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DNA methylation and mRNA expression of COL6A3 in antler mesenchyme of female and male reindeer
Jian‑Cheng Zhai,Ruo‑Bing Han,Sheng‑Nan Wang,Qiang‑Hui Wang,Yan‑Ling Xia,Wei‑Shi Liu,Ya‑Jie Yin,He‑Ping Li 한국유전학회 2019 Genes & Genomics Vol.41 No.9
Backgroud Reindeer is the only deer species that both male and female produce antlers, which provides a particularly interesting case in studying the differences between antlers of the two sexes. Alpha 3(VI) Collagen Gene (COL6A3), forms a microfibrillar network associated with the structural integrity and biomechanical properties, has been found to be one of the differentially expressed genes in antler mesenchyme of female and male reindeer. Objective and Methods The promoter sequence of reindeer COL6A3 gene was obtained using the cloning technology and analyzed by the bioinformatics methods. Bisulfite sequencing PCR (BSP) was used to detect the methylation status of the COL6A3 promoter in reindeer antler mesenchyme. Real-time quantitative PCR was used to detect COL6A3 expression in the antler mesenchyme of female and male reindeer. Results Sequence analysis revealed that the reindeer COL6A3 partial promoter sequence was 983 bp including the possible promoter region at + 105 bp to + 155 bp. Homology and phylogenetic analysis indicated that the COL6A3 promoter of reindeer had the closest genetic distance with Bos taurus, Capra hircus and Ovis aries. BSP results indicated that the methylation level of COL6A3 promoter in the female reindeer antler mesenchyme was significantly higher than in the male. Correlating with increased methylation status, we also found that COL6A3 mRNA expression in female reindeer antler mesenchyme was significantly lower than in the male. Conclusion The higher methylation level of the COL6A3 gene in female reindeer antler mesenchyme coincides with decreased COL6A3 mRNA expression, thereby affecting the transposon silencing mechanism and possibly contributing to apparent differences of antlers in female and male reindeer.
Jian‑bo Xie,Bei‑bei Liu,Xiang‑yu Wu,Jian‑xun Fu 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.5
This work aims to comprehensively summarize the types and distribution of the isolated Bi particles and MnS inclusions inthe Bi-bearing steels, to study the precipitated behavior of sulfide, and to study the effect of Bi particles on grain sizes andthe volume thermal expansion coefficients of the steels. Results show that the average percentage of the isolated Bi particles,semi-encapsulation, complete encapsulation, back-bridge, tiny encapsulation, cavity bridge Bi–MnS inclusions in the Bibearingsteels was 16.89%, 20.49%, 3.44%, 16.89%, 31.63%, 10.66%, respectively. The average equivalent diameters of thegrains in the 0 ppm, 130 ppm, 240 ppm, 760 ppm, 1200 ppm and 2300 ppm Bi content steels were 20.88, 20.17, 19.54, 18.13,13.98 and 13.25 μm, respectively. The precipitated equilibrium mass concentration product of the MnS (w[Mn]·w[S]) wascalculated to be 1.07, and the solid fraction fS value was 0.61. At 950 °C, the volume thermal expansion coefficient change(ΔVE) in 760 ppm Bi content steel was minimum 4 × 10–10%, while at 461.6 °C the ΔVE was maximum, 0.09%, which meansthat Bi affected the volume thermal expansion of the steel strongest in this temperature range.
Liu Jian(Jian Liu),Fei Jingying(Jingying Fei),Lv Mingkang(Mingkang Lv) 아시아사회과학학회 2022 International Science Research Vol.2 No.3
In recent years, a large number of excellent foreign short video bloggers have been active on major social media platforms, comparing the differences between Chinese and Western cultures, introducing Chinese traditional culture and customs to followers, and becoming a new force in telling Chinese stories. This paper takes the short video blogger “Chris” and his short videos as the research object, and analyze how “foreign influencer” tells Chinese stories in the new media environment, which brings new inspiration for telling Chinese stories and spreading Chinese voices well in the new media environment.
Liu, Jian,Gao, Yun-Hua,Li, Ding-Dong,Gao, Yan-Chun,Hou, Ling-Mi,Xie, Ting Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19
Background: To compare the value of contrast-enhanced ultrasound (CEUS) qualitative and quantitative analysis in the identification of breast tumor lumps. Materials and Methods: Qualitative and quantitative indicators of CEUS for 73 cases of breast tumor lumps were retrospectively analyzed by univariate and multivariate approaches. Logistic regression was applied and ROC curves were drawn for evaluation and comparison. Results: The CEUS qualitative indicator-generated regression equation contained three indicators, namely enhanced homogeneity, diameter line expansion and peak intensity grading, which demonstrated prediction accuracy for benign and malignant breast tumor lumps of 91.8%; the quantitative indicator-generated regression equation only contained one indicator, namely the relative peak intensity, and its prediction accuracy was 61.5%. The corresponding areas under the ROC curve for qualitative and quantitative analyses were 91.3% and 75.7%, respectively, which exhibited a statistically significant difference by the Z test (P<0.05). Conclusions: The ability of CEUS qualitative analysis to identify breast tumor lumps is better than with quantitative analysis.
A UPS BASED ON A NEW SPWM GENERATOR
Liu Shulin,Liu Jian 전력전자학회 1998 ICPE(ISPE)논문집 Vol.- No.-
A new SPWM control method for Uninterrupted Power System(UPS) is presented A triangle waveform is used as the reference signal The desired SPWM control signal can be obtained more easily with a group of comparators The output AC voltage can be regulated by controlling the lower reference and the upper reference of the comparators according to the feedback voltage Basic principle, an actual circuit and the experimental results on a 500W UPS for computer system is discussed as an example.<br/>
IMPROVEMENTS OF SWITCHED-CAPACITOR NETWORKS TO THE PERFORMANCE OF SWITCHING DC-DC CONVERTERS
Liu Jian,Chen Zhiming,Yan Baiping 전력전자학회 1998 ICPE(ISPE)논문집 Vol.- No.-
Three switched-capacitor(SC) networks are presented including series-parallel capacitor set, reversed-switched-capacitor network and push-pull switched-capacitor network., the performances of which are discussed. Combining the SC networks with traditional DC-DC converters, we form several new topologies. Experiment and analyzed results show that the behavior of a DC-DC converter with large voltage ratio can be improved A wider voltage conversion range is also obtained.<br/>
A SWITCHED-CAPACITOR CONVERTERS WITH HIGH PERFORMANCE
Jian Liu,Zhiming Chen,Yanru Zhong 전력전자학회 1995 ICPE(ISPE)논문집 Vol.1995 No.10
Voltage-tracking-control regulation method is employed to improve the properties of switched -capacitor converters The approach to increase the conversion efficiency is discussed An isolating method without employing any transformer is presented AC voltage with frequency as high as 4000 Hz has been inverted with low distortion.
Applicability research of round tube CHF mechanistic model in rod bundle channel
Liu, Wei,Peng, Shinian,Shan, Jianqiang,Jiang, Guangming,Liu, Yu,Deng, Jian,Hu, Ying Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.2
In view of the complex geometric structure of the rod bundle channel and the limitation of the current CHF visualization experiment technology, it is very difficult to obtain the rod bundle CHF mechanism directly through the phenomenon of the rod bundle CHF visualization experiment. In order to obtain the applicable CHF mechanism assumption for rod bundle channel, firstly, five most representative DNB type round tube CHF mechanistic models are obtained with evaluation and screening. Then these original round tube CHF mechanistic models based on inlet conditions are converted to local conditions and coupled with subchannel analysis code ATHAS. Based on 5 × 5 full-length rod bundle CHF experimental data independently developed by Nuclear Power Institute of China (NPIC), the applicability research of each model for CHF prediction performance in rod bundle channel is carried out, and the commonness and difference of each model are comparatively studied. The CHF mechanism assumption of superheated liquid layer depletion that is most likely to be applicable for the rod bundle channel is selected and two directions that need to be improved are given. This study provides a reference for the development of CHF mechanistic model in rod bundle channel.
A New Triterpenoid from Brucea javanica
Jian Hua Liu,Jiang Jiang Qin,Hui Zi Jin,Xiao Jia Hu,Ming Chen,Yun Heng Shen,Shi Kai Yan,Wei Dong Zhang 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.5
A new triterpenoid, bruceajavanin C (1), together with bruceosides A and B (2 and 3), bruceines D and E (4 and 5), yadanziosides A and G (6 and 7), (20R)-O-(3)-α-L-arabinopyranosylpregn- 5-ene-3β,20-diol (8), and α-D-glucopyranoside, (3β, 20R)-3-hydroxypregn-5-en-20-yl (9) were isolated from the aerial parts of Brucea javanica. The structure of 1 was elucidated on the basis of 2D-NMR spectroscopic analysis. In addition, compounds 1, 3, 4, 5, and 6 exhibited mild inhibitory effect on NO production in LPS-stimulated RAW264.7 cells.