An Enhancer Mutant of Arabidopsis salt overly sensitive 3 Mediates both Ion Homeostasis and the Oxidative Stress Response

Zhu, Jianhua,Fu, Xinmiao,Koo, Yoon Duck,Zhu, Jian-Kang,Jenney Jr., Francis E.,Adams, Michael W. W.,Zhu, Yanmei,Shi, Huazhong,Yun, Dae-Jin,Hasegawa, Paul M.,Bressan, Ray A. American Society for Microbiology 2007 Molecular and cellular biology Vol.27 No.14

<B>ABSTRACT</B><P>The myristoylated calcium sensor SOS3 and its interacting protein kinase, SOS2, play critical regulatory roles in salt tolerance. Mutations in either of these proteins render <I>Arabidopsis thaliana</I> plants hypersensitive to salt stress. We report here the isolation and characterization of a mutant called <I>enh1-1</I> that enhances the salt sensitivity of <I>sos3-1</I> and also causes increased salt sensitivity by itself. <I>ENH1</I> encodes a chloroplast-localized protein with a PDZ domain at the N-terminal region and a rubredoxin domain in the C-terminal part. Rubredoxins are known to be involved in the reduction of superoxide in some anaerobic bacteria. The <I>enh1-1</I> mutation causes enhanced accumulation of reactive oxygen species (ROS), particularly under salt stress. ROS also accumulate to higher levels in <I>sos2-1</I> but not in <I>sos3-1</I> mutants. The <I>enh1-1</I> mutation does not enhance <I>sos2-1</I> phenotypes. Also, <I>enh1-1</I> and <I>sos2-1</I> mutants, but not <I>sos3-1</I> mutants, show increased sensitivity to oxidative stress. These results indicate that ENH1 functions in the detoxification of reactive oxygen species resulting from salt stress by participating in a new salt tolerance pathway that may involve SOS2 but not SOS3.</P>

Involvement of Arabidopsis HOS15 in histone deacetylation and cold tolerance.

Zhu, Jianhua,Jeong, Jae Cheol,Zhu, Yanmei,Sokolchik, Irina,Miyazaki, Saori,Zhu, Jian-Kang,Hasegawa, Paul M,Bohnert, Hans J,Shi, Huazhong,Yun, Dae-Jin,Bressan, Ray A National Academy of Sciences 2008 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.105 No.12

<P>Histone modification in chromatin is one of the key control points in gene regulation in eukaryotic cells. Protein complexes composed of histone acetyltransferase or deacetylase, WD40 repeat protein, and many other components have been implicated in this process. Here, we report the identification and functional characterization of HOS15, a WD40-repeat protein crucial for repression of genes associated with abiotic stress tolerance through histone deacetylation in Arabidopsis. HOS15 shares high sequence similarity with human transducin-beta like protein (TBL), a component of a repressor protein complex involved in histone deacetylation. Mutation of the HOS15 gene renders mutant plants hypersensitive to freezing temperatures. HOS15 is localized in the nucleus and specifically interacts with histone H4. The level of acetylated histone H4 is higher in the hos15 mutant than in WT plants. Moreover, the stress inducible RD29A promoter is hyperinduced and associated with a substantially higher level of acetylated histone H4 in the hos15 mutant under cold stress conditions. Our results suggest a critical role for gene activation/repression by histone acetylation/deacetylation in plant acclimation and tolerance to cold stress.</P>

Local Field Enhancement Due to Multiple Scattering in Random Sb-SiN Thin Films

Ruo-Jian Zhu,Ding-Rong Ou,Guo-Fan Jin,Jia Wang,Jing Zhu 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.47 No.1

Random Sb-SiN films consisting of random small Sb particles embedded in a SiN thin film were deposited by radio-frequency magnetron sputtering. Specimens comprising in order a random Sb- SiN film and an optical-phase-change recording layer were exposed to a focused laser beam. It was shown that, with a random Sb-SiN layer deposited above the recording layer, the ablation of the recording layer occurred much faster and under much lower power than that of a single recording layer. Scanning Near-field Optical Microscope (SNOM) images of the transmitted light after passing through the random Sb-SiN film were produced as well as a Finite Difference Time Domain (FDTD) calculation of the field distribution. They both showed a local optical near-field enhancement due to multiple scattering effects of the Sb nanoparticles. This is promising for application in superresolution optical storage to get a higher Carrier to Noise Ratio (CNR).

Low Temperature Heat Assisted Magnetization Reversal and Its Application to MRAM

Jian-Gang (Jimmy) Zhu,Yi Luo 한국자기학회 2008 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Hath1 Inhibits Proliferation of Colon Cancer Cells Probably Through Up-regulating Expression of Muc2 and p27 and Down-regulating Expression of Cyclin D1

Zhu, Dai-Hua,Niu, Bai-Lin,Du, Hui-Min,Ren, Ke,Sun, Jian-Ming,Gong, Jian-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Previous studies showed that Math1 homologous to human Hath1 can cause mouse goblet cells to differentiate. In this context it is important that the majority of colon cancers have few goblet cells. In the present study, the potential role of Hath1 in colon carcinogenesis was investigated. Sections of paraffin-embedded tissues were used to investigate the goblet cell population of normal colon mucosa, mucosa adjacent colon cancer and colon cancer samples from 48 patients. Hath1 and Muc2 expression in these samples were tested by immunohistochemistry, quantitative real-time reverse transcription -PCR and Western blotting. After the recombinant plasmid, pcDNA3.1(+)-Hath1 had been transfected into HT29 colon cancer cells, three clones were selected randomly to test the levels of Hath1 mRNA, Muc2 mRNA, Hath1, Muc2, cyclin D1 and p27 by quantitative real-time reverse transcription-PCR and Western blotting. Moreover, the proliferative ability of HT29 cells introduced with Hath1 was assessed by means of colony formation assay and xenografting. Expression of Hath1, Muc2, cyclin D1 and p27 in the xenograft tumors was also detected by Western blotting. No goblet cells were to be found in colon cancer and levels of Hath1 mRNA and Hath1, Muc2 mRNA and Muc2 were significantly down-regulated. Hath1 could decrease cyclin D1, increase p27 and Muc2 in HT29 cells and inhibit their proliferation. Hath1 may be an anti-oncogene in colon carcinogenesis.

Comparative Study of DC Breakdown and Space Charge Characteristics of Insulation Paper Impregnated with Natural Ester and Mineral Oil

Jian Hao,Run-Hao Zou,Rui-Jin Liao,Li-Jun Yang,Qiang Liao,Meng-Zhao Zhu 대한전기학회 2018 Journal of Electrical Engineering & Technology Vol.13 No.4

Natural ester is a suitable substitute for mineral oil and has been widely used in AC transformer in many countries. In order to further application of natural ester in direct current (DC) equipment, it is needed to investigate its long term insulation property under DC condition. In this paper, a thermal ageing experiment was conducted for both mineral oil-paper and natural ester-paper insulation. The DC breakdown and space charge characteristics of insulation paper impregnated with natural ester and mineral oil was compared. Results show that the resistivity of the paper immersed in natural ester and mineral oil both increase as the ageing goes on. While insulation paper impregnated with natural ester has higher resistivity and DC breakdown voltage than the paper impregnated with mineral oil. The DC breakdown voltage for the oil impregnated insulation paper being DC prestressing is higher than that without pre-stressing. The average DC breakdown field strength difference between the test with pre-stressing and without pre-stressing clearly shows that there is an apparent enhancement effect for the homo-charge injection on the DC breakdown.

An Effective Guidewire Looping Technique for the Recanalization of Occlusive Segments of Infrapopliteal Vessels

Jian-bo Wang,Jun-gong Zhao,Ming-hua Li,Yue-qi Zhu,Jue Wang,Pei-lei Zhang 대한영상의학회 2010 Korean Journal of Radiology Vol.11 No.4

Objective: To determine the efficacy, safety and primary follow-up results of a guidewire looping technique for the treatment of infrapopliteal arteries. Materials and Methods: From October 2006 to May 2008, an intraluminal angioplasty of the infrapopliteal arteries was attempted in 200 consecutive patients. Altogether, 417 infrapopliteal lesions, with lengths varying from 2 cm to 32 cm, were treated as part of this study, including 305 lesions in the anterior tibial arteries, 89 in the posterior tibial arteries, and 23 in the peroneal arteries. The ‘U’-shaped guidewire technique was attempted in 393 lesions from 361 limbs. The tip of a hydrophilic 0.035-inch guidewire was formed into a ‘U’ shape with the aid of a 4-Fr catheter and collateral branch vessel to recanalize the completely occluded long segment lesions. Results: A successful angioplasty with at least one artery recanalized directly to the malleolar or dorsal foot was achieved in 322 limbs (89%). The looping technique had a success rate of 90% (352 of 393 lesions). After the procedure, the rest pain was relieved in 58 of 69 patients, while 207 of 245 limbs (85%) showed improvement for intermittent claudication. Complete wound healing was noted in 21 of 54 patients, while 20 of 54 patients showed an improvement in the wound size or depth. A total of 38 major immediate procedure-related complications were noted, including retroperitoneal hematoma, distal emboli, and vessel rupture. Conclusion: The results of this study suggests that the guidewire looping technique is a safe and effective method for the recanalization of the occluded lesions in infrapopliteal vessels. Objective: To determine the efficacy, safety and primary follow-up results of a guidewire looping technique for the treatment of infrapopliteal arteries. Materials and Methods: From October 2006 to May 2008, an intraluminal angioplasty of the infrapopliteal arteries was attempted in 200 consecutive patients. Altogether, 417 infrapopliteal lesions, with lengths varying from 2 cm to 32 cm, were treated as part of this study, including 305 lesions in the anterior tibial arteries, 89 in the posterior tibial arteries, and 23 in the peroneal arteries. The ‘U’-shaped guidewire technique was attempted in 393 lesions from 361 limbs. The tip of a hydrophilic 0.035-inch guidewire was formed into a ‘U’ shape with the aid of a 4-Fr catheter and collateral branch vessel to recanalize the completely occluded long segment lesions. Results: A successful angioplasty with at least one artery recanalized directly to the malleolar or dorsal foot was achieved in 322 limbs (89%). The looping technique had a success rate of 90% (352 of 393 lesions). After the procedure, the rest pain was relieved in 58 of 69 patients, while 207 of 245 limbs (85%) showed improvement for intermittent claudication. Complete wound healing was noted in 21 of 54 patients, while 20 of 54 patients showed an improvement in the wound size or depth. A total of 38 major immediate procedure-related complications were noted, including retroperitoneal hematoma, distal emboli, and vessel rupture. Conclusion: The results of this study suggests that the guidewire looping technique is a safe and effective method for the recanalization of the occluded lesions in infrapopliteal vessels.

End-to-end system level modeling and simulation for medium-voltage DC electric ship power systems

Zhu, Wanlu,Shi, Jian,Abdelwahed, Sherif The Society of Naval Architects of Korea 2018 International Journal of Naval Architecture and Oc Vol.10 No.1

Dynamic simulation is critical for electrical ship studies as it obtains the necessary information to capture and characterize system performance over the range of system operations and dynamic events such as disturbances or contingencies. However, modeling and simulation of the interactive electrical and mechanical dynamics involves setting up and solving system equations in time-domain that is typically time consuming and computationally expensive. Accurate assessment of system dynamic behaviors of interest without excessive computational overhead has become a serious concern and challenge for practical application of electrical ship design, analysis, optimization and control. This paper aims to develop a systematic approach to classify the sophisticated dynamic phenomenon encountered in electrical ship modeling and simulation practices based on the design intention and the time scale of interest. Then a novel, comprehensive, coherent, and end-to-end mathematical modeling and simulation approach has been developed for the latest Medium Voltage Direct Current (MVDC) Shipboard Power System (SPS) with the objective to effectively and efficiently capture the system behavior for ship-wide system-level studies. The accuracy and computation efficiency of the proposed approach has been evaluated and validated within the time frame of interest in the cast studies. The significance and the potential application of the proposed modeling and simulation approach are also discussed.

Interfacial performance enhancement of carbon fiber/epoxy composites by a twostep surface treatment

Jian Shi,Yuji Yamamoto,Mamoru Mizuno,Chunhong Zhu 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.1

Carbon fiber-reinforced polymer composites (CFRPs) have many features, such as lightweight, high specific strength, and excellent chemical resistance. However, the adhesion at the interface of the CFRPs, which are composed of fiber and resin, is poor. Thus, delamination and interfacial peeling occur, and the mechanical properties, such as intrinsic strength and rigidity, decrease. Fiber surface modification by plasma and silane coupling treatments has attracted much attention to improve these properties. In this study, argon and oxygen mixed gas plasma treatment was used as pretreatment to introduce hydroxyl group on the fiber surface. This method was utilized to improve the adhesion between the fiber and resin. A silane coupling treatment was conducted to bond the carbon fiber and epoxy resin by chemical covalent bonding. The effect of silane coupling treatment on the interfacial property of CFRPs was investigated. Elemental analysis of the surface modified carbon fiber was explored through energy dispersive X-ray spectrometry. The functional groups of different carbon fiber surfaces were analyzed through X-ray photoelectron spectroscopy. The wettability of the treated carbon fiber was examined with a contact angle meter. The interlaminar shear strength was determined with a short beam method.

Experimental and numerical investigation of uplift behavior of umbrella-shaped ground anchor

Zhu, Hong-Hu,Mei, Guo-Xiong,Xu, Min,Liu, Yi,Yin, Jian-Hua Techno-Press 2014 Geomechanics & engineering Vol.7 No.2

In the past decade, different types of underreamed ground anchors have been developed for substructures requiring uplift resistance. This article introduces a new type of umbrella-shaped anchor. The uplift behavior of this ground anchor in clay is studied through a series of laboratory and field uplift tests. The test results show that the umbrella-shaped anchor has higher uplift capacity than conventional anchors. The failure mode of the umbrella-shaped anchor in a large embedment depth can be characterized by an arc failure surface and the dimension of the plastic zone depends on the anchor diameter. The anchor diameter and embedment depth have significant influence on the uplift behavior. A finite element model is established to simulate the pullout of the ground anchor. A parametric study using this model is conducted to study the effects of the elastic modulus, cohesion, and friction angle of soils on the load-displacement relationship of the ground anchor. It is found that the larger the elastic modulus and the shear strength parameters, the higher the uplift capacity of the ground anchor. It is suggested that in engineering design, the soil with stiffer modulus and higher shear strength should be selected as the bearing stratum of this type of anchor.