGP exerts several anti-fatigue properties by lower lactate and improved exercisecapacity. Increased MCT4 expression may also affect lactate transport. Thus, this study suggests that the anti-fatigue properties ofRGP might be associated with MCT4 activity.

유산소운동이 당뇨쥐 해마의 신경세포 LDH 동위효소, MCT2 및 GLUT3 단백질 발현에 미치는 영향

이상학,김종오,윤은정 한국생활환경학회 2020 한국생활환경학회지 Vol.27 No.5

In the present study, the effects of aerobic exercise on expression of Lactate dehydrogenase (LDH) isozymes, Monocarboxylate transporter2 (MCT2) and Glucose transporter3 (GLUT3) in hippocampal neuron of Streptozotocin (STZ)-induced diabetic rats. The experimental animals were divided into three groups (n=10 in each group): the normal control group (CON), the diabetes mellitus group (DM), the diabetes and exercise group(DM-Ex). Animals in the exercise group were submitted to daily exercise in the water pool during 4weeks. Native polyacrylamide gel electrophoresis analysis for the purification of LDH isozyme was performed. Western blotting and immunohistochemistry analysis for the expression of MCT2 and GLUT3 was performed. Here in this study, LDH A4 and LDH A3B in DM were predominantly expressed compared to DM-Ex (p<.05), and LDH AB3 and LDH B4 in DM-Ex were predominantly expressed compared to DM (p<.05). MCT2 activity of DM and DM-Ex were significantly lower than that of CON (p<.05). MCT2 activity of DM-Ex was significantly higher than that of DM (p<.05). GLUT3 activity of DM and DM-Ex were significantly lower than that of CON (p<.05). GLUT3 activity of DM-Ex was significantly higher than that of DM (p<.05). The results of the present study indicate that aerobic exercise affects the production of ATP using lactic acid and the activity of neurons by adapting to the energy metabolic demands of diabetes in the hippocampal neurons.

High Monocarboxylate Transporter 4 Protein Expression in Stromal Cells Predicts Adverse Survival in Gastric Cancer

Yan, Ping,Li, Yu-Hong,Tang, Zhi-Jiao,Shu, Xiang,Liu, Xia Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Background: Increasing evidence suggests that stromal monocarboxylate transporter 4 (MCT4) and carbonic anhydrase IX (CA IX) may play key roles in tumor development. However, their clinical value remains largely unexplored in gastric cancer (GC). The present study aimed to determine clinicopathological significance and prognostic values of stromal MCT4 and CA IX in GC. Materials and Methods: Specimens from 143 GC patients were immunohistochemically stained using polyclonal anti-MCT4 and anti-CA IX antibodies. Expression was correlated with patient clinicopathologic characteristics and survival data. Results: High stromal MCT4 expression was detected in 72 of 143 (50.3%) GCs and high CA IX in 74 (51.7%). Both high stromal MCT4 and CA IX were correlated with advanced TNM stage (p=0.000; p=0.000). High CA IX expression was positively related to depth of invasion (p=0.022) and positive lymph nodes (p=0.002) as well. Survival analysis indicated high expression of stromal MCT4 to be an independent factor in predicting poor overall survival (OS) (HR and 95%CI=1.962, 1.032-3.729, p=0.040) and disease free survival (DFS) (HR and 95%CI=2.081, 1.158-3.741, p=0.014) of GC patients. However, high CA IX expression exhibited no significant predictive value. Conclusions: These findings suggest that high expression of stromal MCT4 and CA IX proteins is significantly correlated with GC progression. High stromal MCT4 heralds worse outcome of GC patient, suggesting a novel candidate prognostic marker and therapeutic target.

The genetic variation in Monocarboxylic acid transporter 2 ( <i>MCT2</i> ) has functional and clinical relevance with male infertility

Lee, Jinu,Lee, Dong Ryul,Lee, Suman Medknow PublicationsMedia Pvt Ltd 2014 ASIAN JOURNAL OF ANDROLOGY Vol.16 No.5

<P>Monocarboxylic acid transporter 2 (MCT2) transports pyruvate and lactate outside and inside of sperms, mainly as energy sources and plays roles in the regulation of spermatogenesis. We investigated the association among genetic variations in the <I>MCT2</I> gene, male infertility and <I>MCT2</I> expression levels in sperm. The functional and genetic significance of the intron 2 (+28201A > G, rs10506398) and 3’ untranslated region (UTR) single nucleotide polymorphism (SNP) (+2626G > A, rs10506399) of MCT2 variants were investigated. Two MCT2 polymorphisms were associated with male infertility (<I>n</I> = 471, <I>P</I> < 0.05). In particular, the MCT2-3’ UTR SNP (+2626 G > A) had a strong association with the oligoasthenoteratozoospermia (OAT) group. The +2626GG type had an almost 2.4-fold higher sperm count than that of the +2626AA type (+2626GG; 66 × 10<SUP>6</SUP><I>vs</I> +2626AA; 27 × 10<SUP>6</SUP>, <I>P</I> < 0.0001). The MCT2-3’ UTR SNP may be important for expression, as it is located at the MCT2 3’ UTR. The average MCT2 protein amount in sperm of the +2626GG type was about two times higher than that of the +2626AA type. The results suggest that genetic variation in MCT2 has functional and clinical relevance with male infertility.</P>

Monocarboxylate transporter 9 (MCT9) is down-regulated in renal cell carcinoma

Ara Yoo,Hyeonhee Lee,Jinyoung Jung,Sang Seok Koh,이수진 한국유전학회 2021 Genes & Genomics Vol.43 No.4

Background The renal cell carcinoma (RCC) incidences are continuously increasing, however, their proper characterization remains difcult. Mammalian kidneys require large amounts of energy, and monocarboxylate transporter (MCT) gene family is responsible for the transport of monocarboxylic compounds across plasma membranes. Objective A total of 14 MCT members have been identifed in humans, which show highly distinct substrate afnities and tissue distributions. To understand the yet-uncharacterized renal cancer-specifc role of MCTs, we identifed MCT members that are diferentially regulated during the renal tumor progression. Methods We examined the expression level of MCT members in renal cell tumors and their relationship with survival rate of patients using a public database. Quantitative RT-PCR and northern blotting were performed to validate the expression of MCTs. Anti-MCT9 antiserum was raised in rabbit and used to examine MCT9 expression in normal and tumor tissue arrays. Efect of MCT9 overexpression on cell proliferation was measured using renal cancer cell lines. Results MCT9 was found to be abundantly and exclusively expressed in human kidney cells, and was highly downregulated in renal cancers. Kaplan–Meier plotter analysis revealed an increased survival rate of MCT9 high-expressing RCC patients. MCT9 proteins were detected in normal kidney tissue sections and their overexpression clearly attenuated renal cell proliferation. Conclusions MCT9 was identifed as a novel highly downregulated gene in renal cell cancer, and its overexpression clearly attenuated RCC cell proliferation. Thus, functional analysis of MCT9 may help in deciphering a yet-undiscovered kidneyspecifc energy metabolism during renal tumor progression.

The Expression of Glut-1, CAIX, and MCT4 in Mucinous Carcinoma

김세화,정우희,구자승 한국유방암학회 2013 Journal of breast cancer Vol.16 No.2

Purpose: The aim of this study was to assess the expression of metabolism-related proteins including glucose transporter 1 (Glut-1), carbonic anhydrase IX (CAIX) and monocarboxylate transporter 4 (MCT4) in breast mucinous carcinoma and to evaluate the implications of the results. Methods: Immunohistochemical staining for Glut-1, CAIX, and MCT4 was performed on tissue sections from 59 cases of mucinous carcinoma to evaluate the association between the expression of metabolism-related proteins and clinicopathologic factors. Mucinous carcinoma was subclassified into type A and type B according to histopathological characteristics. Results: Of the 59 patients, 35 patients (59.3%) were type A mucinous carcinoma and 24 patients (40.7%) were type B mucinous carcinoma. Stromal expression of MCT4 was significantly associated with a high histologic grade (p=0.022) and type B mucinous carcinoma (p=0.016). There were significant positive correlations between the expression of Glut-1, CAIX and tumoral expression of MCT4 (p<0.05). Conclusion: We assessed the expression of metabolism-related proteins including Glut-1, CAIX, and MCT4 in breast mucinous carcinoma and found that the stromal expression of MCT4 was higher in type B mucinous carcinoma than in type A, which reflected a difference in the tumor microenvironment.

흰쥐의 부정소에서 Monocarboxylate Transporters(MCTs)와 조절 단백질, Basigin과 Embigin의 생후 발달 과정 동안 발현 양상

이기호 ( K. H. Lee ) 한국동물자원과학회 2008 한국축산학회지 Vol.50 No.1

번식생리 및 내분비 : 생후 발달과정동안 남성 생식기의 Efferent Ductules에서 Monocarboxylate Transporters (MCTs)와 Basigin의 발현 양상과 에스트로젠 수용체 α에 의한 MCT1 발현 조절 연구

이기호 ( K. H. Lee ),최인호 ( I. H. Choi ),정정수 ( C. S. Chung ),장종수 ( J. S. Chang ) 한국동물자원과학회 ( 구 한국축산학회 ) 2007 한국축산학회지 Vol.49 No.3

Allan-Herndon-Dudley Syndrome 환아에서 발견된 novel c.826G>A Mutation

신은경(Eun-Kyung Shin),박병한(Byung Han Park),문진화(JinHwa Moon),김자혜(Ja Hye Kim),유한욱(Han-Wook Yoo),김구환(Gu-Hwan Kim) 대한소아신경학회 2017 대한소아신경학회지 Vol.25 No.3

Allan-Herndon-Dudley syndrome (AHDS) is an X-linked intellectual disability caused by monocarboxylate transporter 8 (MCT8) deficiency. AHDS manifests in global developmental delay, axial hypotonia, quadriplegia, movement disorders in male patients, and most of them show the delayed or hypomyelination on brain magnetic resonance images. Typically, Triiodothyronine (T3) levels are markedly elevated, thyroid stimulating hormone (TSH) levels are normal or elevated, and free thyroxine (T4) levels are normal or decreased. In AHDS patients, early neurological manifestations are easily mistaken as cerebral palsy with unknown origin. Here, we present a novel c.826G>A mutation in a boy with severe axial hypotonia, limb dystonia and developmental delay. Thyroid function test including TSH, T3, and free T4 levels was the important clue for the diagnosis of AHDS of the patient.