Microneedle patch delivery to the skin of virus-like particles containing heterologous M2e extracellular domains of influenza virus induces broad heterosubtypic cross-protection

Kim, M.C.,Lee, J.W.,Choi, H.J.,Lee, Y.N.,Hwang, H.S.,Lee, J.,Kim, C.,Lee, J.S.,Montemagno, C.,Prausnitz, M.R.,Kang, S.M. Elsevier Science Publishers 2015 Journal of controlled release Vol.210 No.-

A broadly cross-protective influenza vaccine that can be administrated by a painless self-immunization method would be a value as a potential universal mass vaccination strategy. This study developed a minimally-invasive microneedle (MN) patch for skin vaccination with virus-like particles containing influenza virus heterologous M2 extracellular (M2e) domains (M2e5x VLPs) as a universal vaccine candidate without adjuvants. The stability of M2e5x VLP-coated microneedles was maintained for 8weeks at room temperature without losing M2e antigenicity and immunogenicity. MN skin immunization induced strong humoral and mucosal M2e antibody responses and conferred cross-protection against heterosubtypic H1N1, H3N2, and H5N1 influenza virus challenges. In addition, M2e5x VLP MN skin vaccination induced T-helper type 1 responses such as IgG2a isotype antibodies and IFN-γ producing cells at higher levels than those by conventional intramuscular injection. These potential immunological and logistic advantages for skin delivery of M2e5x VLP MN vaccines could offer a promising approach to develop an easy-to-administer universal influenza vaccine.

Study on the improved J_c(B) performance of polyacrylic acid-doped MgB₂ bulks

Hwang, S.M.,Lee, C.M.,Lee, S.M.,Sung, K.,Joo, J.,Lim, J.H.,Kang, W.N.,Kim, C.J. North-Holland 2010 Physica. C, Superconductivity Vol.470 No.suppl1

We fabricated polyacrylic acid (PAA)-doped MgB<SUB>2</SUB> bulks by wet mixing and in situ reaction process and studied the relationships between the phase formation, lattice change, microstructure, and critical properties according to the PAA doping level. The PAA doping increased the MgO amount and grain boundary area but decreased the a-axis lattice constant and critical temperature (T<SUB>c</SUB>), which is indicative of the substitution of B sites by C in MgB<SUB>2</SUB>. Furthermore, the critical current density (J<SUB>c</SUB>) at high magnetic fields improved with the increasing PAA content to 5.99kA/cm<SUP>2</SUP> for the 7wt% PAA-doped sample, which was about 6-fold higher than that of the pure sample at 5K and 6.6T. This improvement was mainly attributed to the C substitution and the fine grain size due to PAA doping, suggesting that PAA is an effective dopant in improving J<SUB>c</SUB>(B) performance of MgB<SUB>2</SUB>.

M-13 bacteriophage based structural color sensor for detecting antibiotics

Moon, J.S.,Park, M.,Kim, W.G.,Kim, C.,Hwang, J.,Seol, D.,Kim, C.S.,Sohn, J.R.,Chung, H.,Oh, J.W. Elsevier Sequoia 2017 Sensors and actuators. B Chemical Vol.240 No.-

Color sensor systems that exploit the advantages of M-13 bacteriophage have been shown to be potentially useful for detection of hazardous materials. The properties of M-13 bacteriophage can be systemically modified to impart target-specific selectivity and sensitivity using the phage display technique. Here, we describe a structural color-based sensor that utilizes genetically engineered M-13 bacteriophage to discriminate different types of antibiotics. An M-13 bacteriophage based structural color matrix was fabricated using a simple pulling technique by self-assembly of M-13 bacteriophage. When exposed to organic solvent, M-13 bacteriophage bundles promptly swell and promote distinct structural color change. Color sensors composed of M-13 bacteriophage genetically engineered to possess WHW peptide motifs clearly discriminated three different types of antibiotics, which was based on the color analysis of sensor using principal component analysis. Our sensing approach based on M-13 bacteriophage could be a promising sensor technique such as an environmental monitoring system.

Characterization of Nutritional Value for Twenty-one Pork Muscles

Kim, J.H.,Seong, P.N.,Cho, S.H.,Park, B.Y.,Hah, K.H.,Yu, L. H.,Lim, D.G.,Hwang, I.H.,Kim, D.H.,Lee, J.M.,Ahn, C.N. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.1

A study was conducted to evaluate nutritional value for twenty-one pork muscles. Ten market-weight crossbred pigs (five gilts and five barrows) were used for evaluating proximate chemical composition, cholesterol, total iron, calorie and fatty acid contents. As preliminary analysis revealed no noticeable sex effect, pooled data from both sexes were used for the final analysis. M. rectus femoris had the highest moisture content, while m. latissimus dorsi was lowest in moisture content (p<0.05). Protein content was highest for m. longissimus dorsi and lowest for m. supraspinatus (p<0.05). The tensor fasciae and latissimus dorsi muscles contained the highest intramuscular fat (p<0.05), while rectus femoris, adductor and vastus lateralis were lowest in intramuscular fat content. When simple correlations between chemical values were computed for the pooled dataset from all muscles, intramuscular fat had significant (p<0.05) negative linear relationships with moisture (r = -0.85) and protein (r = -0.51) contents. Calorie levels were not significantly affected by fat content, while rectus femoris and latissimus dorsi muscles showed lowest and highest calorie contents, respectively (p<0.05). Polyunsaturated fatty acid content was highest (p<0.05) for both m. adductor and m. rectus femoris, while it was lowest for m. longissimus dorsi. Collectively, the current study identified a large amount of variation in nutritional characteristics between pork muscles, and the data can be used for the development of muscle-specific strategies to improve eating quality of meats and meat products.

논문 : 생물생산시설 및 환경공학 ; 소규모 복도-더그매 예열 음압환기방식 무창자돈사의 최적 환기 요건에 관한 연구

이승주 ( S. J. Lee ),장동일 ( D. I. Chang ),황선호 ( S. H. Hwang ),( W. M. Gutierrez ),장홍희 ( H. H. Chang ) 한국농업기계학회 2010 바이오시스템공학 Vol.35 No.6

This study was carried out to determine necessary conditions for optimal ventilation of small windowless piglet house (4.0 (W) × 11.0 (L) × 2.6(H) m) with corridor and attic for preheating using CFD (Computational Fluid Dynamics) simulation. The experimental weaning piglet house was consisted of a corridor, an attic, 4 rooms (3.0 (W) × 2.75(L) m), 3 fences (0.7(H) m), 5 air inlets and 2 exhaust fans (0.4 (D) m) and simulated using CFD code, FLUENT. The simulation results for the experimental weaning piglet house showed that each room was uniformly ventilated under all the experimental conditions and air velocities at 0.1 m above floor are less than 0.15 m/s for 0.75 m/s and 1.0 m/s of air inlet velocity but 0.61 m/s for 1.25 m/s. The simulation results are similar to the measured results. Considering the air flow pattern, ventilating efficiency, air velocity at 0.1 m above floor and cold stress of weaning piglets and so on, the optimum velocity of air inlet might be 1.0 m/s.

Effects of catalpalactone on dopamine biosynthesis and L-DOPA-induced cytotoxicity in PC12 cells

Huang, H.S.,Han, X.H.,Hwang, B.Y.,Park, J.I.,Yoo, S.K.,Lee, H.J.,Lim, S.C.,Lee, M.K. Elsevier Science B.V 2008 Environmental toxicology and pharmacology Vol.26 No.1

The effects of catalpalactone on dopamine biosynthesis and L-DOPA-induced cytotoxicity in PC12 cells were investigated. Catalpalactone at 5-30μM decreased intracellular dopamine content with the IC<SUB>50</SUB> value of 22.1μM. Catalpalactone at 5-20μM, but not 30μM, did not alter cell viability. Catalpalactone at 20μM inhibited tyrosine hydroxylase (TH) and aromatic-l-amino acid decarboxylase (AADC) activities. Catalpalactone also decreased cyclic AMP levels and inhibited TH phosphorylation. In addition, catalpalactone at 20μM reduced the increases in dopamine levels induced by L-DOPA (20-50μM). Catalpalactone (5-30μM) associated with L-DOPA (50-100μM) enhanced L-DOPA-induced cytotoxicity at 48h, which was prevented by N-acetyl-l-cysteine. These results suggest that catalpalactone inhibited dopamine biosynthesis by reducing TH and AADC activities and enhanced L-DOPA-induced cytotoxiciy in PC12 cells.

A SUPER-JUPITER MICROLENS PLANET CHARACTERIZED BY HIGH-CADENCE KMTNET MICROLENSING SURVEY OBSERVATIONS OF OGLE-2015-BLG-0954

신인구,Y.-H. Ryu,A. Udalski,M. Albrow,S.-M. Cha,J.-Y. Choi,S.-J. Chung,C. Han,K.-H. Hwang,Y. K. Jung,D.-J. Kim,S.-L. Kim,C.-U. Lee,Y. Lee,B.-G. Park,H. Park,R. W. Pogge,J. C. Yee,P. Pietrukowicz,P. Mroz 한국천문학회 2016 Journal of The Korean Astronomical Society Vol.49 No.3

We report the characterization of a massive $(m_p=3.9\pm 1.4 M_{\rm jup})$ microlensing planet (OGLE-2015-BLG-0954Lb) orbiting an M dwarf host ($M=0.33\,\pm 0.12 M_\odot$) at a distance toward the Galactic bulge of $0.6^{+0.4}_{-0.2}\,$kpc, which is extremely nearby by microlensing standards. The planet-host projected separation is $a_\perp \sim 1.2\,\au$. The characterization was made possible by the wide-field ($4\,\rm deg^2$) high cadence ($\Gamma = 6\,\rm hr^{-1}$) monitoring of the Korea Microlensing Telescope Network (KMTNet), which had two of its three telescopes in commissioning operations at the time of the planetary anomaly. The source crossing time $t_*=16\,$min is among the shortest ever published. The high-cadence, wide-field observations that are the hallmark of KMTNet are the only way to routinely capture such short crossings. High-cadence resolution of short caustic crossings will preferentially lead to mass and distance measurements for the lens. This is because the short crossing time typically implies a nearby lens, which enables the measurement of additional effects (bright lens and/or microlens parallax). When combined with the measured crossing time, these effects can yield planet/host masses and distance.

세관 양광주 방전에서 플라즈마 확산의 완전 해

김동준,정종문,김정현,황하청,정재윤,조윤희,임현교,구제환,최은하,조광섭,Jin, D.J.,Jeong, J.M.,Kim, J.H.,Hwang, H.C.,Chung, J.Y.,Cho, Y.H.,Lim, H.K.,Koo, J.H.,Choi, E.H.,Cho, G.S. 한국진공학회 2010 Applied Science and Convergence Technology Vol.19 No.1

관경이 수 mm인 세관 램프 내부에서 플라즈마의 확산을 조사하기 위하여 이극성(ambipolar) 확산방정식을 해하였다. 반경 방향의 확산에 의한 유리관 벽에서의 플라즈마 소멸 특성시간은 $\tau_r\;=\;(r_0/2.4)^2/D_a$로 주어진다. 반경 $r_0{\sim}1\;mm$이고 이극성 확산계수 $D_a{\sim}0.01\;m^2/s$ 이면, $\tau_r{\sim}17\;{\mu}s$이다. 이는 램프의 교류전원 구동에서 플라즈마를 유지하기 위한 구동 최소 주파수 ~30 kHz에 해당한다. 고전압이 인가되는 전극부에 발생한 고밀도의 플라즈마가 양광주로 확산되는 특성시간은 $\tau_z{\sim}0.1\;s$이다. 고밀도 플라즈마 경계에서의 시간에 대한 확산속도는 $t{\sim}10^{-6}\;s$일 때 $u_D{\sim}10^2\;m/s$이고, $t{\sim}10^{-3}\;s$이면 그 속도는 $u_D{\sim}1\;m/s$로 느려진다. 따라서 램프 길이 ~1 m에 대하여 전극부에서 생성된 고밀도 플라즈마가 양광주 전체로 확산되는 시간은 수 초가 걸린다. The ambipolar diffusion equation has been solved in a fine-tube lamp of a few mm in diameter. In the diffusion of radial direction, the plasma diffuses and vanishes away at the glass wall by recombination with the characteristic time of plasma loss is given by $\tau_r\;=\;(r_0/2.4)^2/D_a$. With the radius $r_0{\sim}1\;mm$ and the ambipolar diffusion coefficient $D_a{\sim}0.01\;m^2/s$, the vanishing time is calculated $\tau_r{\sim}10\;{\mu}s$ which corresponds to the least value of frequency 30 kHz for the sustaining the plasma in the operation of high voltage AC-power. In the diffusion of longitudinal z-direction, a high density plasma generated at the area of a high voltage electrode, diffuses into the positive column with the characteristic time $\tau_z{\sim}0.1\;s$. The plasma diffusion velocity at the boundary of high density plasma is $u_D{\sim}10^2\;m/s$ at the time $t{\sim}10^{-6}$ s and the diffusion velocity becomes slow as $u_D{\sim}1\;m/s$ at $t{\sim}10^{-3}\;s$. Therefore, for the long lamp of 1 m, it takes about several seconds for the high density plasma at the area of electrode to diffuse through the whole positive column space.

Development of carbon-doped ex situ MgB₂ wire by mechanical alloying

Hwang, S.M.,Choi, J.H.,Park, E.C.,Lim, J.H.,Joo, J.,Kang, W.N.,Kim, C.J. North-Holland 2009 Physica. C, Superconductivity Vol.469 No.15

Carbon-doped ex situ MgB<SUB>2</SUB> wires were fabricated using a mechanical alloying (MA) method and the effect of the milling time on the phase formation, lattice change, and critical properties was investigated. The precursor powder was prepared with a mixture of MgB<SUB>2</SUB> and 1at% carbon black (CB) powder by mechanical alloying for 0-100h. The MA process effectively decreased the powder size and increased the structural disorder of the powders, while it did not provide the incorporation of C in the MgB<SUB>2</SUB> powders. On the other hand, C doping occurred successfully in the MA-treated wires after sintering. The addition of CB and the MA process improved the J<SUB>c</SUB>(B) and flux pinning force. These improvements are related to the combined effects of the smaller grains, more severe structural disorder, and higher level of CB doping by the MA process.

스피넬 결합 내화물에서의 부피 팽창 제어

황규홍,김종강,조명제,최정림,박정권 경상대학교 생산기술연구소 2001 工學硏究院論文集 Vol.1 No.-

To improve the mechanical and chemical properties of unfired A12O3-C refractories, which is widely used in metal line of steel-making ladle, MgO was added as a matrix powders and the degree of spinel formation was studied, Because the spinel was formed at the contact areas between A12O3 and MgO particles and the volume of in-situ formed spinel increased more abnormally at the side of A12O3 particles than MgO, A12O3 aggregates was more recommendable than MgO aggregates. So to compare the degree of spinel formation in the A12O3-MgO-C(AMC) refractories, two kinds of A12O3 aggregates was added in different content ratio and their effects on physical properties such as permanent volume expansion were examined. For A12O3 aggregates, low purity fused A12O3 (BFA) was more preferable than the high purity fused A12O3 (WFA) due to high density and the high degree of spinel formation. And corrosion resistance was increased with the amount of MgO added, and the finer the MgO powder, the better the residual expansion and minute structure formation was observed. But in case of a sudden increasement of spinel phase air gap would be appeared around aggregates and resulting crack showed deteriorative effect in anti-corrosion, so the appropriate quantity of MgO and it's grain size could be controlled for optimal residual expansion due to spinel formation and advancement of corrosion resistance by structural stability.