오가피 열매 발효주의 제조 및 특성

최재명,김광엽,이상화,안준배 한국산업식품공학회 2010 산업 식품공학 Vol.14 No.1

본 연구에서는 오가피(Acanthopanax sessiliflorus)의 열매 발효주를 제조하였고 발효 중 생리활성 성분의 함량 변화를 측정함으로써 그 특징을 규명하였다. 열매의 일반성분 조성은 수분75.74±0.49%(w/w), 조단백질 12.51±1.23%(w/w), 조지방 4.20±0.51.%(w/w), 조회분 5.21±1.64%(w/w)이었으며 주된 미네랄 성분은 칼륨(12.94±0.08 mg/g), 칼슘(1.53±0.06 mg/g), 마그네슘(1.12±0.05 mg/g) 등 이었다. 발효주 제조를 위해 설탕으로 24, 26, 28, 30, 32oBrix가 되도록 가당하고 발효 한 결과 30°Brix 이상 가당 하였을 경우 알콜 발효가 저해되었으므로 목적하는 최종 알콜 농도와 잔당에 따라 24-30oBrix가 되도록 조절하는 것이 바람직하였다. 발효가 진행되면서 총 폴리페놀의 함량은 증가하는 경향을 보였고 20oC로 발효한 경우가 125.24±1.86 mg/mL로 25oC(99.69±2.11 mg/mL), 30oC(95.55±1.54 mg/mL)에서 발효한 경우보다 더 많은 총 폴리페놀 함량을 보였다. 또한 전자공여능을 측정 한 결과 20, 25, 30oC에서 발효 시 각각 85.9±2.3, 55.7±2.5, 55.2±3.4%로 20oC에서 발효하였을 경우 가장 높은 전자공여능을 보였는데 이는 1%(w/v) α-tocopherol의 전자공여능과 비슷한 수준이었다. 오가피 열매의 주된 생리활성 물질인 eleutheroside B 함량은 발효가 진행됨에 따라 증가하여 최대 146.58±4.10 μg/mL에 이르렀다. 그러나 발효 온도에 따라 eleutheroside B 함량은 차이가 없음을 알 수 있었다. 본 연구를 통해 착색료나 동물사료로 사용되거나 폐기되는 오가피 열매가 생리활성 물질을 다량 함유한 고부가가치 기능성 식품 소재로 활용될 수 있는 가능성을 제시하였다. Fruit wine from Acanthopanax sessiliflorus(A. sessiliflorus) including many pharmacological components was manufactured and its functional properties were investigated. The fruit part of A. sessiliflorus contained 75.74±0.49%(w/w) moisture, 12.51±1.23%(w/w) crude protein, 4.20±0.51.%(w/w) crude fat and 5.21±1.64%(w/w) crude ash. Minerals of fruit were potassium(12.94±0.08 mg/g), calcium(1.53±0.06 mg/g) and magnesium(1.12±0.05 mg/g). Initial soluble solid and fermentation temperature were 24-30oBrix and 20oC for manufacturing fruit wine from A. sessiliflorus. When initial soluble solid of a must was adjusted to more than 30°Brix, ethanol production was suppressed slightly. The polyphenol content of the fruit wine fermented at 20oC(125.24±1.86 mg/mL) was higher than those at 25oC(99.69±2.11 mg/mL) and 30oC(95.55±1.54 mg/mL). Electron donating activities of wines fermented at 20, 25 and 30oC were 85.9±2.3, 55.7±2.5 and 55.2±3.4%, respectively. The content of eleutheroside B increased up to 146.58±4.10 μg/mL during fermentation. There was no significant effect of fermentation temperature on eleutheroside B content. The fruit part of A. sessiliflorus can be used as a valuable resource for development of nutraceutical foods.

오가피 열매 발효주의 제조 및 특성

최재명 ( Jae Myoung Choi ),김광엽 ( Kwang Yup Kim ),이상화 ( Sang Hwa Lee ),안준배 ( Jun Bae Ahn ) 한국산업식품공학회 2010 산업 식품공학 Vol.14 No.1

본 연구에서는 오가피(Acanthopanax sessiliflorus)의 열매 발효주를 제조하였고 발효 중 생리활성 성분의 함량 변화를 측정함으로써 그 특징을 규명하였다. 열매의 일반성분 조성은 수분75.74±0.49%(w/w), 조단백질 12.51±1.23%(w/w), 조지방 4.20±0.51.%(w/w), 조회분 5.21±1.64%(w/w)이었으며 주된 미네랄 성분은 칼륨(12.94±0.08 mg/g), 칼슘(1.53±0.06 mg/g), 마그네슘(1.12±0.05 mg/g) 등 이었다. 발효주 제조를 위해 설탕으로 24, 26, 28, 30, 32˚Brix가 되도록 가당하고 발효 한 결과 30°Brix 이상 가당 하였을 경우 알콜 발효가 저해되었으므로 목적하는 최종 알콜 농도와 잔당에 따라 24-30˚Brix가 되도록 조절하는 것이 바람직하였다. 발효가 진행되면서 총 폴리페놀의 함량은 증가하는 경향을 보였고 20˚C로 발효한 경우가 125.24±1.86 mg/mL로 25˚C(99.69±2.11 mg/mL), 30˚C(95.55±1.54 mg/mL)에서 발효한 경우보다 더 많은 총 폴리페놀 함량을 보였다. 또한 전자공여능을 측정 한 결과 20, 25, 30˚C에서 발효시 각각 85.9±2.3, 55.7±2.5, 55.2±3.4%로 20˚C에서 발효하였을 경우 가장 높은 전자공여능을 보였는데 이는 1%(w/v) α-tocopherol의 전자공여능과 비슷한 수준이었다. 오가피 열매의 주된 생리활성 물질인 eleutheroside B 함량은 발효가 진행됨에 따라 증가하여 최대 146.58±4.10 μg/mL에 이르렀다. 그러나 발효 온도에 따라 eleutheroside B 함량은 차이가 없음을 알 수 있었다. 본 연구를 통해 착색료나 동물사료로 사용되거나 폐기되는 오가피 열매가 생리활성 물질을 다량 함유한 고부가가치 기능성 식품 소재로 활용될 수 있는 가능성을 제시하였다. Fruit wine from Acanthopanax sessiliflorus(A. sessiliflorus) including many pharmacological components was manufactured and its functional properties were investigated. The fruit part of A. sessiliflorus contained 75.74±0.49%(w/w) moisture, 12.51±1.23%(w/w) crude protein, 4.20±0.51.%(w/w) crude fat and 5.21±1.64%(w/w) crude ash. Minerals of fruit were potassium(12.94±0.08 mg/g), calcium(1.53±0.06 mg/g) and magnesium(1.12±0.05 mg/g). Initial soluble solid and fermentation temperature were 24-30˚Brix and 20˚C for manufacturing fruit wine from A. sessiliflorus. When initial soluble solid of a must was adjusted to more than 30°Brix, ethanol production was suppressed slightly. The polyphenol content of the fruit wine fermented at 20˚C(125.24±1.86 mg/mL) was higher than those at 25˚C(99.69±2.11 mg/mL) and 30˚C(95.55±1.54 mg/mL). Electron donating activities of wines fermented at 20, 25 and 30˚C were 85.9±2.3, 55.7±2.5 and 55.2±3.4%, respectively. The content of eleutheroside B increased up to 146.58±4.10 μg/mL during fermentation. There was no significant effect of fermentation temperature on eleutheroside B content. The fruit part of A. sessiliflorus can be used as a valuable resource for development of nutraceutical foods.

오가피의 기능성 물질 추출조건에 관한 연구

안준배,이상화,최재명 서원대학교 미래창조연구소 2011 과학과 문화 Vol.8 No.1

Acanthopanax sessiliflorus(A. sessiliflorus) has been known as traditional medicine having anti-stress, antioxidative and platelet aggregation inhibitory effects. Eleutheroside E is the standard substance showing functional properties in A. sessiliflorus. This study was constituted to establish the appropriate extraction conditions(extraction solvent, time, temperature and the number of extraction time) of eleutheroside E from A. sessiliflorus. 50%(v/v) methanol(methanol : water = 1:1) and distilled water were appropriate solvents for extraction. The appropriate extraction temperature and time turned out to be 80℃, 3hours, respectively. Due to heat unstability of eleutheroside E, exposure at high temperature(above 80℃) for a long time induced the degradation of eleutheroside E. Therefore one time extraction was advantageous to obtain eleutheroside E from A. sessiliflorus. This results can be used for development of nutraceutical foods and pharmaceuticals.

오갈피나무의 부위별 Eleutheroside B와 Eleutheroside E의 정량

이상현,강삼식,조선행,류수노,이범종 한국생약학회 2005 생약학회지 Vol.36 No.2

High performance liquid chromatography (HPLC) was used for the determination of eleutherosides B and E in various parts of Acanthopanax species. Eleutheroside B was detected in the stem (1.45 mg/g) and the root (0.59 mg/g) of A. senticosus and the stem (0.31 mg/g) of A. senticosus grafted on A. sessiliflorus. But there is no detection of eleutheroside B in A. sessiliflorus and the root of A. senticosus grafted on A. sessiliflorus. Eleutheroside E was detected in the stem (0.92, 0.97 and 0.30 mg/g, respectively) and the root (0.65, 0.94, 0.34 mg/g, respectively) of A. senticosus, A. sessiliflorus and A. senticosus grafted on A. sessiliflorus.

오가피 부위별 열수 추출액의 기능적 특성

최재명 ( Jae Myoung Choi ),김광엽 ( Kwang Yup Kim ),이상화 ( Sang Hwa Lee ),안준배 ( Jun Bae Ahn ) 한국산업식품공학회 2011 산업 식품공학 Vol.15 No.2

Acanthopanax sessiliflorus (A. sessiliflorus) has been known as a traditional medicine having anti-stress, antioxidative and platelet aggregation inhibitory effects. This study was undertaken to investigate the functional properties of water extracts from four parts of A. sessiliflorus. Root, stem, leaf and fruit extracts from A. sessiliflorus were prepared with hot water (80℃). The contents of functional substances, eleutheroside B and E, polyphenol, antioxidative activity, nitrite scavenging ability and anti-cancer activity of the extracts were determined. The contents of eleutheroside E in stem, root and fruit extracts were 542.50 μg/g, 343.35 μg/g and 30.78 μg/g, respectively. A large part of eleutheroside B was found in fruit (372.01 μg/g) and root (289.33 μg/g) extracts. Root and stem extracts contained 227.21 mg/100g and 131.22 mg/100g of polyphenols, respectively. Antioxidative activities (electron donating ability) of stem and root extracts were 79.87% and 77.27%, respectively. It appears that the antioxidative activities were related to polyphenol contents of the extracts. Most extracts showed 76-81.5% of nitrite scavenging ability at pH 1.2. It reveals that water extract from parts of A. sessiliflorus can inhibit formation of nitrosoamine in food. Effects of the extracts on the growth of normal and cancer cell lines were investigated. Extracts showed no cytotoxicity to normal dendritic cell line (DC2.4). Especially, the root extract promoted the growth of normal cell line. Root and stem extracts had 20-23% of inhibitory effect against stomach cancer cell line (SNU-719) and liver cancer cell line (Hep3B). These result indicated that the extracts from A. sessiliflorus can be used as functional food materials with antioxidative activity and nitrite scavenging ability to eliminate nitrosoamine in food.

HPLC에 의한 가시오가피 및 오가피 중 Hyperin의 정량

이상현,정하숙,신국현,김박광 대한약학회 2004 약학회지 Vol.48 No.4

A new high performance liquid chromatographic method was applied for the determination of hyperin in Acanthopanax senticosus and A sessiliflorus. The stationary phase used was $\beta$-Bondapak $C_{18}$ reverse-phase column and a mobile phase program was a gradient of acetonitrile and distilled water at a flow rate of 1.0 ml/min. Hyperin was detected at 210 nm, and the analysis was successfully carried out within 20 min. Hyperin was detected in the one year-grown and two years-grown stem of A senticosus (0.47 and 0.13 mg/g, respectively) and A sessiliflorus (0.14 and 0.03 mg/g, respectively). Hyperin was detected in the main and branch root of A sessiliflorus (0.30 and 0.09 mg/g, respectively). But there is no detection of hyperin in the main and branch root of A senticosus.

오가피 부위별 열수 추출액의 기능적 특성

최재명,김광엽,이상화,안준배 한국산업식품공학회 2011 산업 식품공학 Vol.15 No.2

오가피는 다양한 생리활성을 가진 약재로 사용되어왔다. 본 연구에서는 오가피 부위별 열수 추출액의 기능적 특징을 규명하기위해 추출부위별 총 폴리페놀 함량, 항산화활성, 아질산염 소거능 및 항암활성을 알아보았다. 오가피의 생리활성 표준물질인 eleutheroside E는 줄기(542.50 μg/g) >뿌리 (343.35 μg/g) >열매(30.78 μg/g) 순으로 함유되어 있었고 eleutheroside B는 열매(372.01 μg/g) >뿌리(289.33 μg/g) >줄기(125.05 μg/g) 순으로 많이 함유되어 있었다. 총 폴리페놀은 뿌리(227.21 mg/100 g)와 줄기(131.22 mg/100 g)에 많이 함유되어 있었다. 전자공여능은 줄기에서 79.87%, 뿌리에서 77.27%를 보여 총 폴리페놀 함량과 상관관계가 있었다. 산성 환경에서 nitrosoamine을 생성하는 아질산염에 대한 오가피의 제거 효과는 추출부위와 관계없이 pH 1.2에서 76-81.5%로 높게 나타났다. pH가 높아짐에따라 아질산염제거효과는 대개 소실되었으나 열매 추출액의 경우 pH 6.0에서도 43.1%의 아질산염제거효과를 유지하고 있었다. 오가피 부위별 열수 추출액은 정상세포인 DC2.4의 생육 억제 효과는 관찰되지 않았고 오히려 뿌리추출액의 경우는 DC2.4의 생육을 유의미하게 촉진하는 것으로 밝혀져 세포독성은 없는 것으로 판단되었다. 위암 세포주인 SNU-719와 간암세포인 Hep3B에 대해서는 뿌리 추출액과 줄기 추출액에서 20-23%의 억제효과를 보였다. 따라서 오가피 부위별 추출액은 항산화활성, 식육에서의 nitrosoamine 생성 억제 등 다양한 기능성을 가진 식품소재로 활용이 가능 할 것으로 판단되었다. Acanthopanax sessiliflorus (A. sessiliflorus) has been known as a traditional medicine having anti-stress, antioxidative and platelet aggregation inhibitory effects. This study was undertaken to investigate the functional properties of water extracts from four parts of A. sessiliflorus. Root, stem, leaf and fruit extracts from A. sessiliflorus were prepared with hot water (80oC). The contents of functional substances, eleutheroside B and E, polyphenol, antioxidative activity, nitrite scavenging ability and anti-cancer activity of the extracts were determined. The contents of eleutheroside E in stem, root and fruit extracts were 542.50 μg/g, 343.35 μg/g and 30.78 μg/g, respectively. A large part of eleutheroside B was found in fruit (372.01 μg/g) and root (289.33 μg/g) extracts. Root and stem extracts contained 227.21 mg/100g and 131.22 mg/100g of polyphenols, respectively. Antioxidative activities (electron donating ability) of stem and root extracts were 79.87% and 77.27%, respectively. It appears that the antioxidative activities were related to polyphenol contents of the extracts. Most extracts showed 76-81.5% of nitrite scavenging ability at pH 1.2. It reveals that water extract from parts of A. sessiliflorus can inhibit formation of nitrosoamine in food. Effects of the extracts on the growth of normal and cancer cell lines were investigated. Extracts showed no cytotoxicity to normal dendritic cell line (DC2.4). Especially, the root extract promoted the growth of normal cell line. Root and stem extracts had 20-23% of inhibitory effect against stomach cancer cell line (SNU-719) and liver cancer cell line (Hep3B). These result indicated that the extracts from A. sessiliflorus can be used as functional food materials with antioxidative activity and nitrite scavenging ability to eliminate nitrosoamine in food.

Chemical Characteristics and Immuno-stimulating Properties of Biopolymers Extracted from Acanthopanax sessiliflorus

Jeong, Sang-Chul,Jeong, Young-Tae,Yang, Byung-Keun,Song, Chi-Hyun Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.1

During our search for macrophage stimulating compounds from medicinal plants, we isolated biopolymers from Acanthopanax sessiliflorus. Isolated fraction AS-5 showed maximum potential, and stimulated lysosonal enzymatic activity by 230% at $300\;{\mu}g/ml$. The nitric oxide (NO) producing ability of AS-5 $100\;{\mu}g/ml$ was $58\;{\mu}M$ when treated with interferon-$\gamma$ and lipopolysaccharide $20\;{\mu}g/ml$. The lymphocyte proliferating effects of isolated biopolymer fractions were also investigated. Highest lymphoproliferative activity (a 2.8-fold enhancement compared to saline treated group was exhibited by AS-3 at $200\;{\mu}g/ml$ followed by AS-5 and AS-6. The AS-3 fraction stimulated only T-lymphocytes and had little or no effect on B-lymphocyte proliferation. Partially methylated alditol acetates were prepared to elucidate the glycosyl linkage-compositions of the AS-3 and AS-5 biopolymers, and were analyzed by GC-MS. The AS-3 and AS-5 biopolymer fractions were found to contain 2,3,4-tri-O-methyl-D-glucitol, 2,3,4-tri-O-methyl-D-galacitol 3,4,6-tri-O-methyl-galacitol, 2-O-methyl-arabinitol and 2,4,6-tri-O-methyl-D-glucitol, 2,3,6-tri-O-methyl-D-galacitol linkages, respectively.

효소적 생물전환을 이용한 토종오가피와 가시오가피의 Eleutheroside B, E의 추출 함량 최적화

김나리(Na-Ri Kim),박종순(Jong-Soon Park),이득식(Deuk-Sik Lee),심재훈(Jae-Hoon Shim) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.9

본 연구에서는 효소적 생물전환을 이용하여 오가피의 생리 활성 물질인 eleutheroside B와 E의 추출 함량을 증대시키는 방안을 모색하였다. 효율적인 열수추출 공정을 위해 줄기 분말에 질량대비 가수를 한 결과 14배 가수를 선정하였다. 이후 줄기 분말에 다양한 가수분해 효소를 이용하여 처리한 결과 유효성분의 용출량을 증가시키는 효소로 Novozyme 33095, Viscozyme L을 선정하였다. 효소처리 시간별로 유효성분의 용출량을 비교한 결과 eleutheroside B와 E의 용출량은 가시오가피 줄기에 Novozyme 33095로 3시간 처리하였을 경우 eleutheroside B의 용출량이 대조구 대비 약 13%가 증가하였고, eleutheroside E의 용출량은 대조구 대비 약 26%로 가장 많이 증가하였다. 효소처리 후 토종오가피와 가시오가피의 줄기의 효과적인 열수추출 조건을 확인해 본 결과 토종오가피를 121°C에서 15분 동안 추출하였을 때 eleutheroside B의 추출 함량은 기존 열수추출 공정대비 약 7%가 증가하였고, eleutheroside E의 추출 함량은 약 17%가 유의적으로 증가하였다. 가시오가피 줄기를 121°C에서 15분 동안 추출하였을 때 eleutheroside B의 추출 함량은 기존 열수추출 공정대비 약 25%가 유의적으로 증가하였고, eleutheroside E의 추출 함량은 약 29%가 유의적으로 증가하였다. In this study, we optimized conditions for extraction of eleutherosides B and E from stem powder of Acanthopanax. To enhance eleutheroside B and E yields, Acanthopanax sessiliflorus Seeman and Eleutherococcus senticosus Maxim were pre-incubated with the following enzymes: Celluclast, Viscozyme L, Lactozyme, Lecitase, and Novozyme 33095. Treatment with Novozyme 33095, a commercial pectinase, for 3 h resulted in the highest yields of eleutherosides B and E from A. sessiliflorus and E. senticosus. Compared with extraction at 121°C for 120 min, at 121°C for 15 min after Novozyme 33095 pre-incubation increased eleutheroside B and E yields from A. sessiliflorus by 7% and 17%, respectively. In the case of E. senticosus, eleutheroside B and E yields increased by 25% and 29%, respectively.

韓藥 吾加皮의 生藥學的 硏究

박종희,김진수,이준도 부산대학교 신약개발연구소 1997 藥學硏究誌 Vol.31 No.1

"O Ga Pi" is one of the Chinese crude drugs used mainly to cure arthritis, acute gastritis and beriberi. With regard to the botanical origin of "O Ga Pi", it has been considered to be Acanthopanax species of Araliaceae, but there has no pharmacognostical confirmation on it. To clarify the botanical origin of "O Ga Pi", the anatomical characteristics of the branch of Acanthopanax species growing wildly in Korea, A. chiisanensis Nakai, A. koreanum Nakai, A. senticosus (Rupr. et Max.) Harms, A senticosus (Rupr. et Max.) Harms var. inermis Harms and A. sessiliflorus (Rupr. et Max.) Seem. were studied. As a result, it was clarified that "O Ga Pi" was the branch, rarely the bark of branch of Acamthopanax sessiliflorus.