Effect of pH on Types of Acidogenic Fermentation of Fruit and Vegetable Wastes

Mingxia Zheng,Mingyue Zheng,Yuanyuan Wu,Hailing Ma,Kaijun Wang 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.2

The effect of pH (4.0, 5.0, and 6.0) on acidification and fermentation of fruit and vegetable wastes was investigated using batch and semi-continuous experiments under mesophilic condition. Results showed that fermentation types change with pH variation. The pH of acidification system containing fruit and vegetable wastes could automatically decrease to 3.0 ~ 4.0. At this pH range, a stable ethanol production was observed, at which ethanol-type fermentation was obtained. Based on the results, the fermentation types were classified into ethanol-type, mixed acid-type, propionic acid-type, and butyric acid-type fermentations, which occurred at pH 4.0 ~ 4.5, 4.5 ~ 5.0, 5.0 ~ 5.5, and 5.5 ~ 6.5, respectively.

Prevalence of Allergic Rhinitis Among Adults in Urban and Rural Areas of China: A Population-Based Cross-Sectional Survey

Ming Zheng,Xiangdong Wang,Mingyu Bo,Kuiji Wang,Yan Zhao,Fei He,Feifei Cao,Luo Zhang,Claus Bachert 대한천식알레르기학회 2015 Allergy, Asthma & Immunology Research Vol.7 No.2

Purpose: The aim of the present study was to compare the prevalence of self-reported and confirmable allergic rhinitis (AR) with positive skin pricktest (SPT) results among adults living in urban and rural areas of China. Methods: Adults from a community in Beijing and a village in Baoding wereselected as representative urban and rural dwellers, respectively. All eligible residents were enrolled from the population register and received aface-to-face interview using modified validated questionnaires. Equal sets of randomly selected self-reporting AR-positive and AR-negative participantswho responded to the questionnaires were also investigated using skin prick tests. Results: A total of 803 participants in the rural area and atotal of 1,499 participants in the urban area completed the questionnaires, with response rates being 75.9% and 81.5% respectively. The prevalenceof self-reported AR of the rural area (19.1%) was significantly higher than that of the urban area (13.5%). The elementary school of educational levelincreased the risk of having AR (adjusted OR=2.198, 95% CI=1.072-2.236) .The positive SPT rates among subjects with self-reported AR in therural and urban areas were 32.5% and 53.3%, respectively; the confirmable AR prevalence of 6.2% and 7.2% among the rural and urban adults, respectively. Conclusions: The prevalence of confirmable AR is similar between rural and urban areas in China, although there is a higher prevalenceof self-reported AR in the former. Purpose: The aim of the present study was to compare the prevalence of self-reported and confirmable allergic rhinitis (AR) with positive skin prick test (SPT) results among adults living in urban and rural areas of China. Methods: Adults from a community in Beijing and a village in Baoding were selected as representative urban and rural dwellers, respectively. All eligible residents were enrolled from the population register and received a face-to-face interview using modified validated questionnaires. Equal sets of randomly selected self-reporting AR-positive and AR-negative participants who responded to the questionnaires were also investigated using skin prick tests. Results: A total of 803 participants in the rural area and a total of 1,499 participants in the urban area completed the questionnaires, with response rates being 75.9% and 81.5% respectively. The prevalence of self-reported AR of the rural area (19.1%) was significantly higher than that of the urban area (13.5%). The elementary school of educational level increased the risk of having AR (adjusted OR=2.198, 95% CI=1.072-2.236) .The positive SPT rates among subjects with self-reported AR in the rural and urban areas were 32.5% and 53.3%, respectively; the confirmable AR prevalence of 6.2% and 7.2% among the rural and urban adults, respectively. Conclusions: The prevalence of confirmable AR is similar between rural and urban areas in China, although there is a higher prevalence of self-reported AR in the former.

Renal-targeting peptide-microRNA nanocomplex for near IR imaging and therapy of renal ischemia/reperfusion injury

Yongtao Lin,Mingyue Zhao,Lin Bai,Hailun Li,Yong Xu,Xiang Li,Juan Xie,Yiyuan Zhang,Donghui Zheng 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.97 No.-

Renal ischemia-reperfusion injury (RI/R) is one of the main causes of acute renal injury and a commonclinical disease with high morbidity and mortality. It is of great significance to deliver microRNAs(miRNAs) to cells and in vivo to realize gene regulation and treatment of related diseases. In this study, wereported that the nanocomplex FMN-17 could realize both therapeutic and functional monitoringsimultaneously in vivo and in vitro. The nanocomplex comprised a cationic cell-penetrating peptidenona-arginine, a targeting ligand folic acid, a caspase-3 responsive moiety, and a Cy imaging moiety. Thenanocomplex FMN-17 has been shown to deliver miR-17-5p efficiently and selectively into HK-2 cells andtissues. Treatment of HK-2 cells with the nanocomplex significantly increased the miR-17-5p level andinhibited apoptosis, as evident by reducing the expression of active caspase-3 and reactive oxygenspecies. Uptake of FMN-17 in vivo alleviated renal tissue injury by histological assessment. In summary,we designed and synthesized a new miRNA delivery system with high transfection efficiency, goodtherapeutic effect, and near-infrared imaging in renal ischemia/reperfusion injury.

A Novel Natural Dye Derivative for Natural Fabric Supercritical Carbon Dioxide Dyeing Technology

Jinsong Wu,Hongjuan Zhao,Mingyue Wang,Weiru Zhi,Xiaoqing Xiong,Laijiu Zheng 한국섬유공학회 2019 Fibers and polymers Vol.20 No.11

The supercritical carbon dioxide (SC-CO2) dyeing technology is a green dyeing technology, and the usage ofnatural dye makes SC-CO2 dyeing technology safer and more environment-friendly. Nevertheless, after using natural dye inSC-CO2 dyeing, the color depth and fastness of dyed natural fabric are poor. In this study, alkyl and hydroxyalkyl groupswere grafted onto alizarin, which is a natural dye, to elevate the color depths and fastness of alizarin-derivative-dyed naturalfabric. The results demonstrate that the color depths of alkyl-alizarin-dyed and hydroxyalkyl-alizarin-dyed natural fabricswere increased. This has to do with the increase in the solubilities of alkyl alizarin and hydroxyalkyl alizarin in SC-CO2. Thehydroxybutyl-alizarin-dyed wool displayed the best color depth (K/S value: 6.44). And the hydroxyalkyl-alizarin-dyednatural fabric showed good washing fastness and rubbing fastness (about 4-5 level), because that hydroxyalkyl alizarin couldbe linked by a covalent bond to the natural fabric.

Pituitary P62 deficiency leads to female infertility by impairing luteinizing hormone production

Li Xing,Zhou Ling,Peng Guiliang,Liao Mingyu,Zhang Linlin,Hu Hua,Long Ling,Tang Xuefeng,Qu Hua,Shao Jiaqing,Zheng Hongting,Long Min 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

P62 is a protein adaptor for various metabolic processes. Mice that lack p62 develop adult-onset obesity. However, investigations on p62 in reproductive dysfunction are rare. In the present study, we explored the effect of p62 on the reproductive system. P62 deficiency-induced reproductive dysfunction occurred at a young age (8 week old). Young systemic p62 knockout (p62 -/- ) and pituitary-specific p62 knockout (p62 flox/flox αGSU cre ) mice both presented a normal metabolic state, whereas they displayed infertility phenotypes (attenuated breeding success rates, impaired folliculogenesis and ovulation, etc.) with decreased luteinizing hormone (LH) expression and production. Consistently, in an infertility model of polycystic ovary syndrome (PCOS), pituitary p62 mRNA was positively correlated with LH levels. Mechanistically, p62 -/- pituitary RNA sequencing showed a significant downregulation of the mitochondrial oxidative phosphorylation (OXPHOS) pathway. In vitro experiments using the pituitary gonadotroph cell line LβT2 and siRNA/shRNA/plasmid confirmed that p62 modulated LH synthesis and secretion via mitochondrial OXPHOS function, especially Ndufa2, a component molecule of mitochondrial complex I, as verified by Seahorse and rescue tests. After screening OXPHOS markers, Ndufa2 was found to positively regulate LH production in LβT2 cells. Furthermore, the gonadotropin-releasing hormone (GnRH)-stimulating test in p62 flox/flox αGSU cre mice and LβT2 cells illustrated that p62 is a modulator of the GnRH-LH axis, which is dependent on intracellular calcium and ATP. These findings demonstrated that p62 deficiency in the pituitary impaired LH production via mitochondrial OXPHOS signaling and led to female infertility, thus providing the GnRH-p62-OXPHOS(Ndufa2)-Ca 2+ /ATP-LH pathway in gonadotropic cells as a new theoretical basis for investigating female reproductive dysfunction.

miR-148b-3p, miR-190b, and miR-429 Regulate Cell Progression and Act as Potential Biomarkers for Breast Cancer

Wenzhu Dai,Jixiang He,Ling Zheng,Mingyu Bi,Fei Hu,Minju Chen,Heng Niu,Jingyu Yang,Ying Luo,Wenru Tang,Miaomiao Sheng 한국유방암학회 2019 Journal of breast cancer Vol.22 No.2

Purpose: Breast cancer is the most frequently diagnosed malignancy in women worldwide. MicroRNAs (miRNAs) are thought to serve as potential biomarkers in various cancers, including breast cancer. Methods: We evaluated the miRNA expression profiles in 1,083 breast cancer samples and 104 normal breast tissues from The Cancer Genome Atlas database. We used the edgeR package of R software to analyze the differentially expressed miRNAs in normal and cancer tissues, and screened survival-related miRNAs by Kaplan-Meier analysis. A receiver operating characteristic curve was generated to evaluate the accuracy of these miRNAs as molecular markers for breast cancer diagnosis. Furthermore, the functional role of these miRNAs was verified using cell experiments. Targets of candidate miRNAs were predicted using 9 online databases, and Gene Ontology (GO) functional annotation and pathway analyses were conducted using Database for Annotation, Visualization and Integrated Discovery online tool. Results: A total of 68 miRNAs showed significantly different expression patterns between the groups (p < 0.001), and 13 of these miRNAs were significantly associated with poor survival (p < 0.05). Three miRNAs with high specificity and sensitivity, namely, miR-148b-3p, miR-190b, and miR-429, were selected. In vitro experiments showed that the overexpression of these 3 miRNAs significantly promoted the proliferation and migration of MDA-MB-468 and T47D cells and reduced the apoptosis of T47D cells. GO and pathway enrichment analyses revealed that the targets of these dysregulated miRNAs were involved in many critical cancer-related biological processes and pathways. Conclusion: The miR-148b-3p, miR-190b, and miR-429 may serve as potential diagnostic and prognostic markers for breast cancer. This study demonstrated the roles of these 3 miRNAs in the initiation and progression of breast cancer.

Magnetic Field-Driven and Photocured Rapid Shaping of Carbonyl Iron Particles in the Coating to Achieve Superhydrophobic Fabric

Duchao Feng,Luyang Qiao,Guangdong Sun,Kangjing Hong,Mingyue Wu,Chenglong Wang,Jinhuan Zheng 한국섬유공학회 2023 Fibers and polymers Vol.24 No.10

A magnetic field-driven photocurable superhydrophobic fabric coating technology was developed in this study. The coating, consisting of photoreactive carbonyl iron powder (CIP) assembled hydrophobic micro-nano structures, was constructed on the surface of polyester fabric by combining the magnetic field-driven assembly technology and blue light photocuring technology. Camphorquinone (CQ), ethyl 4-dimethylaminobenzoate (EDB) and diphenyliodonium hexafluorophosphate (DPI) were served as the photoinitiator formulation, while polyurethane diacrylate and hydroxyethyl acrylate (HEA) were used as the polymerization-cross-linking matrix for photocurable coating. Photo-differential scanning calorimetry (Photo-DSC) and rheometry were used to characterize the performance of coating formulations. Optical contact angle meter and scanning electron microscope (SEM) were used in combination with tensile performance test to explore the construction of superhydrophobic structure on coating surface induced by magnetic field. The optimal formulation of photoinitiators for coatings with excellent photopolymerization performance has been definitively established. Photocured coating film displayed a distinct hydrophobicity (the water contact angle is approx. 152.7°, and the sliding angle is approx. 7.2°) when the mass ratio of CIP to polymeric components was 1:1. The coated fabric eventually exhibited a satisfactory hydrophobicity (the water contact angle is approx. 153.3°) and mechanical properties.

Structure-Based Design and Synthesis of C-1- and C-4-Modified Analogs of Zanamivir as Neuraminidase Inhibitors

Feng, Enguang,Shin, Woo-Jin,Zhu, Xuelian,Li, Jian,Ye, Deju,Wang, Jiang,Zheng, Mingyue,Zuo, Jian-Ping,No, Kyoung Tai,Liu, Xian,Zhu, Weiliang,Tang, Wei,Seong, Baik-Lin,Jiang, Hualiang,Liu, Hong American Chemical Society 2013 Journal of medicinal chemistry Vol.56 No.3

<P>In order to exploit the 430-cavity in the active sites of neuraminidases, 22 zanamivir analogs with C-1 and C-4 modification were synthesized, and their inhibitory activities against both group-1 (H5N1, H1N1) and group-2 neuraminidases (H3N2) were determined. Compound <B>9f</B> exerts the most potency, with IC<SUB>50</SUB> value of 0.013, 0.001, and 0.09 μM against H3N2, H5N1, and H1N1, which is similar to that of zanamivir (H3N2 IC<SUB>50</SUB> = 0.0014 μM, H5N1 IC<SUB>50</SUB> = 0.012 μM, H1N1 IC<SUB>50</SUB> = 0.001 μM). Pharmacokinetic studies of compound <B>9f</B> in rats showed a much longer plasma half-life (<I>t</I><SUB>1/2</SUB>) than that of zanamivir following administration (po dose). Molecular modeling provided information about the binding model between the new inhibitors and neuraminidase, with the elongated groups at the C-1-position being projected toward the 430-loop region. This study may represent a novel starting point for the future development of improved antiflu agents.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jmcmar/2013/jmcmar.2013.56.issue-3/jm3009713/production/images/medium/jm-2012-009713_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jm3009713'>ACS Electronic Supporting Info</A></P>