Identification and expression profiling of five chemosensory protein genes in the whitefly MED, Bemisia tabaciIdentification and expression profiling of five chemosensory protein genes in the whitefly MED, Bemisia tabaci

RanWang,Xiao-man Zhang,Hong-liang Li,Xiaojun Guo,Chen Luo 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.1

Insect chemosensory proteins (CSPs) are small, water-soluble proteins involved in detection and recognition of chemical cues and semiochemicals. A better understanding of CSPs and their function could aid in the development of pest control strategies that are based on chemosensory regulation. We screened our unpublished transcriptome data of Bemisia tabaci and identified five CSP genes (BtabCSPs) including two novel BtabCSPs viz. BtabCSP4 and BtabCSP5. Phylogenetic analysis of amino acid sequences of the proteins encoded by these genes revealed that BtabCSPs are distinct fromCSPs of other hemipterans. Quantitative PCR analysis showed differential expression of these genes in different stages. In the adult stage, BtabCSP1, 2 and 4 had significantly higher expression than BtabCSP3 and 5 respectively. On the other hand, BtabCSP2 and 5 were relatively highly expressed in stage of pseudopupae compared to BtabCSP1, 3 and 4. In the third instar BtabCSP3, 4 and 5 exhibited higher expression compared to other CSP genes. Expression of all CSP genes was relatively low in the first and second instars compared to later developmental stages. The identification of novel BtabCSPs and the expression profiling of CSP genes in general may help facilitate development of pest management strategies based on disruption of CSPdriven behaviors.

AMPK alleviates high uric acid-induced Na+-K+-ATPase signaling impairment and cell injury in renal tubules

Jing Xiao,Sibo Zhu,Haochen Guan,Haochen Guan,Fengqin Li,Xiaoli Zhang,Hui Guo,Xiaojun Wang,Zhibin Ye 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

One of the mechanisms in hyperuricemia (HUA)-induced renal tubular injury is the impairment of Na+-K+-ATPase (NKA) signaling, which further triggers inflammation, autophagy, and mitochondrial dysfunction and leads to cell injury. Here, we used RNA sequencing to screen the most likely regulators of NKA signaling and found that the liver kinase B1(LKB1)/adenosine monophosphate (AMP)-activated protein kinase (AMPK)/ mammalian target of rapamycin (mTOR) pathway was the most abundantly enriched pathway in HUA. AMPK is a key regulator of cell energy metabolism; hence, we examined the effect of AMPK on HUA-induced dysregulation of NKA signaling and cell injury. We first detected AMPK activation in high uric acid (UA)-stimulated proximal tubular epithelial cells (PTECs). We further found that sustained treatment with the AMPK activator 5-aminoimidazole-4-carboxamide 1-β-d-ribofuranoside (AICAR), but not the AMPK inhibitor Compound C, significantly alleviated UA-induced reductions in NKA activity and NKA α1 subunit expression on the cell membrane by reducing NKA degradation in lysosomes; sustained AICAR treatment also significantly alleviated activation of the NKA downstream molecules Src and interleukin-1β (IL-1β) in PTECs. AICAR further alleviated high UA-induced apoptosis, autophagy, and mitochondrial dysfunction. Although AMPK activation by metformin did not reduce serum UA levels in hyperuricemic rats, it significantly alleviated HUAinduced renal tubular injury and NKA signaling impairment in vivo with effects similar to those of febuxostat. Our study suggests that AMPK activation may temporarily compensate for HUA-induced renal injury. Sustained AMPK activation could reduce lysosomal NKA degradation and maintain NKA function, thus alleviating NKA downstream inflammation and protecting tubular cells from high UA-induced renal tubular injury.

Electrical heating behavior of flexible thermoplastic polyurethane/ Super-P nanoparticle composite films for advanced wearable heaters

Zhen Xiao,Chengjie Sheng,Yang Xia,Xiaojun Yu,Chu Liang,Hui Huang,Yongping Gan,Jun Zhang,Wenkui Zhang 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.71 No.-

A rationally designedflexible electrothermalfilm is composed of Super-P (SP, nanoparticle),thermoplastic polyurethane (TPU) and silica. The electric heating behavior of electrothermalfilmscan be facilely adjusted by SP contents and applied voltages. TPU/SP composite with 25 wt.% SP presentsrobust structural stability, fast response feature and superior electrothermal reproducibility. Theconductive network formed by SP nanoparticles not only could quickly convert the electric energy toheat, but also is stable under stepwise periodic and long-term electric heating–cooling conditions. Finally, a smart wrist band integrated electric heater and temperature indicator is verified the highpotential in multifunctional wearable device applications.

Data Access Control Scheme Based on Blockchain and Outsourced Verifiable Attribute-Based Encryption in Edge Computing

Chao Ma,Xiaojun Jin,Song Luo,Yifei Wei,Xiao-jun Wang 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.7

The arrival of the Internet of Things and 5G technology enables users to rely on edge computing platforms to process massive data. Data sharing based on edge computing refines the efficiency of data collection and analysis, saves the communication cost of data transmission back and forth, but also causes the privacy leakage of a lot of user data. Based on attribute-based encryption and blockchain technology, we design a fine-grained access control scheme for data in edge computing, which has the characteristics of verifiability, support for outsourcing decryption and user attribute revocation. User attributes are authorized by multi-attribute authorization, and the calculation of outsourcing decryption in attribute encryption is completed by edge server, which reduces the computing cost of end users. Meanwhile, We implemented the user's attribute revocation process through the dual encryption process of attribute authority and blockchain. Compared with other schemes, our scheme can manage users' attributes more flexibly. Blockchain technology also ensures the verifiability in the process of outsourcing decryption, which reduces the space occupied by ciphertext compared with other schemes. Meanwhile, the user attribute revocation scheme realizes the dynamic management of user attribute and protects the privacy of user attribute.

Important Role of Immunological Responses to Environmental Exposure in the Development of Allergic Asthma

Xinliu Lin,Xia Ren,Xiaojun Xiao,Zhaowei Yang,Siyang Yao,Gary WK Wong,Zhigang Liu,Charles Wang,Zhong Su,Jing Li 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.6

Allergic asthma is a public health problem that affects human health and socioeconomic development. Studies have found that the prevalence of asthma has significantly increased in recent years, which has become particularly pronounced in developed countries. With rapid urbanization in China in the last 3 decades, the prevalence of asthma has increased significantly in urban areas. As changes in genetic backgrounds of human populations are limited, environmental exposure may be a major factor that is responsible for the increased prevalence of asthma. This review focuses on environmental components of farms and rural areas that may have protective effects in reducing the development of asthma. Farm and rural related microorganism- and pathogen-associated molecular patterns are considered to be important environmental factors that modulate host's innate and adaptive immune system to induce protection effects later in life. Environmental microbial-related immunotherapy will also be discussed as the future research direction for the prevention of allergic asthma.

Modeling and Analysis of Temperature Compensation for Multitemperature Zone Sintering Furnace Temperature Sensing

Xiaoyue Sang,Zhaohui Yuan,Xiaojun Yu,Muhammad Tariq Sadiq,Na Liang,Noman Naseer,GaoXi Xiao 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.7

The surface temperature of workpieces in a multi-temperature zone sintering furnace is an important parameter to characterize the performances of a multi-temperature zone system. Due to the practical structural properties of the sintering furnace, however, the conventional way of temperature measurement cannot detect the exact surface temperature of the workpieces directly, making it difficult to control the multi-temperature zone system performances precisely. To address such an issue, this paper proposes, for the first time to the best of our knowledge, a temperature compensation algorithm based soft-measurement technique to compensate for the temperature of the measuring points. Specifically, we analyze the heat-transfer mechanism within the electric heating surface and the workpiece surface and establish a mathematical model for it first, and then calculate the radiative heat transfer coefficient between the diffuse gray surface within sintering furnace using the discrete radiation heat transfer method. Finally, the heat transfer mechanism based soft measurement technique is proposed and applied tocompensate for the temperature. Both simulations and experiments with a practical sintering furnace are conducted to verify the correctness and effectiveness of the proposed temperature compensation algorithm in different cases. Results show that the proposed algorithm could help maintain the temperature difference within a range limit of ±5◦, which is much better as compared with the conventional temperature measurement methods.

Sputum Autoantibodies Are More Relevant in Autoimmune Responses in Asthma than Are Serum Autoantibodies

Rundong Qin,Fei Long,Xiaojun Xiao,Jing Xiao,Zhengyu Zheng,Mulin Feng,Renbin Huang,Tao Peng,Jing Li 대한천식알레르기학회 2019 Allergy, Asthma & Immunology Research Vol.11 No.3

Purpose: The data on the differences between sputum autoantibodies (Sp-Abs) and serum autoantibodies (Se-Abs) in reflection of autoimmune responses to lungs is still lacking. Methods: Ten types of Abs were investigated in matched Se and Sp samples collected from recruited subjects. Correlations between Ab levels and airway inflammatory parameters and measures of pulmonary function were assessed. The network-based and inter-correlated analysis was performed to explore the patterns of Sp- and Se-Ab profiles. Results: Fifty stable asthmatic patients and 24 healthy volunteers were recruited for our study, 15 with mild asthma, 18 with moderate asthma and 17 with severe asthma. The concentrations of Sp-Ab against U1 small nuclear ribonucleoprotein (Sp-anti-U1-SnRNP), Sp-Ab against Smith antigen and Se-Ab against thyroid peroxidase (anti-TPO) in severe asthmatics and Sp-anti-U1-SnRNP in moderate asthmatics were significantly higher compared to healthy controls and mild asthmatic subjects (P < 0.05). Sp-anti-U1-SnRNP levels were positively correlated with the dose of inhaled corticosteroids, Sp eosinophil counts and fractional exhaled nitric oxide (r = 0.326, P = 0.022; r = 0.356, P = 0.012; r = 0.241, P = 0.025, respectively) and negatively correlated with Sp neutrophil counts (r = −0.308, P = 0.031) with adjustment for age. Spearman's correlation matrix showed multiple inter-correlations among Sp-Abs and Se-Abs (P < 0.05) while only the levels of Ab against DNA topoisomerase and anti-TPO in Se were correlated with those Sp-Ab counterparts (P < 0.05). The network-based analysis defined 2 clusters: clusters 1 and 2 contained 10 Sp-Abs and 10 Se-Abs, respectively. Conclusions: This study observes that Sp-Abs are more associated with clinical parameters and the severity of disease in asthma compared to Se-Abs. Targeting on Sp-Abs which are the hallmark of the localized autoimmune event might help us better understand the role of autoimmunity in the pathological mechanism of asthma. Keywords: Asthma; autoimmunity; autoantibodies; sputum; se

In-pile tritium release behavior and the post-irradiation experiments of Li4SiO4 fabricated by melting process

Zhao Linjie,Yang Mao,Xiao Chengjian,Gong Yu,Ran Guangming,Chen Xiaojun,Li Jiamao,Yue Lei,Chen Chao,Hou Jingwei,Wang Heyi,Long Xinggui,Peng Shuming 한국원자력학회 2024 Nuclear Engineering and Technology Vol.56 No.1

Understanding the tritium release and retention behavior of candidate tritium breeder materials is crucial for breeder blanket design. Recently, a melt spraying process was developed to prepare Li4SiO4 pebbles, which were subsequently subjected to the in-pile tritium production and extraction platform in China Mianyang Research Reactor (CMRR) to investigate their in-situ tritium release behavior and irradiation performance. The results demonstrate that HT is the main tritium release form, and adding hydrogen to the purge gas reduces tritium retention while increasing the HT percent in the purge gas. Post-irradiation experiments reveal that the irradiated pebbles darken in color and their grains swell, but the mechanical properties remain largely unchanged. It is concluded that the tritium residence time of Li4SiO4 made by melt spraying method at 467 ◦C is approximately 23.34 h. High-density Li4SiO4 pebbles exhibit tritium release at relatively low temperatures (<600 ◦C) that is mainly controlled by bulk diffusion. The diffusion coefficient at 525 ◦C and 550 ◦C is 1.19 × 10 11 cm2/s and 5.34 × 10 11 cm2/s, respectively, with corresponding tritium residence times of 21.3 hours and 4.7 hours.

The Application of Decellularized Adipose Tissue Promotes Wound Healing

Xia Zenan,Guo Xiao,Yu Nanze,Zeng Ang,Si Loubin,Long Fei,Zhang Wenchao,Wang Xiaojun,Zhu Lin,Liu Zhifei 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.6

Background: Due to adipose-derived stem cells (ASCs) being easy to obtain, their rapid proliferation rate, and their multidirectional differentiation capabilities, they have been widely used in the field of regenerative medicine. With the progress of decellularized adipose tissue (DAT) and adipose tissue engineering research, the role of DAT in promoting angiogenesis has gradually been emphasized. Methods: We examined the biological characteristics and biosafety of DAT and evaluated the stem cell maintenance ability and promotion of growth factor secretion through conducting in vitro and in vivo studies. Results The tested ASCs showed high rat:es of proliferation and adhered well to DAT. The expression levels of essential genes for cell stem maintenance, including OCT4, SOX2, and Nanog were low at 2–24 h and much higher at 48 and 96 h. The Adipogenic expression level of markers for ASCs proliferation including PPARγ, C/EPBα, and LPL increased from 2 to 96 h. Co-culture of ASCs and DAT increased the secretion of local growth factors, such as VEGF, PDGF-bb, bFGF, HGF, EGF, and FDGF-bb, and secretion gradually increased from 0 to 48 h. A model of full-thickness skin defects on the back of nude mice was established, and the co-culture of ASCs and DAT showed the best in vivo treatment effect. Conclusion: The application of DAT promotes wound healing, and DAT combined with ASCs may be a promising material in adipose tissue engineering and regenerative medicine.

Experimental and Numerical Research on the Laser Impact Welding of Ti/SS

Jiaxin Lu,Huixia Liu,Hao Jin,Feng Li,Xiaojun Wang,Xiao Wang 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.5

The welding of Ti/SS metal foil was realized by laser impact welding. The interface morphology and mechanical properties of welding specimens were studied by systematic experiments, and the experimental phenomena were revealed by smoothed particle hydrodynamics numerical simulation. Results indicated that the plastic deformation of the composite plate and the springback of the welding spot center were small under low laser pulse energy and enlarged under high laser pulse energy. Impinging jet, interface wave, and interface cracking were found at the micro-interface of the welding specimens. Increasing the laser pulse energy resulted in the transition of the interface waveform from straight to microwave and finally to wave primarily because of the change in interfacial impact pressure and effective plastic strain. Energy dispersive spectroscopy analysis showed that the trace element diff used at the welding interface, and no intermetallic compounds were formed. SPH simulation revealed that the thickness of the high-temperature layer was related to the thickness of the diffusion layer. As the distance between the test point and welding interface increased, the hardness decreased. The maximum tensile force of the welding specimens increased with laser pulse energy. The failure mode of the interface was welding spot edge fracture failure.