[논문]보육실습에서 프로젝트접근법에 대한 예비보육교사의 관심수준 변화 연구

유병민,박수홍,강문숙,박철수,정경훈 교육혁신학술위원회 2011 교육혁신연구 Vol.21 No.1

본 연구는 예비보육교사가 보육실습이라는 과정에서 프로젝트 접근법에 대한 관심은 어떠하며, 관심변화 여부를 사례중심으로 살펴보는 것을 목적으로 하였다. 이를 위하여 예비보육교사를 대상으로 보육실습 사전과 사후검사를 실시하였으며, 자세하고 구체적인 사실과 연구의 질적인 부분을 찾아보고자 인터뷰하였다. 그 결과, 첫째, 프로젝트에 대한 예비보육교사들의 관심은 전반적으로 무관심으로 나타났다. 둘째, 사전․사후검사 결과, 프로젝트 접근법에 대한 관심수준의 변화가 있었던 사례에서는 관심변화의 패턴도 다양하게 나타났으며, 관심변화가 크게 없었던 사례에서는 개인, 정보관심이 높게 나타났다. 이러한 결과는 프로젝트에 대한 예비보육교사의 관심수준은 보육환경, 보육과정, 실습지도 교사, 예비보육교사의 지각력, 프로젝트에 대한 사전지식이나 교육정도에 따라 다양하게 변할 수 있음을 알게 되었다. The purpose of this study is to analyze the case in which we can identify how the SoC of prospective child care teachers about the project approach changes and why these change appears during the child care practice when we assumpt that for prospective child care teachers to implement the project approach is that they participate in change during child care practice. In Case K, the SoC of the prospective child care teacher moves from Stage 0 awareness to Stage 2, Stage 3, Stage 4, and In Case M, the SoC moves from Stage 0 to Stage 1. In Case P, the SoC moves from Stage 1 to Stage 2, Stage 3, and in Case S, the SoC isn't changed. Above this, we identify the SoC of prospective child care teachers. Case K, M, and P changes SoC after child care practice, but Case S doesn't change. the reason why there is a difference is the mindset of prospective child care teachers. Anyone who wants to accomplish specific purposes can deal with diverse changes with flexibility, but others can easily have an effect on the changes. We suggest that instructor in organization for prospective child care teachers’ education need to have a class for prospective child care teachers to put theory into practice, give direct facilitation and intervention in the field to prospective child care teachers, make a learning environment for prospective child care teachers to make good use of teaching materials and instrument, develop guidebook for project approach and give support to prospective child care teachers.

Advanced oxidation and adsorptive bubble separation of dyes using MnO₂-coated Fe₃O₄ nanocomposite

Kang, Yu-Gyeong,Yoon, Hakwon,Lee, Chung-Seop,Kim, Eun-Ju,Chang, Yoon-Seok Elsevier 2019 Water research Vol.151 No.-

<P><B>Abstract</B></P> <P>In this study, MnO<SUB>2</SUB>-coated Fe<SUB>3</SUB>O<SUB>4</SUB> nanocomposite (Fe<SUB>3</SUB>O<SUB>4</SUB>@MnO<SUB>2</SUB>) was utilized to decompose H<SUB>2</SUB>O<SUB>2</SUB> to remove dyes via advanced oxidation processes and adsorptive bubble separation (advanced ABS system). The combination of H<SUB>2</SUB>O<SUB>2</SUB> and Fe<SUB>3</SUB>O<SUB>4</SUB>@MnO<SUB>2</SUB> generated bubbles and formed a stable foam layer in the presence of a surfactant; sodium dodecyl sulfate (SDS) or cetyltrimethylammonium chloride (CTAC), separating dye from the solution. On the basis of radical quenching experiments, electron paramagnetic resonance and X-ray photoelectron spectroscopy analyses, it was confirmed that the MnO<SUB>2</SUB> shell of catalyst was reduced to Mn<SUB>2</SUB>O<SUB>3</SUB> by H<SUB>2</SUB>O<SUB>2</SUB>, generating radicals and oxygen gas for the removal of dyes. In the advanced ABS system, ∙OH and <SUP>1</SUP>O<SUB>2</SUB> were the main radical species and the O<SUB>2</SUB> concentrations of 0.34 and 0.71 mM were increased in the solution and headspace, respectively. The advanced ABS system demonstrated a high removal efficiency of methylene blue (MB) (99.0%) and the removal rate increased with increasing amounts of components (H<SUB>2</SUB>O<SUB>2</SUB>, catalyst and SDS). Also, the advanced ABS system maintained high removal efficiency of MB at a wide pH range of 3–9. In addition to the anionic surfactant of SDS, CTAC was applied as a cationic surfactant for the advanced ABS of anionic dyes. Lastly, the scale-up system was applied to remediate dye-contaminated river water and industrial wastewater for possible practical applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Organic dyes were removed via adsorptive bubble separation with advanced oxidation. </LI> <LI> The MnO<SUB>2</SUB> of catalyst was reduced to Mn<SUB>2</SUB>O<SUB>3</SUB> by H<SUB>2</SUB>O<SUB>2</SUB>, generating radicals and oxygen. </LI> <LI> The separation was efficient toward the dyes of opposite charge to surfactant. </LI> <LI> The scale-up system was applied to dye-contaminated river water and wastewater. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Activation of persulfate by a novel Fe(II)-immobilized chitosan/alginate composite for bisphenol A degradation

Kang, Yu-Gyeong,Vu, Huong Chi,Le, Thao Thanh,Chang, Yoon-Seok Elsevier 2018 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.353 No.-

<P><B>Abstract</B></P> <P>In this study, a novel Fe(II) immobilized chitosan/alginate composite (Fe-Chitoal) was prepared via a simple crosslinking reaction to activate persulfate (PS) to degrade bisphenol A (BPA). The newly synthesized Fe-Chitoal was characterized by scanning electron microscopy, energy dispersive spectroscopy, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy, which indicated that Fe was successfully imprinted into the composite. The PS/Fe-Chitoal system demonstrated a much higher removal efficiency of BPA (99.5%) than the conventional PS/Fe(II) system (69.2%) with a negligible amount of Fe ions released into the solution. The effects of different experimental conditions, including the concentration of PS, concentration of Fe-Chitoal, and pH, on the removal of BPA were also investigated. The removal rate increased with increasing amounts of PS and Fe-Chitoal, and a high removal efficiency was maintained at a pH of 2–10. The PS/Fe-Chitoal system was rarely affected by high concentrations of anions (Cl<SUP>−</SUP>, SO<SUB>4</SUB> <SUP>2−</SUP>, NO<SUB>3</SUB> <SUP>−</SUP>, and HCO<SUB>3</SUB> <SUP>−</SUP>), and the composite catalyst could be reused in a synthetic sewage solution. To simply separate the material, a Fe-Chitoal mesh bag was fabricated, which showed a consistent catalytic reactivity toward PS and BPA removal suggesting practical field applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fe(II)-chitosan/alginate composite was fabricated to activate persulfate. </LI> <LI> The material showed a higher removal of bisphenol A than Fe(II) with persulfate. </LI> <LI> The released Fe ions was readsorbed to the material after reaction with persulfate. </LI> <LI> The system maintained efficiency at wide pH and in the presence of various anions. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Allergenicity Change of Soybean Proteins by Thermal Treatment Methods

Hui-Gyeong Seol(설희경),Yu-Jin Ko(고유진),Eun-Jung Kim(김은정),Gyeong-Lan Lee(이경란),Do-Gyeong Kim(김도경),Jeong-Ok Lee(이정옥),Kang-Mo Ahn(안강모),Chung-Ho Ryu(류충호) 한국생명과학회 2012 생명과학회지 Vol.22 No.4

콩은 우리나라에서 과민성 알레르기를 일으키는 대표적인 식품 중의 하나로, 식품으로 섭취 시에 가열 및 발효가공을 통한 형태로 섭취한다. 이에 본 연구에서는 콩에 알레르기 반응을 일으키는 7명의 환아 혈청과 1명의 정상인 혈청을 이용하여 열처리 방법에 따른 품종별 콩(대풍, 대원, 태광)의 알레르기성에 미치는 영향을 조사하였다. 단백질을 추출하여 SDS-PAGE, immunoblotting 및 ELISA 방법을 통하여 반응성을 조사한 결과, SDS-PAGE상에서 열처리하지 않은 세가지 품종의 경우 9-76 kDa 위치에서 다양한 단백질 밴드를 보였는데 특히 9, 21, 34,52, 72 그리고 76 kDa의 단백질들은 각각 LTP, Kunits trypsin inhibitor, Gly m Bd 30K, β-conglycinin의 β-subunit, α-subunit와 α’-subunit로 주요한 콩 알레르겐으로 알려져 있다. 반면에 볶은 콩, 발효한 콩에서는 35kDa 이하로 완전히 분해되어 열처리 방법을 통해 단백의 항원성이 줄어드는 것을 확인할 수 있었다. IgE immunoblotting 을 통한 세 가지 품종의 볶은 콩과 콩 알레르기 환아 혈청과의 반응에서는 공통적으로 38-40kDa과 10-15 kDa에서 단백질 밴드를 보였으나 발효한 콩에서는 대부분 반응성이 약하거나 나타나지 않았다. ELISA 결과, immunoblotting 분석과 동일하게 대부분의 환아 혈청과 반응시에 볶은 콩과 발효한 콩에서 비교적 낮은 수치를 보였다. 결론적으로 콩에 존재하는 알레르겐 단백질은 열처리와 발효 미생물이 분비하는 단백질 분해효소에 의해 대부분의 환아에서 콩 단백질과의 반응성이 약화되는 것으로 사료된다. Soybean is one of the most common food materials causing food hypersensitivity reactions in Korea. In this study, we have investigated the effect of roasting and fermentation on the allergenicity of soybean. Three kinds of soybean (Daepung, Daewon, and Taegwang) were prepared as raw, roasted, and fermented by Bacillus subtilis GSK 3580, and then their proteins were extracted. The proteins were separated using SDS-PAGE, and the detection of IgE specific to soybean proteins was performed by immunoblotting using 7 sera of soybean allergy patients and non-allergic control individuals. Serum specific IgE to soybean was measured by ELISA. The SDS-PAGE of raw soybean proteins showed various-sized bands ranging from 9 to 76 kDa, which are known as major allergens. In particular, 9, 21, 34, 52, 72, and 76 kDa proteins are known as LTP, Kunits trypsin inhibitor, Gly m Bd 30K, β-subunit, α-subunit, and α’-subunit of β-conglycinin, respectively; these are major allergens in soybean. In contrast, only peptides of less than 35 kDa were found in roasted and fermented soybeans. IgE immunoblot analysis of three roasted species of soybeans commonly detected at 38-40 kDa and 10-15 kDa. The protein bands in fermented soybean showed very weak signals or were not detected. In addition, the reactivity of most patients’ sera to soybean was decreased after roasting and fermentation. With these results, it may be concluded that the allergenicity of soybeans is reduced by the roasting and fermentation processes. It is supposed that allergenic proteins in soybean were degraded by heat treatment methods and proteolytic enzymes were secreted from fermenting microorganisms.

LC-MS/MS를 이용한 우유 중 농약 성분 동시 분석법 개발 및 유효성 검증

한유경(Yu Gyeong Han),최영내(Young Nae Choi),강경원(Gyeong Won Kang),김정년(Jung Nyen Kim),김정복(Jung Bok Kim) 한국식품영양과학회 2020 한국식품영양과학회지 Vol.49 No.8

해마다 연간 우유 소비량이 증가하는 우리나라의 경우, 유해물질 관리와 안전은 더욱 중요하다. 한국작물보호협회에서 자료에 의하면 2014~2018년 농약의 약제별 생산 및 출하 현황 중 살충제가 가장 많은 것으로 보고되어 있다. 카바메이트계 농약은 생물학적 효과가 좋아 살충제, 살균제 및 구충제 등 다양하게 사용되고 있다. 카바메이트계 농약은 일부는 높은 급성 독성을 가지고 있기 때문에 식품에 사용되는 것을 조심해야 하고, 일부 물질의 경우 발암물질을 발생시키고 돌연변이를 야기할 수 있다고 보고되었다. 본 연구에서는 다양한 카바메이트계 농약 중 국내에서 많이 사용되고 있는 살충제의 3가지 농약을 선정하여 우유 원료 중 bendiocarb, carbaryl과 carbofuran 등에 대한 효율적이고 효과적인 분석법을 개발하였고 유효성 검증을 실시하였다. 시료를 무수황산나트륨과 디클로로메탄을 넣고 진탕추출 후 탈수한 뒤 농축하였다. 이를 헥산과 헥산포화아세토니트릴을 이용하여 지방을 제거하고 C18 카트리지를 이용하여 정제 후 LCMS/MS에 주입하여 분석한 결과, bendiocarb, carbaryl 및 carbofuran의 표준원액을 blank를 이용하여 5~100 ng/mL 범위에서 결정계수(r2) 0.999 이상의 직선성을 확인하였다. Bendiocarb의 일내 정밀성은 4.0% 이하, 정확성은 96.2~103.9%, carbaryl의 정밀성은 4.3% 이하, 정확성은 96.9~104.5%, carbofuran의 정밀성은 5.3% 이하, 정확성은 90.9~109.1%로 확인되었고, bendiocarb의 일간 정밀성은 6.5% 이하, 정확성은 90.1~103.7%, carbaryl의 정밀성은 10.5% 이하, 정확성은 95.3~103.3%, carbofuran의 정밀성은 14.8% 이하, 정확성은 94.2~108.5%로 확인되었다. Bendiocarb, carbaryl 및 carbofuran의 평균 회수율은 103.6~110.7%였다. 이때 상대표준편차는 모두 4.9% 이하로 확인되었다. 3가지 성분 각각의 검출한계는 0.8~1.5 ng/mL, 정량한계는 3.0~6.0 ng/mL로 확인되었다. 측정불확도를 산출한 결과 bendiocarb는 14.17±0.82 ㎎/㎏, carbaryl은 14.34±0.91 ㎎/㎏, carbofuran은 14.28±0.79 ㎎/㎏(신뢰수준 95%, K=2)으로 나타났다. 따라서 본 연구에서는 우유 중 bendiocarb, carbaryl 및 carbofuran의 정성 및 정량분석을 위해 유효성이 검증된 동시 분석법을 확립함으로써 향후 우유 중 잔류물질 기준규격 설정 및 관리에 참고 자료가 될 수 있다고 판단된다. Carbamate pesticides are compounds obtained by the reaction of carbamic acid and amine. These pesticides are often used as insecticides and herbicides. Especially, bendiocarb, carbaryl and carbofuran are popular carbamate pesticides LC-MS/MS using liquid-liquid extraction (LLE) and C18 cartridges was used to identify and quantity the levels of bendiocarb, carbaryl and carbofuran in raw material milk with high sensitivity and specificity. A calibration curve was obtained from 5 to 100 ng/mL with a satisfactory correlation coefficient of 0.999. The limit of detection (LOD) and limit of quantitation (LOQ) for bendiocarb, carbaryl and carbofuran in the matrix were 0.8 ng/mL to 1.5 ng/mL and 3.0 ng/mL to 6.0 ng/mL, respectively. The recovery of the three pesticides from spiked samples at 15 ng/mL ranged from 103.6 to 110.7% with a relative standard deviation (RSD) between 4.6 to 4.9%. The measurement of uncertainty of the LC-MS/MS method developed for the three pesticides was based on the analytical process and quantification. Thus, the current study has established a fase and easy method for the analysis of bendiocarb, carbaryl and carbofuran in raw material milk by LC-MS/MS.

Development of Cleaved Amplified Polymorphic Sequence Markers for Classifying Ginger (Zingiber officinale) Cultivars Using Reference Sequencing

Ji-Nam Kang(Ji-Nam Kang),Gyeong-Hui Lee(Gyeong-Hui Lee),Jin Yu(Jin Yu),Mi-Hwa Choi(Mi-Hwa Choi),Simyung Lee(Simyung Lee) 한국육종학회 2023 Plant Breeding and Biotechnology Vol.11 No.2

Ginger (Zingiber officinale) is grown worldwide in subtropical and tropical regions and primarily used as a spice and medicinal plant. Despite the economic importance of ginger, research on its molecular aspects is limited. Moreover, although ginger is mainly cultivated through vegetative propagation owing to poor flowering and infertility, few molecular markers have been identified to distinguish cultivars. In this research, we developed five Cleaved Amplified Polymorphic Sequence (CAPS) markers that can distinguish between the “Bongdong” ginger (Bg) cultivar, indigenous to South Korea, and the Chinese imported ginger (Cg) cultivar through reference sequencing based on the recently reported complete genome information of ginger. Furthermore, the integrated application of the five CAPS markers allow us to distinguish between Bg, Cg, and Indonesian ginger. Among them, the ClaI-based CAPS marker was identified as specific to Bg cultivars. Therefore, TaqMan real-time PCR based on ClaI-based CAPS can be widely used to distinguish between Bg and Cg cultivars. This study is the first to report the development of genome-based single-nucleotide polymorphism markers in ginger and therefore provides important information for the breeding and conservation of ginger.

제왕절개술 후 발생한 산후 난소 정맥 혈전증

이유경 ( Lee Yu Gyeong ),윤대석 ( Yun Dae Seog ),유재두 ( Yu Jae Du ),강창구 ( Kang Chang Gu ),나재형 ( Na Jae Hyeong ),배열 ( Bae Yeol ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.8

Postpartum ovarian vein thrombosis has been thought to be a rare disease of which clinical finding is not accurate. Most of postpartum ovarian vein thrombosis presents as symptoms consisting of fever, lower abdominal pain, abdominal mass. If the symptom d

Flowing with Jeju heanyeo - Jeju National University High school club Tewak is floating

Hong Ji Hee,Kang Gyeong Ji,Kim Eun Hee,Kim On Yu,Lee Yu Bin,Ko Jun Hyeok,Kang Min Jae,Yang Hyeon Jeong,Yang Yeon Jae,Sim Yu Rim,Kim Min Ji,Geum Na Hyeon,Kim Geum Byeol,Heo Jeong In 제주학회 2016 제주학회 학술발표논문집 Vol.2016 No.2

Protective Effect of Semi-Fermented Tea on Neuroinflamation and nerooxidation

Yu Rim Lee,Gyeong-Ji Kim,Kwon-Jai Lee,Gyo Ha Moon,Khoriunnisa Ratih,Kang-Hyun Chung,Min Hyeong Kim,Jeung Hee An 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

The C allele of synonymous SNP (rs1142636, Asn170Asn) in <i>SYN1</i> is a risk factor for the susceptibility of Korean female schizophrenia

Yu, Gyeong Im,Kim, Su Kang,Park, Hae Jeong,Kim, Jong Woo,Chung, Joo‐,Ho,Shin, Dong Hoon Wiley Subscription Services, Inc., A Wiley Company 2012 Synapse Vol.66 No.11

<P><B>Abstract</B></P><P><B>Objective:</B> The aim of this study was to investigate the association between the exonic single nucleotide polymorphisms (SNPs) of <I>synapsin I</I> (<I>SYN1</I>) (rs1142636, Asn170Asn, Xp11.23) and <I>SYN2</I> (rs2289708, 3′‐untranslated region, 3p25) in schizopherenia. <B>Methods:</B> Two hundred eighty six schizophrenia patients and 304 control subjects were recruited. SNPs with a know heterozygosity and minor allele frequency (MAF) > 0.1 in Asian populations were selected and genotyped by direct sequencing. <B>Results:</B> The allelic frequencies of rs1142636 (<I>SYN1</I>) were associated with schizophrenia (<I>P</I> < 0.05), respectively. The allelic frequency of rs1142636 in all subjects was associated with schizophrenia [<I>P</I> = 0.000059, OR = 2.17 (95% CI = 1.47–3.18)]. The C allele frequency of rs1142636 was higher in schizophrenia (20.8%) than that in controls (10.8%). In the analysis of gender, the allelic frequency of rs1142636 was also strongly associated with female schizophrenia [<I>P</I> = 0.0001, OR = 2.65 (95% CI = 1.61–4.36)], but not with male schizophrenia. The C allele frequency of rs1142636 was higher in female schizophrenia (22.2%) than that in female controls (9.7%). The rs2289708 SNP (<I>SYN2</I>) did not show any association between schizophrenia and controls. <B>Conclusions:</B> These results suggest that the C allele of a synonymous SNP (rs1142636, Asn170Asn, Xp11.23) in <I>SYN1</I> may be a risk factor for the susceptibility of Koreran female schizophrenia. Synapse 66:979–983, 2012. © 2012 Wiley Periodicals, Inc.</P>