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Acetate와 Glucose가 혼합되어 있는 배지에서 성장하는 당근 현탁배양 세포의 diauxic growth
李宅堅,李宇成 성균관대학교 기초과학연구소 1995 論文集 Vol.46 No.2
우리는 이제까지 bacteria와 yeast 등에서 관찰되어 온 diauxic growth 현상이 식물에서도 관찰되는지에 대해 연구해왔다. 우리는 이미 단자엽(벼) 현탁배양을 acetate와 glucose 가 혼합되어 있는 배지에서 성장시켰을 때 diauxic growth 현상이 있음을 보고하였다 (1). 우리는 쌍자엽식물에서도 diauxic growth가 관찰되는지 알아보기 위하여 당근의 현탁배양을 이용하여 연구하였다. 당근의 현탁배양세포를 glucose와 acetate가 혼합되어 들어있는 배지에서 성장시켰을 때 diauxic growth가 관찰되었다. Diauxic growth의 첫 번째 성장기에 acetate를 사용하고 두 번째 성장기에 glucose를 사용한다. 첫 번째 성장기에 있는 세포는 [^14C]acetate를 흡수하지만 [^14C]glucose 는 흡수하지 못했다. [^14C]glucose 흡수는 두 번째 성장기 세포에서 관찰할 수 있었다. 벼의 현탁배양 세포와 마찬가지로 당근의 현탁배양세포도 acetate를 일차적인 탄소원으로 사용하는 diauxic growth가 관찰되었다. We have been investigating acetate-glucose diauxic growth in plant. It was reported that rice (monocotyledon) suspension cells were exhibited diauxic growth on acetate (10 mM) and glucose (10 mM) medium (1). To investigate diauxic growth in dicotyledoneous plant, carrot (Daucus carota L.) suspension cells were grown on acetate and glucose medium Suspension culture of carrot exhibited diauxic growth when grown in a medium containing both acetate and glucose as carbon sources. Preferential utilization of acetate was observed in the initial growth phase of diauxic growth, whereas the second growth phase occured at the expense of glucose. Cells harvested during the first growth phase exhibited a capacity uptake of [^14C]acetate but not of [^14C]glucose. A capacity for [^14C]glucose uptake was expressed during the second growth phase. Like in rice suspension cells, in carrot suspension cells acetate was used as the primary carbon source during the acetate-glucose diauxic growth.
이화영,손희정,이규택,김재준,백승운,이종균,이준혁,고광철,이풍렬,이종철,손정일,서연림,전성국 대한소화기내시경학회 1997 Clinical Endoscopy Vol.17 No.1
In Korea, careinoma of the cervix is the most common malignancy in women annually affecting 25 per every 100,000 female. The widespread use of the Papanicolaou smear has resulted in earlier detection of the tumor and a reduction in the number of patients with advanced disease, but the incidence of cervix. carcinoma is still the fifth most common malignancy in the world. Cervical carcinoma spreads to the small bowel primarily by direct extension from involved lymph nodes, most commonly from peraaortic or mesenteric nodes to the serosa of the bowel. But, the involvement of small bowel by cervical carcinoma is so rare, and shows a maximum incidence of small bowel involvement of less than 1.5%. We report a patient with squamous cell carcinoma of the cervix metastases to the second portion of the duodenum in whom the diagnosis was made by endoscopic biopsy of the lesion.
Changes in gene expression profile due to acute toxicity of toxaphene in the marine medaka
Lee, Aekyung,Woo, Seonock,Won, Hyokyoung,Lee, Gunsup,Lee, Taek-Kyun,Yum, Seungshic 대한독성유전단백체학회 2013 Molecular & cellular toxicology Vol.9 No.2
Differential gene expression profiling was performed using cDNA microarray hybridization on the hepatic tissue of the marine medaka (Oryzias javanicus) after exposure to toxaphene, which is classified as a persistent organic pollutant. Ninety-seven differentially expressed candidate genes were identified; 40 were induced and 57 were repressed (P<0.05). The genes were assembled into 18 groups based mainly on the Eukaryotic Orthologous Groups classification. These isolated gene candidates were differentially expressed and therefore have great potential as molecular biomarkers for identifying environmental stressors and prognosis for the biological effects of the toxicant. Some of the genes were closely related to endocrine disruption, renal and cardiovascular disease, tumorigenesis, immune responses, and detoxification. Our results will allow future studies to assess the molecular mechanisms of toxaphene toxicity and to develop a systems biology approach to environmental stress biology.
Lee, Gunsup,Yu, Jaelim,Cho, Seungchan,Byun, Sung-June,Kim, Dae Hyun,Lee, Taek-Kyun,Kwon, Myung-Hee,Lee, Sukchan Public Library of Science 2014 PLoS pathogens Vol.10 No.6
<▼1><P>Viral protein neutralizing antibodies have been developed but they are limited only to the targeted virus and are often susceptible to antigenic drift. Here, we present an alternative strategy for creating virus-resistant cells and animals by ectopic expression of a nucleic acid hydrolyzing catalytic 3D8 single chain variable fragment (scFv), which has both DNase and RNase activities. HeLa cells (SCH7072) expressing 3D8 scFv acquired significant resistance to DNA viruses. Virus challenging with Herpes simplex virus (HSV) in 3D8 scFv transgenic cells and fluorescence resonance energy transfer (FRET) assay based on direct DNA cleavage analysis revealed that the induced resistance in HeLa cells was acquired by the nucleic acid hydrolyzing catalytic activity of 3D8 scFv. In addition, pseudorabies virus (PRV) infection in WT C57BL/6 mice was lethal, whereas transgenic mice (STG90) that expressed high levels of 3D8 scFv mRNA in liver, muscle, and brain showed a 56% survival rate 5 days after PRV intramuscular infection. The antiviral effects against DNA viruses conferred by 3D8 scFv expression in HeLa cells as well as an <I>in vivo</I> mouse system can be attributed to the nuclease activity that inhibits viral genome DNA replication in the nucleus and/or viral mRNA translation in the cytoplasm. Our results demonstrate that the nucleic-acid hydrolyzing activity of 3D8 scFv confers viral resistance to DNA viruses <I>in vitro</I> in HeLa cells and in an <I>in vivo</I> mouse system.</P></▼1><▼2><P><B>Author Summary</B></P><P>Most strategies for developing virus-resistant transgenic cells and animals are based on the concept of virus-derived resistance, in which dysfunctional virus-derived products are expressed to interfere with the pathogenic process of the virus in transgenic cells or animals. However, these viral protein targeting approaches are limited because they only target specific viruses and are susceptible to viral mutations. We describe a novel strategy that targets the viral genome itself, rather than viral gene products, to generate virus-resistant transgenic cells and animals. We functionally expressed 3D8 scFv which has both DNase and RNase activities, in HeLa cells and transgenic mice. We found that the transgenic cells and mice acquired complete resistance to two DNA viruses (HSV and PRV) without accumulating the virus, and showed delayed onset of disease symptoms. The antiviral effects against DNA viruses demonstrated in this study were caused by (1) DNase activity of 3D8 scFv in the nucleus, which inhibited DNA replication or RNA transcription and (2) 3D8 scFv RNase activity in the cytoplasm, which blocked protein translation. This strategy may facilitate control of a broad spectrum of viruses, including viruses uncharacterized at the molecular level, regardless of their genome type or variations in gene products.</P></▼2>
Lee, Jung-Sup,Park, Yong-Sook,Kwon, Jeong-Taik,Nam, Taek-Kyun,Lee, Tae-Jin,Kim, Jae-Kyun The Korean Neurosurgical Society 2011 Journal of Korean neurosurgical society Vol.50 No.4
Objective : Pituitary apoplexy is life-threatening clinical syndrome caused by the rapid enlargement of a pituitary tumor due to hemorrhage and/or infarction. The pathogenesis of pituitary apoplexy is not completely understood. We analyzed the magnetic resonance imaging (MRI) of pituitary tumors and subsequently correlated the radiological findings with the clinical presentation. Additionally, immunohistochemistry was also performed to determine whether certain biomarkers are related to radiological apoplexy. Methods : Thirty-four cases of pituitary adenoma were enrolled for retrospective analysis. In this study, the radiological apoplexy was defined as cases where hemorrhage, infarction or cysts were identified on MRI. Acute clinical presentation was defined as the presence of any of the following symptoms: severe sudden onset headache, decreased visual acuity and/or visual field deficit, and acute mental status changes. Angiogenesis was quantified by immunohistochemical expression of fetal liver kinase 1 (Flk-1), neuropilin (NRP) and vascular endothelial growth factor (VEGF) expression, while microvascular density (MVD) was assessed using Endoglin and CD31. Results : Clinically, fourteen patients presented with acute symptoms and 20 for mild or none clinical symptoms. Radiologically, fifteen patients met the criteria for radiological apoplexy. Of the fifteen patients with radiologic apoplexy, 9 patients presented acute symptoms whereas of the 19 patient without radiologic apoplexy, 5 patients presented acute symptoms. Of the five biomarkers tracked, only VEGF was found to be positively correlated with both radiological and nonradiological apoplexy. Conclusion : While pituitary apoplexy is currently defined in cases where clinical symptoms can be histologically confirmed, we contend that cases of radiologically identified pituitary hemorrhages that present with mild or no symptoms should be designated subacute or subclinical apoplexy. VEGF is believed to have a positive correlation with pituitary hemorrhage. Considering the high rate of symptomatic or asymptomatic pituitary tumor hemorrhage, additional studies are needed to detect predictors of the pituitary hemorrhage.