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( Xiaojing Fan ),( Ruixian Yang ),( Sixin Qiu ),( Xueqing Cai ),( Huasong Zou ),( Fangping Hu ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.5
The eglS gene in Bacillus amyloliquefaciens encodes an endo-β-1,4-glucanase that belongs to glycosyl hydrolase family 5. In this study, a disruption mutant of gene eglS was constructed to examine its role in bacterial adaptation in plants. The mutant TB2k, eglS gene inactivated bacterial strain, was remarkably impaired in extracellular cellulase activity. When inoculated on Brassica campestris, the TB2k population was reduced by more than 60% compared with the wild-type strain in the root, stem, and leaf tissues. Overexpression of eglS in the wild-type strain increased the bacteria population in the plant tissues. Further studies revealed that the transcription level of eglS was correlated with bacterial population. These data demonstrate that endo-β-1,4-glucanase of B. amyloliquefaciens is required for its optimal endophytic colonization.
Le Yang,Xiaojing Zou,Qiansheng Liang,Hao Chen,Jun Feng,Li Yan,Zhaohua Wang,Daixing Zhou,Shusheng Li,Shanglong Yao,Zhi Zheng 생화학분자생물학회 2007 Experimental and molecular medicine Vol.39 No.1
Cardiomyocyte hypertrophy is a major cause of morbidity and mortality worldwide. The aim of this study is to determine the effects of sodium tans - hinone IIA sulfonate (STS) on cardiomyocyte hypertrophy induced by angiotensin II (Ang II) in vivo and in vitro. In long-term treatment, adult Wistar rats were infused with Ang II for three weeks via osmotic mini-pumps and some of them were given intragas - trically of STS. Left ventricle was isolated; the ratio of left ventricular weight to body weight and systolic blood pressure (SBP) were determined and heart morphometry was assessed after hematoxylin and eosin staining. Results indicated STS inhibited Ang II-induced increases in myocyte diameter and decreased the LVW/BW ratio independent of decreasing systolic blood pressure. In vitro, treatment of cultured cardiomyocytes with STS inhibited Ang II-induced increase in cell size, protein synthesis, ANP expression, activation of extracellular signalregulated kinase (ERK) and ERK kinase (MEK). Then we reexamined the mechanism of STS-induced antihypertrophic effects. Results revealed MEK inhibitor U0126 (20 µM) markedly enhanced STS-induced depressions in [3H]leucine incorporation and ANP expression. In conclusion, MEK/ERK pathway plays a significant role in the anti-hypertrophic effects of STS.
Effects of a chitosan nanoparticles encapsulation on the properties of litchi polyphenols
Xingan Cheng,Qiwen Zou,Hanhui Zhang,Jianwei Zhu,Murtaza Hasan,Fangyun Dong,Xin Liu,Junjie Li,Yuehua Wu,Xiaojing Lv,Keqiang Wang,Xiangling Deng,Zhanmei Liu,Xuhong Jiang 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.13
Litchi polyphenols have very specific biological activities. Nevertheless, the low and inconsistent oral bioavailability and instability hinder the further application of litchi polyphenols in food systems. This work prepared litchi polyphenols loaded chitosan nanoparticles (LP-CSNPs) by ionic gelation method to enhance the encapsulation on the properties of litchi polyphenols. The optimum conditions of formation via single factors and the Box–Behnken design were chitosan (CS) concentration 1.065 mg/mL, sodium tripolyphosphate (TPP) concentration 0.975 mg/mL, and the mass ratios of polyphenols and CS 1:1 with encapsulation efficiency (EE%) of 45.53%. LP-CSNPs presented the nanosized range of particle size (mean 170 nm), excellent polydispersity index (PDI) (0.156 ± 0.025), and zeta potential values (+ 35.44 ± 0.59). The in vitro release in simulated gastric fluid (pH 1.2) and intestinal fluid (pH 6.8) during 100 h was 58.34% and 81.68%, respectively. LP-CSNPs could effectively improve the storage stability and had great antibacterial activity compared with unencapsulated litchi polyphenols.