Metastasis associated genomic aberrations in stage II rectal cancer

Hong Zhao,Zhi-Zhou Shi,Rui Jiang,Dong-Bing Zhao,Hai-Tao Zhou,Jian-Wei Liang,Xin-Yu Bi,Jian-Jun Zhao,Zhi-Yu Li,Jian-Guo Zhou,Zhen Huang,Ye-Fan Zhang,Jian Wang,Xin Xu,Yan Cai,Ming-Rong Wang,Yu Zhang 한국유전학회 2016 Genes & Genomics Vol.38 No.11

Genomic aberrations of rectal carcinoma, especially DNA copy number changes associated with metastasis were largely unclear. We aim to identify the metastasis associated biomarkers in stage II rectal cancer. Formalin-fixed, paraffin-embedded primary tumor tissues of stage II rectal carcinoma were analyzed by array-based comparative genomic hybridization, and genomic aberrations were identified by Genomic Workbench and SAM software. Copy number changes and mRNA expressions were validated by Real-time PCR in an independent rectal cancer samples. The results showed that the most frequent gains in stage II rectal cancer were at 1q21.2-q23.1, 3p21.31, 11q12.2-q23.3, 12q24.11-q24.31, 12q13.11-q14.1 and losses in 18q11.2-q23, 17q21.33-q22, 13q31.1-q31.3, 21q21.1-q21.3, 8p23.3-p23.1 and 4q22.1-q23. Twenty-two amplifications and five homozygous deletions were also identified. We further found that S100A1 (1q21.3-q23.1), MCM7 (7q22.1) and JUND (19p13.11) were amplified and overexpressed in stage II rectal cancer. Interestingly, the genomic aberrations affected 14 signaling pathways including VEGF signaling pathway and fatty acid metabolism. Most importantly, loss of 13q31.1-q34 and gain of 1q44 were associated with distant metastasis. Our results indicated that these metastasis associated genomic changes may be useful to reveal the pathogenesis of rectal cancer metastasis and identify candidate biomarkers.

A morphological study on autumnal morphs of Aphis glycines (Hemiptera: Aphididae)

Zhen-qi Tian,Su-ji Wang,Bing Bai,Jian Liu,Kuijun Zhao 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2

The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is one of the most important pests of soybean. The complex life cycle of A. glycines is characterized as heteroecious and holocyclic, and has seasonal polymorphisms occurring during its life cycle. In the autumn, A. glycines occur as winged and wingless virginoparae in soybeans, as gynoparae and males that migrate from soybeans to Rhamnus spp. and as gynoparae, males and oviparae on Rhamnus spp. In this study, wingless virginoparae, gynoparae, males and oviparae of A. glycines were successfully induced in the laboratory and morphological parameters of these morphs were selected for quantitation. To aid in identification of these A. glycines autumnal morphs, these aphids were imaged by microscope and significant differences in morphological characteristics were found: distal parts of hind leg femurs of gynoparae were grayish black, which were darker than those of winged virginoparae; the 4th instars of gynoparae, males and winged virginoparae differ greatly in dorsal abdomen coloration and covering. Our results provide an important guide for distinguishing adults of gynoparae and winged virginoparae, and for identifying nymphs of gynoparae, males and winged virginoparae.

Fluorescent Nanoparticles for Observing Primo Vascular System Along Sciatic Nerve

Zhao-Feng Jia,Ki-Hoon Eom,Jin-Myung Cha,Jin-Kyu Lee,Zhen-Dong Su,Wen-Hui Yu,Pan Dong Ryu,소광섭,Byung-Cheon Lee 사단법인약침학회 2010 Journal of Acupuncture & Meridian Studies Vol.3 No.3

The primo vascular system was found in the epineurium along the rat sciatic nerve following subcutaneous injection of fluorescent nanoparticles at the Zusanli acupoint (ST-36). Nanoparticles were injected into the primo-vessel near ST-36 and flowed along the sciatic nerve. Fluorescence revealed a structure in the epineurium that was hardly detectable. Images of the isolated sample stained with 4’,6-diamidino-2-phenylindole were captured using confocal microscopy. These images showed the distinctive nuclei distribution and multi-lumen structure of primo-vessels that differentiate them from lymphatic vessels, blood capillaries and nerves. This study demonstrates a new use for nanoparticles in fluorescence reflectance imaging techniques during in vivo imaging of primo-vessels.

An Efficient Revocable Group Signature Scheme in Vehicular Ad Hoc Networks

( Zhen Zhao ),( Jie Chen ),( Yueyu Zhang ),( Lanjun Dang ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.10

Although many revocable group signature schemes has been proposed in vehicular ad hoc networks (VANETs), the existing schemes suffer from long computation delay on revocation that they cannot adapt to the dynamic VANETs. Based on Chinese remainder theorem and Schnorr signature algorithm, this paper proposes an efficient revocable group signature scheme in VANETs. In the proposed scheme, it only need to update the corresponding group public key when a member quits the group, and in the meanwhile the key pairs of unchanged group members are not influenced. Furthermore, this scheme can achieve privacy protection by making use of blind certificates. Before joining to the VANETs, users register at local trusted agencies (LTAs) with their ID cards to obtain blind certificates. The blind certificate will be submitted to road-side units (RSUs) to verify the legality of users. Thus, the real identities of users can be protected. In addition, if there is a dispute, users can combine to submit open applications to RSUs against a disputed member. And LTAs can determine the real identity of the disputed member. Moreover, since the key pairs employed by a user are different in different groups, attackers are not able to track the movement of users with the obtained public keys in a group. Furthermore, performance analysis shows that proposed scheme has less computation cost than existing schemes.

Real-Time Contour-Error Estimation Methods for Three-Dimensional Free-Form Parametric Curves in Contour-Following Tasks

Zhen-yuan Jia,De-Ning Song,Jian-Wei Ma,Xiao-Xuan Zhao,Ning Zhang 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.2

It is crucial to control the contour error in curved contour-following tasks caused by reasons such as servo delay and external disturbance. Contour-error estimation plays as a precondition for its further control. Existing methods can hardly keep well estimation accuracy for high-speed following of free-form curves with sharp corners, especially for three-dimensional curves. Consequently, this paper presents three high-precision real-time contour-error estimation methods for spatial free-form parametric curved contour following. By generating and updating the backstepping point according to the tangential tracking error, a multiple tangential approximation method is presented first. Then, a spatial circular approximation method is given by means of approximating the actual-position nearby region of the desired contour with a spatial circle. Finally, via modification of the Newton method so as to improve its stability without sacrificing of its fast convergence property, an initial value regeneration-based Newton algorithm is proposed for contour-error estimation. All of the presented methods take both estimation precision and calculation burden into consideration, and possess their own advantages. Using these algorithms, the contour error can be rapidly estimated in vector form with a high accuracy. Simulation and experimental results demonstrate the feasibility and the superiority of the presented algorithms.

Semi-active control on long-span reticulated steel structures using MR dampers under multi-dimensional earthquake excitations

Zhen Zhou,Shao-ping Meng,Jing Wu,Yong Zhao 국제구조공학회 2012 Smart Structures and Systems, An International Jou Vol.10 No.6

This paper focuses on the vibration control of long-span reticulated steel structures under multidimensional earthquake excitation. The control system and strategy are constructed based on Magneto- Rheological (MR) dampers. The LQR and Hrovat controlling algorithm is adopted to determine optimal MR damping force, while the modified Bingham model (MBM) and inverse neural network (INN) is proposed to solve the real-time controlling current. Three typical long-span reticulated structural systems are detailedly analyzed, including the double-layer cylindrical reticulated shell, single-layer spherical reticulated shell, and cable suspended arch-truss structure. Results show that the proposed control strategy can reduce the displacement and acceleration effectively for three typical structural systems. The displacement control effect under the earthquake excitation with different PGA is similar, while for the cable suspended arch-truss, the acceleration control effect increase distinctly with the earthquake excitation intensity. Moreover, for the cable suspended arch-truss, the strand stress variation can also be effectively reduced by the MR dampers, which is very important for this kind of structure to ensure that the cable would not be destroyed or relaxed.

Cordycepin inhibits chondrocyte hypertrophy of mesenchymal stem cells through PI3K/Bapx1 and Notch signaling pathway

( Zhen Cao ),( Ce Dou ),( Jianmei Li ),( Xiangyu Tang ),( Junyu Xiang ),( Chunrong Zhao ),( Lingyu Zhu ),( Yun Bai ),( Qiang Xiang ),( Shiwu Dong ) 생화학분자생물학회(구 한국생화학분자생물학회) 2016 BMB Reports Vol.49 No.10

Mesenchymal stem cells (MSCs) are widely used in cartilage tissue engineering to repair articular cartilage defects. However, hypertrophy of chondrocytes derived from MSCs might hinder the stabilization of hyaline cartilage. Thus, it is very important to find a suitable way to maintain the chondrogenic phenotype of chondrocytes. It has been reported that cordycepin has anti-inflammatory and anti-tumor functions. However, the role of cordycepin in chondrocyte hypertrophy remains unclear. Therefore, the objective of this study was to determine the effect of cordycepin on chondrogenesis and chondrocyte hypertrophy in MSCs and ATDC5 cells. Cordycepin upregulated chondrogenic markers including Sox9 and collagen type II while down-regulated hypertrophic markers including Runx2 and collagen type X. Further exploration showed that cordycepin promoted chondrogenesis through inhibiting Nrf2 while activating BMP signaling. Besides, cordycepin suppressed chondrocyte hypertrophy through PI3K/Bapx1 pathway and Notch signaling. Our results indicated cordycepin had the potential to maintain chondrocyte phenotype and reconstruct engineered cartilage. [BMB Reports 2016; 49(10): 548-553]

Analysis of the Threats abusing IPv6 Fragment Header

( Zhen Zhao ),( Gyehyeon Gyeong ),( Kwang Sun Ko ),( Young Ik Eom ) 한국정보처리학회 2007 한국정보처리학회 학술대회논문집 Vol.14 No.1

The security issues related to IPv6 protocol have been focused on by many researchers and engineers. Especially, extension headers of IPv6 protocol provide various functionalities such as IP security, mobile IP, and in principle, it is said to give much more effective network services than the previous protocol, IPv4. In this paper, the cases are surveyed in which fragment header, that is one of many extension headers in IPv6 protocol, is abusedand made to be the sources of threats. Prevention mechanisms are also surveyed to countermeasure the threats.

Longitudinal anti-cracking analysis for post-tensioned voided slab bridges

Zhen Zhou,Shao-ping Meng,Zhao Liu 국제구조공학회 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.43 No.4

Post-tensioned concrete voided slab girders are widely used in highway bridge constructions. To obtain greater section hollow rate and reduce the self-weight, the plate thickness of slab girders are designed to be small with the adoption of flat anchorage system. Since large prestress is applied to the anchor end section, it was found that longitudinal shear cracks are easy to occur along the voided slab girder. The reason is the existence of great shearing effect at the junction area between web and bottom (top) plate in the anchor end section. This paper focuses on the longitudinal anti-cracking problem at the anchor end of post-tensioned concrete voided slab girders. Two possible models for longitudinal anticracking analysis are proposed. Differential element analysis method is adopted to derive the solving formula of the critical cracking state, and then the practical analysis method for longitudinal anti-cracking is established. The influence of some factors on the longitudinal anti-cracking ability is studied. Results show that the section dimensions (thickness of bottom, web and top plate) and prestress eccentricity on web plate are the main factors that influence the anti-cracking ability. Moreover, the proposed method is applied into three engineering examples to make longitudinal anti-cracking verification for the girders. According to the verification results, the design improvements for these girders are determined.

Polyvinylchloride Ultrafiltration Membranes Modified with Different SiO2 Particles and Their Antifouling Mechanism for Oil Extraction Wastewater

Zhao, Fang-Bo,Yu, Zhen-Jiang,Park, Hee-Deung,Liu, Xu-Yang,Song, Xiang-Ru,Li, Zhan-Shuang American Society of Civil Engineers 2015 Journal of environmental engineering Vol.141 No.8