다양한 농작업 환경 미세먼지 노출관련 농작업자 면역력 변화

Maharjan Anju(Maharjan Anju ),Acharya Manju(Acharya Manju ),DaEun Lee(DaEun Lee),K.C. Pramod Bahadur(K.C. Pramod Bahadur ),Kusma Sarina(Kusma Sarina ),Adhikari Manisha(Adhikari Manisha ),ChangYul Kim( 한국환경보건학회 2022 한국환경보건학회 학술대회 자료집 Vol.2022 No.5

Evaluation of epithelial transport and oxidative stress protection of nanoengineered curcumin derivative-cyclodextrin formulation for ocular delivery

Pooja Maharjan,Minki Jin,Daseul Kim,JaeWook Yang,Anjila Maharjan,Meong Cheol Shin,Kwan Hyung Cho,김만수,Kyoung Ah Min 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.10

Ocular drug delivery has been a well-knownroute for the drug administration for the treatment of oculardiseases. However, numerous anatomical and physiologicalbarriers prevailing in the eye itself create considerablechallenges for achieving the necessitated therapeutic efficacyalong with ocular bioavailability. However, recentadvances in nanoengineered strategies hold definite promisesin terms of devising improved ophthalmic medicinesfor the effective drug delivery to target the sites withenhanced ocular bioavailability. Curcumin, a hydrophobicpolyphenol yellow colored compound, and its metabolicreduced product, tetrahydrocurcumin (THC), have beenknown for their beneficial pharmacological functions, suchas anti-inflammatory or anti-oxidant activities at varioustissue sites. However, the low aqueous solubility of thesecompounds results in their poor bioavailability, therebylimiting their widespread application. Therefore, in thepresent study, we investigated the changes in drug solubilityby forming inclusion complexes with differentderivatives of hydroxypropyl (HP)-cyclodextrins (CD). To this end, the spray drying technique was used for nanoengineeringcurcumin or THC-loaded formulations toimprove the stability of formulations during the storage. The formulations were characterized in terms of physicochemicalproperties and cellular permeability. The resultsdemonstrated that the encapsulation of curcumin (or THC)into the HP-CDs significantly increased the drug solubilityand enhanced the corneal and retinal epithelial permeability. Curcumin or THC complexes in HP-CDs withimproved bioavailability also induced anti-oxidant activity(SOD1, CAT1, and HMOX1) in higher levels in the ocularepithelial cells and showed oxidative protection effects inrabbit cornea tissues that will boost up their application inocular medicine.

Microstrip Cross-coupled Interdigital SIR Based Bandpass Filter

Maharjan, R.K.,Shrestha, B.,Kim, N.Y. Radioengineering Society 2012 Radioengineering Vol.21 No.3

Feeding behavior and development response of the perilla seed bugs (Nysius sp.) (Heteroptera: Lygaeidae) on multiple crop seeds combinations

Maharjan Rameswor,Yoon Youngnam,Jang Yunwoo,Jeong Mihye,박기도,Yi Hwijong 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.4

The perilla seed bugs, Nysius plebeius Distant and Nysius hidakai Nakatani, are emerging pests of several field crop species including perilla crop in Korea. Here, we investigated the oviposition, nymphal mortality, development, adult emergence, adult longevity, adult weight and female ratio of N. plebeius and N. hidakai on multiple (MC) and double (DC) combinations of five crop seed species: perilla [Perilla frutescens (L.) Britton.], sesame (Sesamum indicum L.), sorghum [Sorghum bicolor (L.)], proso millet (Panicum miliaceum L.), and foxtail millet (Setaria italic L.) in no-choice test. The life-history variables of N. plebeius and N. hidakai were significantly influenced by the crop seed combinations. The most preferred crop seed combinations for oviposition were MC (perilla + sorghum + sesame + proso millet + foxtail millet), followed by DC (perilla + foxtail millet and perilla + sorghum), whereas the least preferred seed combination was DC (proso millet + foxtail millet). Both Nysius sp. were developed faster in MC (perilla + sorghum + sesame + proso millet + foxtail millet), with minimal nymphal mortality, higher adult emergence, longer adult longevity and heavier adult weight. The positive adult bug’s performance on the MC (perilla + sorghum + sesame + proso millet + foxtail millet) may be due to the nutrient contents and physicochemical components associated with the crop seeds. Further, the findings of this study are discussed in terms of bug’s behavioral response to crop seed species combinations and management strategies of Nysius sp. as an alternative to chemical management.

Olfactory Response and Feeding Preference of Liriomyza huidobrensis (Diptera: Agromyzidae) to Potato Cultivars

Maharjan, R.,Jung, C. Entomological Society of America 2016 Environmental entomology Vol.45 No.5

<P>Liriomyza huidobrensis (Blanchard) is a serious invasive pest of potato in Korea. In sessile insect herbivores like leafminer flies, host plant choice is a critical decision made by adults for offspring survival, and one that is often influenced by host plant quality. In annual host plants like potato, leafminer choice is sometimes influenced by cultivar characteristics. To determine the basis of host selection, the odor and feeding preferences of adult L. huidobrensis were examined using a five-arm olfactometer offering a choice of five different potato cultivars: 'Chubeak', 'Seohong', 'Goun', 'Dejima', and 'Sumi'. Preferences of adult leafminers varied significantly among potato cultivars. Liriomyza huidobrensis showed a preference for the Goun cultivar in both olfactory and adult feeding tests. When measured 2 wk after release onto actual plants, plant damage ratings and the number of mines were consistent with the feeding preference results. Further studies should examine the influence of cultivar characteristics on larval fitness and adult longevity to develop a resistant potato cultivar through the selection behavior of leafminers.</P>

In-situ synthesis of AgNPs in the natural/synthetic hybrid nanofibrous scaffolds: Fabrication, characterization and antimicrobial activities

Maharjan, Bikendra,Joshi, Mahesh Kumar,Tiwari, Arjun Prasad,Park, Chan Hee,Kim, Cheol Sang Elsevier 2017 Journal of the mechanical behavior of biomedical m Vol.65 No.-

<P><B>Abstract</B></P> <P>Silver nanoparticles embedded within a nanofibrous polymer matrix have significant attention in recent years as an antimicrobial wound dressing materials. Herein, we have fabricated a novel Ag-polyurethane-zein hybrid nanofibrous scaffold for wound dressing applications. AgNPs were synthesized in-situ via reduction of silver nitrate in electrospinning solution. Varying mass composition of the components showed the pronounced effect on the morphology and physicochemical properties of the composite fibers. Field-Emission Scanning Electron Microscopy (FESEM) images revealed that PU and zein with mass ratio 2:1 produced the bead-free continuous and uniformly distributed nanofibers. Fourier-transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and Thermogravimetric Analysis (TGA) confirmed the well interaction between component polymers. Compared to the pristine PU nanofibers, composite fibers showed enhanced tensile strength, young׳s modulus and surface wettability. The antibacterial capacity of the nanofibrous membrane was evaluated against gram-positive (<I>Staphylococcus aureus</I>) and gram-negative (<I>Escherichia coli</I>) bacterial strains via a zone of inhibition test, and the results showed high antibacterial performance for Ag incorporated composite mat. Experimental results of cell viability assay and microscopic imaging revealed that as-fabricated scaffolds have an excellent ability for fibroblast cell adhesion, proliferation and growth. Overall, as-fabricated antibacterial natural/synthetic composite scaffold can be a promising substrate for repairing skin defects.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel antibacterial natural/synthetic hybrid nanofibrous scaffold. </LI> <LI> Synthetization of AgNPs by in-situ reduction of silver nitrate in solution. </LI> <LI> Wettability and bioactivity of PU fiber improved by blending with biopolymer zein. </LI> <LI> Composite fibers showed enhanced tensile strength and young’s modulus. </LI> <LI> As-fabricated composite scaffold can be promising substrate for skin repairing. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Effect of proton-beam irradiation on cell survival of MCF-7 and its chemo-resistant subgroups

Maharjan Sushma,Lee Min-Gu,Nam Kyung-Soo,Lee Kyu-Shik 한국물리학회 2024 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.85 No.3

Proton beam (PB) therapy is gaining popularity for breast cancer treatment because of its enhanced coverage and potential to minimize late toxicities. The dose and volume of PB irradiation are closely associated with improved breast cancer survival. We aimed to investigate the impact of PB irradiation on the survival of the human breast cancer cells MCF-7 and their resistance to doxorubicin (MCF-7-DR) and paclitaxel (MCF-7-PR). Cells exposed to 0.5, 2, 4, or 8 Gy of PB irradiation showed a signifcant decrease in the survival of parent cells, even at 2 Gy, indicating therapeutic efcacy. Conversely, drug-resistant cells exhibited notable cytotoxicity at 4 and 8 Gy, which were above the daily recommended dose. Mechanistically, PB irradiation signifcantly altered the DNA repair proteins RAD51, Ku80, and survivin and cleaved PARP in MCF-7 cells compared to chemo-resistant cells, except for RAD51 and Ku80. In addition, cell-cycle regulators and MAPK expression were notably altered by PB irradiation compared to MCF-7-DR and PR cells, underscoring the importance of tailoring PB irradiation for enhanced efcacy against chemo-resistant breast cancer. These fndings suggest that PB irradiation downregulated RAD51 and Ku80, the potent DNA repair markers, underscoring its potential therapeutic efcacy in treating chemo-resistant breast cancer cells.

Comparison of Overall Immunity Levels among Workers at Grape Orchard, Rose Greenhouse, and Open-Field Onion Farm

Maharjan, Anju,Gautam, Ravi,Jo, JiHun,Acharya, Manju,Lee, DaEun,Pramod, Bahadur KC,Gim, Jin,Sin, Sojung,Kim, Hyocher,Kim, ChangYul,Lee, SooYeon,Lee, SooJin,Heo, Yong,Kim, HyoungAh Occupational Safety and Health Research Institute 2022 Safety and health at work Vol.13 No.2

Background: Occupational hazards in crop farms vary diversely based on different field operations as soil management, harvesting processes, pesticide, or fertilizer application. We aimed at evaluating the immunological status of crop farmers, as limited systematic investigations on immune alteration involved with crop farming have been reported yet. Methods: Immunological parameters including plasma immunoglobulin level, major peripheral immune cells distribution, and level of cytokine production from activated T cell were conducted. Nineteen grape orchard, 48 onion open-field, and 21 rose greenhouse farmers were participated. Results: Significantly low proportion of natural killer (NK) cell, a core cell for innate immunity, was revealed in the grape farmers (19.8±3.3%) in comparison to the onion farmers (26.4±3.1%) and the rose farmers (26.9±2.5%), whereas cytotoxic T lymphocyte proportion was lower in the grape and the onion farmers than the rose farmers. The proportion of NKT cell, an immune cell implicated with allergic response, was significantly higher in the grape (2.3±0.3%) and the onion (1.6±0.8%) farmers compared with the rose farmers (1.0±0.4%). A significantly decreased interferon-gamma:interleukin-13 ratio was observed from ex vivo stimulated peripheral blood mononuclear cells of grape farmers compared with the other two groups. The grape farmers revealed the lowest levels of plasma IgG1 and IgG4, and their plasma IgE level was not significantly different from that of the onion or the rose farmers. Conclusion: Our finding suggests the high vulnerability of workplace-mediated allergic immunity in grape orchard farmers followed by open-field onion farmers and then the rose greenhouse farmers.

Association of immunotoxicological indices with lung cancer biomarkers in poultry, grape, and rose farming workers

Maharjan Anju,Gautam Ravi,Acharya Manju,Jo JiHun,Lee DaEun,K C Pramod Bahadur,Lee Young-A,Kwon Jung-Taek,Kim HyoCher,Kim KyungRan,Kim ChangYul,Kim HyoungAh,Heo Yong 한국독성학회 2023 Toxicological Research Vol.39 No.4

Exposure to occupational hazards like dust, pesticides, diesel emission particles, or physical hazards in the agricultural sector is known to cause adverse health effects on farm workers. Our study aimed at addressing the association of immunomodulatory status with plasma levels of lung cancer biomarkers in farming population, attempting to recognition of vulnerable farming group. Blood samples from apparently healthy 51 chicken husbandry, 19 grape orchard, and 21 rose greenhouse workers were subjected to evaluate plasma levels of two representative lung cancer biomarkers, pro-gastrin releasing peptide (Pro-GRP) and cytokeratin fragment 19 (CYFRA 21-1). Peripheral blood mononuclear cells obtained from farmers were used for natural killer (NK) cell phenotyping and cytokines (interferon-gamma, IFN-γ and interleukin-13, IL-13) profiling in the culture supernatant. Compared to the rose greenhouse farmers, the grape orchard and chicken husbandry workers revealed a significantly upregulated plasma Pro-GRP and CYFRA 21-1 level. A low proportion of NK cells was observed among the female grape orchard workers and a lowered IFN- γ:IL-13 ratio was seen in the grape and chicken husbandry workers than the rose workers. Our findings imply that grape orchard and chicken husbandry workers have more disturbed immune homeostasis implicated with augmentation in the levels of lung cancer biomarkers than the rose greenhouse workers.

Sac-0601 prevents retinal vascular leakage in a mouse model of diabetic retinopathy

Maharjan, Sony,Lee, Sujin,Agrawal, Vijayendra,Choi, Hyun-Jung,Maeng, Yong-Sun,Kim, Kyeojin,Kim, Nam-Jung,Suh, Young-Ger,Kwon, Young-Guen Elsevier 2011 european journal of pharmacology Vol.657 No.1

<P><B>Abstract</B></P><P>Endothelium integrity is important for the normal functioning of vessels, the disruption of which can lead to disease. The blood-retinal barrier required for normal retinal function is compromised in diabetic retinopathy, causing retinal vascular leakage. Previously, we demonstrated the ability of Sac-0601[((2R,3S)-3-acetoxy-6-((3S,10R,13R,17R)-10,13-dimethyl-17-((R)-6-methylheptan-2-yl)-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-yloxy)-3,6-dihydro-2H-pyran-2-yl)methyl acetate], a pseudo-sugar derivative of cholesterol, to increase survival of retinal endothelial cells. In the present study, we evaluated the ability of Sac-0601 to prevent retinal vascular leakages <I>in vitro</I> and <I>in vivo</I>. Sac-0601 treatment blocked VEGF-induced formation of actin stress fibers and stabilized the cortical actin ring in retinal endothelial cells. It also inhibited degradation of occludin, an important tight junction protein, and blocked VEGF-induced disruption of its linear pattern at the cell border. The [<SUP>14</SUP>C] sucrose permeability assay demonstrated that Sac-0601 was able to prevent VEGF-induced retinal endothelial permeability. The compound inhibited the vascular leakage in retina of mice intravitreally injected with VEGF. And it also significantly reduced the leakage in retina of diabetic retinopathy mice model. Taken together, our findings suggest the potential therapeutic usefulness of Sac-0601 for retinal vascular permeability diseases.</P>