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Xiaojing Zhang,Nan Zhang,Yue Zhou,Robert Delatolla,Yali Song,Yongpeng Ma,Hongzhong Zhang 대한환경공학회 2020 Environmental Engineering Research Vol.25 No.6
In this study, the effect of CuO nanoparticles (NPs) on partial nitrification (PN) process was investigated at various pH values. The short- and long-term experiments were carried out in six identical reactors, with or without CuO NPs, at pH values of 6.5, 8.0 and 10.0. The ammonia oxidation, reaction rates, copper content, and the microbial community were investigated. The results of this work suggested that CuO NPs inhibited the ammonia oxidation by aerobic ammonia-oxidizing bacteria (AOB), under both the acid and alkali conditions. AOB could resist the acid condition but was significantly suppressed when CuO NPs was fed, and the low pH did not aggravate the inhibition level. Almost all the bacteria lost bioactivity under the pH as 10.0, while anaerobic ammonia-oxidizing bacteria survived. The acid condition increased the Nitrosomonas relative abundance while the alkali condition lowered it. More than 60% of the supplied CuO NPs was discharged via effluent.
Xu Xiaojing,Zhou Yijun,Wei Shanjun,Ren Dongtao,Yang Min,Bu Huahu,Kang Mingming,Wang Junli,Feng Jinchao 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.4
Superoxide dismutases (SODs) constitute the first line of cellular defense against oxidative stress in plants. SODs generally occur in three different forms with Cu/Zn, Fe, or Mn as prosthetic metals. We cloned the full-length cDNA of the Thellungiella halophila Cu/Zn-SOD gene ThCSD using degenerate RT-PCR and rapid amplification of cDNA ends (RACE). Sequence analysis indicated that the ThCSD gene (GenBank accession number EF405867) had an open reading frame of 456 bp. The deduced 152-amino acid polypeptide had a predicted molecular weight of 15.1 kDa, an estimated pI of 5.4, and a putative Cu/Zn-binding site. Recombinant ThCSD protein was expressed in Escherichia coli and assayed for SOD enzymatic activity in a native polyacrylamide gel. The SOD activity of ThCSD was inactivated by potassium cyanide and hydrogen peroxide but not by sodium azide, confirming that ThCSD is a Cu/Zn-SOD. Northern blotting demonstrated that ThCSD is expressed in roots, stems, and leaves. ThCSD mRNA levels increased by about 30-fold when plants were treated with sodium chloride (NaCl), abscisic acid (ABA), and indole-acetic acid (IAA) and by about 50-fold when treated with UVB light. These results indicate that ThCSD is involved in physiological pathways activated by a variety of environmental conditions.
Study on the splitting failure of the surrounding rock of underground caverns
Li, Xiaojing,Chen, Han-Mei,Sun, Yanbo,Zhou, Rongxin,Wang, Lige Techno-Press 2018 Geomechanics & engineering Vol.14 No.5
In this paper splitting failure on rock pillars among the underground caverns has been studied. The damaged structure is considered to be thin plates and then the failure mechanism of rock pillars has been studied consequently. The critical load of buckling failure of the rock plate has also been obtained. Furthermore, with a combination of the basic energy dissipation principle, generalized formulas in estimating the number of splitting cracks and in predicting the maximum deflection of thin plate have been proposed. The splitting criterion and the mechanical model proposed in this paper are finally verified with numerical calculations in FLAC 3D.
Proteomic Analysis on Exosomes Derived from Patients’ Sera Infected with Echinococcus granulosus
Wen Wang,Xiaojing Zhou,Fang Cui,Chunli Shi,Yulan Wang,Yanfei Men,Wei Zhao,Jiaqing Zhao 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.5
Cystic echinococcosis (CE), a zoonotic disease caused by Echinococcus granulosus at the larval stage, predominantly develops in the liver and lungs of intermediate hosts and eventually results in organ malfunction or even death. The interaction between E. granulosus and human body is incompletely understood. Exosomes are nanosized particles ubiquitously present in human body fluids. Exosomes carry biomolecules that facilitate communication between cells. To the best of our knowledge, the role of exosomes in patients with CE is not reported. Here, we isolated exosomes from the sera of patients with CE (CE-exo) and healthy donors and subjected them to liquid chromatography-tandem mass spectrometry analysis. Proteomic analysis identified 49 proteins specifically expressed in CE-exo, including 4 proteins of parasitic origin. The most valuable parasitic proteins included tubulin alpha-1C chain and histone H4. And 8 proteins were differentially regulated in CE-exo (fold change>1.5), as analyzed with bioinformatic methods such as annotation and functional enrichment analyses. These findings may improve our understanding about the interaction between E. granulosus and human body, and may contribute to the diagnosis and prevention of CE.
Le Yang,Xiaojing Zou,Qiansheng Liang,Hao Chen,Jun Feng,Li Yan,Zhaohua Wang,Daixing Zhou,Shusheng Li,Shanglong Yao,Zhi Zheng 생화학분자생물학회 2007 Experimental and molecular medicine Vol.39 No.1
Cardiomyocyte hypertrophy is a major cause of morbidity and mortality worldwide. The aim of this study is to determine the effects of sodium tans - hinone IIA sulfonate (STS) on cardiomyocyte hypertrophy induced by angiotensin II (Ang II) in vivo and in vitro. In long-term treatment, adult Wistar rats were infused with Ang II for three weeks via osmotic mini-pumps and some of them were given intragas - trically of STS. Left ventricle was isolated; the ratio of left ventricular weight to body weight and systolic blood pressure (SBP) were determined and heart morphometry was assessed after hematoxylin and eosin staining. Results indicated STS inhibited Ang II-induced increases in myocyte diameter and decreased the LVW/BW ratio independent of decreasing systolic blood pressure. In vitro, treatment of cultured cardiomyocytes with STS inhibited Ang II-induced increase in cell size, protein synthesis, ANP expression, activation of extracellular signalregulated kinase (ERK) and ERK kinase (MEK). Then we reexamined the mechanism of STS-induced antihypertrophic effects. Results revealed MEK inhibitor U0126 (20 µM) markedly enhanced STS-induced depressions in [3H]leucine incorporation and ANP expression. In conclusion, MEK/ERK pathway plays a significant role in the anti-hypertrophic effects of STS.
HCBP6 upregulates human SREBP1c expression by binding to C/EBPβ-binding site in the SREBP1c promoter
( Xueliang Yang ),( Ming Han ),( Shunai Liu ),( Xiaoxue Yuan ),( Xiaojing Liu ),( Shenghu Feng ),( Li Zhou ),( Yaru Li ),( Hongping Lu ),( Jun Cheng ),( Shumei Lin ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.1
Sterol regulatory element-binding protein-1c (SREBP1c) plays an important role in triglyceride (TG) homeostasis. Although our previous study showed that hepatitis C virus core-binding protein 6 (HCBP6) regulates SREBP1c expression to maintain intracellular TG homeostasis, the mechanism underlying this regulation is unclear. In the present study, we found that HCBP6 increased intracellular TG levels by upregulating SREBP1c expression. HCBP6 increased SREBP1c transcription by directly binding to the SREBP1c promoter (at the -139- to +359-bp region). Moreover, we observed that HCBP6 interacted with C/EBPβ-binding site in the SREBP1c promoter both in vitro and in vivo. These results indicate that HCBP6 upregulates human SREBP1c expression by binding to the C/EBPβ-binding site in the SREBP1c promoter. [BMB Reports 2018; 51(1): 33-38]
Ningmei Chen,Buerbatu Song,Shuai Tang,Junqing He,Yijun Zhou,Jinchao Feng,Sha Shi,Xiaojing Xu 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.5
The cuticle, composed primarily of wax and cutin, covers most plant aerial surfaces and plays a vital role in interactions between plants and their environment. Some ATP-binding cassette G subfamily (ABCG) members are involved in cuticular lipid molecule exportation to outside in the plant surface. Thellungiella salsugineum, a relative of Arabidopsis thaliana with a heavy cuticle, has extreme stress tolerance. TsABCG11, an ABCG transporter was cloned (GenBank accession number JQ389853), and its structure was studied. qRT-PCR showed that TsABCG11 expression varied in different organs of T. salsugineum and was upregulated under ABA, NaCl, drought and cold conditions. The rosette leaves from 4-week-old TsABCG11 overexpressed (OE) Arabidopsis plants displayed lower rates of water loss and decreased chlorophyll-extracted rates compared to wild-type plants. TsABCG11-OE plants also exhibited significantly increased total cuticular wax and cutin monomer amounts, mainly due to prominent changes in the C29, C31, and C33 alkanes in the wax and C18:2 dioic in cutin monomers, respectively. TsABCG11-OE seedlings exhibit lower root growth inhibition under 100 mM of NaCl or 1 μM of ABA than the wild type. Four-week-old TsABCG11-OE plants exhibited higher photosynthetic rates and water-use efficiency under cold stress (4 °C) than control plants. These results indicate that TsABCG11 plays an important role in cuticle lipid exportation and is involved in abiotic stresses, probably having a close relationship with extreme stress tolerance in T. salsugineum.
Antifreeze protein detection using Rhodamine B as photoluminescence label in porous silicon
Hongyan Zhang,Zhenhong Jia,Xiaoyi Lv,Junwei Hou,Xiaojing Liu,Ji Ma,Jun Zhou 한국물리학회 2013 Current Applied Physics Vol.13 No.4
A novel method is demonstrated to detect Antifreeze proteins (AFPs) based on photoluminescence (PL)using porous silicon (PS) coated with silver as a substrate. Ag/PS substrate is obtained through immersion of PS in silver nitrate (AgNO3) solutions and is incubated with Rhodamine B (RB) as PL label. This substrate is easy to be fabricated and the pore size of PS is large enough for biological molecules to infiltrate, which is an ideal platform for biological molecule detection. Through functionalization used glutaraldehyde (GTA) and 4-(N-Maleimidomethyl) cyclohexane-1-carboxylicacid (Sulfo-SMCC) as crosslinkers separately, we test the role of the AFPs antibodies in selective capturing the AFPs antigen and explain the reason of the enhancement of PL intensity. The result shows a significant enhancement of the PL intensity of RB at around 590 nm due to the interaction of antibodyeantigen competitive binding with AFPs. Therefore, the PL corresponding to RB was selected to detect the target AFPs and the PL intensity of RB proportional to the AFPs concentration. The detection limit was found to be 1.65 mg/ml for AFPs when GTA was used as cross-linker, and the detection limit was 16.5 ng/ml with Sulfo-SMCC as cross-linker
( Lixiang Chen ),( Cong Wang ),( Shun Li ),( Xin Yu ),( Xue Liu ),( Rongrong Ren ),( Wenwen Liu ),( Xiaojing Zhou ),( Xiaonan Zhang ),( Xiaohui Zhou ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.4
Chlamydiae, obligate intracellular bacteria, are associated with a variety of human diseases. The chlamydial life cycle undergoes a biphasic development: replicative reticulate bodies (RBs) phase and infectious elementary bodies (EBs) phase. At the end of the chlamydial intracellular life cycle, EBs have to be released to the surrounded cells. Therefore, the interactions between Chlamydiae and cell death pathways could greatly influence the outcomes of Chlamydia infection. However, the underlying molecular mechanisms remain elusive. Here, we investigated host cell death after Chlamydia infection in vitro, in L929 cells, and showed that Chlamydia infection induces cell necrosis, as detected by the propidium iodide (PI)-Annexin V double-staining flow-cytometric assay and Lactate dehydrogenase (LDH) release assay. The production of reactive oxygen species (ROS), an important factor in induction of necrosis, was increased after Chlamydia infection, and inhibition of ROS with specific pharmacological inhibitors, diphenylene iodonium (DPI) or butylated hydroxyanisole (BHA), led to significant suppression of necrosis. Interestingly, live-cell imaging revealed that Chlamydia infection induced lysosome membrane permeabilization (LMP). When an inhibitor upstream of LMP, CA-074-Me, was added to cells, the production of ROS was reduced with concomitant inhibition of necrosis. Taken together, our results indicate that Chlamydia infection elicits the production of ROS, which is dependent on LMP at least partially, followed by induction of host-cell necrosis. To our best knowledge, this is the first live-cell-imaging observation of LMP post Chlamydia infection and report on the link of LMP to ROS to necrosis during Chlamydia infection.