e-Pharmacophore modeling and in silico study of CD147 receptor against SARS-CoV-2 drugs

Nisha Kumari Pandit,Simranjeet Singh Mann,Anee Mohanty,Sumer Singh Meena Korea Genome Organization 2023 Genomics & informatics Vol.21 No.2

Coronavirus has left severe health impacts on the human population, globally. Still a significant number of cases are reported daily as no specific medications are available for its effective treatment. The presence of the CD147 receptor (human basigin) on the host cell facilitates the severe acute respiratory disease coronavirus 2 (SARS-CoV-2) infection. Therefore, the drugs that efficiently alter the formation of CD147 and spike protein complex could be the right drug candidate to inhibit the replication of SARS-CoV-2. Hence, an e-Pharmacophore model was developed based on the receptor-ligand cavity of CD147 protein which was further mapped against pre-existing drugs of coronavirus disease treatment. A total of seven drugs were found to be suited as pharmacophores out of 11 drugs screened which was further docked with CD147 protein using CDOCKER of Biovia discovery studio. The active site sphere of the prepared protein was 101.44, 87.84, and 97.17 along with the radius being 15.33 and the root-mean-square deviation value obtained was 0.73 Å. The protein minimization energy was calculated to be -30,328.81547 kcal/mol. The docking results showed ritonavir as the best fit as it demonstrated a higher CDOCKER energy (-57.30) with correspond to CDOCKER interaction energy (-53.38). However, authors further suggest in vitro studies to understand the potential activity of the ritonavir.

Optimal panel of immunohistochemistry for the diagnosis of B-cell non-Hodgkin lymphoma using bone marrow biopsy: a tertiary care center study

Nisha Marwah,Manali Satiza,Niti Dalal,Sudhir Atri,Monika Gupta,Sunita Singh,Rajeev Sen 대한혈액학회 2021 Blood Research Vol.56 No.1

Background Morphological diagnosis of non-Hodgkin lymphoma (NHL) is usually based on lymph node biopsy. Bone marrow biopsy (BMB) is important for staging, and morphology alone can be challenging for subtyping. Immunohistochemistry (IHC) allows a more precise diagnosis and characterization of NHL using monoclonal antibodies. However, there is a need for a minimal panel that can provide maximum information at an affordable cost. Methods All newly diagnosed cases of B-cell NHL with bone marrow infiltration between 2017 and 2019 were included. BMB was the primary procedure for diagnosing B-cell NHL. Subtyping of lymphomas was performed by immunophenotyping using a panel of monoclonal antibodies on IHC. The primary diagnostic panel of antibodies for B-cell NHL included CD19, CD20, CD79, CD5, CD23, CD10, Kappa, and Lambda. The extended panel of antibodies for further subtyping included CD30, CD45, CD56, Cyclin D1, BCL2, and BCL6. Results All cases of B-cell NHL were classified into the chronic lymphocytic leukemia (CLL) and non-CLL groups based on morphology and primary IHC panel. In the CLL group, the most significant findings were CD5 expression, CD23 expression, dim CD79 expression, and weak surface immunoglobulin (Ig) positivity. In the non-CLL group, they were CD5 expression, positive or negative CD23 expression, strong CD79 expression, and strong surface Ig expression. An extended panel was used for further subtyping of non-CLL cases, which comprised CD10, Cyclin D1, BCL2, and BCL6. Conclusion We propose a two-tier approach for immunophenotypic analysis of newly diagnosed B-cell NHL cases with a minimum primary panel including CD5, CD23, CD79, Kappa, and Lambda for differentiation into CLL/non-CLL group and Kappa and Lambda for clonality assessment. An extended panel may be used wherever required for further subtyping of non-CLL.

Tunable Release of Combined Contraceptive Steroids from Coreshell Gelatin/PCL Fibers

Nisha U,Merline C,Lakshminarayanan Ragupathy,Diksha Painuly 한국섬유공학회 2020 Fibers and polymers Vol.21 No.9

The present study investigates simultaneous release of two hydrophobic contraceptive steroids from core-shellfibers made by coaxial electrospinning. The contraceptive steroids levonorgestrel (LNG) and ethinylestradiol (EE) wereincorporated in gelatin/poly(ε-caprolactone) (PCL) core-shell fibers. The influence of shell concentration and core feed/flow rate (ml/h) on the physical, chemical, mechanical and release properties of drug incorporated coaxial fibers wereevaluated using scanning electron microscopy (SEM), transmission electron microscopy (TEM), differential scanningcalorimetry (DSC), Fourier transform infrared (FT-IR) spectroscopy, universal testing machine (UTM) and highperformanceliquid chromatography (HPLC). SEM results revealed the influence of feed/flow rate on pore size (in the range586-1036 nm) and fiber diameter (i.e. 621-1650 nm) of the coaxial fibers. TEM analysis confirmed the presence of coreshellmorphology. DSC results conferred that drugs were in an amorphous form within core-shell fibers. The FT-IR spectraestablished the drug encapsulation by the electrospinning process. Swelling studies demonstrate that increasing the shell(PCL) concentration i.e. 4-10 % w/v decreases the swelling ratio (295-140 %). The drugs release kinetics satisfactorilydescribed by first-order (R2>0.95) model and Korsmeyer-Peppas model (R2≥0.95) for all the prepared core-shellformulations. These formulations were found to follow anomalous non-Fickian transport, which suggests that the drugrelease is controlled by both diffusion and erosion of polymer matrix. These results clearly demonstrate that it is possible tocontrol the release rate for the two hydrophobic (contraceptive) drugs through coaxial electrospinning process for the firsttime to the best of our knowledge.

Synergistic Multi Enzyme Platform for Isopropanol Detection Using Electrochemical Biosensing Assay

Nisha BHARDWAJ,Myeong-Eun LEE,Young Jin KO,Sung Ok HAN 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

The electrochemical biosensors with enzymes have always been an attractive research topic in various sectors of biology due to their high specificity and reproducibility. In this study, we suggest a novel cellulosome scaffolding based multi-enzyme platform for the detection of isopropyl alcohol (IPA) using electrochemical biosensing assay. Three enzymes namely alcohol dehydrogenase (ADH), NADH oxidase (NOX), and peroxidase were isolated and successfully overexpressed in E.coli BL21 Rosetta respectively. The ability of enzymes to catalyze NAD+/NADH was confirmed using the absorption spectrum at 340㎚ wavelength. From the results, it was confirmed that the rate of oxidization of NADH by NOX and the rate of reduction of NAD+ by ADH were approximately 3 folds compared to control. In the synergistic reaction, comparative to the individual curves i.e. logistic for ADH and decaying for NOX, a straight line parallel to the baseline was obtained which confirmed that NAD+ was reduced to NADH and can be used by NOX for further oxidation simultaneously. Taken together, this study can be a progression in the area of electrochemical biosensors for the detection of IPA.

Direct Organogenesis in Geophila reniformis D. Don., an Important Medicinal Herb

Nisha, A.,Narasimhan, S.,Manjula, S.,Nair, G.M. The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.3

Adventitious multiple shoots were developed from leaf, petiole and internode explants of Geophila reniformis D. Don. on MS medium supplemented with various concentrations of $N^6$-benzylaminopurine (BAP) or Kinetin (KIN) alone or in combination with indole-3-acetic acid (IAA). Leaf showed maximum organogenetic potential, followed by petiole and internode. Murashige and Skoog (MS) medium supplemented with 22.22 $\mu{M}$ BAP and 4.57 $\mu{M}$ IAA induced maximum shoot buds from leaf explants. Internodal segments showed low potential of direct organogenesis. The regenerated shoots rooted the best in presence of 10.75 - 13.44 $\mu{M}$ $\alpha$-naphthalene acetic acid (NAA) along with 2.22 $\mu{M}$ BAP, and were successfully established in the field with a survival rate of 89.11%.

Why do women’s cooperative societies languish? A study of selected societies in Kottayam, Kerala

Nisha Velappan NAIR 이화여자대학교 아시아여성학센터 2015 Asian Journal of Women's Studies(AJWS) Vol.21 No.2

Kerala has had a long history of cooperatives starting from the pre-independence era. The idea of women’s cooperatives came much later, with the earliest ones coming into being only after 1975. This study draws on both primary and secondary data, focusing on the challenges faced by women’s cooperative societies (WCSs) in Kottayam District in Southern Kerala. Most women’s cooperatives function by limiting their operations to small scale banking activities and enlisting men’s participation in various ways. The paper concludes that the scope of empowerment of women through women’s cooperatives is severely constrained by the way property rights and gender relations are structured in the state and the manner in which local male politicians have manipulated the societies for partisan ends.

In vitro antiproliferative study of curcumin loaded nano zeolitic imidazolate framework hybrid biomaterials on HeLa cells

Nisha G. Pillai,Archana K,이경엽,박수진,Asif A 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.79 No.-

Curcumin is a bioactive molecule having potential applications in medicalfield. Present study focuses tosynthesise the hybrid biomaterials of curcumin loaded nano zeolitic imidazolate frameworks of zinc andcobalt metals by solvothermal method. Influence of curcumin on surface morphology and thermalproperties of the hybrid biomaterials have been investigated. The framework hybrids are characterised byFTIR, XRD, SEM, TEM, BET surface area and TGA. The studies reveal that the hybrid biomaterials show highthermal stability with well identified morphology, decrease in particle size and large pore volume. Invitro antiproliferative studies corroborate that hybrid biomaterial displays greater cell growth inhibitoryperformance than that of pure metal organic framework and pure curcumin towards HeLa cells. In vitrocytotoxicity studies reveal that CCM–ZIF-67 hybrid biomaterial exhibits more specificity towardscancerous cells.

Effects of Spatial Training Paradigms on Auditory Spatial Refinement in Normal-Hearing Listeners: A Comparative Study

Nisha Kavassery Venkateswaran,Kumar Ajith Uppunda 대한청각학회 2022 Journal of Audiology & Otology Vol.26 No.3

Background and Objectives: This study compared the effectiveness of two spatial training programs using real and virtual sound sources in refining spatial acuity skills in listeners with normal hearing.Subjects and Methods: The study was conducted on two groups of 10 participants each; groups I and II underwent spatial training using real and virtual sound sources, respectively. The study was conducted in three phases: pre-training, training, and post-training phases. At the pre- and post-training phases, the spatial acuity of the participants was measured using real sound sources through the localization test, and virtual sound sources through the virtual acoustic space identification (VASI) test. The thresholds of interaural time difference (ITD) and interaural level difference (ILD) were also measured. In the training phase, Group I participants underwent localization training using loudspeakers in free field, while participants in Group II were subjected to virtual acoustic space (VAS) training using virtual sound sources from headphones. Both the training methods consisted of 5-8 sessions (20 min each) of systematically presented stimuli graded according to duration and back attenuation (for real source training) or number of VAS locations (for virtual source training).Results: Results of independent t-scores comparing the spatial learning scores (pre vs. post-training) for each measure showed differences in performance between the two groups. Group II performed better than Group I on the VASI test, while the Group I out-performed Group II on the ITD. Both groups improved equally on the localization test and ILD.Conclusions: Based on the present findings, we recommend the use of VAS training as it has practical implications due to its cost effectiveness, need for minimal equipment, and end user usefulness.

Capitalism: A Ghost Story by Arundhati Roy, Chicago: Haymarket Books, 2014.

Nisha Mary Mathew 부산대학교 한국민족문화연구소 2016 Localities Vol.6 No.-

BCL2 antagonist of cell death kinases, phosphatases, and ovarian cancer sensitivity to cisplatin

Nisha Bansal,Douglas C. Marchion,Elona Bicaku,Yin Xiong,Ning Chen,Xiaomang B. Stickles,Entidhar Al Sawah,Robert M. Wenham,Sachin M. Apte,Jesus Gonzalez-Bosquet,Patricia L. Judson,Ardeshir Hakam,Johnat 대한부인종양학회 2012 Journal of Gynecologic Oncology Vol.23 No.1

Objective: The BCL2 family proteins are critical mediators of cellular apoptosis and, as such, have been implicated as determinants of cancer cell chemo-sensitivity. Recently, it has been demonstrated that the phosphorylation status of the BCL2 antagonist of cell death (BAD) protein may influence ovarian cancer (OVCA) cell sensitivity to cisplatin. Here, we sought to evaluate how kinase and phosphatase components of the BAD apoptosis pathway influence OVCA chemo-sensitivity. Methods: Protein levels of cyclin-dependent kinase 1 (CDK1) and protein phosphatase 2C (PP2C) were measured by immunofluorescence in a series of 64 primary advanced-stage serous OVCA patient samples. In parallel, levels of cAMP-dependent protein kinase (PKA), AKT, and PP2C were quantified by Western blot analysis in paired mother/daughter platinum-sensitive/resistant OVCA cell lines (A2008/C13, A2780S/A2780CP, Chi/ChiR). BAD pathway kinase CDK1 was depleted using siRNA transfection, and the influence on BAD phosphorylation and cisplatin-induced apoptosis was evaluated. Results: OVCA patient samples that demonstrated complete responses to primary platinum-based therapy demonstrated 4-fold higher CDK1 (p<0.0001) and 2-fold lower PP2C (p=0.14) protein levels than samples that demonstrated incomplete responses. Protein levels of PP2C were lower in the platinum-resistant versus that shown in the platinum-sensitive OVCA cell line sub-clones. Levels of PKA were higher in all platinum-resistant than in platinum-sensitive OVCA cell line sub-clones. Selective siRNA depletion of CDK1 increased sensitivity to cisplatin-induced apoptosis (p<0.002). Conclusion: BAD pathway kinases and phosphatases, including CDK1 and PP2C, are associated with OVCA sensitivity to platinum and may represent therapeutic opportunities to enhance cytotoxic efficacy. Objective: The BCL2 family proteins are critical mediators of cellular apoptosis and, as such, have been implicated as determinants of cancer cell chemo-sensitivity. Recently, it has been demonstrated that the phosphorylation status of the BCL2 antagonist of cell death (BAD) protein may influence ovarian cancer (OVCA) cell sensitivity to cisplatin. Here, we sought to evaluate how kinase and phosphatase components of the BAD apoptosis pathway influence OVCA chemo-sensitivity. Methods: Protein levels of cyclin-dependent kinase 1 (CDK1) and protein phosphatase 2C (PP2C) were measured by immunofluorescence in a series of 64 primary advanced-stage serous OVCA patient samples. In parallel, levels of cAMP-dependent protein kinase (PKA), AKT, and PP2C were quantified by Western blot analysis in paired mother/daughter platinum-sensitive/resistant OVCA cell lines (A2008/C13, A2780S/A2780CP, Chi/ChiR). BAD pathway kinase CDK1 was depleted using siRNA transfection, and the influence on BAD phosphorylation and cisplatin-induced apoptosis was evaluated. Results: OVCA patient samples that demonstrated complete responses to primary platinum-based therapy demonstrated 4-fold higher CDK1 (p<0.0001) and 2-fold lower PP2C (p=0.14) protein levels than samples that demonstrated incomplete responses. Protein levels of PP2C were lower in the platinum-resistant versus that shown in the platinum-sensitive OVCA cell line sub-clones. Levels of PKA were higher in all platinum-resistant than in platinum-sensitive OVCA cell line sub-clones. Selective siRNA depletion of CDK1 increased sensitivity to cisplatin-induced apoptosis (p<0.002). Conclusion: BAD pathway kinases and phosphatases, including CDK1 and PP2C, are associated with OVCA sensitivity to platinum and may represent therapeutic opportunities to enhance cytotoxic efficacy.