McCoy 세포에서 Chlamydia trachomatis의 병원성 인자에 관한 연구

강정숙,최지영,차영주,김영주,김덕례,여명구,박열 조선대학교 생명과학연구소 1998 생명과학 연구 Vol.6 No.-

본 연구는 병원성 세균인 Chlamydia trachomatis(C. trachomatis)가 숙주세포에침습시 C. trachomatis의 병원성 인자와 C. trachomatis와 상호작용하는 숙주 세포막의 인자를 규명함으로써 숙주세포에 대한 감염율을 향상시켜 보다 향상된 진단 방법을 개발하기 위하여 수행하였다. C. trachomatis로 감염된 세포에서 기본소체의 막 단백질을 분리한 결과 각 혈청형의 39 kDa과 42 kDa에서 major outer membranes(MOMPs)이 관찰되었으며, 혈청형 E와 G의 MOMPs는 42.5 kDa으로 동일하였지만 혈청형 F, H, I, 그리고 K의 MOMPs는 각각 다르게 관찰되었다. C. trachomatis가 숙주세포로 침습시 관련된 병원성 인자에 대하여 조사하고자 단층세포로 배양된 배양용기에 C. trachomatis를 24, 48 그리고 73시간대별로 접종시켜 관찰한 결과 72시간의 MOMPs의 양이 가장 많이 증가하였으며 이 결과로 MOMPS이 C. trachomatis 병원성에 중요한 작용을 함을 확인할 수 있었다. C. trachomatis가 침습시 숙주세포의 막 단백질 변화양상을 관찰하기 위해 정상세포와 C. trachomatis로 1, 2, 3 그리고 4시간대별로 접종 시간을 달리한 숙주세포의 막 단백질을 분리한 결과 42 kDa의 단백질 양이 Chlamydia에 감염된 세포 내에서 증가됨을 관찰할 수 있었다. 이로 42 kDa의 막 단백질이 침습시 숙주세포 표면에 대한 수용체로서 작용함을 알 수 있었다. The present study was performed to analyze the pathogenic factors involved in the invasion process of Chlamydia trachomatis(C. trachomatis) into McCoy cells. In this study, elementary bodies(EBs) of C. trachomatis serotypes(E, F, G, H, I, K and LGV) could be isolated by the method of Percoll density gradient centrifugation and the pathogens of serotypes were also compared from whole-cell lysates by using sodium dodecyl sulfate-polyacrylamide gel elelectrophoresis. All chlamydial serotypes showed a single predominant protein ranging from 39 to 42 kDa. The major outer membrane proteins(MOMPs) of the E and G serotypes had an identical molecular weight of 42.5 kDa. In contrast, the MOMPs of the F, H, I and K serotypes were showed different molecular weights of MOMPs. By immunoblotting with anti-MOMP antibody it was revealed that the quantify of MOMPs was time-dependently increased when McCoy cells were infected with Chlamydia for 24, 48 and 72 hr. These results indicated that MOMPs are closely related to chlamydial infection into the host cell.

Diagnosis of Chlamydial infection by using monoclonal antibody

Yeo, Myeong-Gu,Kim, Ho-Sang,Kim, Young-Ju,Kim, Sung-Jun,Kim, Jong-Se,Choi, Young-Bok,Park, Yeal 조선대학교 생명과학연구소 1997 생명과학 연구 Vol.5 No.-

Hybrid cell line that produced MAbs against Chlamydia trachomatis surface antigens were prepared by the fusion of mouse myeloma cells with BALB/c mouse. Those Mabs were used to examine the antigenic specificity and biological function of several Chlamydia serotypes. Using those MABs, chlamydial inclusions were detected by using chemiluminence and it turns out that the chemiluminents method was very useful to detect the chlamydial inclusions or infection. It was confirmed that this method is easy to detect and to reduce the time consuming compared with examination of chlamydial inclusions order fluorescence microscopy.

SURVEY ON THE AFLATOXINS OF DISTRIBUTED MEDICAL HERBS IN KOREA

Seung young Park,Soo yeul Cho,Hyun Ju Moon,Ji young Noh,Hwa-mi Lee,Eun gui Kang,Jun gu Lee,Eun Ryong Park,Ji young Song,Sung il Kim,Ok Sun Cho,Yeo Won Sohn,Chan soon Kang,Dae hyun Cho 한국환경독성학회 2009 한국독성학회 심포지움 및 학술발표회 Vol.2009 No.11

Air-Soil Partitioning of PCBs in Rural Area

Hyun-Gu Yeo,Minkyu Choi,Man-Young Chun,Young Sunwoo 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.E1

The soil concentrations of polychlorinated biphenyls(PCBs) were measured at 12 sites in Ansung, Kyonggi province, Korea. Correlation coefficient(r) between total PCBs and organic matter content(OM) was significant(r=0.562, p<0.05). It suggests that organic matter may be a key factor of soil absorption of PCBs. The PCB concentrations of law chlorinated congeners with high vapor pressure were relatively abundant in air but high chlorinated congeners with low vapor pressure were mainly dominated by soil. The results indicated the influence of physicochmical properties of PCBs such as vapor pressure, octanal-air partition coefficient(K_OA). The calculated soil/air fugacity quotients suggested that the soil may be a source of heavier molecular PCBs(>penta-CBs) to the atmosphere, where lighter molecular PCBs appear to be affected by a movement from air to soil, especially tetra-CBs. Therefore, PCB homologs with low vapor pressure might have been influenced by revolatilization from soil.

도시 및 교외지역에서 대기 중 PCBs 의 온도의존성을 이용한 발생원 특성

여현구(Hyun Gu Yeo),최민규(Min Kyu Choi),천만영(Man Young Chun),선우영(Young Sunwoo) 大韓環境工學會 2002 대한환경공학회지 Vol.24 No.5

수박 세균성 점무늬 병의 저항성 품종육성을 위한 병원균의 특성 및 분류동정

여순남,빈철구,박영배,손병구,김병수 密陽大學校 生命資源開發硏究所 2003 生命資源開發硏究 Vol.7 No.-

수박 세균성 점무늬 병에 걸린 잎과 과실의 병반에서는 King's medium B에 회백색의 군락을 형성하고 비 형광성이며 그램 양성세균이 분리되었다. 이 세균은 KMB배지에서 가장 잘 생육하였으며 병원성도 가장 오래 유지되었다 단기보존을 위해서는 KMB배지에 생육시켜 25℃의 항온기에 보관해 두는 것이 적합한 것으로 나타났다. 그리고 이 세균은 봉상으로서 한개의 편모를 가져 운동성이 있었고 41℃에서 생육가능하였으며 Oxidase와 2-ketogluconate 형성에 양성반응을 나타내었다. Giucose, Fructose, Xylose등을 탄소원으로서 생육에 이용하였다. Nitrate reduction, 담배에의 과민감반응 tween-80 bydrolysis는 양성반응, Gelatin액화, Arginine hydrolysis, Starch hydrolysis는 음성반응을 나타내었다. 위의 결과에 의하면 Bergey's manual of systematic bacterilogy에 나타난 P. pickettil와 특성이 거의 일치였다. Bacterial spot of Watermelon is caused by Pseudomonas syringae pv. lachrymans. It's affects the leaves, stems, and fruits of cucumber, cantaloupe, squash, and watermelon. And the worst damage resulted from fruit rot. A gram positive bacterial disease, non-fluorescent bacterium was isolated from the disease tissue. Studies were conducted to identify the pathogenic bacterium. The watermelon bacterium was aerobic, rod-shaped, gram positive, and motile by means of single polar flagellum. The bacterium grew at 41℃, and was positive in oxidase, and 2-ketogluconate. The bacterium was able to utilize glucose, fructose, xylose, for growth as a sole source of carbon compound, bur not able to utilize sucrose, mannitol. mannose, cellobiose, and arabinose. The bacterium reduced nitrate, induced a hypersensitive reaction in tobacco, hydrolyzed Tween 80, was not able to hydrolyzed arginine and starch. On the basis of the above test results, the bacterium was phenomenally Similar to Pseudomonas pickettii. This bacterium grow well on King's(KMB) and the KMB tube was good for a short term storage of the bacterium.

Ginsenoside fractions regulate the action of monocytes and their differentiation into dendritic cells

Yeo Jin Lee,Young Min Son,Min Jeong Gu,Ki-Duk Song,Sung-Moo Park,Hyo Jin Song,Jae Sung Kang,Jong Soo Woo,Jee Hyung Jung,Deok-Chun Yang,Seung Hyun Han,Cheol-Heui Yun 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.3

Background: Panax ginseng (i.e., ginseng) root is extensively used in traditional oriental medicine. It is a modern pharmaceutical reagent for preventing various human diseases such as cancer. Ginsenosidesdthe major active components of ginsengdexhibit immunomodulatory effects. However, the mechanism and function underlying such effects are not fully elucidated, especially in human monocytes and dendritic cells (DCs). Methods: We investigated the immunomodulatory effect of ginsenosides from Panax ginseng root on CD14⁺ monocytes purified from human adult peripheral blood mononuclear cells (PBMCs) and on their differentiation into DCs that affect CD4⁺ T cell activity. Results: After treatment with ginsenoside fractions, monocyte levels of tumor necrosis factor (TNF)-a, interleukin (IL)-6, and IL-10 increased through phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and c-Jun N-terminal kinase (JNK), but not p38 mitogen-activated protein kinase (MAPK). After treatment with ginsenoside fractions, TNF-α production and phosphorylation of ERK1/2 and JNK decreased in lipopolysaccharide (LPS)-sensitized monocytes.We confirmed that DCs derived from CD14⁺ monocytes in the presence of ginsenoside fractions (Gin-DCs) contained decreased levels of the costimulatory molecules CD80 and CD86. The expression of these costimulatory molecules decreased in LPS-treated DCs exposed to ginsenoside fractions, compared to their expression in LPS-treated DCs in the absence of ginsenoside fractions. Furthermore, LPS-treated Gin-DCs could not induce proliferation and interferon gamma (IFN-γ) production by CD4⁺ T cells with the coculture of Gin-DCs with CD4⁺ T cells. Conclusion: These results suggest that ginsenoside fractions from the ginseng root suppress cytokine production and maturation of LPS-treated DCs and downregulate CD4⁺ T cells.

세균성 점 무늬병의 기주범위 및 수박품종의 저항성 비교

여순남,빈철구,박영배,강점순,김병수 密陽大學校 生命資源開發硏究所 2003 生命資源開發硏究 Vol.7 No.-

세균성 점무의 병의 증상으로는 잎에서 수침상의 반점이 형성되어 점차 커지면서 암갈색의 잎 반점 혹은 잎 마름의 양상으로 진전되었다. 과실에는 암갈색의 반점이 형성되어 심한 경우 과피에 구멍을 내어 과실 내부부패와 상품성에도 큰 차질을 가져오게 되었다. 기주범위 조사를 위해 십자화과의 무, 배추, 양배추와 박과의 수박, 오이, 호박, 참외와 가지과의 고추, 토마토 그리고 국화과의 상추의 엽육조직에 수박세균성 점무늬 병균을 주사접종한 결과 공시한 모든 작물의 접종부위에서 조직이 붕괴 고사하게 되었다. 16개 시판 F1품종에 대하여 저항성 검정을 실시한 결과 종자 접종하여 파종하였을 때는 발아한 자엽의 70~80%가 이병성을 나타내었으며, 본엽의 앞뒷면에 분무접종 하였을 때는 품종간 발병정도에 다소차이가 있었으나 저항성인 품종은 발견되지 않았다. Angular leaf spot of fruits vegetable is caused by Pseudomonas syringae pv. lachrymans. At first, small circular spots appear on the leaves and soon become large, angular to irregular, water-soaked areas. Infected fruits show small, circular, usually superficial spots. Affected tissues die, turn white, and crack open, and then soft-rot fungi and bacteria enter and rot the whole fruit. The bacteria overwinter on contaminated seed and in infected plant refuse. From there the bacteria are splashed to cotyledon and leaves, which they penetrate through stomata and wounds, and move systematically to other parts of the plant. The bacterium was pathogenic on Cucurbitaceae, Cruciferae, Solanaceae, and Compositae, sixteen watermelon cultivars were tested in the vinyl house for resistance to pseudomonas pickettii. Although some variation in susceptibility was observed among commercial varieties but a high of resistance was not found.

Treatment Outcomes of Three-Dimensional Conformal Radiotherapy for Stage Ⅲ Non-Small Cell Lung Cancer

Yeo, Seung-Gu,Cho, Moon-June,Kim, Sun-Young,Lim, Seung-Pyung,Kim, Ki-Hwan,Kim, Jun-Sang 충남대학교 암공동연구소 2006 암공동연구소 업적집 Vol.5 No.

Purpose: To evaluate the treatment outcomes of the three-dimensional conformal radiotherapy (3D-CRT), in conjunction with induction chemotherapy, for the treatment of stage III non-small cell lung cancer (NSCLC). Materials and Methods: Between November 1998 and March 2003, 22 patients with histologically proven, clinical stage III NSCLC, treated with induction chemotherapy, followed by 3D-CRT, were retrospectively analyzed. There were 21 males (96%) and 1 female (4%), with a median age of 68.5 (range, 42~79). The clinical cancer stages were IIIA and IIIB in 41 and 59%, respectively. The histologies were squamous cell carcinoma, adenocarcinoma and others in 73,18 and 9%, respectively. Twenty patients (91%) received induction chemotherapy before radiation therapy. The majority of the chemotherapy regimen consisted of cisplatin and gemcitabine. Radiation was delivered with conventional anteroposterior/ posteroanterior fields for 36 Gy, and then 3D-CRT was performed. The total radiation dose was 70.2 Gy. The median follow-up period was 17 months (range, 4~59months). Results: The median overall survival was 19 months. The two and four-year overall survival rates were 37.9 and 30.3%, respectively. The median progression-free survival was 21 months. The two and four-year progression-free survival rates were 42.1 and 21%, respectively. The prognostic factors for overall survival by a univariate analysis were age, histology and T stage (p<0.05). Acute radiation toxicities, as evaluated by the RTOG toxicity criteria, included two cases of grade 3 lung toxicity and one case of grade 2 esophagus toxicity. Conclusion: The radiation dose could be increased without a significant increment in the acute toxicities when using 3D-CRT. It also seems to be a safe, well-tolerated and effective treatment modality for stage III NSCLC. (Cancer Res Treat. 2005;37:273-278)

Partial Characterization of the Pathogenic Factors Related to Chlamydia trachomatis Invasion of the McCoy Cell Membrane

Yeo, Myeng-Gu,Kim, Young-Ju,Park, Yeal The Microbiological Society of Korea 2003 The journal of microbiology Vol.41 No.2

The present study was performed to identify pathogenic factors of Chlamydia trachomatis, which invade the host cell membrane. We prepared monoclonal antibody against C. trachomatis and searched for pathogenic factors using this antibody, and subsequently identified the surface components of the elementary body of C. trachomatis, i.e., major outer membrane protein (MOMP), lipopolysaccharide (LPS), and two other surface exposure proteins. These proteins are believed to be important in the pathogenesis of host cell chlamydial infection. Additionally, to identify factors related to the host cell and C. trachomatis, we prepared C. trachomatis infected and non-infected McCoy cell extracts, and reacted these with anti-chlamydial LPS monoclonal antibody. We found that anti-chlamydial LPS monoclonal antibody reacted with a 116 kDa proteinaceous McCoy cell membrane component.