Effect of Non-animal-Derived Nitrogen Sources on the Production of Hyaluronic Acid by Streptococcus sp. KL0188

Lee, Gil-Yong,Ha, Suk-Jin,Jung, Jong-Hyun,Seo, Dong-Ho,Park, Jong-Yul,Kim, Su-Rin,Park, Nam-Woo,Kweon, Dong-Keon,Park, Sang-Hoo,Park, Cheon-Seok The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.3

Hyaluronic acid (HA) is a linear high-molecular-weight polysaccharide with useful biomedical applications. Streptococcus zooepidemicus, a typical HA-producing bacterium, requires an animal-derived nitrogen source such as tryptone, peptone or sheep blood as a nutrient. Sixteen non-animal-derived (NAD) nitrogen sources were tested as a replacement for the expensive animal-derived nitrogen sources, which may have safety issues. Among the sixteen tested NAD nitrogen sources, a yeast-derived nitrogen source (YE 0251) showed the highest HA productivity, which was equivalent to the control HA production medium containing tryptone in a 5-L jar and in 3,000-L industrial fermentations. In the 3,000-L fermentation, YE 0251 increased cell mass (dry cell weight) and HA production by 11% and 8%, respectively, compared with the control HA production medium. The fmal specific volumetric productivity (0.41 g/L h) was improved by about 70% after reducing the fermentation time from 20 h to 12 h, compared to the conventional production medium.

Bloodstream Infections and Clinical Significance of Healthcare-associated Bacteremia: A Multicenter Surveillance Study in Korean Hospitals

Son, Jun Seong,Song, Jae-Hoon,Ko, Kwan Soo,Yeom, Joon Sup,Ki, Hyun Kyun,Kim, Shin-Woo,Chang, Hyun-Ha,Ryu, Seong Yeol,Kim, Yeon-Sook,Jung, Sook-In,Shin, Sang Yop,Oh, Hee Bok,Lee, Yeong Seon,Chung, Doo The Korean Academy of Medical Sciences 2010 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.25 No.7

<P>Recent changes in healthcare systems have changed the epidemiologic paradigms in many infectious fields including bloodstream infection (BSI). We compared clinical characteristics of community-acquired (CA), hospital-acquired (HA), and healthcare-associated (HCA) BSI. We performed a prospective nationwide multicenter surveillance study from 9 university hospitals in Korea. Total 1,605 blood isolates were collected from 2006 to 2007, and 1,144 isolates were considered true pathogens. HA-BSI accounted for 48.8%, CA-BSI for 33.2%, and HCA-BSI for 18.0%. HA-BSI and HCA-BSI were more likely to have severe comorbidities. <I>Escherichia coli</I> was the most common isolate in CA-BSI (47.1%) and HCA-BSI (27.2%). In contrast, <I>Staphylococcus aureus</I> (15.2%), coagulase-negative <I>Staphylococcus</I> (15.1%) were the common isolates in HA-BSI. The rate of appropriate empiric antimicrobial therapy was the highest in CA-BSI (89.0%) followed by HCA-BSI (76.4%), and HA-BSI (75.0%). The 30-day mortality rate was the highest in HA-BSI (23.0%) followed by HCA-BSI (18.4%), and CA-BSI (10.2%). High Pitt score and inappropriate empirical antibiotic therapy were the independent risk factors for mortality by multivariate analysis. In conclusion, the present data suggest that clinical features, outcome, and microbiologic features of causative pathogens vary by origin of BSI. Especially, HCA-BSI shows unique clinical characteristics, which should be considered a distinct category for more appropriate antibiotic treatment.</P>

담도에 국한되어 증상을 유발한 석회쓸개즙 2 예

윤성우,이지영,하경호,이수경,한나,배효근,박선자 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Limy bile is a rare condition characterized by excessive precipitation of calcium carbonate in the gallbladder or bile duct. Recently we experienced two cases with obstructive jaundice in which limy bile in the common bile duct but not in the gallbladder for one case, and in the common bile duct and intra hepatic duct but not in the gallbladder for another case. The patients were treated by endoscopic sphincterotomy. Here, we report the cases and review the literature.

피로하중에 의한 NITINOL의 기계적 성질의 변화

하국봉,손우성 대한치과교정학회 1993 대한치과교정학회지 Vol.23 No.4

Nitinol wires are now widely used in the orthodontic field because of their unique shape memory effect and superelasticity. But sometimes Nitinol wires are deformed in clinical use. Fatigue load is possible cause of Nitinol deformation. To determine the effect of fatigue load to the mechanical properties of Nitinol, various fatigue cycle(1×10^4, 2×10^4, 3×10^4, 5×10^4, 1×10^5) were applied to 0.017×0.025 inch Nitinol. The results obtained were as follows ; 1. Applied load increased as fatigue cycle increased in three point bending test. 2. Maximum tensile strength and elongation decreased as fatigue cycle increased. 3. In SEM,brittle fracture pattern was increased when fatigue cycle increased.

관광호텔 勞使關係의 安定化 방안에 관한 연구

하종명,이우상 진주여자전문대학 1997 論文集 Vol.20 No.-

The purposes of this study are to find out various significant factors impinging on the stability of labor-relations and their potential effects, and to propose effective approaches for enhancing the stability of labor-relations in tourist hotels. Specifically, the management system of tourist hotels and the empliyees' characteristics were selected as independent variables, and the stability of labor-relations was employed as subordinate variables. For these purposes, hypothesis was established as follows: Relation between independent variables(employees' characteristics) and the subor-dinate variable of the stability of labor-relations(labor harmony) should show significant difference. The samples of this study were 57 hotels in Seoul, Pusan, Taigu, TaiJun, Kyeongju, and Kyeongnam province. Enhancement of employees' Job attitude: the higher efficacy and pride the employees have toward their hotels, the higher degree of committment they may show to their Jobs, thus leading to lower rate of turnover and higher level of Job satisfaction among employees. Optimal labor-harmony may be secured when the employees are willing to sacrifice for the growth of their hotels. As a prerequisite for this harmony, new knowledge, skill, information which are required for employees' job performances must be provided to guarantee efficient communication.

한글 문서 구조 분석에 관한 연구

우성준,김광호,유명춘,하진영 강원대학교 정보통신연구소 2008 정보통신논문지 Vol.12 No.-

Although information technology has been developed fast, large amount of machine printed document images are not properly handled due to immature document layout analysis techniques. Previous research has focused on character recognition itself to improve character recognition accuracy. Recently, however, some efforts are made to analyze complex document layout automatically. In this paper, we propose document layout analysis and understanding method for various Hangul document images. Skew detection is performed using projection profile and connected component layout information. Top-down and bottom-up methods are combined to analyze document layout. After text region and non-text region determination, table is further processed to extract row, column, and cell position. We got very high performance for skew correction, zone detection, and table processig from experiments with various Hangul document images.

Holographic Interferometry를 이용한 탄성 고무 인상재의 체적변화에 관한 연구

하치양,이성복,우이형 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.1

This study investigated the time-dependent dimensional changes of elastomeric impression materials using holographic interferometry. Six commercial impression materials, Permlastic(polysulfide), Xantopren VI, (condensation silicone), low and medium viscosity of Exafine, Provil (addition silicone), and Impregum( polyether), were selected. Steel plate was used as custom tray, and each impression specimen was 20 mm in width, 15 mm in length and 3 mm in thickness. Each impression material was evaluated at 30 minutes, 1, 2 4, 6, 8 and 12 hours after setting using real-time holography. The results were as follow 1. Xantopren VL and Permlastic showed relatively severe and continuous dimensional changes after setting. Low viscosity of Exafine, Provil, Impregum showed relatively slight dimensional changes with function of time and medium viscosity of Exafine showed almost no dimensional change from 2 hours after setting to 6 hours. 2. On initial dimensional changes within 1 hour, the amount of change in low viscosity of Exafine was the least and Xantopren VL was the largest. 3. On dimensional changes at 4 hours after setting, the amount 'of change in medium viscosity of Exafine was the least and the change of Xantopren VL was the largest. 4. In overall dimensional stability during 12 hours, medium viscosity of Exafine was the most stable and Xantopren VL and Permlastic were least stable.

상하악 전치부 치열궁 형태에 대한 새로운 접근 : 한국성인 정상교합자 모델에서

하만희,손우성,양훈철 대한치과교정학회 2001 대한치과교정학회지 Vol.31 No.3

상하악 전치부 치열은 치아의 형태 변위, 선천결손 등에 의해 종종 교합관계나 심미성에 문제점을 나타내게 된다. 이러한 문제점을 극복하기 위해 임상의는 전치부 비율을 진단시 이용하게 되나, 치열중 형태, 견치간 폭경(intercanine width), 치열궁 장경(segment depth)과 치열중 둘레(arch perimeter)에 따른 전치부 비율의 변화로 인해 이러한 비율을 전치부 교합관계 예측에 직접 적용하는데는 한계가 있다. 이에 본 연구에서는 한국성인 정상교합자 모델(남자 : 20쌍, 여자 : 20쌍)에서 상하악 전치부 치열궁 형태를 least square method로 조사하였다. 한국인 정상교합자의 상하악 전치부 치열궁 형태는 다항 함수(polynomial function), 베타 함수(beta function), 하이퍼볼릭 코사인 함수(hyperbolic cosine function) 순으로 곡선 접합(curve fitting)하였으며, 이러한 곡선 접합도는 남녀, 상하악에 관계없이 일정하였다. 또한 곡선접합(curve fitting)된 치열궁 형태를 바탕으로 견치간 폭경(intercanine width), 치열궁 장경(segment depth)과 치열궁 둘레(arch perimeter)간의 상관관계를 구하였다. 이러한 상관관계는 견치간 폭경에 따른 치열궁 형태 예측과 보다 정확한 전치부 비율에 대한 정보를 제공할 것이다. (주요 단어 : 전치부 치열궁 형태, 전치부 비율) Maxillary and mandibular anterior dental arches often have the problems of occlusal relation and esthetics by malformations of teeth, congenital missing, et at. Though the clinician usually use the anterior ratio to overcome this problems, he has the limitation of a direct application this ratio to the prediction of anterior occlusal relationship by the change of anterior ratio as dental arch form, intercanine width, segment depth and arch perimeter. So this study examine maxillary and mandibular anterior dental arch forms by least square method using Korean normal occlusion models(man : 20 casts, woman : 20 casts), Maxillary and mandibular anterior dental arches of Korean normal occlusion models are curve fitted to polynomial function, beta function, hyperbolic cosine function in order. And this accuracy of curve fitting is constant regardless of man/woman and maxilla/mandible. The relationships between intercanine width, segment depth, and arch perimeter based on this curve fitted dental arch form are acquired. This relationships will give the prediction of anterior dental arch form and the information of more accurate anterior ratio according to intercanine width. Key words : Anterior dental arch form, Anterior ratio

에스트로젠과 프로게스테론이 골모세포의 증식과 활성에 미치는 영향

하국봉,손우성,김세원 대한치과교정학회 2001 대한치과교정학회지 Vol.31 No.2

치아이동에 대한 생력학적 반응은 골 형성과 재형성의 조합이라 할 수 있다. 골 형성과 흡수에는 국소적으로 작용하는 여러 부분비 인자가 관여한다. 대표적인 여성호르몬인 에스트로젠파 프로게스테론도 그 중의 한 인자로 성인 여성은 생리, 임신, 폐경 등 상태에 따라 체내 성호르몬 농도가 달라진다. 따라서 이러한 농도의 변화에 따라 골조직이 영향을 받을 수 있을 것으로 추정된다. 골모세포는 골흡수를 일으키는 호르몬인 PTH, Vit D3 등에 일차적으로 반응함으로 써 골형성 뿐만 아니라 골흡수에도 일정한 역할을 하고 있어 파골 세포에 영향을 주는 부분비 인자도 추측해 볼 수 있다. 본 연구에서는 ROS17/2.8및 HOS세포주를 배양하면서 에스트로젠 및 프로게스테론 등 여성 호르몬을 처리한 후 골모세포의 증식과 활성에 미치는 영향을 측정하여 다음과 같은 결과를 얻었다. 1.에스트로젠은 HOS 세포의 증식을 억제하였으며 ROS17/2.8 세포의 증식은 촉진하는 것으로 관찰되었다. 2.에스트로젠은 HOS 세포의 alkaline phosphatase 활성을 증가시켰고 ROS 세포에서는 효소활성을 억제하는 것으로 나타났다. 3.프로게스테론은 HOS 및 ROS17/2.8세포 모두의 증식을 억제하였으며 골모세포의 alkaline Phosphatase활성에는 영향을 미치지 못하였다. 4.에스트로젠과 프로게스테론은 골모세포내에서 생성되는 superoxide, nitric oxide 및 gelatinase 활성 등 골모세포의 기능에는 유의한 변화를 일으키지 않았다. Biomechanical reactions of tooth movement are the combination of bone formation and resorption, in which many paracrine factors are involved. The sex hormone is one of the paracrine factors and the sex hormonal level of an adult female varies according to the body condition, e.g. mensturation, pregnancy, Postmenopause, etc. Although the exact mechanism is not clarified yet, estrogen and progesterone are known to regulate the function of osteoblast. Again osteoblast is reported to affect the function of osteoclast. The purpose of this study is to determine the influence of the female sex hormone, estrogen and proresterone, on the cell proliferation and activity of HOS and ROS17/2.8 cell line. The observed results were as follows. 1.Estrogen inhibited HOS cell proliferation and promoted ROS17/2.8 cell proliferation. 2.Estrogen increased the activity of alkaline phosphatase of HOS cell and reduced the activity of alkaline phosphatase of ROS17/2.8 cell. 3.Progesterone inhibited the proliferation of HOS and ROS17/2.8 cell, but had no influence on the activity of alkaline phosphatase. 4.Estrogen and progeterone did not have any particular effects on the activity of super oxide, nitric oxide and gelatinase of HOS and ROS17/2.8 cell.

水稻增收에 關한 硏究

河浩成,金明燦,金正敎,朴愚喆 진주농과대학 1968 진주농과대학 연구논문집 Vol.- No.7

本試驗 結果 米穀 增收의 要件으로 客土와 深耕에 依한 作土層을 增加시킴과 아울러 有機物의 含量을 높이고, 따라서 水?生育에 重要한 要素로 알려진 珪酸質 肥料의 施用 및 不足 ?類를 보충함과 同時에 灌排水 管理 및 耕種關係를 合理的으로 함으로서 現在의 平均 玄米收量 보다 높은 數値의 結果를 얻었다. (1) 客土를 反當 7,500kg 程度 施用함과 有機物 및 ?類 補充으로 莖數, 穗數가 10%, 玄米收量이 12% 增加하였다. (2) 深耕에 依한 作土層의 깊이를 10∼12cm에서 18cm 程度 깊게 함과 同時에 有機物 및 ?類補充으로 客土와 같은 傾向의 成績을 얻었다. (3) 客土와 深耕을 같이 處理한 結果, 客土, 深耕, 單用區에 比하여 莖數, 穗數의 增加는 약간 보았으나 收量面에서는 크게 增加를 보지 못하였다. 이는 當年에 客土, 深耕으로 作土層을 增加시켰다 하드라도, 土壤의 熱度가 잘 이루어 지지 못하였기 때문에 야기된 것으로 본다. (4) 二條竝木區로 坪當 株數를 늘이므로써 收量增加는 認定되지 않았다. 이는 3의 경우와 같다고 하겠으나 他區 역시 坪當 株數가 90株였기 때문으로 본다. To study about an increased yield of rice, this experiment was carried out at five county selected in Kyung Nan province. The essential conditions of increased yield obtained from this experiment were as follows: 1) Supplying with red clay soil to 7,500kg per 10are some of organic matter and mineral nutrition into each field, the stem and ear was increased in number to ten per cent and an unpolished rice to twelve per cent. 2) The coincident results were obtained in deep plowing treatment in order to plow more deep the sub-soil floor of 10∼12㎝ to 18㎝ with supplement of organic matter and mineral nutrition. 3) When the above mentioned two treatments were done together, the stem and ear was moderately increased in number compare with each if the depth of sub-soil was increased according to the supplement of red clay soil and decper plowing, howerer, the soil was not efficiently development during one year for plant to grow well. 4) The two strips planting to increase the number of plant per 0.245 acre was not increased in yield of an unpolished rice. It was seems that the reason also was same as in 3., and that the number of plant was epually ninety in the other all experimental treatment.