MicroRNA-576-3p Inhibits Proliferation in Bladder Cancer Cells by Targeting Cyclin D1

Liping Xie,Zhen Liang,Shiqi Li,Xin Xu,Xianglai Xu,Xiao Wang,Jian Wu,Yi Zhu,Zhenghui Hu,Yiwei Lin,Yeqing Mao,Hong Chen,Jindan Luo,Ben Liu,Xiangyi Zheng 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.2

MicroRNAs (miRNAs) are small, endogenous RNAs that play important gene-regulatory roles by binding to the imperfectly complementary sequences at the 3 -UTR of mRNAs and directing their gene expression. Here, we first discovered that miR-576-3p was down-regulated in human bladder cancer cell lines compared with the non-malignant cell line. To better characterize the role of miR-576-3p in bladder cancer cells, we over-expressed or down-regulated miR-576-3p in bladder cancer cells by transfecting with chemically synthesized mimic or inhibitor. The overexpression of miR-576-3p remarkably inhibited cell proliferation via G1-phase arrest, and decreased both mRNA and protein levels of cyclin D1 which played a key role in G1/S phase transition. The knock-down of miR-576-3p significantly promoted the proliferation of bladder cancer cells by accelerating the progression of cell cycle and increased the expression of cyclin D1. Moreover, the dual-luciferase reporter assays indicated that miR-576-3p could directly target cyclin D1 through binding its 3 -UTR. All the results demonstrated that miR-576-3p might be a novel suppressor of bladder cancer cell proliferation through targeting cyclin D1.

Effect of cohesive powders on pressure fluctuation characteristics of a binary gas-solid fluidized bed

Liping Wei,Youjun Lu,Jianbo Zhu,Guodong Jiang,Jun Hu,Haipeng Teng 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.10

The effect of cohesive particles on the pressure fluctuations was experimentally investigated in a binary gassolid fluidized bed. The pressure fluctuation signals were measured by differential pressure sensors under conditions of various weight percentages of cohesive particles. The cohesive particles increased the fixed bed pressure drop per unit height and decreased the minimum fluidization velocity. The Wen & Yu equation well predicts the minimum fluidization velocity of the binary system. The addition of cohesive particles slightly decreased the bubble size in bubbling flow regime when the cohesive particles and the coarse particles mixed well, while the bubble size greatly decreased when the cohesive particles agglomerated on the bed surface. The time series of pressure fluctuations was analyzed by using the methods of time domain, frequency domain and wavelet transformation. The normalized standard deviation of pressure fluctuations decreased with increasing weight percentages of cohesive particles. A wide bandwidth frequency of 0 to 1Hz got narrower with a single peak around 0.6Hz with an increase in proportion of the cohesive particles. The meso-energy and micro-energy of pressure fluctuations were decreasing with increasing cohesive particles proportions, which indicated that adding cohesive particles could reduce the energy dissipation of bubble and particle fluctuations.

Model Predictive Control based Discontinuous PWM Algorithm for 3L-NPC Inverter

Zhong Liping,Hu Song 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.1

This paper proposes a novel model predictive control based discontinuous PWM strategy for 3L-NPC inverter. Through the model predictive control principle and special output level sequence arrangement, the optimal voltage vector and zero sequence voltage are selected, so that the neutral point voltage can be controlled on the basis of discontinuous modulation strategy, thus achieve the purpose of balancing the neutral point voltage and reducing the switching loss. Because there are most three elements in the control set, the calculation of this method is quite simple. Compared with carrier based PWM and space vector PWM, it does not need vector synthesis and complex zero sequence voltage calculation, so it is easy to implement. Experimental results verify the validity of the method.

Fast modulation strategy for open-end winding PMSM with common DC bus

Zhong, Liping,Hu, Song The Korean Institute of Power Electronics 2021 JOURNAL OF POWER ELECTRONICS Vol.21 No.7

An open-end winding PMSM drive system with a common DC bus can produce zero-sequence current and high switching loss, which reduce the performance and efficiency of the system. Aiming at this problem, this paper proposes a fast modulation strategy that can effectively suppress the zero-sequence current, reduce the number of switching times in a sampling period to 6, and reduce the average switching loss by 50%. In addition, when compared with the conventional CBPWM and SVPWM algorithms, this strategy does not need to decompose the modulation wave, or synthesize the voltage vector. Furthermore, the calculation burden is small, which makes it very easy to implement. Experimental results verify the effectiveness and feasibility of this strategy.

Vps34 Inhibits Hepatocellular Carcinoma Invasion by Regulating Endosome-Lysosome Trafficking via Rab7-RILP and Rab11

Chenyang Qi,Liping Zou,Suxia Wang,Xing Mao,Yuan Hu,Jiaoyu Shi,Zhigang Zhang,Huijuan Wu 대한암학회 2022 Cancer Research and Treatment Vol.54 No.1

Purpose The role of vacuolar protein sorting 34 (Vps34), an indispensable protein required for cell vesicular trafficking, in the biological behavior of hepatocellular carcinoma (HCC) has yet to be studied. Materials and Methods In the present study, the expression of Vps34 in HCC and the effect of Vps34 on HCC cell invasion was detected both in vivo and in vitro. Furthermore, by modulating the RILP and Rab11, which regulate juxtanuclear lysosome aggregation and recycling endosome respectively, the underlying mechanism was investigated. Results Vps34 was significantly decreased in HCC and negatively correlated with the HCC invasiveness both in vivo and in vitro. Moreover, Vps34 could promote lysosomal juxtanuclear accumulation, reduce the invasive ability of HCC cells via the Rab7-RILP pathway. In addition, the deficiency of Vps34 in HCC cells affected the endosome-lysosome system, resulting in enhanced Rab11 mediated endocytic recycling of cell surface receptor and increased invasion of HCC cells. Conclusion Our study reveals that Vps34 acts as an invasion suppressor in HCC cells, and more importantly, the endosome-lysosome trafficking regulated by Vps34 has the potential to become a target pathway in HCC treatment.

MicroRNA-409-3p Inhibits Migration and Invasion of Bladder Cancer Cells via Targeting c-Met

Xin Xu,Liping Xie,Hong Chen,Yiwei Lin,Zhenghui Hu,Yeqing Mao,Jian Wu,Xianglai Xu,Yi Zhu,Shiqi Li,Xiangyi Zheng 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.1

There is increasing evidence suggesting that dysregulation of certain microRNAs (miRNAs) may con-tribute to tumor progression and metastasis. Previous studies have shown that miR-409-3p is dysregulated in some malignancies, but its role in bladder cancer is still unknown. Here, we find that miR-409-3p is down-regulated in human bladder cancer tissues and cell lines. Enforced expression of miR-409-3p in bladder cancer cells significantly reduced their migration and invasion without affecting cell viability. Bioinformatics analysis identified the pro-metastatic gene c-Met as a potential miR-409-3p target. Further studies indicated that miR-409-3p suppressed the expression of c-Met by binding to its 3-untranslated region. Silencing of c-Met by small interfering RNAs phenocopied the effects of miR-409-3p overexpression, whereas restoration of c-Met in bladder cancer cells bladder cancer cells overexpressing miR-409-3p, partially reversed the suppressive effects of miR-409-3p. We further showed that MMP2 and MMP9 may be downstream effector proteins of miR-409-3p. These findings indicate that miR-409-3p could be a potential tumor suppressor in bladder cancer.

Advanced Stability Analysis of the Tunnels in Jointed Rock Mass Based on TSP and DEM

Hongyun Fan,Liping Li,Hongliang Liu,Shaoshuai Shi,Jie Hu,Shen Zhou 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.4

The discrete element method (DEM) can analyze the large deformation and large displacement of rock mass effectively, and it is widely used in underground engineering, slope engineering and other fields. However, due to the low accuracy of rock mass structural surface information acquisition, the application of discrete element method in the analysis of jointed rock mass stability is still deviated. In this paper, combined with the advantages of the tunnel seismic prediction (TSP) in obtaining discontinuous geological interface information and the discrete element method in the calculation and analysis of jointed rock mass stability, this paper proposes an advanced analysis method for jointed rock mass stability based on TSP and DEM. Compared to the traditional methods, the analysis results of the jointed rock mass stability are more reliable. Firstly, relying on the advanced detection system – Tunnel Seismic Prediction 203Plus, the unstructured rock mass structure information of the tunnel is obtained, and the spatial attitude of the discontinuous geological interface is further determined. Secondly, based on the Fish programming language, the non-continuous geological interface information can be expressed in the discrete unit software – 3D Distinct Element Code (3DEC). In this way, the excavation calculation model of the tunnels in jointed rock mass can be constructed. Finally, based on the DEM, the excavation of the tunnels in jointed rock mass can be simulated, analyze the stability of surrounding rock during the tunnel excavation process, and realize the stability analysis of surrounding rock stability of jointed rock mass. Based on the Huangjiazhuang Tunnel Project, this paper uses the above method to carry out on-site application. The results show that the location of the dangerous block is predicted to be consistent with the actual exposure of the tunnel surrounding rock based on TSP and DEM, which verify the accuracy and feasibility of this method, and the research results have practical guiding significance for the safe construction of the tunnels in jointed rock mass.

Association between expression levels and growth trait-related SNPs located in promoters of the MC4R and MSTN genes in Spinibarbus hollandi

Yang Yang,Zhaojun Lan,Hu Shu,Huiqiang Zhou,Xiaolu Jiang,Liping Hou,Pinghua Gu 한국유전학회 2018 Genes & Genomics Vol.40 No.11

Melanocortin 4 receptor: (MC4R) and Myostatin (MSTN) are two important growth trait-related genes in animals. In this study, we showed that two SNPs, MC4R-719A>G and MSTN-519C>T, found in the promoters of the MC4R and MSTN genes, respectively, are both associated with growth traits in Spinibarbus hollandi. Furthermore, we observed that there were significant associations between the expression levels of the MC4R and MSTN genes and these two growth trait-related SNPs. The expression level of MC4R gene in brain was lower in GG genotype fish with extremely high growth performance than that in AA genotype fish with extremely low growth performance. Expression level of the MSTN gene in muscle was lower in TT genotype fish with extremely high growth performance than that in CC and CT genotype fish with lower growth performance. The results indicated that these SNPs located in the promoters of MC4R and MSTN are associated with growthrelated traits through modification of gene expression levels. The MSTN and MC4R SNPs may have useful application in effective marker-assisted selection aimed to increase output in S. hollandi.

Occurrence and Molecular Identification of Giardia duodenalis from Stray Cats in Guangzhou, Southern China

Guochao Zheng,Wei Hu,Yuanjia Liu,Qin Luo,Liping Tan,Guoqing Li 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.1

The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.

Low‐Voltage Pulsed Electric Field Sterilization on a Microfluidic Chip

Liu, Linlin,Zhao, Liping,Yang, Jun,Wan, Xiaoping,Hu, Ning,Yeh, Li‐,Hsien,Joo, Sang W.,Qian, Shizhi WILEY‐VCH Verlag 2013 Electroanalysis Vol.25 No.5

<P><B>Abstract</B></P><P>A polyimide substrate based microfluidic chip with thousands of comb‐shaped microelectrodes has been designed, fabricated, and tested for sterilization of bacteria by using pulsed electric field. The performance of bacteria sterilization as functions of the electric field strength, pulse number and width, treatment buffer, bacteria growth status, and bacteria enrichment by positive dielectrophoresis has been experimentally investigated on the microfluidic chip. Experimental results show that only 100 V are sufficient to obtain good sterilization of <I>Escherichia coli</I>. Higher electric field strength, bacteria enrichment by positive dielectrophoresis, longer pulse time, buffer with fewer components and nutritions, and suitable bacteria growth status also improve the sterilization of bacteria. In addition, configuration of the microelectrode array affects bacteria sterilization. This microfluidic device allows one to preconcentrate bacteria to a region with high electric field strength by using positive dielectrophoresis, and subsequently kill the enriched bacteria by applying a pulsed electric field through the same microelectrode array.</P>