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Yang, Xiao-Li,Zhang, Cheng-Dong,Wu, Hua-Yu,Wu, Yong-Hu,Zhang, Yue-Ning,Qin, Meng-Bin,Wu, Hua,Liu, Xiao-Chun,Lina, Xing,Lu, Shao-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11
Trichostatin A (TSA) is a histone deacetylase (HDAC) inhibitor. We here investigated its effects on proliferation and apoptosis of the CNE2 carcinoma cell line, and attempted to establish genome-wide DNA methylation alteration due to differentially histone acetylation status. After cells were treated by TSA, the inhibitory rate of cell proliferation was examined with a CCK8 kit, and cell apoptosis was determined by flow cytometry. Compared to control, TSA inhibited CNE2 cell growth and induced apoptosis. Furthermore, TSA was found to induce genome-wide methylation alteration as assessed by genome-wide methylation array. Overall DNA methylation level of cells treated with TSA was higher than in controls. Function and pathway analysis revealed that many genes with methylation alteration were involved in key biological roles, such as apoptosis and cell proliferation. Three genes (DAP3, HSPB1 and CLDN) were independently confirmed by quantitative real-time PCR. Finally, we conclude that TSA inhibits CNE2 cell growth and induces apoptosis in vitro involving genome-wide DNA methylation alteration, so that it has promising application prospects in treatment of NPC in vivo. Although many unreported hypermethylated/hypomethylated genes should be further analyzed and validated, the pointers to new biomarkers and therapeutic strategies in the treatment of NPC should be stressed.
Meng-Jie Hu,Yan-Wu Wang,Jiang-Wen Xiao 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.3
This paper is concerned with the problem of positive observer design for impulsive positive systems(IPS) with interval uncertainties and time delay. A copositive Lyapunov-Krasovskii functional with exponentialterm is constructed. By applying the average impulsive interval method, sufficient conditions for the existence ofthe positive observer are established to guarantee the exponential stability of the corresponding augmented system,which ensures the designed positive observer can estimate the system states exponentially. Combined with the linearprogramming (LP) technique, an algorithm is developed to design the observer gain matrices. Finally, a numericalexample is provided to show the effectiveness of the theoretical results.
Meng, Fa-Yan,Jiang, Bing-Li,Lin, Cui-Wu,Wang, Li,Tan, Xiao-He Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.3
Two new benzimidazolyl-containing complexes have been synthesized by reactions of $Cu^{II}$ salts and 1,2-bis(benzimidazolyl) benzene ($H_2bbbz$) with two different dicarboxylate ligands. When phthalate acid ($H_2pt$) was employed as secondary ligand, a 0D molecular complex Cu$(H_2bbbz)(pt){\cdot}(H_2pt)$ (1)was furnished and when the secondary ligand was instead by a linear bridging ligand of terephthalic acid ($H_2tp$) a 1D zipper-like coordination polymer $[Cu(H_2bbbz)(tp){\cdot}2(C_2H_5OH){\cdot}H_2O]_n$ (2) was obtained, suggesting the structure-direction effect of the secondary dicarbxylate ligand. The preliminary investigation on the spectral properties of the complexes was also presented.
Xiao Gang Weng,Quan Jiang Song,Qiong Wu,Ming Chao Liu,Meng Ling Wang,Jiu Feng Wang 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.4
To acquire epidemiological data on the bovine viral diarrhea virus (BVDV) and identify cattle persistently infected (PI) with this virus, 4,327 samples from Holstein dairy cows were screened over a four-year period in Beijing, China. Eighteen BVD viruses were isolated, 12 from PI cattle. Based on genetic analysis of their 5’-untranslated region (5’-UTR), the 18 isolates were assigned to subgenotype BVDV-1m, 1a, 1d, 1q, and 1b. To investigate the innate immune responses in the peripheral-blood mononuclear cells of PI cattle, the expression of Toll-like receptors (TLRs), RIG-I-like receptors, interferon-a (IFN-a), IFN-b, myxovirus (influenza virus) resistance 1 (MX1), and interferon stimulatory gene 15 (ISG15) was assessed by qPCR. When compared with healthy cattle, the expression of TLR-7, IFN-a, and IFN-b mRNA was downregulated, but the expression of MX1 and ISG-15 mRNA was upregulated in PI cattle. Immunoblotting analysis revealed that the expression of interferon regulatory factor 3 (IRF-3) and IRF-7 was lower in PI cattle than in healthy cattle. Thus, BVDV-1m and 1a are the predominant subgenotypes in the Beijing region, and the strains are highly divergent. Our findings also suggest that the TLR-7/IRF-7 signaling pathway plays a role in evasion of host restriction by BVDV.
Wu Yu-Xiao,Fei Zi-Jian,Feng De-Cheng,Song Meng-Yan 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.12
After a loss of coolant accident (LOCA) in the prestressed concrete containment vessels (PCCVs) of nuclear power plants, the coupling of temperature and pressure can significantly affect the mechanical properties of the PCCVs. However, there is no consensus on how this coupling affects the failure mechanism of PCCVs. In this paper, a simplified finite element modeling method is proposed to study the effect of temperature and pressure coupling on PCCVs. The experiment results of a 1:4 scale PCCV model tested at Sandia National Laboratory (SNL) are compared with the results obtained from the proposed modeling approach. Seven working conditions are set up by varying the internal and external temperatures to investigate the failure mechanism of the PCCV model under the coupling effect of temperature and pressure. The results of this paper demonstrate that the finite element model established by the simplified finite element method proposed in this paper is highly consistent with the experimental results. Furthermore, the stress-displacement curve of the PCCV during loading can be divided into four stages, each of which corresponds to the damage to the concrete, steel liner, steel rebar, and prestressing tendon. Finally, the failure mechanism of the PCCV is significantly affected by temperature.
Luo Xiao-Jing,He Ming-Ming,Liu Jia,Zheng Jia-Bo,Wu Qi-Nian,Chen Yan-Xing,Meng Qi,Luo Kong-Jia,Chen Dong-Liang,Xu Rui-Hua,Zeng Zhao-Lei,Liu Ze-Xian,Luo Hui-Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Esophageal squamous cell carcinoma (ESCC) is one of the most life- and health-threatening malignant diseases worldwide, especially in China. Long noncoding RNAs (lncRNAs) have emerged as important regulators of tumorigenesis and tumor progression. However, the roles and mechanisms of lncRNAs in ESCC require further exploration. Here, in combination with a small interfering RNA (siRNA) library targeting specific lncRNAs, we performed MTS and Transwell assays to screen functional lncRNAs that were overexpressed in ESCC. TMPO-AS1 expression was significantly upregulated in ESCC tumor samples, with higher TMPOAS1 expression positively correlated with shorter overall survival times. In vitro and in vivo functional experiments revealed that TMPO-AS1 promotes the proliferation and metastasis of ESCC cells. Mechanistically, TMPO-AS1 bound to fused in sarcoma (FUS) and recruited p300 to the TMPO promoter, forming biomolecular condensates in situ to activate TMPO transcription in cis by increasing the acetylation of histone H3 lysine 27 (H3K27ac). Targeting TMPO-AS1 led to impaired ESCC tumor growth in a patient-derived xenograft (PDX) model. We found that TMPO-AS1 is required for cell proliferation and metastasis in ESCC by promoting the expression of TMPO, and both TMPO-AS1 and TMPO might be potential biomarkers and therapeutic targets in ESCC.
Peipei Wu,Shumei Wang,Qicai Chen,Lili Chen,Pengpeng Zhang,Juan Xiao,Xiaoxiao Chen,Meng Liu 연세대학교의과대학 2017 Yonsei medical journal Vol.58 No.1
Purpose: Elevation in serum alanine aminotransferase (ALT) levels is a biomarker for metabolic syndrome (MS); however, the relationshiphas not been fully investigated within the reference interval of ALT levels. Our objective was to explore the relationship between serum ALT levels within the reference interval and MS in Chinese adults. Materials and Methods: This cross-sectional study included 16028 adults, who attended routine health check-ups at Shengli OilfieldCentral Hospital from January 2006 to March 2012. The reference interval of serum ALT level was defined as less than 40 U/L. Logistic regression models and restricted cubic spline were used to evaluate the association of ALT with MS. Results: The prevalence of MS in the total population was 13.7% (6.4% for females and 18.4% for males). Multiple logistic regressionshowed that ALT levels were positively associated with MS after adjustment for potential confounding factors. The odds ratio of MS in the top quartile was 4.830 [95% confidence interval (CI): 2.980–7.829] in females and 3.168 (95% CI: 2.649–3.790) in males, compared with the ALT levels in the bottom quartile. The restricted cubic spline models revealed a positive non-linear dose-response relationship between ALT levels and the risk of MS in women (p for nonlinearity was 0.0327), but a positive linear dose-response relationship in men (p for nonlinearity was 0.0659). Conclusion: Serum ALT levels within the reference interval are positively associated with MS in a dose-response manner. ElevatedALT levels, even within the reference interval, may reflect early dysmetabolic changes.