RhoGDI2 induced malignant phenotypes of pancreatic cancer cells via regulating Snail expression

Yi Bin,Hu You,Zhu Dongming,Yao Jun,Zhou Jian,Zhang Yi,He Zhilong,Zhang Lifeng,Zhang Zixiang,Yang Jian,Tang Yuchen,Huang Yujie,Li Dechun,Liu Qiuhua 한국유전학회 2022 Genes & Genomics Vol.44 No.5

Background: Rho GDP dissociation inhibitor 2 (RhoGDI2) has been shown to contribute to the aggressive phenotypes of human cancers, such as tumor metastasis and chemoresistance. Objective: This study aimed to assess the effects of RhoGDI2 on tumor progression and chemoresistance in pancreatic cancer cells. Methods: The expression of RhoGDI2 in pancreatic cancer cells was detected by Western blot analysis. Gain-of-function and loss-of-function approaches were done to examine the malignant phenotypes of the RhoGDI2-expressing or RhoGDI2-depleting cells. The correlation between RhoGDI2 and Snail was also analyzed. Results: Differential expression of RhoGDI2 protein in pancreatic cancer cell lines was identified. Gain-of-function and loss-of-function experiments showed that RhoGDI2 induced the malignant phenotypes of pancreatic cancer cells, including proliferation, migration, invasion, and gemcitabine (GEM) chemoresistance. The upregulation of RhoGDI2 stimulated the expression of Snail, resulting in the altered expression of epithelial marker E-cadherin and mesenchymal marker Vimentin, which were characteristics of the tumorigenic activity of epithelial-mesenchymal transition. The expression of RhoGDI2 and Snail was upregulated in clinical tumor samples, and higher expression of RhoGDI2 or Snail was significantly associated with poor patient survival in pancreatic ductal adenocarcinoma (PDAC). Conclusion: The findings indicated that RhoGDI2 promoted GEM resistance and tumor progression in pancreatic cancer and that RhoGDI2 might be a potential therapeutic target in patients with PDAC.

Real-Time Apartment Building Detection and Tracking with AdaBoost Procedure and Motion-Adjusted Tracker

Yi Hu,Dae Sik Jang,Chang Woo Lee,Jeong Ho Park,Seong Ik Cho 한국전자통신연구원 2008 ETRI Journal Vol.30 No.2

In this letter, we propose a novel approach to detecting and tracking apartment buildings for the development of a video-based navigation system that provides augmented reality representation of guidance information on live video sequences. For this, we propose a building detector and tracker. The detector is based on the AdaBoost classifier followed by hierarchical clustering. The classifier uses modified Haar-like features as the primitives. The tracker is a motion-adjusted tracker based on pyramid implementation of the Lukas-Kanade tracker, which periodically confirms and consistently adjusts the tracking region. Experiments show that the proposed approach yields robust and reliable results and is far superior to conventional approaches.

Resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates

Yi-Jing Wang,Pan Zhao,Bing-Dong Sui,Nu Liu,Cheng-Hu Hu,Ji Chen,Chen-Xi Zheng,An-Qi Liu,Kun Xuan,Ya-Ping Pan,Yan Jin 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Mesenchymal stem cell (MSC)-based regeneration, specifically cell aggregate or cell sheet engineering, is a promising approach for tissue reconstruction. Considering the advantages of ease of harvest and lack of immune rejection, the application of autologous MSCs (i.e., patients’ own MSCs) in regenerative medicine has developed considerable interest. However, the impaired cell viability and regenerative potential following MSCs impacted by disease remain a major challenge. Resveratrol (RSV) exhibits reliable and extensive rejuvenative activities that have received increasing clinical attention. Here, we uncovered that resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates. Periodontal ligament MSCs (PDLSCs) from normal control subjects (N-PDLSCs) and periodontitis patients (P-PDLSCs) were investigated. Compared to N-PDLSCs, P-PDLSCs were less capable of forming cell aggregates, and P-PDLSC aggregates showed impaired osteogenesis and regeneration. These functional declines could be mimicked in N-PDLSCs by tumor necrosis factor alpha (TNF-α) treatment. Notably, a TNF-α-induced functional decline in N-PDLSC aggregates was rescued by RSV application. More importantly, in both N-PDLSCs and P-PDLSCs, RSV promoted cell aggregate formation and improved their osteogenic potential. Furthermore, as proven ectopically in vivo, the tissue regenerative capability of P-PDLSC aggregates was also enhanced after RSV treatment during aggregate formation in vitro. Finally, in a rat in situ regeneration model, we successfully applied both N-PDLSC aggregates and P-PDLSC aggregates to repair periodontal defects upon long-term functional improvements by RSV preconditioning. Together, our data unravel a novel methodology for using pharmacology (i.e., RSV)-based cell aggregate engineering to improve the functionality and facilitate the regeneration of MSCs from both healthy and inflammatory microenvironments, shedding light on improving the application of autologous MSC-mediated regenerative medicine.

Conflicts between Traditional Animation and Modern Animation in China

Hu Yi Hong(胡依紅) 한국만화애니메이션학회 2004 한국만화애니메이션학회 학술대회자료집 Vol.2004 No.11

ON THE COMPLEX VARIABILITY OF THE SUPERORBITAL MODULATION PERIOD OF LMC X-4

HU, CHIN-PING,LIN, CHING-PING,CHOU, YI,YANG, TING-CHANG,SU, YI-HAO,HSIEH, HUNG-EN,CHUANG, PO-SHENG,LIAO, NAI-HUI The Korean Astronomical Society 2015 天文學論叢 Vol.30 No.2

LMC X-4 is an eclipsing high-mass X-ray binary exhibiting a superorbital modulation with a period of ~ 30:5 days. We present a detailed study of the variations of the superorbital modulation period with a time baseline of ~ 18 years. The period determined in the light curve collected by the Monitor of All-sky X-ray Image (MAXI) significantly deviates from that observed by the All Sky Monitor (ASM) onboard the Rossi X-ray Timing Explorer (RXTE). Using the data collected by RXTE/ASM, MAXI, and the Burst Alert Telescope (BAT) onboard Swift, we found a significant period derivative, $\dot{P}=(2.08{\pm}0.12){\times}10^{-5}$. Furthermore, the O{C residual shows complex short-term variations indicating that the superorbital modulation of LMC X-4 exhibits complicated unstable behaviors. In addition, we used archive data collected by the Proportional Counter Array (PCA) on RXTE to estimate the orbital and spin parameters. The detected pulse frequencies obtained in small time segments were fitted with a circular orbital Doppler shift model. In addition to orbital parameters and spin frequency for each observation, we found a spin frequency derivative of $\dot{v}=(6.482{\pm}0.011){\times}10^{-13}Hz{\cdot}s^{-1}$. More precise orbital and spin parameters will be evaluated by the pulse arrival time delay technique in the future.

Knockdown of LncRNA H19 Relieves LPS-Induced Damage by Modulating miR-130a in Osteoarthritis

Yi Hu,Sukai Li,Yonggen Zou 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.4

Purpose: Osteoarthritis (OA) is a commonly occurring illness without a definitive cure, at present. Long non-coding RNAs (lncRNAs)have been widely confirmed to be involved in the modulation of OA progression. This study aimed to investigate the roleand mechanism of lncRNA H19 in OA. Materials and Methods: Abundances of H19 and microRNA-130a (miR-130a) in lipopolysaccharide (LPS)-treated C28/I2 cellswere measured by reverse-transcription quantitative PCR (RT-qPCR). CCK-8 and flow cytometry analyses were carried out to assesscell viability and apoptosis. Starbase online software was used to predict the putative binding sites between H19 and miR-130a. Luciferase reporter, RNA pull down, and RT-qPCR were performed to analyze the true interaction between H19 and miR-130a. Results: A notably dose-dependent elevation of H19 levels was observed in LPS-treated C28/I2 cells. Knockdown of H19 amelioratedthe injury of LPS-induced C28/I2 cells, reflected by induced viability, decreased apoptosis, and reduced inflammatory factorsecretions. Moreover, H19 negatively regulated the expression of miR-130a via acting as a molecular sponge for miR-130a. Thestimulatory effects of H19 on cell damage were abolished following the restoration of miR-130a. Conclusion: LncRNA H19 aggravated the injury of LPS-induced C28/I2 cells by sponging miR-130a, hinting a novel regulatorymechanism and a potential therapeutic target for OA.

Vision based place recognition using Bayesian inference with feedback of image retrieval

( Hu Yi ),( Chang Woo Lee ) 한국정보처리학회 2006 한국정보처리학회 학술대회논문집 Vol.13 No.2

In this paper we present a vision based place recognition method which uses Bayesian method with feed back of image retrieval. Both Bayesian method and image retrieval method are based on interest features that are invariant to many image transformations. The interest features are detected using Harris-Laplacian detector and then descriptors are generated from the image patches centered at the features’ position in the same manner of SIFT. The Bayesian method contains two stages: learning and recognition. The image retrieval result is fed back to the Bayesian recognition to achieve robust and confidence. The experimental results show the effectiveness of our method.

Mechanism and Experimental Investigation of Ultra High Pressure Water Jet on Rubber Cutting

Yi Hu,Yong Kang,Xiao-Chuan Wang,Xiao-Hong Li,Xinping LONG,Guang-Yao Zhai,Man Huang 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.

The recycle and reuse of rubber materials has become a global environment problem with the fast increasing amount of waste rubber. Compared to traditional recycle methods, ultra high pressure water jet, as a fast developing tool of cool cutting, can be used inrecycling rubber materials without damaging the internal organization structure of materials. This paper investigates ultra highpressure jet cutting rubber in mechanism and experiment. Different from metallic materials and brittle materials, rubber materialsare cut by the force of shear and tensile under the impinging of ultra high pressure water jet and the erosion of high speed waterflow with rubber powder. The overall structure of kerf is Y shape. The upper section of Y shape is caused by shear, the middle sectionis resulted from both shear and tensile, and the bottom section is a result of the erosion and the tensile. The results of experimentshow: (1) a significant linear correlation between cutting depth and pump pressure;(2) 2.0 mm/s is the optimal transverse velocityunder the current experiment condition;(3) the cutting depth has a decreasing tendency with the stand-off distance increased. It canbe concluded from orthogonal analysis that pump pressure has a signification effect on cutting depth, nozzle transverse velocity isnot remarkable, and the effect of stand-off distance is not obvious.

Computer vision-based displacement measurement with m-sequence target

Yi-Ding Hu,Qi Xia,Rong-rong Hou,Yong Xia,Jian-yi Yan 국제구조공학회 2021 Smart Structures and Systems, An International Jou Vol.27 No.3

The development of image sensors enables the application of vision-based techniques to the non-contact displacement measurement of large-scale structures. The features of the physical targets are critical to the accuracy, stability and anti-interference of the displacement measurement results. In this study, a novel m-sequence target and the associated circular correlation processing technique are developed for real-time displacement measurement. The properties of the m-sequence as a pseudo-random sequence are introduced. The vision-based displacement calculation method is then derived from the correlation property of the m-sequence. The algorithms and measurement systems are integrated in the LabVIEW environment. To verify the anti-interference performance of the developed system, static and dynamic experimental tests are carried out with various forms of interference, such as partial occlusion, uneven illumination, out of focus and smoke effect. Experimental results indicate that the developed system cannot only accurately measure structural displacement, but also has outstanding antiinterference performance, even if 30% of the target is masked.

Chirality study at the liquid/HOPG interface based on the anthraquinone derivatives

Yi HU,Li XU,Kai MIAO,Wenli DENG 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8