다양한 농작업 환경 미세먼지 노출관련 농작업자 면역력 변화

Maharjan Anju(Maharjan Anju ),Acharya Manju(Acharya Manju ),DaEun Lee(DaEun Lee),K.C. Pramod Bahadur(K.C. Pramod Bahadur ),Kusma Sarina(Kusma Sarina ),Adhikari Manisha(Adhikari Manisha ),ChangYul Kim( 한국환경보건학회 2022 한국환경보건학회 학술대회 자료집 Vol.2022 No.5

Evaluation of epithelial transport and oxidative stress protection of nanoengineered curcumin derivative-cyclodextrin formulation for ocular delivery

Pooja Maharjan,Minki Jin,Daseul Kim,JaeWook Yang,Anjila Maharjan,Meong Cheol Shin,Kwan Hyung Cho,김만수,Kyoung Ah Min 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.10

Ocular drug delivery has been a well-knownroute for the drug administration for the treatment of oculardiseases. However, numerous anatomical and physiologicalbarriers prevailing in the eye itself create considerablechallenges for achieving the necessitated therapeutic efficacyalong with ocular bioavailability. However, recentadvances in nanoengineered strategies hold definite promisesin terms of devising improved ophthalmic medicinesfor the effective drug delivery to target the sites withenhanced ocular bioavailability. Curcumin, a hydrophobicpolyphenol yellow colored compound, and its metabolicreduced product, tetrahydrocurcumin (THC), have beenknown for their beneficial pharmacological functions, suchas anti-inflammatory or anti-oxidant activities at varioustissue sites. However, the low aqueous solubility of thesecompounds results in their poor bioavailability, therebylimiting their widespread application. Therefore, in thepresent study, we investigated the changes in drug solubilityby forming inclusion complexes with differentderivatives of hydroxypropyl (HP)-cyclodextrins (CD). To this end, the spray drying technique was used for nanoengineeringcurcumin or THC-loaded formulations toimprove the stability of formulations during the storage. The formulations were characterized in terms of physicochemicalproperties and cellular permeability. The resultsdemonstrated that the encapsulation of curcumin (or THC)into the HP-CDs significantly increased the drug solubilityand enhanced the corneal and retinal epithelial permeability. Curcumin or THC complexes in HP-CDs withimproved bioavailability also induced anti-oxidant activity(SOD1, CAT1, and HMOX1) in higher levels in the ocularepithelial cells and showed oxidative protection effects inrabbit cornea tissues that will boost up their application inocular medicine.

Sac-0601 prevents retinal vascular leakage in a mouse model of diabetic retinopathy

Maharjan, Sony,Lee, Sujin,Agrawal, Vijayendra,Choi, Hyun-Jung,Maeng, Yong-Sun,Kim, Kyeojin,Kim, Nam-Jung,Suh, Young-Ger,Kwon, Young-Guen Elsevier 2011 european journal of pharmacology Vol.657 No.1

<P><B>Abstract</B></P><P>Endothelium integrity is important for the normal functioning of vessels, the disruption of which can lead to disease. The blood-retinal barrier required for normal retinal function is compromised in diabetic retinopathy, causing retinal vascular leakage. Previously, we demonstrated the ability of Sac-0601[((2R,3S)-3-acetoxy-6-((3S,10R,13R,17R)-10,13-dimethyl-17-((R)-6-methylheptan-2-yl)-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-yloxy)-3,6-dihydro-2H-pyran-2-yl)methyl acetate], a pseudo-sugar derivative of cholesterol, to increase survival of retinal endothelial cells. In the present study, we evaluated the ability of Sac-0601 to prevent retinal vascular leakages <I>in vitro</I> and <I>in vivo</I>. Sac-0601 treatment blocked VEGF-induced formation of actin stress fibers and stabilized the cortical actin ring in retinal endothelial cells. It also inhibited degradation of occludin, an important tight junction protein, and blocked VEGF-induced disruption of its linear pattern at the cell border. The [<SUP>14</SUP>C] sucrose permeability assay demonstrated that Sac-0601 was able to prevent VEGF-induced retinal endothelial permeability. The compound inhibited the vascular leakage in retina of mice intravitreally injected with VEGF. And it also significantly reduced the leakage in retina of diabetic retinopathy mice model. Taken together, our findings suggest the potential therapeutic usefulness of Sac-0601 for retinal vascular permeability diseases.</P>

Microstrip Bandpass Filters Using Window Hairpin Resonator and T-Feeder Coupling Lines

Maharjan, R. K.,Kim, N. Y. KING FAHD UNIVERSITY OF PETROLEUM MINERALS 2014 Arabian journal for science and engineering: AJSE Vol.39 No.5

A new structure of planar window hairpin-based bandpass filters with symmetrical T-shaped feeder coupling line resonators is introduced. With varying different width in X axis and length in Y axis of the window of the hairpin resonator configuration, correspondingly the changes can be observed in the resonant frequency and useable bandwidth. By these methods, frequency tuning can be easily achieved by adjusting hairpin window dimension of the structure. Both filters were designed for 5.205 GHz resonant frequency. The geometrical window hairpin structure areas of both type filters are quite similar and both filters are resonated at almost the same frequency. The measurement results show that return loss (S-11) for both filters is higher than 23.0 dB and insertion loss (S-21) is measured less than -1.4 dB at resonant frequency of 5.13 GHz. The S-parameter responses of the fabricated filter nearly match with the electromagnetic simulated results; therefore, the feasibility of practical application of the proposed filters can be expected.

Prevalence of Puccinia abrupta var. partheniicola and its impact on Parthenium hysterophorus in Kathmandu Valley, Nepal

Maharjan, Seerjana,Devkota, Anjana,Shrestha, Bharat Babu,Baniya, Chitra Bahadur,Rangaswamy, Muniappan,Jha, ramod Kumar The Ecological Society of Korea 2020 Journal of Ecology and Environment Vol.44 No.4

Background: Parthenium hysterophorus is a noxious invasive weed in tropical and subtropical regions of the world, including Nepal. Among 11 species of biological control agents released to control P. hysterophorus in Ausrtalia, winter rust Puccina abrupta var. partheniicola arrived fortuitously and has established in Kathmandu Valley, Nepal, nearly a decade back. However, the prevalence and effectiveness of this rust as a biological control agent in Kathmandu remain unknown. To address this knowledge gap, a roadside survey was done at an interval of 2 ± 0.25 km in Kathmandu Valley to assess the P. abrupta var. partheniicola incidence and its impacts on P. hysterophorus. Infested individuals of P. hysterophorus were further divided into four severity classes (very low, low, medium, and high), and rust incidence was calculated. The impact of the winter rust on the growth of P. hysterophorus was assessed by comparing biomass and seed output of infested and non-infested individuals. Results: Among 81 locations where P. hysterophorus was present in the Kathmandu Valley, winter rust infestation was observed at 98% locations. At some locations within Kathmandu Valley such as Tinkune, Kirtipur, Chabahil, Buddha Chowk, and Dhobighat, the impacts of the rust on P. hysterophorus were medium to high. Aboveground biomass and seed output of P. hysterophorus were reduced by 47% and 73%, respectively, due to winter rust infestation. The study indicates that winter rust incidence is widespread in Kathmandu Valley with very low to high levels of damages to P. hysterophorus depending on the localities. Conclusion: The rust has, therefore, a potential to reduce the growth performance of P. hysterophorus, and it can be used as a component of integrated management of P. hysterophorus by introducing to other suitable areas in Nepal.

Microstrip Cross-coupled Interdigital SIR Based Bandpass Filter

Maharjan, R.K.,Shrestha, B.,Kim, N.Y. Radioengineering Society 2012 Radioengineering Vol.21 No.3

Olfactory Response and Feeding Preference of Liriomyza huidobrensis (Diptera: Agromyzidae) to Potato Cultivars

Maharjan, R.,Jung, C. Entomological Society of America 2016 Environmental entomology Vol.45 No.5

<P>Liriomyza huidobrensis (Blanchard) is a serious invasive pest of potato in Korea. In sessile insect herbivores like leafminer flies, host plant choice is a critical decision made by adults for offspring survival, and one that is often influenced by host plant quality. In annual host plants like potato, leafminer choice is sometimes influenced by cultivar characteristics. To determine the basis of host selection, the odor and feeding preferences of adult L. huidobrensis were examined using a five-arm olfactometer offering a choice of five different potato cultivars: 'Chubeak', 'Seohong', 'Goun', 'Dejima', and 'Sumi'. Preferences of adult leafminers varied significantly among potato cultivars. Liriomyza huidobrensis showed a preference for the Goun cultivar in both olfactory and adult feeding tests. When measured 2 wk after release onto actual plants, plant damage ratings and the number of mines were consistent with the feeding preference results. Further studies should examine the influence of cultivar characteristics on larval fitness and adult longevity to develop a resistant potato cultivar through the selection behavior of leafminers.</P>

Symmetrical Spiral Inductor-Coupled Bandpass Filter with a Stepped-Down Metal Width

Maharjan, R. K.,Kim, N. Y. KING FAHD UNIVERSITY OF PETROLEUM MINERALS 2014 Arabian journal for science and engineering: AJSE Vol.39 No.3

A compact microstrip symmetrical rectangular spiral inductor-coupled bandpass filter using a variable stepped-down metal width is presented. The proposed filter is based on an electromagnetic coupling between two symmetrical spiral turns with variable metal widths and spacing. The asymmetrical port terminals are coupled to the main inductor-resonators as a new coupling scheme using two narrow tuning stubs. The coupling scheme of the ports with the stubs maintains the 50 Omega impedance matching with input and output devices. The two symmetrical spiral inductors in the design structure are precisely mounted and adjusted together with equal spacing and gaps, so that the combined configuration behaves as a single resonator as inductor-interdigital coupling. The structure of combining two such resonators enables strong inductive coupling and effective capacitive coupling between the two ports and generates a resonant frequency of 5.597 GHz. The measured insertion loss (S (21)) was -0.93 dB, and the measured return loss (S (11)) was -24.4 dB.

In-situ synthesis of AgNPs in the natural/synthetic hybrid nanofibrous scaffolds: Fabrication, characterization and antimicrobial activities

Maharjan, Bikendra,Joshi, Mahesh Kumar,Tiwari, Arjun Prasad,Park, Chan Hee,Kim, Cheol Sang Elsevier 2017 Journal of the mechanical behavior of biomedical m Vol.65 No.-

<P><B>Abstract</B></P> <P>Silver nanoparticles embedded within a nanofibrous polymer matrix have significant attention in recent years as an antimicrobial wound dressing materials. Herein, we have fabricated a novel Ag-polyurethane-zein hybrid nanofibrous scaffold for wound dressing applications. AgNPs were synthesized in-situ via reduction of silver nitrate in electrospinning solution. Varying mass composition of the components showed the pronounced effect on the morphology and physicochemical properties of the composite fibers. Field-Emission Scanning Electron Microscopy (FESEM) images revealed that PU and zein with mass ratio 2:1 produced the bead-free continuous and uniformly distributed nanofibers. Fourier-transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and Thermogravimetric Analysis (TGA) confirmed the well interaction between component polymers. Compared to the pristine PU nanofibers, composite fibers showed enhanced tensile strength, young׳s modulus and surface wettability. The antibacterial capacity of the nanofibrous membrane was evaluated against gram-positive (<I>Staphylococcus aureus</I>) and gram-negative (<I>Escherichia coli</I>) bacterial strains via a zone of inhibition test, and the results showed high antibacterial performance for Ag incorporated composite mat. Experimental results of cell viability assay and microscopic imaging revealed that as-fabricated scaffolds have an excellent ability for fibroblast cell adhesion, proliferation and growth. Overall, as-fabricated antibacterial natural/synthetic composite scaffold can be a promising substrate for repairing skin defects.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel antibacterial natural/synthetic hybrid nanofibrous scaffold. </LI> <LI> Synthetization of AgNPs by in-situ reduction of silver nitrate in solution. </LI> <LI> Wettability and bioactivity of PU fiber improved by blending with biopolymer zein. </LI> <LI> Composite fibers showed enhanced tensile strength and young’s modulus. </LI> <LI> As-fabricated composite scaffold can be promising substrate for skin repairing. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Gomisin G Inhibits the Growth of Triple-Negative Breast Cancer Cells by Suppressing AKT Phosphorylation and Decreasing Cyclin D1

Maharjan, Sony,Park, Byoung Kwon,Lee, Su In,Lim, Yoonho,Lee, Keunwook,Kwon, Hyung-Joo The Korean Society of Applied Pharmacology 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.3

A type of breast cancer with a defect in three molecular markers such as the estrogen receptor, progesterone receptor, and human epidermal growth factor receptor is called triple-negative breast cancer (TNBC). Many patients with TNBC have a lower survival rate than patients with other types due to a poor prognosis. In this study, we confirmed the anti-cancer effect of a natural compound, Gomisin G, in TNBC cancer cells. Treatment with Gomisin G suppressed the viability of two TNBC cell lines, MDA-MB-231 and MDA-MB-468 but not non-TNBC cell lines such as MCF-7, T47D, and ZR75-1. To investigate the molecular mechanism of this activity, we examined the signal transduction pathways after treatment with Gomisin G in MDA-MB-231 cells. Gomisin G did not induce apoptosis but drastically inhibited AKT phosphorylation and reduced the amount of retinoblastoma tumor suppressor protein (Rb) and phosphorylated Rb. Gomisin G induced in a proteasome-dependent manner a decrease in Cyclin D1. Consequently, Gomisin G causes cell cycle arrest in the G1 phase. In contrast, there was no significant change in T47D cells except for a mild decrease in AKT phosphorylation. These results show that Gomisin G has an anti-cancer activity by suppressing proliferation rather than inducing apoptosis in TNBC cells. Our study suggests that Gomisin G could be used as a therapeutic agent in the treatment of TNBC patients.