LncRNA TMPO-AS1 promotes esophageal squamous cell carcinoma progression by forming biomolecular condensates with FUS and p300 to regulate TMPO transcription

Luo Xiao-Jing,He Ming-Ming,Liu Jia,Zheng Jia-Bo,Wu Qi-Nian,Chen Yan-Xing,Meng Qi,Luo Kong-Jia,Chen Dong-Liang,Xu Rui-Hua,Zeng Zhao-Lei,Liu Ze-Xian,Luo Hui-Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Esophageal squamous cell carcinoma (ESCC) is one of the most life- and health-threatening malignant diseases worldwide, especially in China. Long noncoding RNAs (lncRNAs) have emerged as important regulators of tumorigenesis and tumor progression. However, the roles and mechanisms of lncRNAs in ESCC require further exploration. Here, in combination with a small interfering RNA (siRNA) library targeting specific lncRNAs, we performed MTS and Transwell assays to screen functional lncRNAs that were overexpressed in ESCC. TMPO-AS1 expression was significantly upregulated in ESCC tumor samples, with higher TMPOAS1 expression positively correlated with shorter overall survival times. In vitro and in vivo functional experiments revealed that TMPO-AS1 promotes the proliferation and metastasis of ESCC cells. Mechanistically, TMPO-AS1 bound to fused in sarcoma (FUS) and recruited p300 to the TMPO promoter, forming biomolecular condensates in situ to activate TMPO transcription in cis by increasing the acetylation of histone H3 lysine 27 (H3K27ac). Targeting TMPO-AS1 led to impaired ESCC tumor growth in a patient-derived xenograft (PDX) model. We found that TMPO-AS1 is required for cell proliferation and metastasis in ESCC by promoting the expression of TMPO, and both TMPO-AS1 and TMPO might be potential biomarkers and therapeutic targets in ESCC.

Wireless Network based Formation Control for Multiple Agents

Jing Yan,Xian Yang,Xiao-yuan Luo,Xin-Ping Guan,Chang-chun Hua 제어·로봇·시스템학회 2014 International Journal of Control, Automation, and Vol.12 No.2

Motivated by the potentially application merits of wireless networked control systems (WNCSs), this paper presents a network-based formation framework for multi-agent systems. First, we construct a discrete formation protocol by integrating 802.11b. Meanwhile, one agent is considered as the reference, and the other ones keep a desired formation with the reference via communication net-works. Using Lyapunov stability theory, sufficient convergence conditions are derived, and the verifi-cation problem of the sufficient conditions can be transformed as a linear matrix inequality (LMI) feasibility problem. Solving this LMI feasibility problem, a networked-based feedback controller can be easily obtained. Finally, simulation studies are provided to demonstrate the effectiveness of the pro-posed method.

Metabolism Characteristics of Mycoplasma pneumoniae Infection in Asthmatic Children

Luo Jiaying,Chen Huian,Zhang Qiyong,Huang Xinyun,Qin Xu,Li Jing,Chen Siyi,Xiao Yongxin,Sun Lihong,Sun Baoqing 대한천식알레르기학회 2022 Allergy, Asthma & Immunology Research Vol.14 No.6

Purpose: Studies have shown that Mycoplasma pneumoniae (Mp) infection can aggravate symptoms in asthmatics. However, the mechanism by which Mp infection exacerbates asthma remains unclear. Metabolomics can help identify the mechanism of Mp aggravating asthma in children, thereby providing more a potential target for improving clinical treatment programs. In this article, we analyzed the metabolic level of patients to explain how Mp aggravates asthma in children. Methods: We divided the subjects into the asthma, Mp infection, asthma combined with Mp infection and healthy groups. Patients’ peripheral blood was collected for metabolic and interaction analysis. Cytokine levels were measured via serum and exhaled breath condensate (EBC). Results: A total of 150 participating subjects were divided into four groups after exclusion. We found out that there were different metabolic pathways between the healthy and disease groups. The major pathways of both asthma and asthma combined with Mp infection were valine, leucine and isoleucine biosynthesis; malate-aspartate shuttle was the main differential pathway for Mp infection. Moreover, even though three disease groups involved 81 metabolites at the same time, compared with asthma combined with Mp infection, 2 single disease groups still involved different amino acid pathways (phenylalanine, tyrosine and tryptophan biosynthesis; valine, leucine and isoleucine biosynthesis). Interaction analysis showed that Mp infection in asthmatic patients not only activated cytokines, but also activated Toll-like receptors (TLRs) 2 and 6. Finally, the levels of interleukin (IL)-4, IL-8, IL-13 and tumor necrosis factor-α in EBC with asthma combined with Mp infection were significantly higher than the 2 single disease groups. Conclusions: Mp infection in asthmatic children can cause changes in the levels of various amino acids in the body, which were enriched in the pathways such as valine, leucine and isoleucine biosynthesis. Palmitic acid can activate TLR2, and iloprost reduces IL-10 levels, ultimately leading to the increased airway inflammation.

Glulathione-S-transferases Gene Polymorphism in Prediction of Gastric Cancer Risk by Smoking and Helicobacter Pylori Infection Status

Jing, Chen,Huang, Zhi-Jie,Duan, Yu-Qin,Wang, Pei-Hong,Zhang, Ru,Luo, Ke-Shu,Xiao, Xin-Rong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Aim: To evaluate the association of glutathione S-transferases gene polymorphisms with the risk of gastric cancer, with reference to smoking and Helicobacter pylori infection. Methods: We conducted a 1:1 matched case-control study with 410 gastric cancer cases and 410 cancer-free controls. Polymorphisms of GSTM1, GSTT1 and GSTP1 were determined using PCR-CTPP. Results: The GSTM1 and GSTT1 null genotypes were significantly associated with the risk of gastric cancer after adjusting for potential confounding factors (OR=1.68, 95% CI=1.32-2.23 for null GSTM1, OR=1.73; 95% CI=1.24-2.13 for null GSTT1). The combination of null GSTM1 and null GSTT1 conferred an elevated risk (OR=2.54, 95% CI=1.55-3.39). However, no association was found for GSTP1 polymorphism The smoking modified the association of GSTM1 and GSTT1 null genotypes with the risk of gastric cancer. Conclusion: GSTM1 and GSTT1 null genotypes are associated with increased risk of gastric cancer, and smoking modifies the association.

Knockdown of GCF2/LRRFIP1 by RNAi Causes Cell Growth Inhibition and Increased Apoptosis in Human Hepatoma HepG2 Cells

Li, Jing-Ping,Cao, Nai-Xia,Jiang, Ri-Ting,He, Shao-Jian,Huang, Tian-Ming,Wu, Bo,Chen, De-Feng,Ma, Ping,Chen, Li,Zhou, Su-Fang,Xie, Xiao-Xun,Luo, Guo-Rong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Background: GC-binding factor 2 (GCF2) is a transcriptional regulator that represses transcriptional activity of the epidermal growth factor receptor (EGFR) by binding to a specific GC-rich sequence in the EGFR gene promoter. In addition to this function, GCF2 has also been identified as a tumor-associated antigen and regarded as a potentially valuable serum biomarker for early human hepatocellular carcinoma (HCC) diagnosis. GCF2 is high expressed in most HCC tissues and cell lines including HepG2. This study focused on the influence of GCF2 on cell proliferation and apoptosis in HepG2 cells. Materials and Methods: GCF2 expression at both mRNA and protein levels in HepG2 cells was detected with reverse transcription (RT) PCR and Western blotting, respectively. RNA interference (RNAi) technology was used to knock down GCF2 mRNA and protein expression. Afterwards, cell viability was analyzed with a Cell Counting Kit-8 (CCK-8), and cell apoptosis and caspase 3 activity by flow cytometry and with a Caspase 3 Activity Kit, respectively. Results: Specific down-regulation of GCF2 expression caused cell growth inhibition, and increased apoptosis and caspase 3 activity in HepG2 cells. Conclusions: These primary results suggest that GCF2 may influence cell proliferation and apoptosis in HepG2 cells, and also provides a molecular basis for further investigation into the possible mechanism at proliferation and apoptosis in HCC.

Overexpression of TRPM7 is Associated with Poor Prognosis in Human Ovarian Carcinoma

Wang, Jing,Xiao, Ling,Luo, Chen-Hui,Zhou, Hui,Hu, Jun,Tang, Yu-Xi,Fang, Kai-Ning,Zhang, Yi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

Background: The melastatin-related transient receptor potential 7 channel (TRPM7) is a nonselective cation channel that has been shown to promote tumor metastasis and progression. In this study, we determined the expression of TRPM7 in ovarian carcinomas and investigated its possible prognostic value. Materials and Methods: Samples were collected from 138 patients with ovarian cancer. Expression of TRPM7 was assessed by real-time PCR and immunohistochemistry, expressed with reference to an established scoring system and related to clinical pathological factors. Kaplan-Meier survival analysis was applied to estimate disease-free survival (DFS) and overall survival (OS). Univariate and multivariate cox regression analyses were performed to correlate TRPM7 expression levels with DFS and OS. Results: TRPM7 was highly expressed in ovarian carcinoma and significantly associated with decreased disease-free survival (DFS: median 20 months vs. 42 months, P=0.0002) and overall survival (OS: median 27 months vs. 46 months, P<0.001). Conclusion: Overexpression of TRPM7 expression is significantly associated with poor prognosis in patients with ovarian cancer.

Corrosion behavior of refractory metals in liquid lead at 1000 °C for 1000 h

Zunqi Xiao,Jing Liu,Zhizhong Jiang,Lin Luo 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.6

Lead-based fast reactor (LFR) has become one of the most promising reactors for Generation IV nuclearsystems. A developing trend of LFR is high efficiency, along with operation temperatures up to 800 C oreven higher. One of key issues in the high-efficiency LFR is corrosion of cladding materials with lead athigh temperatures. In this study, corrosion behavior of some refractory metals (Nb, Nb521, and Mo-0.5La)was investigated in static lead at 1000 C for 1000 h. The results showed that Nb and Nb521 exhibited anintense dissolution corrosion with obvious lead penetration after corrosion, and lead penetrationextended along the grain boundaries of the specimens. Furthermore, Nb521 showed a better corrosionresistance than that of Nb as a result of the elements of W and Mo included in Nb521. Mo-0.5La showedmuch better corrosion resistance than that of Nb and Nb521, and no lead penetration could be observed. However, an etched morphology appeared on the surface of Mo-0.5La, indicating the occurrence ofcorrosion to a certain degree. The results indicate that Mo-0.5La is compatible with lead up to 1000 C. While Nb and

Corrosion behavior and mechanism of CLAM and 316L steels in fl owing Pb – 17Li alloy under magnetic field

Zunqi Xiao,Jing Liu,Zhizhong Jiang,Lin Luo,Qunying Huang 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.6

The liquid lead-lithium (Pbe17Li) blanket has many applications in fusion reactors due to its good tritiumbreeding performance, high heat transfer efficiency and safety. The compatibility of liquid Pbe17Li alloywith the structural material of blanket under magnetic field is one of the concerns. In this study,corrosion experiments China low activation martensitic (CLAM) steel and 316L steel were carried out in aforced convection Pbe17Li loop under 1.0 T magnetic field at 480 C for 1000 h. The corrosion results on316L steel showed the characteristic with a superficial porous layer resulted from selective leaching ofhigh-soluble alloy elements and subsequent phase transformation from austenitic matrix to ferriticphase. Then the porous layers were eroded by high-velocity jet fluid. The main corrosion mechanism ofCLAM steel was selective dissolution-base corrosion attack on the microstructure boundary regions andexclusively on high residual stress areas. CLAM steel performed a better corrosion resistance than that of316L steel. The high Ni dissolution rate and the erosion of corroded layers are the main causes for thesevere corrosion of 316L steel.

Systematic Evaluation and Meta-Analysis of the Effect of Gynostemma pentaphyllum on Clinical Indexes of Hyperlipidemia

Hao-Tian Luo,Man-Yu Xiao,Wen-Jing Pei,Kang-Le Bi,Peng Xie,Yu-Long Gu,Xiang-Lan Piao 한국생약학회 2023 Natural Product Sciences Vol.29 No.4

Consensus Tracking for Teleoperating Cyber-physical System

Lingmin Zhang,Jing Yan,Xian Yang,Xiao-yuan Luo,Fuxiao Tan,Xinbin Li 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.3

In this paper, we investigate the consensus tracking problem for teleoperating cyber-physical system (TCPS) under time-varying delay and actuator saturation constraints. Due to the introduction of communication network, variable propagation time delays are considered in cyber element. In addition, actuator saturation problem is investigated in physical element, which is caused by the physical restrictions on robots. Then, a novel distributed consensus tracking controller is proposed in the presence of time-varying delay and actuator saturation. Meanwhile, sufficient conditions are provided to show that the consensus tracking controller can stabilize the master-slave TCPS. Finally, simulations and experiments are performed to validate our proposed results. It is shown that the consensus controllers can guarantee the asymptotic stability of single-master-multi-slave TCPS.