Three New 29 Carbon Skeletons Pentacyclic Triterpenoids and S-equol from Biogas Slurry

Jian-Feng Xu,Hui-Bin Wu,Ding-Cai Liu,Long Sha,Wen-Hui Wu,Hua Fan,Yishan Song,Hong-Guang Zhu 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.12

Bioactive natural products were firstly obtained from biogas slurry. Three new 29 carbon skeletons of the pentacyclic triterpenoid compounds 24-norolean-12-ene-3,22-dione (1), 3β-hydroxy-24-norolean-12-ene-22-one (2), 3α-hydroxy-24-norolean-12-ene-22-one (3), as well as one known compound S-equol (4) were isolated and purified from the MeOH extract of chicken manure biogas slurry. The molecular structures of the four compounds were elucidated based on the extensive spectroscopic data analysis, and the structure of Compound 1 was further confirmed by single-crystal X-ray diffraction. The structures of Compounds 1, 2, and 3 are similar with oleanolicum and hederagenin that has excellent anti-tumor activities. The cytotoxicity against five cancer cell lines (Hela, A549, MCF7, PC3, and B16) of Compounds 1–4 was tested. Similar to Compound 4 (S-equol), Compounds 1–3 (pentacyclic triterpenoids) showed cytotoxicity activity against different tumor cell lines. Compounds 1–3 showed slightly lower cytotoxicity activities than Compound 4. The IC50 of Compound 4 was determined to be 9.7–27.6 μM, while the IC50 values of 1–3 were 17.6–65.3 μM. There are no significant differences in the cytotoxicity capacities between Compounds 1, 2, and 3.

In vitro evaluation of 9-(2-phosphonylmethoxyethyl)adenine ester analogues, a series of anti-HBV structures with improved plasma stability and liver release

Sha Liao,Shi-Yong Fan,Qin Liu,Chang-Kun L,Jia Chen,Jing-Lai Li,Zhi-Wei Zhang,Zhen-Qing Zhang,Bo-Hua Zhong,Jian-Wei Xie 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.11

Chronic hepatitis B virus (HBV) infection maylead to liver cirrhosis and hepatocellular carcinoma, butfew drugs are available for its treatment. Acyclic nucleosidephosphonates (ANPs) have remarkable antivirusactivities but are not easily absorbed from the gastrointestinaltract and accumulate in the kidneys, resulting innephrotoxicity. Therefore, there is a need to find effectiveliver site-specific prodrugs. The dipivaloyloxymethyl esterof 9-(2-phosphonylmethoxyethyl)adenine (PMEA)—adefovirdipivoxil (ADV)—is a first-line therapy drug forchronic hepatitis B with a low therapeutic index because ofrenal toxicity and low hepatic uptake. In this study, a seriesof PMEA derivatives were synthesized to enhance plasmastability and liver release. The metabolic stability of ADV(Chemical I) and its two analogues (Chemicals II and III)was evaluated in rat plasma and liver homogenate in vitro. An ion-pair reverse-phase HPLC–UV method and a hybridion trap and high-resolution time-of-flight mass spectrometry(LC-IT-TOF-MS) were used to evaluate the degradationrate of the analogues and to identify their intermediatemetabolites, respectively. Chemicals I and II were hydrolyzedby cleavage of the C–O bond to give monoesters. Sufficient enzymatic activation in the liver homogenatethrough a relatively simple metabolic pathway, in additionto a favorable stability profile in rat plasma, made ChemicalII an optimal candidate. Next, six analogues based onthe structure of Chemical II were synthesized and evaluatedin plasma and liver homogenate. Compared toChemical II, these compounds generated less active PMEAlevels in rat liver homogenate. Therefore, chemical modificationof Chemical II may lead to new promising PMEAderivatives with enhanced plasma stability and liveractivation.

Molecular and cytological evidences for the natural wheatgrass hybrids occurrence and origin in west China

Jian Zeng,Xing Fan,Hai-Qin Zhang,Li-Na Sha,Hou-Yang Kang,Li Zhang,Rui-Wu Yang,Chun-Bang Ding,Yong-Hong Zhou 한국유전학회 2012 Genes & Genomics Vol.34 No.5

Interspecies hybridization has been frequently observed in the tribe Triticeae. Natural hybridization between Kengyilia and Roegneria or Elymus species has not been reported as yet. Several sterile wheatgrass individuals exhibiting intermediately morphological traits between Kengyilia and Roegneria or Elymus species were identified in the meadow of Sichuan and Gansu provinces in China, suggesting their natural hybrid origin. The putative hybrids were analyzed by using the sequences of ITS and trnH-psbA together with cytological observation in order to assess the origin of hybrids. Both ITS and cytological data revealed the evidence of allopolyploid origin and confirmed the presence of StStYYP and StStYYHP genomes in the putative natural hybrids. The data suggest that the StStYYP hybrid originated from hybridization between Kengyilia and Roegneria and the hybrid with StStYYHP originated from hybridization between Kengyilia and Elymus. Chloroplast sequence data demonstrated that K. rigidula and K. melanthera were the likely maternal donors in the hybridization events.

Structure-based functional site recognition for p21-activated kinase 4

Jian Wang,Gang Wang,Yu Sha,Dong-Mei Zhao,Feng Li,Mao-Sheng Cheng 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.12

Recently, many molecular modeling methodsare being developed to better understand the principlesunderlying protein folding. In the present study, fullyflexible dinucleotides d(pApA), d(pApC), d(pApG),d(pApT), d(pCpA), d(pCpC), d(pCpG), d(pCpT), d(pGpA),d(pGpC), d(pGpG), d(pGpT), d(pTpA), d(pTpC), d(pTpG)and d(pTpT) were docked onto the surface of p21-activatedkinase 4 (PAK4) kinase domain. The results showedthat automated docking was a useful tool to identify thefunctional sites of PAK4 and it may provide a theoreticalbasis for the interaction data obtained from previousexperiments. Therefore, structure-based docking with fullyflexible dinucleotide probes might be a good tool to predictand annotate the functional sites of enzymes.

Proline Enhances Torulopsis glabrata Growth during Hyperosmotic Stress

Sha Xu,Jingwen Zhou,Liming Liu,Jian Chen 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.2

This study investigated that the importing of compatible solute proline could enhance the growth of the yeast Torulopsis glabrata under hyperosmotic stress. Osmolarity progressively increased from 860 to 2,603mOsmol/kg by accumulation of sodium pyruvate in the culture broth, leading to a significant decrease in cell growth. When 1.0 g/L of proline as a compatible solute was added to the culture medium, it was imported and enhanced cell growth by 59.0% at 2,603 mOsmol/kg. By addition of proline during pyruvate production, the concentration,productivity, and yield of pyruvate increased 22.1, 38.4, and 14.3%, respectively. These results suggested that T. glabrata can import proline as an osmoprotectant against high osmotic stress, thus enhance pyruvate productivity. The improvement of yeast growth and viability under hyperosmotic stress by the addition of proline provided an alternative approach to enhance the organic acids production by yeast.

Association of Triglyceride Glucose-Derived Indices with Recurrent Events Following Atherosclerotic Cardiovascular Disease

Sha Li,Hui-Hui Liu,Yan Zhang,Meng Zhang,Hui-Wen Zhang,Cheng-Gang Zhu,Yuan-Lin Guo,Na-Qiong Wu,Rui-Xia Xu,Qian Dong,Ke-Fei Dou,Jie Qian,Jian-Jun Li 대한비만학회 2024 Journal of obesity & metabolic syndrome Vol.33 No.2

Background: Triglyceride glucose (TyG) and TyG-body mass index (TyG-BMI) are reliable surrogate indices of insulin resistance and used for risk stratification and outcome prediction in patients with atherosclerotic cardiovascular disease (ASCVD). Here, we inserted estimated average glucose (eAG) into the TyG (TyAG) and TyG-BMI (TyAG-BMI) as derived parameters and explored their clinical significance in cardiovascular risk prediction. Methods: This was a population-based cohort study of 9,944 Chinese patients with ASCVD. The baseline admission fasting glucose and A1C-derived eAG values were recorded. Cardiovascular events (CVEs) that occurred during an average of 38.5 months of follow-up were recorded. We stratified the patients into four groups by quartiles of the parameters. Baseline data and outcomes were analyzed. Results: Distribution of the TyAG and TyAG-BMI indices shifted slightly toward higher values (the right side) compared with TyG and TyG-BMI, respectively. The baseline levels of cardiovascular risk factors and coronary severity increased with quartile of TyG, TyAG, TyG-BMI, and TyAG-BMI (all P<0.001). The multivariate-adjusted hazard ratios for CVEs when the highest and lowest quartiles were compared from low to high were 1.02 (95% confidence interval [CI], 0.77 to 1.36; TyG), 1.29 (95% CI, 0.97 to 1.73; TyAG), 1.59 (95% CI, 1.01 to 2.58; TyG-BMI), and 1.91 (95% CI, 1.16 to 3.15; TyAG-BMI). The latter two showed statistical significance. Conclusion: This study suggests that TyAG and TyAG-BMI exhibit more information than TyG and TyG-BMI in disease progression among patients with ASCVD. The TyAG-BMI index provided better predictive performance for CVEs than other parameters.

Impact of Lymphadenectomy for Patients with Clinically Node-Negative Intrahepatic Cholangiocarcinoma: A Multicenter Retrospective Cohort Study

( Meng Sha ),( Jie Cao ),( Cheng-lin Qin ),( Jian Zhang ),( Zhe Li ),( De-fu Kong ),( Hua-lian Hang ),( Ying Tong ),( Qiang Xia ) 대한간학회 2024 춘·추계 학술대회 (The Liver Week) Vol.2024 No.1

Numerical Analysis and Load-Carrying Capacity Estimation of Reinforced Concrete Slab Culvert Rehabilitated with a Grouted Corrugated Steel Plate

Li Bai-Jian,Fu Wen-Qiang,Fu Xin-Sha,Huang Yan 한국강구조학회 2023 International Journal of Steel Structures Vol.23 No.4

Two laboratory tests were conducted to investigate the mechanical properties and develop a load-carrying capacity estimation method for reinforced concrete (RC) slab culverts rehabilitated with a grouted corrugated steel plate (CSP). Subsequently, 216 numerical models of RC slab culverts rehabilitated with different shapes of CSPs and grout strengths were established to investigate the influence of these parameters and the arch effect on the rehabilitated system. A mechanical model was proposed based on the elastic center method, and a load-carrying capacity estimation method of RC slab culverts rehabilitated with grouted CSPs was established and verified. It was concluded that the load-carrying capacity of the rehabilitated system increased with a decrease in the radius of the side walls and crown at a constant radius of the CSP haunch. At a constant radius of the side walls and CSP crown, the load-carrying capacity of the rehabilitated system increased with an increase in the haunch radius. The most effective way to improve the load-carrying capacity of the rehabilitated system was to increase the radius of the haunches and reduce the radius of the arch crown and side wall. The arch effect of the grout was related to the load type. The load-carrying capacity of the rehabilitated system was the highest when the CSP was similar to or the same as the arch axes of the grout. The most important function of the grout was to provide strong lateral restraint for the CSP, reducing the required span and improving the load-carrying capacity of the CSP. In addition, the shear strength of the grout contributed to improving the load-carrying capacity by exerting an arch effect or experiencing shear failure. The proposed load-carrying capacity estimation method is applicable to rehabilitated systems with a box or arch-type CSP. Our findings provide guidance for engineers to design similar rehabilitated systems.

Genomic Sequence Analysis and Organization of BmKαTx11 and BmKαTx15 from Buthus martensii Karsch: Molecular Evolution of α-toxin genes

Xiu Ling Xu,Zhi Jian Cao,Ji Qun Sheng,Wen Lan Wu,Feng Luo,Yong Gang Sha,Xin Mao,Hui Liu,Da He Jiang,Wen Xin Li 한국생활환경학회 2005 BMB Reports Vol.38 No.4

Hypersonic Aero-Heating Ground-Test Simulation Technique

Li, Ruiqu,Yao, Dapeng,Sha, Xinguo,Gong, Jian The Society for Aerospace System Engineering 2015 International Journal of Aerospace System Engineer Vol.2 No.2

It would encounter some complicated flow fields, such as transition, separation, reattachment and disturbances, in the hypersonic flight. Thus, it is difficult to theoretically analyze the hypersonic aerothermodynamics effects, so that the ground-test simulation is thought of as one of the most important methods to improve the understanding level of the hypersonic aerothermodynamics. However, the aero-heating tests could not simulate all aerodynamics and geometry parameters in the real flight due to the differences between the experimental environments supplied by the ground facilities and the flight, so that the feasible technique for the ground-test simulation of the hypersonic aerothermodynamics effects is required to be advanced. The key parameters that are especially required to simulate for aero-heating tests are analyzed and one detailed approach is suggested to perform the experimental investigation on the hypersonic aero-heating effects in the ground facilities in this paper, and the tests are performed in the FD-20 gun tunnel of CAAA (China Academy of Aerospace Aerodynamics) to give out the data which could be used to confirm the equation from the theoretical analysis.