The beneficial effect of ginsenosides extracted by pulsed electric field against hydrogen peroxide-induced oxidative stress in HEK-293 cells

Liu, Di,Zhang, Ting,Chen, Zhifei,Wang, Ying,Ma, Shuang,Liu, Jiyun,Liu, Jingbo The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.2

Background: Ginsenosides are the main pharmacological components of Panax ginseng root, which are thought to be primarily responsible for the suppressing effect on oxidative stress. Methods: 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and oxygen radical absorption capacity were applied to evaluate the antioxidant activities of the ginsenosides. Human embryonic kidney 293 (HEK-293) cells were incubated with ginsenosides extracted by pulsed electric field (PEF) and solvent cold soak extraction (SCSE) for 24 h and then the injury was induced by $40{\mu}M$ $H_2O_2$. The cell viability and surface morphology of HEK-293 cells were studied using MTS assay and scanning electron microscopy, respectively. Dichloro-dihydro-fluorescein diacetate fluorescent probe assay was used to measure the level of intracellular reactive oxygen species. The intracellular antioxidant activities of ginsenosides were evaluated by cellular antioxidant activity assay in HepG2 cells. Results: The PEF extracts displayed the higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and stronger oxygen radical absorption capacity (with an oxygen radical absorption capacity value of $14.48{\pm}4.04{\mu}M\;TE\;per\;{\mu}g/mL$). The HEK-293 cell model also suggested that the protective effect of PEF extracts was dose-dependently greater than SCSE extracts. Dichloro-dihydro-fluorescein diacetate assay further proved that PEF extracts are more active (8% higher than SCSE extracts) in reducing intracellular reactive oxygen species accumulation. In addition, scanning electron microscopy images showed that the HEK-293 cells, which were treated with PEF extracts, maintained more intact surface morphology. Cellular antioxidant activity values indicated that ginsenosides extracted by PEF had stronger cellular antioxidant activity than SCSE ginsenosides extracts. Conclusion: The present study demonstrated the antioxidative effect of ginsenosides extracted by PEF in vitro. Furthermore, rather than SCSE, PEF may be more useful as an alternative extraction technique for the extraction of ginsenosides with enhanced antioxidant activity.

Spatio-temporal expression pattern and fasting response of follistatin gene in teleost Larimichthys crocea

Zhifei Liu,Liangyi Xue,Weiliang Shen,Jun Ying,Zhenhua Zhang 한국유전학회 2014 Genes & Genomics Vol.36 No.2

Follistatin is a multifunctional regulatory proteininvolved in numerous physiological activities. Tobetter understand its functions in Larimichthys crocea (L.crocea), the entire cDNA sequence of follistatin from L. crocea was cloned, the transcript abundance of follistatinwas measured at different development stages and in differentgender. A fasting-refeeding protocol was also performedto correlate follistatin tissue expression withnutritional status in the juvenile stage of L. crocea. Theresults showed that follistatin transcripts were weaklydetected before gastrula stage (9 h post-fertilization; hpf),and at the highest level at myogenesis stage (15 hpf) and 30days after hatching (30 dah). In adult fish, follistatin transcriptswere observed in all the ten tissues examined andmost abundant in eye, gill, muscle, brain and intestine. Moreover, the abundance of follistatin transcripts in mosttissues was influenced by the age and gender. Duringfasting and refeeding period, the regulatory response offollistatin in muscle, liver, gonad, spleen and brain of L. crocea varied greatly. Altogether, these findings supportthe evidence that follistatin plays a profound role in theteleost development and that it is an indispensable factorfor the survival of teleosts when challenged with environmentalstresses like fasting.

Myostatin-2 isolation and spatiotemporal expression comparison between myostatin-1 and -2 in Larimichthys crocea

Zhifei Liu,Liangyi Xue,Sheng Sun,Zhen Xu,Hong Yu 한국유전학회 2014 Genes & Genomics Vol.36 No.5

The 3,110-bp myostatin-2 was obtained from amarine teleost, Larimichthys crocea (L. crocea), by PCRamplication coupled with 50- and 30-rapid amplification ofcDNA ends. Myostatin-2 contains a 71-bp 50-untranslatedregion, a 328-bp exon I, an 895-bp intron I, a 371-bp exonII, a 382-bp intron II, a 381-bp exon III, and a 682 bp 30-untranslated region, and it encodes a protein of 359 aminoacid residues. The deduced amino acid sequence has typicalcharacteristics of TGF-b family members with nineconserved cysteine residues and a RXXR proteolytic processingsite within the conserved C-terminal portion, andmore than 90 % similarity with other fish myostatin-2proteins. Quantitative assessment revealed that levels ofmyostatin-1 transcripts fluctuated markedly, whereas myostatin-2 expression remained very low during most stagesof embryonic development. In addition, both myostatin-1and -2 transcripts were detected in all 10 examined tissuesof juvenile and adult individuals, but they exhibited differentexpression patterns and responses to fasting. Expression of myostatin-1 was highest in skeletal muscle,which was higher than myostatin-2 by 2–3 orders ofmagnitude. Myostatin-2 transcripts were greatest in brain. Myostatin-1 expression increased significantly after 3 daysof fasting, whereas myostatin-2 expression was unaffected. Our results imply that myostatin-1 and -2 may have differentroles in skeletal muscle growth and development ofL. crocea.

The beneficial effect of ginsenosides extracted by pulsed electric field against hydrogen peroxide-induced oxidative stress in HEK-293 cells

Di Liu,Ting Zhang,Zhifei Chen,Ying Wang,Shuang Ma,Jiyun Liu,Jingbo Liu 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.2

Background: Ginsenosides are the main pharmacological components of Panax ginseng root, which are thought to be primarily responsible for the suppressing effect on oxidative stress. Methods: 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and oxygen radical absorption capacity were applied to evaluate the antioxidant activities of the ginsenosides. Human embryonic kidney 293 (HEK-293) cells were incubated with ginsenosides extracted by pulsed electric field (PEF) and solvent cold soak extraction (SCSE) for 24 h and then the injury was induced by 40mM H2O2. The cell viability and surface morphology of HEK-293 cells were studied using MTS assay and scanning electron microscopy, respectively. Dichloro-dihydro-fluorescein diacetate fluorescent probe assay was used to measure the level of intracellular reactive oxygen species. The intracellular antioxidant activities of ginsenosides were evaluated by cellular antioxidant activity assay in HepG2 cells. Results: The PEF extracts displayed the higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and stronger oxygen radical absorption capacity (with an oxygen radical absorption capacity value of 14.48 4.04mM TE per mg/mL). The HEK-293 cell model also suggested that the protective effect of PEF extracts was dose-dependently greater than SCSE extracts. Dichloro-dihydro-fluorescein diacetate assay further proved that PEF extracts are more active (8% higher than SCSE extracts) in reducing intracellular reactive oxygen species accumulation. In addition, scanning electron microscopy images showed that the HEK-293 cells, which were treated with PEF extracts, maintained more intact surface morphology. Cellular antioxidant activity values indicated that ginsenosides extracted by PEF had stronger cellular antioxidant activity than SCSE ginsenosides extracts. Conclusion: The present study demonstrated the antioxidative effect of ginsenosides extracted by PEF in vitro. Furthermore, rather than SCSE, PEF may be more useful as an alternative extraction technique for the extraction of ginsenosides with enhanced antioxidant activity.

Note on the Shipai biota (Cambrian series 2, stage 4) yielded from a new section (Xiachazhuang) in the Maoping town of Zigui county, western Hubei province, south China

Fan Liu,Feiyang Chen,Yanlong Chen,Zhifei Zhang 대한지질학회 2017 대한지질학회 학술대회 Vol.2017 No.10

Performance Improvement of Model Predictive Control Using Control Error Compensation for Power Electronic Converters Based on the Lyapunov Function

Du, Guiping,Liu, Zhifei,Du, Fada,Li, Jiajian The Korean Institute of Power Electronics 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.4

This paper proposes a model predictive control based on the discrete Lyapunov function to improve the performance of power electronic converters. The proposed control technique, based on the finite control set model predictive control (FCS-MPC), defines a cost function for the control law which is determined under the Lyapunov stability theorem with a control error compensation. The steady state and dynamic performance of the proposed control strategy has been tested under a single phase AC/DC voltage source rectifier (S-VSR). Experimental results demonstrate that the proposed control strategy not only offers global stability and good robustness but also leads to a high quality sinusoidal current with a reasonably low total harmonic distortion (THD) and a fast dynamic response under linear loads.

Performance Improvement of Model Predictive Control Using Control Error Compensation for Power Electronic Converters Based on the Lyapunov Function

Guiping Du,Zhifei Liu,Fada Du,Jiajian Li 전력전자학회 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.4

This paper proposes a model predictive control based on the discrete Lyapunov function to improve the performance of power electronic converters. The proposed control technique, based on the finite control set model predictive control (FCS-MPC), defines a cost function for the control law which is determined under the Lyapunov stability theorem with a control error compensation. The steady state and dynamic performance of the proposed control strategy has been tested under a single phase AC/DC voltage source rectifier (S-VSR). Experimental results demonstrate that the proposed control strategy not only offers global stability and good robustness but also leads to a high quality sinusoidal current with a reasonably low total harmonic distortion (THD) and a fast dynamic response under linear loads.

Suberoylanilide hydroxamic acid suppresses axonal damage and neurological dysfunction after subarachnoid hemorrhage via the HDAC1/HSP70/TDP-43 axis

Luo Kui,Wang Zhifei,Zhuang Kai,Yuan Shishan,Liu Fei,Liu Aihua 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Increased focus has been placed on the role of histone deacetylase inhibitors as crucial players in subarachnoid hemorrhage (SAH) progression. Therefore, this study was designed to expand the understanding of SAH by exploring the downstream mechanism of the histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) in SAH. The expression of TDP-43 in patients with SAH and rat models of SAH was measured. Then, western blot analysis, immunofluorescence staining, and transmission electron microscope were used to investigate the in vitro effect of TDP-43 on a neuronal cell model of SAH established by oxyhemoglobin treatment. Immunofluorescence staining and coimmunoprecipitation assays were conducted to explore the relationship among histone deacetylase 1 (HDAC1), heat shock protein 70 (HSP70), and TDP-43. Furthermore, the in vivo effect of HDAC1 on SAH was investigated in rat models of SAH established by endovascular perforation. High expression of TDP-43 in the cerebrospinal fluid of patients with SAH and brain tissues of rat models of SAH was observed, and TDP-43 accumulation in the cytoplasm and the formation of inclusion bodies were responsible for axonal damage, abnormal nuclear membrane morphology, and apoptosis in neurons. TDP-43 degradation was promoted by the HDAC1 inhibitor SAHA via the acetylation of HSP70, alleviating SAH, and this effect was verified in vivo in rat models. In conclusion, SAHA relieved axonal damage and neurological dysfunction after SAH via the HSP70 acetylation-induced degradation of TDP-43, highlighting a novel therapeutic target for SAH.

Acoustic emission characterization and cracking characteristics of reinforced concrete pipe

Jianfeng Li,Linbing Wang,Dongcan Chen,Yalin Liu,Zhifei Gao,Wentao Wang,Hailu Yang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.9

Acoustic emission (AE) and digital image correlation (DIC) technology were used in this study to monitor the behavior of reinforced concrete pipe (RCP) in the optimized three-edge bearing test (TEBT). At the same time, the AE source location of different pipe sections was simulated. The results show that the whole compressive loading process for RCP could be divided into 3 to 4 stages. Both the AE counts and AE energy could well characterize the cracking of concrete, and AE energy was more sensitive to the breaking of steel bar. The amplitude and duration of AE signal produced by the breaking of steel bar were larger than those produced by the cracking of concrete. The AE frequency for concrete cracking was relatively scattered. The AE frequency for steel bar breaking was small and concentrated, and this frequency distribution showed an exponential decreasing trend. The results of optimized AE source location showed that the generation and development of cracks occur in the initial loading stage. The results of multi-index analysis showed that the cracks in the top (loading position) and the bottom (bearing position) of the RCP show that the cracks extend from the inner wall to the outer wall, while the cracks on the left and right sides perpendicular to the loading direction show that the cracks extend from the outer wall to the inner wall.

The Application of Decellularized Adipose Tissue Promotes Wound Healing

Xia Zenan,Guo Xiao,Yu Nanze,Zeng Ang,Si Loubin,Long Fei,Zhang Wenchao,Wang Xiaojun,Zhu Lin,Liu Zhifei 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.6

Background: Due to adipose-derived stem cells (ASCs) being easy to obtain, their rapid proliferation rate, and their multidirectional differentiation capabilities, they have been widely used in the field of regenerative medicine. With the progress of decellularized adipose tissue (DAT) and adipose tissue engineering research, the role of DAT in promoting angiogenesis has gradually been emphasized. Methods: We examined the biological characteristics and biosafety of DAT and evaluated the stem cell maintenance ability and promotion of growth factor secretion through conducting in vitro and in vivo studies. Results The tested ASCs showed high rat:es of proliferation and adhered well to DAT. The expression levels of essential genes for cell stem maintenance, including OCT4, SOX2, and Nanog were low at 2–24 h and much higher at 48 and 96 h. The Adipogenic expression level of markers for ASCs proliferation including PPARγ, C/EPBα, and LPL increased from 2 to 96 h. Co-culture of ASCs and DAT increased the secretion of local growth factors, such as VEGF, PDGF-bb, bFGF, HGF, EGF, and FDGF-bb, and secretion gradually increased from 0 to 48 h. A model of full-thickness skin defects on the back of nude mice was established, and the co-culture of ASCs and DAT showed the best in vivo treatment effect. Conclusion: The application of DAT promotes wound healing, and DAT combined with ASCs may be a promising material in adipose tissue engineering and regenerative medicine.