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Removal of the Glycosylation of Prion Protein Provokes Apoptosis in SF126
Chen, Lan,Yang, Yang,Han, Jun,Zhang, Bao-Yun,Zhao, Lin,Nie, Kai,Wang, Xiao-Fan,Li, Feng,Gao, Chen,Dong, Xiao-Ping,Xu, Cai-Min Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5
Although the function of cellular prion protein (PrP$^C$) and the pathogenesis of prion diseases have been widely described, the mechanisms are not fully clarified. In this study, increases of the portion of non-glycosylated prion protein deposited in the hamster brains infected with scrapie strain 263K were described. To elucidate the pathological role of glycosylation profile of PrP, wild type human PrP (HuPrP) and two genetic engineering generated non-glycosylated PrP mutants (N181Q/N197Q and T183A/T199A) were transiently expressed in human astrocytoma cell line SF126. The results revealed that expressions of non-glycosylated PrP induced significantly more apoptosis cells than that of wild type PrP. It illustrated that Bcl-2 proteins might be involved in the apoptosis pathway of non-glycosylated PrPs. Our data highlights that removal of glycosylation of prion protein provokes cells apoptosis.
Haochi Liu,Lan Ding,Ligang Chen,Yanhua Chen,Tianyu Zhou,Huiyu Li,Yuan Xu,Li Zhao,Ning Huang 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.69 No.-
Biomass carbon dots (CDs) prepared by sweet potato peels were superior fluorophores with low toxicity and excellent photostability. A novel designed fluorescence probe for specific recognition and sensitive detection of oxytetracycline (OTC) was fabricated with CDs and molecularly imprinted polymer (MIP). The quenching of CDs happened when rebinding with OTC due to electron-transfer-induced fluorescence quenching mechanism. The fluorescence probe was successfully applied in honey with the recoveries ranging from 90.2% to 97.3%. The detection limit of OTC was 15.3 ng mL−1. This work provides promising perspectives that the development of fluorescent MIP will be valuable for rapid analysis in complex samples.
Structural and functional analyses of the lipase CinB from <i>Enterobacter asburiae</i>
Shang, Fei,Lan, Jing,Liu, Wei,Chen, Yuanyuan,Wang, Lulu,Zhao, Jing,Chen, Jinli,Gao, Peng,Ha, Nam-Chul,Quan, Chunshan,Nam, Ki Hyun,Xu, Yongbin Elsevier 2019 Biochemical and biophysical research communication Vol.519 No.2
<P><B>Abstract</B></P> <P>Lipases are widely present in various plants, animals and microorganisms, constituting a large category of enzymes. They have the ability to catalyze the cleavage of ester bonds. The lipase CinB from <I>Enterobacter asburiae</I> (<I>E. asburiae</I>) is an acetyl esterase. The primary amino acid sequence suggests that the EaCinB protein belongs to the α/β-hydrolase (ABH) superfamily of the esterase/lipase superfamily. However, its molecular functions have not yet been determined. Here, we report the crystal structure of <I>E. asburiae</I> CinB at a 1.45 Å resolution. EaCinB contains a signal peptide, cap domain and catalytic domain. The active site of EaCinB contains the catalytic triad (Ser180-His307-Asp277) on the catalytic domain. The oxyanion hole is composed of Gly106 and Gly107 within the conserved sequence motif HGGG (amino acid residues 106–109). The substrate is accessible between the α1 and α2 helices or the α1 helix and catalytic domain. Narrow substrate pockets are formed by the α2 helix of the cap domain. Site-directed mutagenesis showed that EaCinB-W208H exhibits a higher catalytic ability than EaCinB-WT by approximately nine times. Our results provide insight into the molecular function of EaCinB.</P> <P><B>Highlights</B></P> <P> <UL> <LI> EaCinB structure contains a signal peptide, cap domain and catalytic domain. </LI> <LI> The active site of EaCinB contains the catalytic triad (Ser180-His307-Asp277). </LI> <LI> The oxyanion hole is composed of Gly106 and Gly107. </LI> <LI> Substrate-binding mode of EaCinB are proposed. </LI> <LI> Site-directed mutagenesis showed that EaCinB-W208H exhibits a higher catalytic ability than EaCinB-WT. </LI> </UL> </P>
Bio-inspired Fabrication of Cu-Ni Coatings onto Mercerized Flax Fabric by Electroless Plating
Lin Zhu,Hang Zhao,Bijian Lan,Lei Hou,Siyi Bi,Yumeng Xu,Yinxiang Lu 한국섬유공학회 2020 Fibers and polymers Vol.21 No.2
This research mainly prepared a bio-friendly electromagnetic interference (EMI) shielding fabric, and discussed the influence of the grammage (i.e. 120 g/m2, 160 g/m2 and 210 g/m2, etc) of fabric substrate on the preparation of metalbased EMI shielding conductive fabric by electroless plating. A series of steps involved mercerization, dopamine (DoPA) modification, Ni0 seeding and electroless copper-nickel (Cu-Ni) plating were carried out to fabricate ideal EMI shielding fabric. The prepared Cu-Ni fabric endowed relatively high EMI shielding effectiveness (SE) (34.80-42.00 dB) ranging from 30 to 4500 MHz, which made it a huge potential in the field of wearable EMI shielding. Moreover, another discovery was that a relative higher grammage may lead to a lower total metal loading. Furthermore, Fourier transformation infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) measurements were conducted to verify the introduction of functional groups in mercerization, modification and activation procedures. The crystalline phases and morphologies of resulting Cu-Ni coatings were characterized by X-ray diffraction (XRD) and field emission-scanning electron microscopy (FE-SEM) measurements. The atomic ratio (at.%) of Cu and Ni in the sample was determined by Energy Dispersive X-ray Spectroscopy (EDX) measurements. Overall, the Cu-Ni alloy fabric prepared in this research met the requirements of health, coating lining and EMI shielding.
Combined effects of three novel SNPs within goat LHX3 gene on milk performance
Jin-Biao Liu,Chu-Zhao Lei,Xian-Yong Lan,Yao Xu,Zhuan-Jian Li,Hong Chen 한국유전학회 2011 Genes & Genomics Vol.33 No.5
In this study, single nucleotide polymorphisms (SNPs) of LHX3 gene were detected by DNA sequencing based on DNA pools and PCR-RFLP method in 792 goats belonging to three Chinese indigenous breeds (Guanzhong dairy, Xinong Sannen dairy, Inner Mongolia white cashmere). The results revealed three novel mutations (AY923832:g.7778A>T; g.8035T>C;g.10592C>T), which were named as P2-DraI, P3-HinfI and P4-MspI loci, respectively. The linkage disequilibrium analysis demonstrated P3-HinfI and P4-MspI loci were strongly linked (r^2>0.33) in all the analyzed populations. Each SNP produced no significant (p>0.05) effects on milk performance. However,the two-loci and three–loci combined genotypes had more profound impacts on milk yield than in separation. The individuals with diplotype AATT (P2-DraI/P3-HinfI) showed significantly (p<0.05) higher milk yield than those with diplotypes ATTT, TTTT, ATTC, and AACC. Diplotype TTCCTT (P2-DraI/P3-HinfI/P4-MspI) carriers had significantly (p<0.05) higher milk yield than those with diplotypes ATTTCC and AACCTC. These combined effects of LHX3 gene polymorphisms indicated that this gene had significant effect on milk performance and its corresponding combined diplotypes could be regarded as potential genetic markers of milk performance.
Hua-Xing Huang,Liang-Lan Shen,Hai-Yan Huang,Li-Hua Zhao,Feng Xu,Dong-Mei Zhang,Xiu-Lin Zhang,Tong Chen,Xue-Qin Wang,Yan Xie,Jian-Bin Su 대한당뇨병학회 2021 Diabetes and Metabolism Journal Vol.45 No.6
Background: Type 2 diabetes mellitus (T2DM) is characterized by elevated fasting glucagon and impaired suppression of postprandial glucagon secretion, which may participate in diabetic complications. Therefore, we investigated the associations of plasma glucagon with estimated glomerular filtration rate (eGFR), albuminuria and diabetic kidney disease (DKD) in T2DM patients.Methods: Fasting glucagon and postchallenge glucagon (assessed by area under the glucagon curve [AUCgla]) levels were determined during oral glucose tolerance tests. Patients with an eGFR <60 mL/min/1.73 m2 and/or a urinary albumin-to-creatinine ratio (UACR) ≥30 mg/g who presented with diabetic retinopathy were identified as having DKD.Results: Of the 2,436 recruited patients, fasting glucagon was correlated with eGFR and UACR (r=–0.112 and r=0.157, respectively; P<0.001), and AUCgla was also correlated with eGFR and UACR (r=–0.267 and r=0.234, respectively; P<0.001). Moreover, 31.7% (n=771) presented with DKD; the prevalence of DKD was 27.3%, 27.6%, 32.5%, and 39.2% in the first (Q1), second (Q2), third (Q3), and fourth quartile (Q4) of fasting glucagon, respectively; and the corresponding prevalence for AUCgla was 25.9%, 22.7%, 33.7%, and 44.4%, respectively. Furthermore, after adjusting for other clinical covariates, the adjusted odds ratios (ORs; 95% confidence intervals) for DKD in Q2, Q3, and Q4 versus Q1 of fasting glucagon were 0.946 (0.697 to 1.284), 1.209 (0.895 to 1.634), and 1.521 (1.129 to 2.049), respectively; the corresponding ORs of AUCgla were 0.825 (0.611 to 1.114), 1.323 (0.989 to 1.769), and 2.066 (1.546 to 2.760), respectively. Additionally, when we restricted our analysis in patients with glycosylated hemoglobin <7.0% (n=471), we found fasting glucagon and AUCgla were still independently associated with DKD.Conclusion: Both increased fasting and postchallenge glucagon levels were independently associated with DKD in T2DM patients.
Effects of Tissue Factor, PAR-2 and MMP-9 Expression on Human Breast Cancer Cell Line MCF-7 Invasion
Lin, Zeng-Mao,Zhao, Jian-Xin,Duan, Xue-Ning,Zhang, Lan-Bo,Ye, Jing-Ming,Xu, Ling,Liu, Yin-Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2
Objective: This study aimed to explore the expression of tissue factor (TF), protease activated receptor-2 (PAR-2), and matrix metalloproteinase-9 (MMP-9) in the MCF-7 breast cancer cell line and influence on invasiveness. Methods: Stable MCF-7 cells transfected with TF cDNA and with TF ShRNA were established. TF, PAR-2, and MMP-9 protein expression was analyzed using indirect immunofluorescence and invasiveness was evaluated using a cell invasion test. Effects of an exogenous PAR-2 agonist were also examined. Results: TF protein expression significantly differed between the TF cDNA and TF ShRNA groups. MMP-9 protein expression was significantly correlated with TF protein expression, but PAR-2 protein expression was unaffected. The PAR-2 agonist significantly enhanced MMP-9 expression and slightly increased TF and PAR-2 expression in the TF ShRNA group, but did not significantly affect protein expression in MCF-7 cells transfected with TF cDNA. TF and MMP-9 expression was positively correlated with the invasiveness of tumor cells. Conclusion: TF, PAR-2, and MMP-9 affect invasiveness of MCF-7 cells. TF may increase MMP-9 expression by activating PAR-2.