퍼지 제어기 소속함수의 추정을 위한 진화프로그래밍 이용에 관한 연구

김승철,장종승,강신출,임영도 東亞大學校 附設 情報通信硏究所 1996 情報通信硏究所論文誌 Vol.4 No.1

On this paper, the design of fuzzy adaptive controller using an evolution programming combining fuzzy controller to be very effectove in the optimization problem will be proposed. On a proposing control structure, the optimization takes uncertain plants optimized membership function using EP, and we want to take an adaptive controller in the plant as well be adapted control structure to initiate approximate membership function in fuzzy. We show that proposed controller have a good performance than general fuzzy controller through a computer simulation and the real-time control of DC serve motor.

레이저에 의한 表面 局所 加熱 時 黑體 塗料가 表面 溫度 變化에 주는 影響

金鍾喆,申澈洙,姜宇政,朴壽泉 충주대 산업과학기술연구소 1996 産業科學論文集 Vol.4 No.-

Measuring the thermophysical properties of solids in laser heating method, the material surface is coated by thin black paint layer(graphite) to minimize the effects of the laser beam reflection. But, the influence of the thin black paint layer on the surface temperature variations have been ignored. In this paper, a numerical analysis is carried out to clarify quantitatively the influence of the thin black paint layer when the material surface is heated locally. In the experiment, the material surface is heated by Ar laser beam and the surface temperature profiles are measured by an infrared thermometer.

인듀서 내부의 캐비테이션 유동과 흡입성능에 관한 연구

申澈洙,金鍾喆,姜宇政,李先基 충주대 산업과학기술연구소 1995 産業科學論文集 Vol.3 No.-

In order to understand the influence of the inducer inlet angle on cavitation and suction performance, experiments were carried out for two kinds inducer, which have the same outlet angle distribution, length and number of inducer blades. The results showed that the change of inducer inlet angles influenced not only internal flow conditions but also cavity flow and suction performance. The improvement of suction performance by using inducer were also discussed.

전원지역 초등학교계속구강건강관리 사업 모형의 개발에 관한 연구

윤신종,박기철,신승철,김광영 단국대학교 치과대학 예방치과 1998 예방치과 세미나집 Vol.- No.12

The purpose of this study was to develop of a model for School based Oral health program in Korea. Study model of school based dental program was established at Mockcheon Grade school, in 1989 and followed the program for 6 years. 324 school children have been participated in the program. Dental students and dental hygienist's students of Dankook University School of Dentistry have been providing al dental services under the supervision of a public health dentist. Primary preventive care such as sealants, fluoride mouthrinse fluoride topical applications, toothbrusing instruction, oral prophylaxis, were provided to all of the participendents by the students under the supervision of public health dentists. Data for DMF rate, DMFT rate and DMFT index were collected annually. The amounts of money and time consumptions for this program were also monitored. The results obtained for this study were as followings ; 1. The combined school based oral health care program utilized in Mockcheon primary school resulted a reduction of 33.5% in caries incidence after 6 years of implemention. 2. The school based oral health care program tested in this program reduced an average of 53% of dental treatment cost for school children ofter 6 years of implemention. 3. It is therefore suggested that the school based oral health care program is an effective means of public health project for school children in Korea.

Induction of Endogenous Neural Stem Cells By Extracorporeal Shock Waves After Spinal Cord Injury

Shin, Dong-Cheul,Ha, Kee-Yong,Kim, Young-Hoon,Kim, Jang-Woon,Cho, Yoo-Kyung,Kim, Sang-Il Lippincott Williams & Wilkins 2018 Spine Vol.43 No.4

STUDY DESIGN.: Animal experimental study OBJECTIVES.: The purpose of this study is to investigate the effects of extracorporeal shock waves (ESWs) on endogenous neural stem cells (NSCs) proliferation after spinal cord injury (SCI). SUMMARY OF BACKGROUND DATA.: Exogenous stem cell transplantation for SCI still has many limitations to be addressed such as ideal cell sources, timing of transplantation, and fate of the transplanted cells. Moreover, the efficacy is another issue owing to a peculiar pathologic condition in the chronic phase of SCI. METHODS.: Contusive SCI was made using 24 Sprague-Dawley rats, and ESWs were applied at post-injury 4 weeks in rats. Proliferation and differentiation of endogenous NSCs (DCX, Sox-2) and axonal sprouting (GAP-43 and MAP-2) were observed at 6 weeks after application of ESWs. Differentiation of the activated neural stem cells was also investigated by coexpression of neuronal/glial cell markers (GFAP, Neu N, and CC-1). Immunofluorescence staining and western blotting were performed for quantitative analysis, and these results were compared with those in the control group. For clinical assessment, the BBB locomotor rating scale was performed. RESULTS.: More proliferation of endogenous neural stem cells was noted in the experimental groups, and these activated cells were mainly founded in the ependymal layer of the central canal and the injured posterior horn. Differentiation into neuronal and glial cells was also noted in a limited number of cells. With respect to axonal regeneration, GAP-43 and MAP-2 expressions in the experimental groups were also significantly higher than those in the control group. During 6 weeks’ clinical observation following ESWs application, functional improvement of the hindlimb was observed without clinical deterioration by trials. CONCLUSION.: Collectively, these findings indicate that ESWs on the chronic phase of SCI induce activation of endogenous NSCs and consequent functional improvement.Level of Evidence: N/A

아데노 바이러스를 이용한 Telomerase-Antisense 발현이 조혈모세포 체외 배양에 미치는 영향

김석진,김병수,오상철,서재홍,최철원,신상원,김열홍,김준석,김영태,송준석,최은정 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.1

연구배경: 조혈모세포는 자가갱신능 및 분화능을 지니고 있지만, 조혈모세포의 체외배양을 통한 자가갱신을 유도하는 기술 및 정상 혈액세포로 분화, 증식시키는 기술이 개발되어 있지 않고 있다. 그런데, 줄기세포의 자가갱신에 telomerase가 매우 중요한 역할을 수행하고 있음이 알려져 있고 이에 저자 등은 조혈모세포를 telomerase antisense로 처리 후 체외 배양을 하였을 때 어떤 결과가 초래되는지를 알아보고자 본 연구를 시행하였다. 방법: 총 8명의 정상인 골수 공여자에서 채집한 골수혈로부터 단핵구를 분리 후 1×10^(5)/ml의 농도로 LTC-IC 배양을 한 대조군과 단핵구(1×10^(5)/ml)를 telomerase antisense (Adenovirus, 10⁴ PFU/ml, 3 ml) 처리 후 LTC-IC 배양을 한 실험군으로 나누어 양군간의 배양 2주 후 세포 수, 생존율, 그리고 세포모양을 비교, 분석하였다. 결과: LTC-IC 배양 2주에 계산한 세포 수의 경우, 대조군은 1.08±0.19×10⁴/ml인 데 반하여 실험군은 6.75±2.60×10⁴/ml로 통계적으로 유의하게 실험군에서 세포 수가 많았다(P=0.001). LTC-IC 배양 2주에 관찰한 세포 모양의 경우 대조군은 모든 세포들이 일정한 크기로 구형을 유지하고 있었으나 실험군에서는 크기가 서로 상이하고 분화된 세포들이 상당 부분에서 관찰되었다. Tryphan dye로 염색 후 100개의 세포를 관찰하여 염색되지 않은 세포를 생존 세포로 간주하여 그 백분율을 계산하여 보았다. 그 결과 대조군은 83.8±4.8%이고 실험군은 81.1±2.1%로서 통계적으로 유의한 차이는 없었다(P=0.288). 결론: 조혈 모세포를 Telomerase Antisense로 처리 시 분화가 촉진될 가능성을 본 실험을 통하여 확인할 수 있었다. 그러나 본 실험에서는 Telomerase Antisense로 처리 후 LTC-IC 배양으로 증식된 세포들의 성상 하나, 하나를 확인하지 못하였다는 한계가 있었다. Background: Hematopoieic stem cells are characterized by their ability of self-renewal and differentiation into mature blood cells. Telomerase, which is known as a target of anti-cancer therapy, is also regarded as having possibility of inducing self-renewal of hematopoietic stem cells, and inhibition of telomerase activity might be related with differentiation of hematopoietc stem cells. Thus, we evaluated how telomerase antisense oligonucleotide could affect hematopoietic stem cells in vitro culture. Methods: We used adenovirus vector containing recombinant antisense telomerase template RNA. Mononuclear cells were obtained from 8 bone marrow donors and divided into two groups. Using the long-term culture system, the experiment group was cultured with treatment of telomerase antisense oligonucleotides, while control group was not. We evaluated the cell number and the degree of differentiation at 2 weeks after LTC-IC culture. Results: The mean cell number of LTC-IC culture was more higher in the telomerase antisense treatment group than that of control group (6.75×10⁴±2.60/mL vs 1.08×10⁴±0.19/mL). On microscopic examination, the cells of telomerase antisense treatment group showed more differentiation pattern than the group of the control group. Conclusion: We found that adenovirus-mediated antisense expression of telomerase RNA might influence the differentiation of hematopoietic stem cells. However, further study should be warranted to evaluate its consequences and mechanisms.

Copper-Vapor-Assisted Chemical Vapor Deposition for High-Quality and Metal-Free Single-Layer Graphene on Amorphous SiO₂ Substrate

Kim, Hyungki,Song, Intek,Park, Chibeom,Son, Minhyeok,Hong, Misun,Kim, Youngwook,Kim, Jun Sung,Shin, Hyun-Joon,Baik, Jaeyoon,Choi, Hee Cheul American Chemical Society 2013 ACS NANO Vol.7 No.8

<P>We report that high-quality single-layer graphene (SLG) has been successfully synthesized directly on various dielectric substrates including amorphous SiO<SUB>2</SUB>/Si by a Cu-vapor-assisted chemical vapor deposition (CVD) process. The Cu vapors produced by the sublimation of Cu foil that is suspended above target substrates without physical contact catalyze the pyrolysis of methane gas and assist nucleation of graphene on the substrates. Raman spectra and mapping images reveal that the graphene formed on a SiO<SUB>2</SUB>/Si substrate is almost defect-free and homogeneous single layer. The overall quality of graphene grown by Cu-vapor-assisted CVD is comparable to that of the graphene grown by regular metal-catalyzed CVD on a Cu foil. While Cu vapor induces the nucleation and growth of SLG on an amorphous substrate, the resulting SLG is confirmed to be Cu-free by synchrotron X-ray photoelectron spectroscopy. The SLG grown by Cu-vapor-assisted CVD is fabricated into field effect transistor devices without transfer steps that are generally required when SLG is grown by regular CVD process on metal catalyst substrates. This method has overcome two important hurdles previously present when the catalyst-free CVD process is used for the growth of SLG on fused quartz and hexagonal boron nitride substrates, that is, high degree of structural defects and limited size of resulting graphene, respectively.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2013/ancac3.2013.7.issue-8/nn402847w/production/images/medium/nn-2013-02847w_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn402847w'>ACS Electronic Supporting Info</A></P>

Propofol promotes osteoclastic bone resorption by increasing DC-STAMP expression

Kim, Eun-Jung,Kim, Hyung Joon,Baik, Seong Wan,Kim, Kyung-Hoon,Ryu, Sie Jeong,Kim, Cheul-Hong,Shin, Sang-Wook The Korean Dental Society of Anesthsiology 2018 Journal of Dental Anesthesia and Pain Medicine Vol.18 No.6

Background: Propofol is an intravenous anesthetic which has antioxidant effects due to its similarity in molecular structure to ${\alpha}$-tocopherol. It has been reported that ${\alpha}$-tocopherol increases osteoclast fusion and bone resorption. Here, we investigated the effects of propofol on signaling pathways of osteoclastogenic gene expression, as well as osteoclastogenesis and bone resorption using bone marrow-derived macrophages (BMMs). Methods: BMMs were cultured with macrophage colony-stimulating factor (M-CSF) alone or M-CSF plus receptor activator of nuclear factor kappa B ligand (RANKL) in the presence of propofol ($0-50{\mu}M$) for 4 days. Mature osteoclasts were stained for tartrate-resistant acid phosphatase (TRAP) and the numbers of TRAP-positive multinucleated osteoclasts were counted. To examine the resorption activities of osteoclasts, a bone resorption assay was performed. To identify the mechanism of action of propofol on the formation of multinucleated osteoclasts, we focused on dendritic cell-specific transmembrane protein (DC-STAMP), a protein essential for pre-osteoclastic cell fusion. Results: Propofol increased the formation of TRAP-positive multinucleated osteoclasts. In addition, the bone resorption assay revealed that propofol increased the bone resorption area on dentin discs. The mRNA expression of DC-STAMP was upregulated most strongly in the presence of both RANKL and propofol. However, SB203580, a p38 inhibitor, significantly suppressed the propofol/RANKL-induced increase in mRNA expression of DC-STAMP. Conclusion: We have demonstrated that propofol enhances osteoclast differentiation and maturation, and subsequently increases bone resorption. Additionally, we identified the regulatory pathway underlying osteoclast cell-cell fusion, which was enhanced by propofol through p38-mediated DC-STAMP expression.

Voltage-gated calcium channels play crucial roles in the glutamate-induced phase shifts of the rat suprachiasmatic circadian clock

Kim, Do Young,Choi, Hee Joo,Kim, Jeong Sook,Kim, Yoon Sik,Jeong, Do Ung,Shin, Hyung Cheul,Kim, Mi Jin,Han, Hee-Chul,Hong, Seung Kil,Kim, Yang In Blackwell Science Ltd 2005 The European journal of neuroscience Vol.21 No.5

<P>Abstract</P><P>The resetting of the circadian clock based on photic cues delivered by the glutamatergic retinohypothalamic tract is an important process helping mammals to function adaptively to the daily light–dark cycle. To see if the photic resetting relies on voltage-gated Ca<SUP>2+</SUP> channels (VGCCs), we examined the effects of VGCC blockers on the glutamate-induced phase shifts of circadian firing activity rhythms of suprachiasmatic nucleus (SCN) neurons in hypothalamic slices. First, we found that a cocktail of amiloride, nimodipine and &ohgr;-conotoxin MVIIC (T-, L- and NPQ-type VGCC antagonists, respectively) completely blocked both phase delays and advances, which were, respectively, induced by glutamate application in early and late night. Next, we discovered that: (i) amiloride and another T-type VGCC antagonist, mibefradil, completely obstructed the delays without affecting the advances; (ii) nimodipine completely blocked the advances while having less impact on delays; and (iii) &ohgr;-conotoxin MVIIC blocked largely, if not entirely, both delays and advances. Subsequent whole-cell recordings revealed that T-type Ca<SUP>2+</SUP> currents in neurons in the ventrolateral, not dorsomedial, region of the SCN were larger during early than late night, whereas L-type Ca<SUP>2+</SUP> currents did not differ from early to late night in both regions. These results indicate that VGCCs play important roles in glutamate-induced phase shifts, T-type being more important for phase delays and <SMALL>L</SMALL>-type being so for phase advances. Moreover, the results point to the possibility that a nocturnal modulation of T-type Ca<SUP>2+</SUP> current in retinorecipient neurons is related to the differential involvement of T-type VGCC in phase delays and advances.</P>

Characteristics of AlN thin film prepared by biased reactive RF mangetron sputtering

Shin Cheul Kim,Yong Suk Roh,Ui Sik Kim,Kwangho Jeong 한국진공학회 1998 한국진공학회 학술발표회초록집 Vol.- No.-