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- 저자 : ( Zhou Zhou Zhao ) (검색결과 585 건)
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Metastasis associated genomic aberrations in stage II rectal cancer
Hong Zhao,Zhi-Zhou Shi,Rui Jiang,Dong-Bing Zhao,Hai-Tao Zhou,Jian-Wei Liang,Xin-Yu Bi,Jian-Jun Zhao,Zhi-Yu Li,Jian-Guo Zhou,Zhen Huang,Ye-Fan Zhang,Jian Wang,Xin Xu,Yan Cai,Ming-Rong Wang,Yu Zhang 한국유전학회 2016 Genes & Genomics Vol.38 No.11
Genomic aberrations of rectal carcinoma, especially DNA copy number changes associated with metastasis were largely unclear. We aim to identify the metastasis associated biomarkers in stage II rectal cancer. Formalin-fixed, paraffin-embedded primary tumor tissues of stage II rectal carcinoma were analyzed by array-based comparative genomic hybridization, and genomic aberrations were identified by Genomic Workbench and SAM software. Copy number changes and mRNA expressions were validated by Real-time PCR in an independent rectal cancer samples. The results showed that the most frequent gains in stage II rectal cancer were at 1q21.2-q23.1, 3p21.31, 11q12.2-q23.3, 12q24.11-q24.31, 12q13.11-q14.1 and losses in 18q11.2-q23, 17q21.33-q22, 13q31.1-q31.3, 21q21.1-q21.3, 8p23.3-p23.1 and 4q22.1-q23. Twenty-two amplifications and five homozygous deletions were also identified. We further found that S100A1 (1q21.3-q23.1), MCM7 (7q22.1) and JUND (19p13.11) were amplified and overexpressed in stage II rectal cancer. Interestingly, the genomic aberrations affected 14 signaling pathways including VEGF signaling pathway and fatty acid metabolism. Most importantly, loss of 13q31.1-q34 and gain of 1q44 were associated with distant metastasis. Our results indicated that these metastasis associated genomic changes may be useful to reveal the pathogenesis of rectal cancer metastasis and identify candidate biomarkers.
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Zhou Zhe,Zhang Ming,Xu Mingxi,Zhang Ke,Zhao Yang,Zhou Juan,Zhu Yingjian,Wang Zhong,Lu Mujun 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3
The objective of this study was to investigate whether intraperitoneal incubation improves the regenerative capacity of bladder acellular matrix grafts (BAMGs) in a rat model of bladder augmentation. After 2 weeks of incubation in the peritoneum of male rats, BAMG flaps with vascular pedicles were harvested for autologous bladder augmentation. As the control, BAMGs were directly used for bladder augmentation without intraperitoneal incubation. Histological analyses of the incubated BAMGs demonstrated extensive cell growth and vasculature in homogeneous collagen bundles. The cells were positive for vimentin and negative for α-smooth muscle actin and pan-cytokeratin AE1/AE3. Cystography revealed smoother contours of the augmented bladders in the incubated group at 4 and 12 weeks postoperatively. However, the bladder capacity was not significantly different between the two groups. In both groups, the entire urothelium regenerated well without obvious differences. At both time points, compared with the control group, increased numbers of smooth muscle cells (SMCs) and blood vessels were found in the incubated group. At 12 weeks, the SMCs in the incubated group were more similar to those in the native smooth muscle fiber bundles of the bladder. Taken together, our results demonstrated that BAMGs preincubated in the peritoneum promote the regeneration of bladder smooth muscle via neovascularization in a rat bladder augmentation model.
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Zhao, Yan-Jie,Jiang, Ni,Song, Qing-Kun,Wu, Jiang-Ping,Song, Yu-Guang,Zhang, Hong-Mei,Chen, Feng,Zhou, Lei,Wang, Xiao-Li,Zhou, Xin-Na,Yang, Hua-Bing,Ren, Jun,Lyerly, Herbert Kim Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6
Background: There are few choices for treatment of advanced cancer patients who do not respond to or tolerate conventional anti-cancer treatments. Therefore this study aimed to deploy the benefits and clinical efficacy of continuous dendritic cell-cytokine induced killer cell infusions in such patients. Materials and Methods: A total of 381 infusions (from 67 advanced cases recruited) were included in this study. All patients underwent peripheral blood mononuclear cell apheresis for the following cellular therapy and dendritic cells-cytokine induced killer cells were expanded in vitro. Peripheral blood T lymphocyte subsets were quantified through flow cytometry to address the cellular immunity status. Clinical efficacy and physical activities were evaluated by RECIST criteria and Eastern Cooperative Oncology Group scores respectively. Logistic regression model was used to estimate the association between cellular infusions and clinical benefits. Results: An average of $5.7{\pm}2.94{\times}10^9$ induced cells were infused each time and patients were exposed to 6 infusions. Cellular immunity was improved in that cytotoxic $CD8^+CD28^+$ T lymphocytes were increased by 74% and suppressive $CD8^+CD28^-$ T lymphocytes were elevated by 16% (p<0.05). Continuous infusion of dendritic cells-cytokine induced killer cells was associated with improvement of both patient status and cellular immunity. A median of six infusions were capable of reducing risk of progression by 70% (95%CI 0.10-0.91). Every elevation of one ECOG score corresponded to a 3.90-fold higher progression risk (p<0.05) and 1% increase of $CD8^+CD28^-$ T cell proportion reflecting a 5% higher risk of progression (p<0.05). Conclusions: In advanced cancer patients, continuous dendritic cell-cytokine induced killer cell infusions are capable of recovering cellular immunity, improving patient status and quality of life in those who are unresponsive to conventional cancer treatment.
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Zhao, Li-Jun,Xu, Heng,Qu, Jun-Wei,Zhao, Wan-Zhou,Zhao, Yi-Bing,Wang, Jin-Hua Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8
Objective: To determine whether silence of $PKC-{\alpha}$ expression by small interference RNA (siRNA) might regulate MDR1 expression and reverse chemoresistance of ovarian cancer. Methods: We measured gene and protein expression of MDR1 and $PKC-{\alpha}$ in ovarian cancer cells and assessed their correlation with cell drug resistance. We also examined whether blocking $PKC-{\alpha}$ by RNA interference (RNAi) affected MDR1 expression and reversed drug resistance in drug sensitivity tests. Results: The drug resistance cell lines, OV1228/DDP and OV1228/Taxol, had higher gene and protein expression of MDR1 and $PKC-{\alpha}$ than their counterpart sensitive cell line, OV1228. SiRNA depressed $PKC-{\alpha}$ gene protein expression, as well as MDR1 and protein expression and improved the drug sensitivity in OV1228/DDP and OV1228/Taxol cells. Conclusion: These results indicated that decreasing $PKC-{\alpha}$ expression with siRNA might be an effective method to improve drug sensitivity in drug resistant cells with elevated levels of $PKC-{\alpha}$ and MDR1. A new siRNA-based therapeutic strategy targeting $PKC-{\alpha}$ gene could be designed to overcome the chemoresistance of ovarian cancer.
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NOVEL BENCH-BASED INSPECTION APPROACH FOR AUTOMOBILE ANTI-LOCK BRAKING SYSTEM
Xiangmo Zhao,Ruru Hao,Zhou Zhou,Amira Ashour,Nilanjan Dey 한국자동차공학회 2018 International journal of automotive technology Vol.19 No.5
Bench inspection approach for automobile Anti-lock Braking System (ABS) has gained research interests recently due to its high efficiency, small site occupation and insusceptibility to environment influences. The current work proposed a novel systematic bench inspection approach for ABS. In order to dynamically simulate various road adhesion coefficients, torque controllers are used for loading different torques to the drums. Furthermore, flywheels are adopted to simulate the translational inertia of the vehicle braking on road for compensating the inertial energy of ABS road experiment on the bench. The principal component analysis (PCA) is applied for accurate and efficient data analysis. The automatic evaluation of ABS is achieved by using the processed PCA data as an input to the back-propagation (BP) neural network classifier. The experiments established that the new approach can accurately simulate various road braking conditions. It can be carried out for the inspection of ABS installed in the car.
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Huawei Zhou,Chunju Zhao,Zhipeng Liang,Yihong Zhou,Fang Wang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.8
Spatial positioning of Valid Temperature-measuring Optical Fiber (VTOF) is of great significancefor accurately grasping the real temperature distribution of a concrete dam. However, there is barely an effective way to accurately locate the measuring points of optical fiber in concrete dams. A comprehensive data symmetry analysis technique is presented for determining the optimal spatial positioning of valid temperature-measuring optical fibers (VTOFs) in concrete dams. This approach includes a mathematical model to confirm the center of symmetry in monitoring data, which is required to reconstruct the real spatial temperature distribution. This process can identify VTOF locations and verify the reliability of temperature predictions. Experimental validation was conducted using findings from research on a new mathematical model, site measurements from a concrete dam, and corresponding data from distributed optical fibers. These results were successfully applied to the construction of the Baihetan dam; the concrete temperature from 405 pouring blocks in 5 typical dam monoliths was accurately monitored, and the temperature-measuring points of VTOFs were more than 24711. Meanwhile, the optical fiber temperature measurement and temperature field analysis system of Baihetan dam located in southwest China was developed based on accurately acquired temperature measurement data, which provides data basis and engineering application platform support for temperature evolution and temperature field analysis and precise temperature control management of pouring blocks.
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Huawei Zhou,Yihong Zhou,Chunju Zhao,Fang Wang,Zhipeng Liang 대한토목학회 2018 KSCE Journal of Civil Engineering Vol.22 No.5
Crack prevention is a significant issue in the construction process of concrete dams. The vast majority of concrete cracks arerelated to temperature variations, and hence, temperature control is a primary method used to prevent cracks. This paper presents anew integrated concept, named Feedback Design (FD) of temperature control measures, which incorporates real-time temperaturemonitoring, temperature field simulation and construction process simulation in a large system to optimize a temperature controlscheme for concrete dams. We used a fiber-optic Distributed Temperature Sensing (DTS) system to monitor the temperaturevariation process of typical dam monoliths in real time. By incorporating a genetic algorithm, neural network algorithm, and finiteelement theory, we proposed an intelligent inversion method to obtain thermal parameters of concrete dams based on the temperaturemonitoring data. Meanwhile, we built a simulation model of a concrete dam’s construction process based on a cycling networktechnique to obtain accurate construction environmental parameters. Finally, we simulated the temperature field of a typical dammonolith based on initial temperature control measures, inversion thermal parameters, and accurate construction environmentalparameters. Temperature variance lines of typical points in the model obtained from the finite element method (FEM) accord wellwith the measured values obtained from the DTS system. We applied the FD approach to the Xiluodu arch dam located in southwestChina to forecast the temperature fields and optimize the temperature control scheme of the dam blocks planned for pouring. The FDapproach has been proven reliable and efficacious.
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Temperature Field Reconstruction of Concrete Dams based on Distributed Optical Fiber Monitoring Data
Huawei Zhou,Zhiguo Pan,Zhipeng Liang,Chunju Zhao,Yihong Zhou,Fang Wang 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.5
Mastering the real temperature distribution of the concrete dam is the basis for solving the problem of temperature control and crack prevention. In this paper, Distributed Temperature Sensing (DTS) technology was applied to temperature monitoring of a higharch dam under construction in southwest China. In order to obtain a more comprehensive temperature distribution of the dam, optical fiber layout principles for arch dams were studied, and horizontal and vertical optical fiber layout schemes were first proposed according to these principles. The real temperature variation processes of the dam were obtained in real time with a line temperature measurement pattern instead of a point temperature measurement pattern. Additionally, a framework of distributed optical fiber data acquisition and remote transmission was proposed. Interconnection of multiple DTS hosts and remote transmission of temperature data were realized. Then, two-dimensional temperature fields of a typical dam block in different ages and longitudinal profiles of dam blocks with embedded fibers were reconstructed based on large amounts of temperature monitoring data and the Kriging difference algorithm. Temperature field reconstruction results showed that the temperature distribution law of the concrete arch dam was in accordance with the actual situation.
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