Rat H-Y 抗體가 생쥐 수정란의 발달단계에 따른 체외배양에 미치는 영향

鄭場龍,朴喜成,朴玉潤 진주산업대학교 농업기술연구소 1993 農業技術硏究所報 Vol.6 No.-

本 硏究는 免疫學的 방법에 의하여 性을 調節할 수 있는 방법을 개발하고자 近郊系統의 Wistar흰쥐의 수컷비장을 摘出하여 均質化시킨 다음 상충액을 취하여 同種의 암컷 腹膣內에 接種함으로서 H-Y抗原에 대한 H-Y 抗 血淸을 제조하였으며, 제조된 H-Y抗 血淸과 함유한 培養液에 ICR系統의 생쥐로부터 각 발달단계별로 채란한 수정란을 NaHCO3-BMOC-3 배양액에 H-Y항 혈청과 보체를 첨가하여 CO_2 incubator에서 24∼30시간 배양을 실시하였다. 본 연구에서 얻은 결과는 다음과 같다. H-Y 抗體의 形成은 rat 10마리중 8마리에서 抗體가 형성되어 80%의 形成率을 보였으며, 427개의 수정란을 H-Y 抗體를 處理하여 이중 182개(43%)가 파괴 되었다. H-Y 抗血淸 및 補體의 存在下에서 培養된 2-, 4-, 8-, 16-세포기, 상실배 및 胞胚期 수정란의 파괴율은 각각 0.0, 42.9, 54.1, 47.0, 48.1 및 47.5%로서 2-세포기와 4-세포기 以上間에는 유의적 (P<0.05)인 差異가 있었으나, 4-세포기 이상과 胞胚期 受精卵間에는 H-Y 항체가 미치는 영향은 유의적(P<0.05)인 차이가 없었다. This experiment was carried out to develop a new technique of identifying XX or XY-bearing mouse embryos by immunological method. H-Y antiserum prepared in inbred Wistar female rats by repeated immunization with spleen cells from males of the same strain. The reactivity of H-Y antibody was confirmed by culturing mouse embryos in the medium containing H-Y antiserum and complement obtained from the guinea pig. The optimal condition for the activity of H-Y antibody was also investigated by culturing embryos under the concentration or affected H-Y antibody and in vitro culture rate. The bilological test with the 8- or 16-cell stge embryos showed H-Y antibody was formed in all female rats immunized with spleen cell, but it was formed only in 80% female rats immunized with the antigen. When the embryos were in vitro cultured in the medium of H-Y antiserum and complement the lysis rate of 2-, 4-, 8-, 16-, morula and blastocyst was 0.0, 42.9, 54.1, 47.0, and 47.5% respectively, but significantly(P<0.05) low than that of 2-cell stage.

Serum Bone Morphogenic Protein-4 Contributes to Discriminating Coronary Artery Disease Severity

Park, Chul Soo,Hong, Oak-Kee,Kim, Mee Kyoung,Chung, Woo Baek,Choi, Yun Seok,Baek, Ki-Hyun,Song, Ki-Ho,Lee, Man Young,Kwon, Hyuk-Sang Williams & Wilkins Co 2015 Medicine Vol.94 No.39

<P><B>Abstract</B></P><P>Bone morphogenic protein 4 (BMP-4) is a known pro-inflammatory and pro-atherogenic cytokine. Here, we investigated whether the serum BMP-4 level predicts coronary artery disease (CAD) severity in humans.</P><P>We measured serum BMP-4 concentrations in 1044 consecutive patients who underwent elective coronary angiography and percutaneous coronary intervention. CAD severity was estimated by the number of diseased vessels showing ≥50% diameter stenosis.</P><P>Among males, the serum BMP-4 level was significantly lower in patients with multivessel disease (MVD) compared with those with single-vessel disease (SVD) (16.3 ± 22.6 vs. 22.0 ± 28.4 pg/mL, <I>P</I> < 0.01). After adjustment for other cardiovascular risk factors, a high serum BMP-4 level was an independent predictor for a decreased risk of MVD (odds ratio, 0.992; 95% confidence interval [CI], 0.985–0.998; <I>P</I> = 0.01) and patients in the lower tertile were 1.55-fold more likely to have MVD compared with upper tertile patients. Receiver-operating characteristic curve analysis demonstrated that the serum BMP-4 level had a 54% sensitivity and 54% specificity for predicting MVD (area under the curve [AUC], 56.5%; 95% CI, 51.9–61.0%; <I>P</I> < 0.01). Serum BMP-4 improved the predictive capability of risk factors for MVD (AUC with and without BMP-4: 64.9 and 63.6%, respectively). Considering the likelihood ratio and number of parameters, adding the serum BMP-4 level provided a better-fit model for predicting MVD compared with the model consisting of conventional risk factors (likelihood ratio <I>χ</I><SUP>2</SUP> = 6.20, <I>P</I> = 0.01). However, an association between serum BMP-4 and CAD was not observed in females.</P><P>Serum BMP-4 levels are independently associated with CAD severity and contribute to discriminating CAD severity in males.</P>

Installing kelp forests/seaweed beds for mitigation and adaptation against global warming: Korean Project Overview

Chung, Ik Kyo,Oak, Jung Hyun,Lee, Jin Ae,Shin, Jong Ahm,Kim, Jong Gyu,Park, Kwang-Seok Oxford University Press 2013 ICES journal of marine science Vol.70 No.5

<P> Seaweed beds can serve as a significant carbon dioxide (CO<SUB>2</SUB>) sink while also satisfying global needs for food, fodder, fuel, and pharmaceutical products. The goal of our Korean Project has been to develop new baseline and monitoring methodologies for mitigation and adaptation within the context of climate change. Using innovative research approaches, we have established the Coastal CO<SUB>2</SUB> Removal Belt (CCRB), which comprises both natural and man-made plant communities in the coastal region of southern Korea. Implemented on various spatial–temporal scales, this scheme promotes the removal of CO<SUB>2</SUB> via marine forests. For example, when populated with the perennial brown alga <I>Ecklonia</I>, a pilot CCRB farm can draw down ∼10 t of CO<SUB>2</SUB> per ha per year. This success is manifested by an increment in biomass accumulations and a decrease in the amount of dissolved inorganic carbon in the water column.</P>

유. 소아에서의 미추마취 적용

박광원,김원옥,조정현,남용택 대한마취과학회 1989 Korean Journal of Anesthesiology Vol.22 No.6

Although regional anesthesia has many advantages over general anesthesia it is not so popular in pediatric patient because of (1) anatomic difference with that of adult (2) lack of patient cooperation (3) difficulty for determination of local anesthetic amount. Among many kinds of regional anesthesia caudal anesthesia is simple, high success rate and needless patient's cooperation with lesser complications. We tried caudal anesthesia in 103 patients from birth to 10 years old children for operation below the inguinal region. We injected 1% lidocaine 10 mg/kg or less for short procedure and 1% lidocaine with 0.5% bupivacaine with 1:1 mixture at the same volume with that of 1% lidocaine only. The results were as follows: 1) Pediatric caudal anesthesia is simple and easy with high success rate (more than 93%). 2) There was one case of systemic onvulsion after lidocaine injection and it seemed to be due to multiple blood aspiration during caudal puncture rather than lidocaine overdose. 3) Four children revealedbradycardia below 70% of control heart rate. This bradycardia probably due to cardiac toxicity of bupivacaine because all of the bradycardia children used bupivacaine. 4) Poor risk children for general anesthesia such as upper respiratory tract infection, abnormal liver function test or congenital heart disease were sixteen among 103 children, and they all proceeded operative procedure without event. 5) Postoperative pain control was excellent in caudal anesthesia than general anesthesia according to pain-discomfort scale with 1 point vs 6 points at recovery room. With the above results we consider that pediatric caudal anesthesia is a safe and useful procedure with lesser complication especially in poor risk children

渦電流 標潗浸透 깊이·表皮效果와 缺陷信號 位相角의 關係解析

鄭泰彦,張基玉,朴大英 韓國非破壞檢査學會 1985 한국비파괴검사학회지 Vol.5 No.1

An experiment to invertigate the rate of change of phase angle of eddy current output signal caused by outer surface defect of nonferromagnetic tube by variation of standard depth of penetration and variation of percent of tube wall penetration was carried out. The results of the experiment show that the phase angle of defect signal is increased with decreasing the standard depth of penetration or the depth of defect. The results also show that the phase angle is decreased with increasing the skin effect of eddy current, and that the resolution is decreased with decreasing the depth of defect.

기관내 삽관마취중 발생한 기도폐쇄

정경숙,박광원,길혜금,김원옥 대한마취과학회 1984 Korean Journal of Anesthesiology Vol.17 No.2

뇌졸증 환자에 대한 임상적 관찰 및 간호

박정옥,Park Chung Oak 대한간호협회 1969 대한간호 Vol.8 No.6

High Frequency Ventilation for Suspension Laryngomicrosurgery under General Anesthesia

Kim, Won Oak,Kim, Jong Rae,Park, Kwang Won,Cho, Chung Hyun Yonsei University, College of Medicine 1986 Yonsei medical journal Vol.27 No.1

성인에서 간혈관종에 의해 발생한 카사바-메리트 증후군

옥찬영 ( Chan Young Oak ),전충환 ( Chung Hwan Jun ),조은애 ( Eun Ae Cho ),이두현 ( Du Hyun Lee ),조성범 ( Sung Bum Cho ),박창환 ( Chang Hwan Park ),주영은 ( Young Eun Joo ),김현수 ( Hyun Soo Kim ),류종선 ( Jong Sun Rew ),최성규 ( 대한소화기학회 2016 대한소화기학회지 Vol.67 No.4

Hemangiomas are the most common benign tumors of the liver. They are generally asymptomatic, but giant hemangiomas can lead to abdominal discomfort, bleeding, or obstructive symptoms. Kasabach-Merritt syndrome is a rare but life-threatening complication of hemangioma, characterized by consumptive coagulopathy with large vascular tumors. More than 80% of Kasabach-Merritt syndrome cases occur within the first year of life. However, there are few reports of Kasabach-Merritt syndrome with giant hepatic hemangioma in adults and, as far as we know, no reports of Kasabach-Merritt syndrome with hepatic hemangioma treated with first line medical treatment only. The most important treatment for this syndrome is removal of the large vascular tumor. However, surgical treatment entails risk of bleeding, and the patient’s condition can mitigate against surgery. We herein present a case of unresectable giant hepatic hemangioma with disseminated intravascular coagulopathy. The patient was a 60-year-old woman who complained of hematochezia, ecchymosis, and abdominal distension. She refused all surgical management and was therefore treated with systemic glucocorticoids and beta-blockers. After two weeks of steroid therapy, she responded partially to the treatment. Her laboratory findings and hematochezia improved. She was discharged on hospital day 33 and observed without signs of bleeding for three months. (Korean J Gastroenterol 2016;67:220-223)

Vecuronium 분할투여에 의한 기관내삽관의 임상적 평가

신양식,박광원,김혜란,김원옥,조정현 대한마취과학회 1987 Korean Journal of Anesthesiology Vol.20 No.4

In the clinical situations which the use of SCC for endotracheal intubation is contraindicated, we have currently tried to find some ideal substitutes for SCC. One of these, neuromuscular blockade will occur sooner if the intubating dose of a non-depolarizing drug is proceded by a small, subparalyzing initial dose. Our previous studs for the divided doses of pancuronium was concluded to be a substitute for SCC. However, the slow onset and long duration of pancuronium were net suitable in some clinical applications. This study was undertaken to eatimate a newer shorter acting relaxant, vecuronium, in divided doses to apply for rapid sequence endotracheal intubation. The subparalyzing dose, 0.01 mg/kg, of vencuronium 4 min. prior to its intubating dose, 0.1 mg/kg, for all patients was administered under the monitoring of TOF response with Relaxograph. The intubating dose of vecuronium followed immediately the induction agent, thiopental sodium (5 mg/kg) . Orotracheal intubation was done 90 sec after the intubating dose. Intubation conditions and TOF responses were evaluated. The results are as follows : 1 ) After the priming dose, twenty-six patients complained of minor aide effects. 2) There was no difficultly in intubation. Forty·mix patients(94%) were distributed in 1 and 2 grade of intubation condition. 3) In the grade 1, female patients were more distrihuted than male patients(p$lt;0.05) . 4) The responses of TOF at the intubation were 44.54±38,92%. There were wide indivi-dual variances. 5) The internal between the intubating dose and the disappeared twitch response was 3.72±2.73 min. The first twitch reappeared 26.04±10.86 min. after the intubating dose. In conclusion, we recommend that the divided doses of vecuronium(the priming and intubating doses, 0.01 mg/kg and 7.1 mg/kg, respectively) for the rapid sequence endotracheal intubation may be applied adequately in some clinical situations.