RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

체육수업의 재미요인이 학생들의 심리적 유인성향에 미치는 영향

이종철(Lee Jong-Chul),조홍식(Cho Hong-Sik),박윤혁(Park Yoon-Hyuk),이상섭(Lee Sang-Sub) 한국체육과학회 2006 한국체육과학회지 Vol.15 No.3

The purpose of this study was to explore ways to at identifying what factors made students felt enjoyment during physical education class and how much those factors influence on psychological of inducement inclination. To this end, Cluster Random Sampling was used to set up the population with boys and girls middle school students in Kyunggi-do and Inchon-si area. The survey tool was questionnaire and Collected data hereof were analyzed by one way ANOVA analysis and Multiple Regression Analysis with SPSS WIN Ver 11.5. The aforementioned method and procedure led this research to the conclusion as follows. Firstly, out of sub-factors of physical education class enyoyment by middle schoolers' individual characteristics(gender, result), boy students showed more than girl students, in which there were statistically significant difference. Meanwhile, statistically significant difference was not found in free time, explanation and modeling. Secondly, out of sub-factors of psychological of inducement inclination by middle schoolers' individual characteristics(gender, result), boy students' response was higher in senses of confidence, enthusiasm, accomplishment and indulgence than girl students' in which all considers appeared. Thirdly, the effect enjoymant factors of physical education class had statistically significant on sub-factors of psychological of inducement inclination senses of confidence, enthusiasm, accomplishment and indulgence, free time that is a sub-factor of enjoymant which did not had statistically significant influenced on sense of accomplishment.

사관생도의 체육활동을 통한 사회성발달요인이 신체적 자기효능감에 미치는 영향

민창기(Min, Chang-Gi),이종민(Lee, Jong-Min),박상섭(Park, Sang-Sub),박순희(Park, Sun-Hee),김인기(Kim, In-Ki) 한국체육과학회 2014 한국체육과학회지 Vol.23 No.2

The purpose of this study of cadets social development through physical activity factor out the impact of physical self-efficacy are seeing. To this end, on the basis of previous research and theory, cadets according to the individual characteristics of participants in physical activity factors, social development, and physical effects on self-efficacy was determined empirically. The subjects of this study are based in KAFA cadets to participate in physical activity among the 200 selected patients, 169 were used in the final part were used. By the way, more studies were summarized as follows. First, the social development of the sub-factors of the social factors, governance, stability, no statistically significant differences between men and women in the show, and overall social development factors in men than in women was higher. Physical self-efficacy subscale of physical competence and physical attractiveness in the sense of gender showed statistically significant differences, and about the physical self-efficacy was higher in women than men. Second, the social development of the factors involved in the stability of the sub-factors, depending on stocks showed a statistically significant difference, and the overall social development factors were the highest in the group event. Self-efficacy of physical competence subscale of the body involved in the events showed significant differences according to the tatistical and physical competence, the individual events and the most underestimated, interpersonal and organization in order. In other words, the overall physical self-efficacy and the lowest individual events, personal events, corporate events in order, respectively. Third, social development, physical factors impact on self-efficacy to evaluate the results with social dominance physical competence, physical self-expression, sense of physical attractiveness showed a positive correlation, and perceived physical ability and a negative correlation with has shown. Stability in the perceived physical ability, has shown a positive correlation.

흡연에 의한 급성 호산구성 폐렴 2예

박종빈,김학렬,주현준,유태양,신성남,신정현,송정섭,황기은,김소영,양세훈,정은택 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

최근 흡연을 시작한 젊은 성언에서 급성 호산구성 폐렴의 발생에 대한 증례가 보고되고 있다. 급성 호산구성 폐렴은 대개 발열을 동반하기 때문에 초기에는 감염성 폐렴으로 잘못 진단, 치료되는 경우가 많다. 아직 정확한 병태 생리 및 조직학적 소견은 밝혀져 있지 않지만 병력 청취 및 임상 양상, 방사선 소견의 관찰을 통해 급성 호산구성 폐렴을 의심하고, 진단을 위해 기관지 폐포 세척액 검사를 시행한다면 좀 더 쉽게 진단 내릴 수 있을 것이다. 본 저자들은 최근 처음 시작한 흡연에 의해 발생한 급성 호산구성 폐렴을 진단하고, 스테로이드를 투여하여 성공적으로 치료한 환자 2예를 경험하였기에 이를 문헌 고찰과 함께 보고하는 바이다. Acute eosinophilic pneumonia(AEP) is characterized by acute febrile respiratory illness associated with diffuse pulmonary infiltration and pulmonary eosinophilia. The specific etiology for acute eosinophilic pneumonia is elusive. By some investigators, cigarette smoking is suggested as a causative substance which can cause AEP. In recent, the authors experienced two cases of AEP following cigarette smoking. Both cases had characteristic features including age around 20 years, new onset smoking before occurance of AEP, diffuse infiltration on chest radiography, pulmonary eosinophilia based on bronchoalveolar lavage and acute improvement after steroid therapy. These clinical features are resemble with previous smoking induced AEP case reports. Base on these clinical features, cigarette smoking associated AEP could be diagnosed more easily.

재활 운동프로그램을 통한 요통환자들의 근력과 통증 변화에 미치는 영향

박종성,이영섭,유영규 명지대학교 예체능연구소 2003 藝體能論集 Vol.14 No.-

This study set out to Investigate what kind of changes there were in the muscular strength, pain and distress of life in patients with low back pain(operated patients, non-operated patients) after applying an exercise program for eight weeks, as well as to test the effects of the rehabilitation exercise program in each group of operated and non-operated patients. The subjects were 23 for the operated group(male· 10, female 13) and 22 for the non-operated group(male· 10, female: 12). They were administered with a lumbar extension exercise two times a week. The intensity of the exercise was set at 50% of 1RM, the aerobic exercise at 40-70% and the resistance exercise at 40-80% in two or three sets. The intensity was gradually Increased Before and after applying the exercise program, the subjects were measured in terms of muscular strength for lumbar extension, the degree of pain, and distress of life. The collected data was treated using SPSS/PC V.100. The paired t-test was applied to the changes of each group by the period of the exercise program and to the differences according to the period of treatment for each item measured. The previous examination went through the analysis of covariance, and the acceptance criterion(α) was set at P <05 for the hypothesis. The changes in the muscular strength, pain and distress of life by the exercise program were as follows: The group of operated patients had statistically significant differences (p<01) in the changes of the muscular strength for lumbar extension and the muscular strength for each isometric torque after applying the exercise program. The case was true for both men and women. In the muscular strength ratio for lumbar extension and isometric torque, the men(p<5) and the Women patients (p<1) all showed statistically significant differences, which was also true in the changes of distress of life and pain with the men of p<1 and the women of p<01. The group of non-operated patients had statistically significant differences (p<01) for both men and women in the changes of the muscular strength for lumbar extension and the muscular strength for each isometric torque after applying the exercise program. While the men patients didn't show any statistically significant difference (p<5) in the muscular strength ratio for lumbar extension and isometric torque, the women did (p<1). Both men and women patients in the group had statistically significant differences (p<01) in the changes of distress of life and pain. The changes that happened after applying the exercise program were compared between the two groups of operated patients and non-operated ones. There were no statistically significant differences(p<5) found in the changes of the muscular strength for lumbar extension between the men of the two groups, but the women showed statistically significant differences(p<5) at 0˚and 72˚between the two groups. And there were no statistically significant differences(p<5) in the changes of the muscular strength ratio for lumbar extension and isometric torque, distress of life, and pain between the two groups. Based on the results, after applying the exercise program, both the operated group and the non-operated group significantly increased in the muscular strength for lumbar extension and statistically decreased in pain and distress of life. It is necessary that an exercise program should be provided in diverse ways to treat patients with low back pain in addition to stability, rest, medical therapy, psychotherapy, physical therapy, and operations. In particular, operated patients should be able to have an access to an exercise program that reflects their conditions such as the psychological burden for an operation, remaining pain, and the risk of recurrence due to muscular atrophy. And for non-operated patients, an active and positive exercise program should be provided to improve their muscular strength and to relieve them of pain. It was concluded that the exercise program was effective and made a contribution to improving the muscular strength and reducing distress of life and pain for both the operated patients and the non-operated ones. In the future, a focus needs to be made on developing and applying an appropriate exercise program that can set the intensity, frequency and period of exercise taking into account many elements like the cause and symptoms of low back pain and the patient's characteristics.

선택적 세로토닌 재흡수 억제제(Selective Serotonin Reuptake Inhibitor, SSRI)가 한국인 알코올의존증 환자의 알코올 갈망행동에 미치는 영향과 성격 유형에 대한 분석

이종섭,박청산,배종훈 건국대학교 의과학연구소 1997 건국의과학학술지 Vol.- No.7

선택적 세로토닌 재흡수 억제제(Selective Serotonin Reuptake Inhibitor, SSRI)가 한국인 알코올의존증 환자의 알코올 갈망행동에 미치는

이종섭,배종훈,박청산 건국대학교 의과학연구소 1997 건국의과학학술지 Vol.7 No.-

In our study during 6 weeks, We examined the effect of selective serotonin reuptake inhibitor(SSRI) on the treatment of Korean alcohol dependence patients and analyzed their personality traits for the more effective approach toward therapeutic rehabilitation of them. Results are as followings: 1) Analysis of the results by Obsessive-Compulsive Drinking Scale(OCDS) showed significant decreased effects on alcohol craving behavior of patients with alcohol dependence from 4th week to 6th week in SSRI treatment group compared with placebo group. 2) There was significant decreace of degree of alcohol craving behavior in 6-week SSRI treatment group compared with placebo group in analysis by Likert Scale. 3) Korean alcohol dependence patients showed type I personality traits.

착유우 사료에 대한 Aspergillus oryzae 발효물질 첨가가 in vitro 건물, 섬유소 및 단백질 소화율과 발효액의 pH에 미치는 영향

명윤아,박덕섭,이수기,박종수,김용국 충남대학교 농업과학연구소 2002 농업과학연구 Vol.29 No.2

본 연구는 Aspergillus oryzae 배양물이 in vitro 건물, 섬유소 및 단백질 소화율과 pH에 미치는 영향을 구명하고자 실시하였다. 균종 Aspergillus oryzae는 한국 생명공학연구원에서 분양받았으며, 본 연구에 사용된 사료는 시중 유통중인 착유우용 배합사료와 TMR 사료로서, 일반성분 분석은 (주)우성사료 중앙실험실에서 실시하였고, 건물, 섬유소 및 단백질 소화율 실험은 충남대학교 실험실에서 실시하였다. 배합사료와 TMR에 Aspergillus oryzae 배양물 0, 1.0, 2.0 및 3.0%를 첨가한 후, Holstein종 착유우로부터 채취한 반추위액에 시료 2.0g 내외를 넣어 진탕배양기에서 24, 48 및 72시간 소화시켰다. 침전물에 0.2% pepsin이 들어있는 0.1N HCl 30ml를 넣고 39℃ incubator에서 48시간 소화시키고 나서, 마지막으로 침전물을 60℃의 건조기에서 48시간 건조시켰다. 실험은 3회 반복하여 실시하였다. 건물 소화율은 Aspergillus oryzae 발효물을 첨가하지 않은 대조구에 비해 24시간 배양시킨 배합사료의 경우 2.1%(63.1%), 9.7%(68.5%) 및 9.0%(68%) 개선되었고, TMR은 4.8%(60.0%), 6.4%(61.1%) 및 2.9%(58.8%)의 개선효과가 있었다. 이와 대조적으로, 48시간 및 72시간 배양시킨 시험구의 건물 소화율에 Aspergillus oryzae 배양물이 미치는 영향은 24시간 배양시보다 상대적으로 낮은 경향을 보였다. 섬유소에서 소화율에서 Aspergillus oryzae가 조섬유, ADF 및 NDF 소화율을 유의적으로 개선시킨다는 것을 알 수 있었다. 배합사료에서 3% 첨가구의 경우 24시간 배양시 13.3%(53.3), 72시간 배양시 2.4%(54.6%)까지 증가되었다. 하지만, 첨가수준에 따라 소화율이 높아졌음에도 불구하고 첨가수준별 개선효과에는 유의적인 차이가 없었다. 단백질 소화율은 Aspergillus oryzae 배양물 첨가에 의해 배합사료에서 0.4%(79.7)에서 9.4%(71.8%)의 유의적인 개선효과를 나타내었지만, 2.0%와 3.0% 첨가구간의 유의적인 차이는 인정되지 않았다. TMR의 경우에는 4.0%(70.4%)에서 6.3%(65.1%)의 유의적인 소화율 향상을 나타내었지만 2.0%와 3.0% 첨가구간의 유의적인 차이는 인정되지 않았다. 본 연구에서, pH 처리구간 유의적인 차이는 없었으나, Aspergillus oryzae 배양물 첨가에 따라 pH가 약간 감소하는 경향을 보였다. 그러므로 본 발효물은 pH에는 영향을 미치지 않으나 건물, 섬유소 및 단백질 소화율 향상에는 다소 효과가 있는 것으로 결론을 내릴 수가 있다. This study was conducted to examine the effects of an Aspergillus oryzae fermentation culture on the in vitro digestibilities of dry matter, crude fiber, acid detergent fiber (ADF), neutral detergent fiber (NDF), crude protein and pH in vitro experiment of diets for dairy cows. A fungal species, Aspergillus oryzae was supplied by Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea (KCTC 1229). The experimental diets were commercial compound feed(concentrate) and total mixed ration (TMR) for lactating cows, of which chemical analyses were determined at Research and Development Institute, Woosung Feed Co.. Ltd.. while the digestibilities were done at the laboratory of Chungnam National University. Aspergillus oryzae culture products were added to compound feed and TMR at the rate 0, 1.0. 2.0, 3.0% respectively. The experimental diet with the rumen fluid sampled from Holstein fresian milking cows were used and digested for 24 hrs, 48hrs and 72hrs in the shaking incubator. The residues of the digesta were digested for 48hrs in the incubator in which put 30㎖ of 0.1N HCI with 0.2% pepsin at 39℃. The final precipitates were dried for 48hrs in the drier at 60℃. These experimental procedures were triplicated to determine the in vitro digestibility of dry matter. crude fiber. ADF, NDF, crude protein and pH. Compared to control diet, not added Aspergillus oryzae, the DM digestibility of fungal diets were improved 2.1%(63.1%), 9.7%(68.5%) and 9.0%(68.0%) for 24 hour fermentation in compound feed while 4.8%(60.0%), 6.4%(61.1%) and 2.9%(58.8%) in TMR. On the contrary. for 48 hour and 72 hour digestibilities, the effects of Aspergillus oryzae culture on the digestibility of dry matter were relatively lowered compared to 24 hour digestibility. Referring to the digestibility of dietary fiber, Aspergillus oryzae was believed to significantly improve digestibilities of crude fiber, ADF and NDF. Those were increased up to 13.3%(53.3%) for 24 hour fermentation while 2.4%(54.6%) for 3.0% added for 72 hour fermentation in compound feed. However, there were no signifiant differences among the treatments for the inclusion rate of Aspergillus oryzae, even though the more inclusion rate, the better digestibility. The protein digestibilities were significantly unproved from 0.4%(79.7%) to 9.4%(71.8%) by adding Aspergillus oryzae into compound feed. However, there were no significant differences between the two experimental diets. 2.0% and 3.0% Aspergillus oryzae included diets. In case of TMR, the protein digestibilities were significantly unproved from 4.0%(70.4%) to 6.3%(65.1%) by adding Aspergillus oryzae. However, there were no significant differences between the two experimental diets, 2.0% and 3.0% Aspergillus oryzae included diets. In this study, there were no significant differences among the treatments in pH. On the contrary, there were slightly decrease in pH by adding Aspergillus oryzae into experimental diets but not significant. Summarizing the results of this examination, Aspergillus oryzae fermentation culture is believed to improve the digestibilities of dry matter, fiber and crude protein in cattle diets. However, more detailed research for the mechanism of the fungal culture is required to improve ruminal environment.

토끼에서 enrofloxacin과 enrofloxacin acetate의 比較藥物動態學

윤효인,박승춘,김창식,김민규,최기섭,신광순,박종일,조준형,조명행 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

This study was designed to examine the in vitro antibacterial activities and pharmacokinetics of enrofloxacin acetate (ENFXA) and enrofloxacin (ENFX). Two enrofloxacins had wide spectrum antibacterial activities against Gram positive and negative bacteria, and mycoplasma, showing potent antibacterial activities with low MICs (0.05-3.33 ㎍/㎖ for Gram positives, 0.002 ㎍/㎖ for Gram negatives and 0.02 ㎍/㎖ for mycoplasma). In order to investigate the profiles of two formulations of enrofloxacin we studied comparative pharmacokinetics of them in rabbits. Five rabbits were given 5 ㎎/㎏ body weight of each enrofloxacin formulation intravenously (i.v.) and orally (p.o.) in a cross-over study. Pharmacokinetic parameters of both formulations were calculated by the use of PCNONLIN, a computer program. Their bioavailibility in rabbits, the means of the area under the curve (AUC). After oral administration of each formulation in rabbits, the mean elimination half-lives (t_1/2,ke) were 3.25h (ENFX) and 4.32 h (ENFXA), and mean AUC 4.27 ㎍·h/㎖ (ENFX) and 6.21 ㎍·h/㎖ (ENFXA). Both enrofloxacin formulations seemed to have good tissue distribution and penetration as indicated by large volume of distribution: 4.76 1/㎏ for ENFX and 7.23 1/㎏ for ENFXA. With the results obtained in this study, ENFXA could be used in place of ENFX in rabbits.

고 무기질 음료(π-water) 섭취시 럭비선수들의 근력, 근 지구력 및 유산소성 운동능력에 미치는 영향

손두옥,선우섭,윤우상,유재충,박영진,오종환 경희대학교 사회체육연구소 1996 體育科學論叢 Vol.- No.9

Most of elite sports player during the sports competing and trainning have used the mineral supplements to enhance on the human performance and, also a number of researcher have recommended the mineral supplements of RDA(Recommended Dietary Allowance) for them. However, It has not exactly known to the scientific interpretation and evidences of relationship between human performance and mineral. Thence, The purpose of this study was to prove whether the mineral supplements of high RDA could enhance the sport player's performance or not The subjects of this study were composed of sixteen Rugby football players(experimental group=8, high density multimineral water intake for 30days; control group=8, placebo intake)in K-H University. Both of groups were tested three times(pre=before intake, mid=after 15days supplements, post=after 30days supplments) about maximum anaerobic power(MAnp) and maximum oxygen uptake by bicycle ergometer(868), muscle strength and muscle endurance by isokinetic Cybex system(350). The statistical analysis employed this study was t-test by statistic 512+ program in machintosh computer. The result of this study were appeared that the difference in control and experimental group signified that the experimental group was high more than the control group in left knee extensor's peak torque at mid-test(P<.05), in left knee extensor's total work(%BW) at mid-test(P<.05), in left extensor's average power and average power(%BW) at mid-test(P<.05). Relative maximal oxygen uptake(㎖/LBMkg/min) in experimental group was higher than control group after 30 days the mineral supplements. These results suggest that the high density multimineral water intake should influence partially rugby football player's performance by the increment of muscle endurance and maximal oxygen uptake.