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Generation of Charged SiC Nanoparticles During HWCVD Process
Daseul Kim,Du Yun Kim,Ji Hye Kwon,Kun‑Su Kim,Nong‑Moon Hwang 대한금속·재료학회 2020 ELECTRONIC MATERIALS LETTERS Vol.16 No.5
Charged nanoparticles have been shown to be spontaneously generated in the gas phase in various chemical vapor deposition(CVD). Furthermore, it has been shown that these charged nanoparticles can contribute to the growth of thin films,nanowires, nanotetrapods and so on. Here, the generation of charged silicon carbide (SiC) nanoparticles in the gas phaseduring a hot wire CVD process was studied by capturing nanoparticles with a different delay time on a silicon monoxidemembrane of the copper mesh grid for transmission electron microscope. The average size of SiC nanoparticles capturedfor 30 s increased from 2.9 to 6.1 nm with increasing delay time from 0 to 60 min. The deposition behavior of SiC filmswas affected by the applied bias on a substrate holder. A homo-epitaxial SiC film as thick as ~ 200 nm was grown under thesubstrate bias of − 200 V, whereas polycrystalline SiC films were grown under 0 V and + 15 V. It indicates that nanoparticlesgenerated in the gas phase should be charged.
골격 좌표 벡터 및 LSTM 모델을 이용한 넘어짐 검출
시종욱(Jongwook Si),손현철(Hyeoncheol Son),김다슬(Daseul Kim),김문년(Moonnyeon Kim),정지연(Jiyeon Jeong),김규리(Gyuree Kim),김영형(Younghyung Kim),김성영(Sungyoung Kim) 한국정보기술학회 2020 한국정보기술학회논문지 Vol.18 No.12
In recent years, many countries around the world have faced the problems of aging, single-person households, and the increasing number of elderly people who live alone. These problems have led to the need for research to improve the quality of life for the elder and single-person households. In this paper, the method of detecting falls was proposed with a focus on the aspect of safety among the methods of improving the quality of life of single-person households. First, we extract key points of human skeletons based on the existing method, vectorize them to represent correlation between them, and input them into LSTM to determine whether falls have occurred. We try to enhance the performance by using only representative feature points, not all feature points. Five reference datasets were used to evaluate the performance of proposed system and performed well in most datasets.
Dictyopteris undulata Extract Induces Apoptosis in Human Colon Cancer Cells
Kim, Areum Daseul,Kang, Kyoung Ah,Piao, Jing Mei,Kim, Ki Cheon,Zheng, Jian,Yao, Cheng Wen,Cha, Ji Won,Hyun, Chang Lim,Boo, Sun Jin,Lee, Nam Ho,Na, Soo Young,Hyun, Jin Won 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.3
The present study investigated the cytotoxic and apoptotic effects of an ethanol extract derived from the marine brown alga Dictyopteris undulata against human colon adenocarcinoma cells. The Dictyopteris undulata extract (DUE) showed cytotoxic activity against SW480 cells in a dose-dependent manner, with 50% inhibition of cell viability at a concentration of $40{\mu}g/mL$. DUE also induced programmed cell death in SW480 cells, as evidenced by apoptotic body formation, DNA fragmentation, an increase in the population of apoptotic sub-$G_1$ phase cells, and mitochondrial membrane depolarization. Moreover, DUE significantly modulated the expression of apoptosis-associated proteins, resulting in a decrease in B cell lymphoma-2 expression and an increase in Bcl-2-associated X protein expression, as well as the activation of caspase-9 and caspase-3. Furthermore, DUE showed apoptotic cell death in two other colon cancer cell lines, SNU407 and HT29. These observations suggest that DUE may prove useful as a therapeutic agent for the attenuation of colon cancer.
( Daseul Hwang ),( Hyung Won Ryu ),( Ji-won Park ),( Jung-hee Kim ),( Doo-young Kim ),( Jae-hoon Oh ),( Ok-kyoung Kwon ),( Sang-bae Han ),( Kyung-seop Ahn ) 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.1
Peucedanum japonicum Thunberg (PJT) has been used in traditional medicine to treat colds, coughs, fevers, and other inflammatory diseases. The goal of this study was to investigate whether 3′-isovaleryl-4′-senecioylkhellactone (IVSK) from PJT has anti-inflammatory effects on lung epithelial cells. The anti-inflammatory effects of IVSK were evaluated using phorbol 12-myristate 13-acetate (PMA)-stimulated A549 cells and regular human lung epithelial cells as a reference. IVSK reduced the secretion of the inflammatory mediators interleukin (IL)-8 and monocyte chemoattractant protein-1 (MCP-1), and the mRNA expression of IL-6, IL-8, MCP-1, and IL-1β. Additionally, it inhibited the phosphorylation of IκB kinase (IKK), p65, Iκ-Bα, and mitogen-activated protein kinases (MAPKs) p38, JNK, and ERK in A549 cells stimulated with PMA. Moreover, the binding affinity of activator protein-1 (AP-1) and nuclear factor-κB (NF-κB) was significantly reduced in the luciferase assay, while nuclear translocation was markedly inhibited by IVSK in the immunocytochemistry. These findings indicate that IVSK can protect against inflammation through the AP-1 and NF-κB pathway and could possibly be used as a lead compound for the treatment of inflammatory lung diseases.
KIM, AREUM DASEUL,ZHANG, RUI,HAN, XIA,KANG, KYOUNG AH,PIAO, MEI JING,MAENG, YOUNG HEE,CHANG, WEON YOUNG,HYUN, JIN WON SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.12 No.3
<P>Reduced glutathione (GSH) is an abundant tripeptide present in the majority of cell types. GSH is highly reactive and is often conjugated to other molecules, via its sulfhydryl moiety. GSH is synthesized from glutamic acid, cysteine, and glycine via two sequential ATP?consuming steps, which are catalyzed by glutamate cysteine ligase (GCL) and GSH synthetase (GSS). However, the role of GSH in cancer remains to be elucidated. The present study aimed to determine the levels of GSH and GSH synthetic enzymes in human colorectal cancer. The mRNA and protein expression levels of GSH, the catalytic subunit of GCL (GCLC) and GSS were significantly higher in the following five colon cancer cell lines: Caco?2, SNU?407, SNU?1033, HCT?116, and HT?29, as compared with the normal colon cell line, FHC. Similarly, in 9 out of 15 patients with colon cancer, GSH expression levels were higher in tumor tissue, as compared with adjacent normal tissue. In addition, the protein expression levels of GCLC and GSS were higher in the tumor tissue of 8 out of 15, and 10 out of 15 patients with colon cancer respectively, as compared with adjacent normal tissue. Immunohistochemical analyses confirmed that GCLC and GSS were expressed at higher levels in colon cancer tissue, as compared with normal mucosa. Since GSH and GSH metabolizing enzymes are present at elevated levels in colonic tumors, they may serve as clinically useful biomarkers of colon cancer, and/or targets for anti-colon cancer drugs.</P>
Kim, Daseul,Chang, Hun Soo,Won, Eunsoo,Ham, Byung-Joo,Lee, Min-Soo The Korean Society of Biological Psychiatry 2016 생물정신의학 Vol.23 No.4
Objectives To determine the relationship between the Alu insertion/deletion (I/D) polymorphism in the tissue-type plasminogen activator (tPA) gene and the clinical outcome of mirtazapine treatment in Korean major depressive disorder (MDD) patients. Methods We enrolled 422 patients in this study. Symptoms were evaluated using the 21-item Hamilton Depression Rating (HAMD-21) Scale. After 1, 2, 4, and 8 weeks of mirtazapine treatment, the association between the Alu I/D polymorphism in the tPA gene and remission/response outcomes were evaluated. Results The proportion of I/I homozygotes in responders was higher than that in non-responders, whereas the proportion of D/D homozygotes in responders was lower than that in non-responders at 8 weeks of treatment (p = 0.032, OR = 1.57). The percentage decline of HAMD-21 scores in I allele carriers was larger than that of D/D homozygotes at 2 and 8 weeks of treatment (p = 0.035 and 0.007, respectively). I allele carriers were associated with remission at 8 weeks of treatment (p = 0.047, OR = 2.2). Conclusions These results show that treatment response and remission to mirtazapine were associated with the Alu I/D polymorphism of the tPA gene. This suggests the Alu I/D polymorphism may be a potential genetic marker for the prediction of therapeutic response to mirtazapine treatment in patients with MDD.
Kim, Areum Daseul,Zhang, Rui,Kang, Kyoung Ah,You, Ho Jin,Hyun, Jin Won Molecular Diversity Preservation International (MD 2011 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.12 No.12
<P>Jeju ground water, containing vanadium compounds, was shown to increase glutathione (GSH) levels as determined by a colorimetric assay and confocal microscopy. To investigate whether the effects of Jeju ground water on GSH were specifically mediated by vanadium compounds, human Chang liver cells were incubated for 10 passages in media containing deionized distilled water (DDW), Jeju ground water (S1 and S3), and vanadyl sulfate (VOSO<SUB>4</SUB>). Vanadyl sulfate scavenged superoxide anion, hydroxyl radical and intracellular reactive oxygen species. Vanadyl sulfate effectively increased cellular GSH level and up-regulated mRNA and protein expression of a catalytic subunit of glutamate cysteine ligase (GCLC), which is involved in GSH synthesis. The induction of GCLC expression by vanadyl sulfate was found to be mediated by transcription factor erythroid transcription factor NF-E2 (Nrf2), which critically regulates GCLC by binding to the antioxidant response elements (AREs). Vanadyl sulfate treatment increased the nuclear translocation of Nrf2 and the accumulation of phosphorylated Nrf2. Extracellular regulated kinase (ERK) contributed to ARE-driven GCLC expression via Nrf2 activation. Vanadyl sulfate induced the expression of the active phospho form of ERK. Taken together, these results suggest that the increase in GSH level by Jeju ground water is, at least in part, due to the effects of vanadyl sulfate via the Nrf2-mediated induction of GCLC.</P>
Kim, Nayoung,Cho, Daseul,Kim, Hyunjin,Kim, Sujin,Cha, Young‐,je,Greulich, Heidi,Bass, Adam,Cho, Hyun‐,Soo,Cho, Jeonghee Alan R. Liss, Inc 2020 International journal of cancer Vol.146 No.8
<P>Somatic mutations of epidermal growth factor receptor (EGFR) occur in ~3% of colorectal cancer (CRC) patients. Here, through systematic functional screening of 21 recurrent EGFR mutations selected from public data sets, we show that 11 colon cancer‐derived EGFR mutants (G63R, E114K, R165Q, R222C, S492R, P596L, K708R, E709K, G719S, G724S and L858R) are oncogenic and able to transform cells in a ligand‐independent manner. We demonstrate that cellular transformation by these mutants requires receptor dimerization. Importantly, the EGF‐induced and constitutive oncogenic potential of these EGFR mutants are inhibited by cetuximab or panitumumab <I>in vivo</I> and <I>in vitro</I>. Taken together, we propose that a subset of EGFR mutations can serve as genomic predictors for response to anti‐EGFR antibodies and that metastatic CRC patients with such mutations may benefit from these drugs as part of the first‐line therapy.</P>