닭유래 Escherichia coli의 병원성에 관한 연구 : 시험관내 Congo-red 결합능과 병원성간의 상관관계

우용구,김기석,김봉환,Woo, Yong Ku,Kim, Ki Seuk,Kim, Bong Hwan 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.1

The correlation between in vitro Congo-red binding properties of E coli and in vivo invasiveness of the organisms in SPF chickens and mice was investigated. Congo-red positive E coli colonies were dark-red color with a typical colonial morphology of rough appearance when grown on Congo-red medium, while Congo-red negative colonies showed pale-pink color and smooth surfaced colonial morphology. Pathogenicity of 10 Congo-red positive E coli for mice was observed in 92.5% but that of 5 Congo-red negative E coli was 45%. Invasiveness of 10 Congo-red positive E coli for chickens was observed in 96% of the SPF chickens tested but that of 5 Congo-red negative E coli was 16% only. These results of pathogenicity studies with E coli isolates indicate a significant correlation between Congo-red binding ability and virulence in avian Escherichia coli.

Hydrogen Peroxide, Its Measurement and Effect During Enzymatic Decoloring of Congo Red

( Woo Sung Whan ),( Jeung Suk Cho ),( Byung Ki Hur ),( Dong Hoon Shin ),( Keun Gap Ryu ),( Eun Ki Kim ) 한국미생물생명공학회 2003 Journal of microbiology and biotechnology Vol.13 No.5

Development of Congo red broth method for the detection of biofilm-forming or slime-producing <i>Staphylococcus</i> sp.

Lee, Jung-Su,Bae, Young-Min,Han, Areum,Lee, Sun-Young Elsevier 2016 FOOD SCIENCE AND TECHNOLOGY -ZURICH- Vol.73 No.-

<P><B>Abstract</B></P> <P>Although the Congo-red agar method has been widely used to detect slime-producing or biofilm forming <I>Staphylococcus</I> sp. in numerous previous studies, their results were contradictory and did not show high correlation with other biofilm characteristics of bacteria such as <I>ica</I> gene profiles, biofilm formation, and so on. Therefore, this study was conducted to evaluate various Congo-red agar methods and to develop the accurate detection method for slime-producing <I>Staphylococcus</I> sp. using Congo-red dye. Total 47 <I>Staphylococcus</I> sp. (36 <I>Staphylococcus aureus</I> and 11 other strains) were tested for the <I>ica</I> gene profiles and phenotypes on various Congo-red agars or broths based on TSB, BHI, or BAB with sucrose or glucose. The <I>ica</I>A/<I>ica</I>D genes were found in 32 <I>S. aureus</I> and 5 other strains and these results was perfectly correlated with results on Congo-red broth based on TSB supplemented with sucrose. Relatively high positive-predictive values (84–93%) were observed when Congo-red agars were tested while negative-predictive values were low (26–44%). From these results, phenotypes on Congo-red agar or broth methods could be different depending on types or chemical composition of tested media and Congo-red broth method based on TSB supplemented with sucrose designed in this study were very accurate to detect slime-producing <I>Staphylococcus</I> sp.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new TSB based Congo-red (CR) broth method was developed in this study. </LI> <LI> CR agar or broth methods were different depending on types or composition of media. </LI> <LI> CR TSB broth method was correlated with presence of <I>icaA/D</I> gene of <I>Staphylococcus</I> sp. </LI> </UL> </P>

활성탄에 의한 Congo Red의 흡착에 대한 등온선, 동력학 및 열역학적 특성

이종집 ( Jong Jib Lee ) 한국화학공학회 2015 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.53 No.1

본 연구는 활성탄에 의한 congo red의 흡착 거동에 대해 회분식 실험을 통해 알아보았다. 흡착변수로 흡착제의 양, pH, 초기농도와 접촉시간과 온도를 사용하였다. 흡착평형자료는 Langmuir, Freundlich, Temkin 및 Dubin-Radushkevich식에 적용하여 보았다. 평가된 Freundlich 분리계수(1/n)로부터 활성탄에 의한 congo red의 흡착공정이 적절한 처리방법이 될 수 있음을 알았고, Temkin 상수(B)와 Dubinin-Radushkevich 상수(E)로부터 물리흡착공정임을 알았다. 동력학적 실험을 통해 흡착공정이 유사이차반응속도식에 잘 일치함을 알았다. 입상활성탄에 대한 congo red의 흡착공정은 발열반응(ΔH=42.036 kJ/mol)이었고, Gibbs 자유에너지값(ΔG=-2.414~-4.596 kJ/mol)은 온도가 증가할수록 감소하였다. Batch experiment studies were carried out for adsorption of congo red using granular activated carbon with various parameters such as activated carbon dose, pH, initial dye concentration, temperature and contact time. Equilibrium experimental data are fitted to the Langmuir, Freundlich, Temkin and Dubin-Radushkevich isotherm equations. From Freundlich`s separation factor (1/n) estimated, adsorption could be employed as effective treatment method for adsorption of congo red from aqueous solution. Base on Temkin constant (B) and Dubinin-Radushkevich constant (E), this adsorption process is physical adsorption. Adsorption kinetics has been tested using pseudo-first order and pseudo second order models. The results followed pseudo second order model with good correlation. Adsorption process of congo red on granular activated carbon was endothermic (ΔH=42.036 kJ/mol) and was accompanied by decrease in Gibbs free energy (ΔG=-2.414 to -4.596 kJ/mol) with increasing adsorption temperature.

INVESTIGATING THE ANTIBIOSIS OF SMART PHONE INHABITING BACTERIA BY USING QUORUM-QUENCHING EFFECT OF GARLIC

Ye Ji KIM 국제과학영재학회 2014 APEC Youth Scientist Journal Vol.6 No.2

As information technology is developing, the Smart phone became one of the ways to spread diseases. However, most people still do not care about contamination from Smart phones. At least, some are using antibacterial coating to cleanse the liquid crystal display, but these antibacterial coatings have latent dangers especially the nano materials. If silver accumulates in the body, it doesn’t exit easily even if people use hemodialysis and heavy metal removal. Therefore the goal of this research is to make safe antibacterial smart coatings. This research will focus on Quorum Quenching effect of onion and garlic. Quorum Quenching inhibits the signaling responses include adaptation to availability of nutrients, defense against other microorganisms which may compete for the same nutrients and the avoidance of toxic compounds potentially dangerous for the bacteria. It is very important for pathogenic bacteria during infection of a host to co-ordinate their virulence in order to escape the immune response of the host in order to be able to establish a successful infection. In this research, we used Escherichia coli, Staphylococcus aureus, Salmonella enteric, which are three representative bacteria that inhabits in Smart phones. Experiments were conducted using Congo Red staining method and Crystal violet staining method.

Enterobacter sp. JE-1에 의한 Congo Red의 생분해

공은진,김종수,이건,이상준,이종근 한국환경과학회 1998 한국환경과학회지 Vol.7 No.4

The bacterial strain JE-1 degrading and utilizing Congo Red as a sole carbon source was isolated from dye-contaminated soil and identified as Enterobacter species. Enterobacter sp. JE-1 had the highest decolorization ability when it was cultured in the medium containing 0.05% NH_4NO_3, 0.05% K_2HPO_4 0.03% MgSO_4·7H_2O, 0.025% Congo Red, initial pH 7.0 at 30℃, respectively. Enterobacter sp. JE-1 had the relatively high substrate specificity. The dye decolorizing activity was exclusively extracellular. The expected metabolic intermediates of Congo Red by Enterobacter sp. JE-1 were analyzed by GC/MS. As a result, metabolic products like hexadecanoic acid, 1,2,3-triphenylcyclopropene, aliphatic hydrocarbons such as hexadecane, heptadecane, octadecane, hexacosane etc., and 1,2-benzenedicarboxylic acid, dibutyl ester were detected. Benzidine did not detected.

ZnO/rutile-$TiO_2$, ZnO, rutile-$TiO_2$, CdS를 이용한 Congo red의 광 촉매 분해반응

김창석,류해일,Kim, Chang Suk,Ryu, Hae-Ill 한국분석과학회 2001 분석과학 Vol.14 No.3

ZnO, CdS, rutile-$TiO_2$ 및 혼합 rutile-$TiO_2$/ZnO와 같은 여러 반도체를 이용하여 Congo Red를 광 촉매 분해시켰다. 연구 결과 ZnO, CdS, rutile-$TiO_2$ 중에서는 CdS의 광 촉매 효과가 제일 컸는데 이것은 CdS가 제일 작은 band gap 에너지를 가지고 있기 때문이었다. 또한 혼합 촉매에서는 ZnO의 함량이 rutile-$TiO_2$에 비하여 상대적으로 높을수록 분해 반응을 촉진하였다. 이것은 $Zn^{2+}$ 가수분해 생성물이 구조적으로 안정한 화합물인 rutile-$TiO_2$의 표면을 덮음으로서 자외선 흡수를 차단하기 때문에 라디칼 생성을 저해하기 때문이었다. Photocatalytic degradation of Congo red was performed using various semiconductors as ZnO, CdS, rutile-$TiO_2$ or mixed rutile-$TiO_2$/ZnO. The change of degradation of the dye was investigated by UV-visible spectrophotometric method. The photocatalytic action of CdS was greater than ZnO and rutile-$TiO_2$ in account of low band gap energy of CdS. The rate of photocatalytic degradation reaction increased drastically in according to increasing ratio of ZnO on mixed rutile-$TiO_2$/ZnO. These photocatalytic effect of rutile-$TiO_2$ was suppressed by more stable rutile-$TiO_2$, doping the hydrolysis product with $Zn^{2+}$ prior to calcination onto the rutile-$TiO_2$ surface.

Activating natural bentonite as a cost-effective adsorbent for removal of Congo-red in wastewater

Manjot Toor,Bo Jin,Sheng Dai,Vipasiri Vimonses 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.21 No.1

The bentonite is a widely available and abundant natural mineral, and can be a low cost adsorbent forwater and wastewater treatment. This study reported here was directed towards identifying a costeffectiveactivation protocol for enhancing the adsorption capacity of Australian bentonite for removal oftoxic contaminants in wastewater. We investigated three protocols including thermal activation (TA),acid activation (AA), and combined acid and thermal activation (ATA). The results showed that theseactivation protocols under designed conditions can enhance the surface area and porosity of the rawbentonite. The best ATA protocol considered here brought a 70% increase in the surface area compared to65% and 20% for the best AA and TA protocols, respectively. The optimal ATA protocol identified in thestudy leads to approximately 25% increase in the Congo-red adsorption capacity of the activatedbentonite. This activation method could be a cost-effective approach to enhance the adsorption capacity,applicability and selectivity of natural clay materials,making them as promising and low cost adsorbentsfor wastewater treatment.

Efficient and practical adsorption of mixed anionic dyes in aqueous solution by magnetic NiFe-layered double oxide

Sonchai Intachai,Pornnapa Tongchoo,Panita Sumanatrakul,Prakaidao Pankam,Nithima Khaorapapong 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.10

The present research reports the adsorption capacity of magnetic NiFe-layered double oxide (NiFe-LDO) derived from the heat treatment of NiFe-layered double hydroxide (NiFe-LDH) on capturing of single anionic dye and mixed dyes in water. The formation and characteristics of NiFe-LDH and NiFe-LDO were verified by XRD, FT-IR, SEM, BET, VSM and UV-VIS. The obtained NiFe-LDO was mainly composed of NiFe2O4 and NiO phases, which showed strong magnetic property and made the adsorbents easily collected by external magnet. The amounts of the anionic dyes were captured on NiFe-LDO surface more than that on NiFe-LDH surface due to the larger surface area (68m2/g for NiFe-LDO and 50m2/g for NiFe-LDH). The maximum adsorption capacity of NiFe-LDO was in the range of 256-313mg/g and 244-300mg/g for eliminating the anionic dyes in single and multiple dye solution, respectively. The chemical adsorption affinity of the anionic dyes on the positive-charge surface of NiFe-LDO increased as follows: methyl orange<alizarin red S<orange II<Congo red. This work shows that NiFe-LDO is an efficient and costeffective adsorbent for eliminating multiple anionic dyes and reusing for several times.

잘쯔만결절변성으로 오인된 각막표층의 아밀로이드증의 치료 1예

이석재(Seok Jae Lee),이재정(Jae Jung Lee),이종수(Jong Soo Lee) 대한검안학회 2015 Annals of optometry and contact lens Vol.14 No.4

Purpose: To report the clinical efficacy of amnionic membrane corneal graft combined with using grind ball in a case of secondary localized corneal amyloidosis as clinical misdiagnosed saltzmann nodule. Case summary: A 64-year-old man with intermittent ocular pain and decreased visual acuity in left eye visited our clinic. In the first visit, the best corrected visual acuity (BCVA) in left eye was finger count and ocular pressure in left eye was 15 mmHg. The neovascularization from temporal side of the limbus to central corneal nodules was observed and was formed to a pannus. He had surgery on corneal lesion, removed corneal nodules by superficial keratectomy and trimmed the irregular corneal surface using grind ball and then transplanted amniotic membrane. A removed corneal tissue of the lesion was examined by immnohistochemistry and the tissue was stained positively with Congo red. No systemic involvement was noted form systemic work-up. Secondary localized amyloidosis of the cornea was diagnosed. After surgery for 3 month, the BCVA was 20/100 in left eye and there was no complications and recurrence on the corneal lesion at 11 month after surgery. Conclusions: It should be considered as one of useful therapeutic technique for management of superficial corneal amyloidosis by removing the lesion through superficial keratectomy, and then trimming and smoothing the irregular corneal surface using grind ball and transplanting the amniotic membrane.