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- 검색키워드
- 저자 : Wang Hai-Zhen (검색결과 31 건)
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Wang Hai-Zhen,Zhang Ru-yi,Yuyu-Li,Liu Xin 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2
Gynaephora qinghaiensis is a pest in the alpine meadows of the Qinghai-Tibet Plateau (QTP) that seriously harms grassland vegetation. Sexual dimorphism (SD) is common in insects, but its molecular mechanism at the tran scriptome level in insects, especially in G. qinghaiensis, has not been reported. In this study, we conducted transcriptome analysis of pupae and adults of the two sexes (male and female) of G. qinghaiensis. A total of 204,557,900 clean reads assembled into 114,944 unigenes were obtained by RNA-Seq. To explore differences in the transcriptome levels of molecular characteristics related to SD between the two sexes of G. qinghaiensis, gene expression profiling was performed. A total of 88 differentially expressed unigenes related to sex determination were found in the pupae of G. qinghaiensis; of these, 74 were upregulated and 14 were downregulated in the female pupae compared to the male pupae. Forty-five unigenes related to sex determination were found in the adults of G. qinghaiensis; of these, 15 were upregulated and 30 were downregulated in the female adults compared to the male adults. This indicated some differences in the expression of genes involved in sex deter mination between male and female individuals of G. qinghaiensis. Therefore, we speculated that sex-determining genes may play an important role in the formation of sexual dimorphism in G. qinghaiensis. Our report provides a valuable genomic resource for further studies of grassland caterpillars and improves our understanding of the molecular mechanisms underlying sexual dimorphism between male and female insects.
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Hai-Zhen Wang,Xin Zhong,Gu-Ren Zhang,Xin Liu,Li Gu 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2
Gynaephora qinghaiensis is a pest on the Qinghai-Tibet Plateau (QTP) that has led to substantial destruction of grassland vegetation. Its pupae are also natural hosts of parasitic wasp. Sexual dimorphism in immune responses is prevalent in vertebrates and invertebrates. However, sexual dimorphism in immune responses of insects, particularly G. qinghaiensis, is poorly understood at the transcriptional level. Here, we performed transcriptome sequencing in male and female pupae of G. qinghaiensis. A total of 118,357,040 clean reads were obtained and assembled into 114,944 unigenes. To explore the difference of immune responses in pupae of both sexes (male and female) of G. qinghaiensis. based on transcriptional level, we characterized the expression profiles of candidate transcripts in the two sexes of G. qinghaiensis. A total of 3,469 unigenes (1,888 up-regulated and 1,581 down-regulated genes) were differentially expressed in pupae of both sexes. Among the differentially expressed genes (DEGs), 263 unigenes related to immune responses were found in the two sexes in G. qinghaiensis; of these, 202 were up-regulated and 61 were down-regulated in the female pupae compared to male pupae. This indicates there were some differences in the expression of immune-related genes between male and female G. qinghaiensis pupae. Therefore, we speculated that the differences in immune responses also likely exsited in pupae of both sexes. Our report provides a valuable genomic resource for further studies of Gynaephora and improves our understanding of the molecular mechanisms underlying immunological differences between male and female insects.
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Hai-Yan Zhang,Zhen-Xian Du,Bao-Qin Liu,Yan-Yan Gao,Xin Meng,Yifu Guan,Wei-Wei Deng,Hua-Qin Wang 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.5
TNF-related apoptosis-inducing ligand (TRAIL) has been proposed as a promising cancer therapy that preferentially induces apoptosis in cancer cells, but not most normal tissues. However, many cancers are resistant to TRAIL by mechanisms that are poorly understood. In this study, we showed that tunicamycin, a naturally occurring antibiotic, was a potent enhancer of TRAIL-induced apoptosis through downregulation of survivin. The tunicamycin-mediated sensitization to TRAIL was efficiently reduced by forced expression of survivin, suggesting that the sensitization was mediated at least in part through inhibition of survivin expression. Tunicamycin also repressed expression of cyclin D1, a cell cycle regulator commonly overexpressed in thyroid carcinoma. Furthermore, silencing cyclin D1 by RNA interference reduced survivin expression and sensitized thyroid cancer cells to TRAIL; in contrast, forced expression of cyclin D1 attenuated tunicamycin-potentiated TRAIL-induced apoptosis via over-riding downregulation of survivin. Collectively, our results demonstrated that tunicamycin promoted TRAIL-induced apoptosis, at least in part, by inhibiting the expression of cyclin D1 and subsequent survivin. Of note, tunicamycin did not sensitize the differentiated thyroid epithelial cells to TRAIL-induced apoptosis. Thus, combined treatment with tunicamycin and TRAIL may offer an attractive strategy for safely treating resistant thyroid cancers. TNF-related apoptosis-inducing ligand (TRAIL) has been proposed as a promising cancer therapy that preferentially induces apoptosis in cancer cells, but not most normal tissues. However, many cancers are resistant to TRAIL by mechanisms that are poorly understood. In this study, we showed that tunicamycin, a naturally occurring antibiotic, was a potent enhancer of TRAIL-induced apoptosis through downregulation of survivin. The tunicamycin-mediated sensitization to TRAIL was efficiently reduced by forced expression of survivin, suggesting that the sensitization was mediated at least in part through inhibition of survivin expression. Tunicamycin also repressed expression of cyclin D1, a cell cycle regulator commonly overexpressed in thyroid carcinoma. Furthermore, silencing cyclin D1 by RNA interference reduced survivin expression and sensitized thyroid cancer cells to TRAIL; in contrast, forced expression of cyclin D1 attenuated tunicamycin-potentiated TRAIL-induced apoptosis via over-riding downregulation of survivin. Collectively, our results demonstrated that tunicamycin promoted TRAIL-induced apoptosis, at least in part, by inhibiting the expression of cyclin D1 and subsequent survivin. Of note, tunicamycin did not sensitize the differentiated thyroid epithelial cells to TRAIL-induced apoptosis. Thus, combined treatment with tunicamycin and TRAIL may offer an attractive strategy for safely treating resistant thyroid cancers.
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Design and Analysis of an Active Balancing Mechanism for a Vertical Axis Washing Machine
Hai-Wei Chen,Xun-Ting Yuan,Zhen Sun,Qiu-Qi Mou,Ming Xiong,Wei-Hua Wang 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.23 No.7
In recent years, active balancing of washing machines has drawn much attention from manufactures. This paper proposes a novel active balancing mechanism for vibration suppression of a vertical axis washing machine which employs water for counteracting the unbalanced laundry. At first, a novel structure with two balancing planes sharing only one set of nozzles for injecting water is designed and the balancing capability of the structure is analyzed. Then, a dynamic model of the active balancing system is constructed. Vibration characteristics of the system including changes of the vibration amplitude and phase angle under different influences are studied. After that, a strategy for identifying the imbalance is proposed and an active control method is designed. At last, a washing machine armed with the active mechanism is manufactured. Both a fixed eccentric block and a pile of randomly distributed laundry are used to test the balancing effect. The results show that lateral vibrations of the washer can be greatly reduced by the active mechanism. Due to the simplicity of the structure and the satisfactory balancing effect, the mechanism proposed has a big potential to be employed in future washing machines.
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Zhen-Xian Du,Hai-Yan Zhang,Da-Xin Gao,Hua-Qin Wang,Yong-Jun Li,Guo-Liang Liu 생화학분자생물학회 2006 Experimental and molecular medicine Vol.38 No.3
apoptosis protein (IAP) family, which is known to be over-expressed in various carcinomas and associated with their biologically aggressive characteristics. The aim of this study was to investigate survivin expression in human medullary thyroid carcinoma (MTC) and a MTC cell line TT, correlate suvivin expression with clinicopathologic features of MTC, and test effects of antisurvivin oligonucleotides (ASODNs) on growth and apoptosis of TT cells. Survivin expression was immunohistochemically determined in formalin-fixed and paraffinembedded specimens obtained from 10 cases of normal thyroid (NT) and 10 cases of MTC, and in TT cells. In TT cells, we confirmed survivin expression and its down-regulation by ASODNs using RT-PCR and Western blot analyses, and investigated effects of ASODNs on viability and growth by MTT assay and apoptosis by apoptotic analyses including DNA laddering assay, acridine orange/ethidium bromide staining and flow cytometric cell cycle analysis. Immunohistochemical analysis showed high survivin expression in MTC and TT cells, whereas no immunoreactivity was detectable in NT. Statistical analyses revealed no significant correlation of survivin expression with the clinicopathologic features of MTC. In TT cells, survivin expression at both mRNA and protein levels was confirmed and could be down-regulated by ASODNs concomitant with decrease in viability and growth, and increase in apoptosis. Our results suggest that survivin plays an important role in MTC independent of the conventional clinicopathologic factors, and ASODNs is a promising survivin- targeted gene therapy for MTC.
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Hai-zhen Zhao,Juan Wang,Fengxia Lv,Xiaomei Bie,Zhaoxin Lu 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.2
A water-soluble exopolysaccharide (PESP-1) was extracted and purified using DEAE Sephadex A-50 and Sephadex G-100 columns from a submerged culture broth of Pholiota squarrosa Quel. AS 5.245. PEPS-1 was investigated for antitumor activity against Heps tumors implanted in mice. An inhibition rate of 78.46% at a dosage of 100 mg/kg was observed. PEPS-1 significantly (p<0.05) increased the relative spleen/thymus indices of Heps tumor-bearing mice at a dosage of 100 mg/kg, compared with controls. Antitumor properties were probably related to stimulation of the immune response. Preliminary physicochemical analysis identified PEPS-1 as a heteropolysaccharide mainly containing D-mannose, D-glucose and D-galactose at molar ratios of 50:33:18. Small amounts of D-rhamnose and D-xylose were also detected. The average Mw of PEPS-1 was 3.26×104 Da. Structural features probably played an important role in the antitumor activity of PEPS-1.
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Construction and Expression of an Eukaryotic Expression Vector Containing the IER3 Gene
Wang, Zhen,Yu, Hong-Sheng,Yao, Ru-Yong,Qiu, Wen-Sheng,Yue, Lu,Sui, Ai-Hua,Liu, Xiang-Ping,Liu, Shi-Hai Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1
Background: More and more research indicate that the immediately early response gene 3 (IER3) is involved inmany biological provesses, such as apoptosis and immunoreaction, as well as viral infection, tumorigenesis and tumour progression. Methods: Here we describe the construction of an eukaryotic expression vector containing IER3 gene and its expression in A549 cells as assessed through fluorescence microscopyand Western-blotting. Results: Fluorescence detection displayed that GFP in cytoplasm was high during 48 and 72 hours post-transfection. In addition, Western blotting showed significant increase in IER3 gene expression in the transfected cells compared with controls. Conclusion: The recombinate plasmid expression vector was constructed successfully, which may provide a basis for further exploration of function of IER3 in lung cancer.
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Wang Jin-Jin,Shao Meng-Meng,Zhou Xue-Zhen,Lin Zu-Chen,Li Hai-Yan,Yuan Zheng-Zhong,Ma Qin-Qin,Li Fang,Fu Wen-Bin 사단법인약침학회 2024 Journal of Acupuncture & Meridian Studies Vol.17 No.3
Importance: Post-stroke sialorrhea (PSS) refers to excessive saliva flowing out the lip border after a stroke. PSS negatively affects patient self-image and social communication and may lead to depression. Limited evidence supports the link between excessive salivation and PSS. No large-scale, strictly controlled randomized controlled trials have shown the effectiveness of acupuncture in treating PSS patients. Objective: We aim to compare the effects of intraoral and sham acupuncture in PSS patients and explore relationships among salivation and drooling severity and frequency and swallowing function in stroke patients. Design: Clinical study protocol, SPIRIT compliant. Setting: Prospective, single-center, randomized, and sham-controlled trial. Population: We will recruit 106 PSS patients to receive 4-week intraoral or sham acupuncture. Additionally, 53 stroke patients without PSS will undergo a conventional 4-week treatment program to compare salivation between PSS and non-PSS patients. Exposures: Intraoral or sham acupuncture. Main Outcomes and Measures: The main evaluation index will be the 3-minute saliva weight (3MSW), comparing changes in 3MSW from baseline to weeks 4 and 8. Secondary assessment indices will include the “Drooling Severity and Frequency Scale” and “Functional Oral Intake Scale.” Results: The results from this study will be published in peer-reviewed journals. Conclusion: Comparing effects of intraoral and sham acupuncture in PSS patients, this study may contribute important evidence for future PSS treatment and provide valuable insights into whether salivation issues in stroke patients are attributed to heightened salivary secretion or dysphagia.
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Automatic balancing of a flexible supported rotor with two disks by two ball balancers
Hai-Wei Chen,Yan-Bin Chen,Zhen Sun,Zhi Wang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.7
This paper studies automatic balancing of a flexible supported rotor with two disks by two ball balancers. Firstly, reduced dynamic descriptions of different components in the rotor are derived using a special method according to Lagrangian equation, based on which a general vibration model of the rotor system is then constructed. Secondly, equations governing frequency responses of the rotor are derived and working principles of the balls in the rotor are illustrated based on basic dynamic phenomena. Thirdly, an autonomous model of the rotor system is built with equations governing movement of the balls derived. Three equilibrium solutions are discovered whose dynamic characteristics are then studied using numerical bifurcation analysis method. Finally, locations of the balls at the steady state are observed using a high speed camera, and the capability of the two balancers for automatic balancing under different conditions is validated.
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