재래돼지 미토콘드리아 게놈내 D-loop 영역의 염기서열 분석

김태헌,윤두학,이효신,정일정,조진기 ( T . H . Kim,D . H . Yoon,H . S . Lee,I . C . Cheong,J . K . Jo ) 한국축산학회 1997 한국축산학회지 Vol.39 No.3

The purpose of this study was to investigate differences in restriction fragment length polymorphisms between mitochondrial DNAs(mtDNA) of Korean native pig and Landrace and to construct restriction map of the mtDNA of Korean native pig. When the mtDNA were digested with 16 different restriction enzymes of Apa I, Ava I, Barnes I, Bgl I, Bgl II, Cla I, EcoR I, EcoRV, Hind III, Hpa I, Pst I, Pvu II, Sac I, Sca I, Stu I and Xba I, to recognize 6 specific base pairs, restriction patterns of all the enzymes except for Bgl B and Sca I were identical between the two breeds. When digested with Bgl B and Sca I, seven out of ten Korean native pigs showed different band patterns from those of Landrace, but the other three showed the same patterns as those of Landrace. While five BamH I restriction fragments were reported in literature, the seven fragments were detected in this study. The restriction map of Korean native pig mitochondrial genome with 28 cleavage sites of 8 different restriction enzymes was constructed.

Diagnostic usefulness of a T cell-based assay for latent tuberculosis infection in kidney transplant candidates before transplantation

Kim, S.-H.,Lee, S.-O.,Park, I.-A.,Park, S.J.,Choi, S.-H.,Kim, Y.S.,Woo, J.H.,Park, S.-K.,Park, J.S.,Kim, S.C.,Han, D.J. Blackwell Publishing Inc 2010 Transplant infectious disease Vol.12 No.2

<P>S.-H. Kim, S.-O. Lee, I.-A. Park, S.J. Park, S.-H. Choi, Y.S. Kim, J.H. Woo, S.-K. Park, J.S. Park, S.C. Kim, D.J. Han. Diagnostic usefulness of a T cell-based assay for latent tuberculosis infection in kidney transplant candidates before transplantation.Transpl Infect Dis 2010: <B>12:</B> 113–119. All rights reserved</P><P>Background</P><P>The presence of latent tuberculosis (TB) infection (LTBI) should be evaluated before kidney transplantation. Although a new T cell-based assay for diagnosing LTBI gave promising results, this assay has not yet been compared with the tuberculin skin test (TST) for diagnosing LTBI in renal transplant candidates before transplantation.</P><P>Patients and methods</P><P>All adult patients admitted to a single institute for renal transplantation over a 1-year period were prospectively enrolled. A clinically predictive risk of LTBI was defined as: (i) recent close contact with a person with pulmonary TB; (ii) abnormal chest radiography; (iii) a history of untreated or inadequately treated TB; or (iv) a new infection (i.e., a recent conversion of TST).</P><P>Results</P><P>Of 209 renal recipients, 47 (22%) had a positive TST≥5 mm, 21 (10%) had a positive TST≥10 mm, 65 (30%) had a positive T-SPOT.<I>TB</I> test, and 25 (12%) had an indeterminate T-SPOT.<I>TB</I> test. The induration size of TST was significantly associated with a high positivity rate on T-SPOT.<I>TB</I> (<I>P</I><0.001). Agreement between T-SPOT.<I>TB</I> test and TST≥10 mm was fair (<I>k</I>=0.24, 95% confidence interval 0.11–0.36). However, neither univariate nor multivariate analysis showed any association between the clinical risk for LTBI and positivity on T-SPOT.<I>TB</I> or TST.</P><P>Conclusion</P><P>T-SPOT.<I>TB</I> test was more frequently positive than TST in renal transplant candidates. However, further longitudinal studies are awaited to determine whether the ability of T-SPOT.<I>TB</I> assay to detect LTBI in renal transplant recipients can better predict the development of TB than can TST after transplantation.</P>

Electric Double Layer at the Interface of Ionic Liquid–Dielectric Liquid under Electric Field

Lee, D. W.,Im, D. J.,Kang, I. S. American Chemical Society 2013 Langmuir Vol.29 No.6

<P>The structure of the electric double layer (EDL) is analyzed in order to understand the electromechanical behavior of the interface of ionic liquid-dielectric liquid. The modified Poisson–Boltzmann equation proposed by Bazant et al. is solved to see the crowding and the overscreening effects that are the characteristics of an ionic liquid (Bazant, M. Z.; Storey, B. D.; Kornyshev, A. A. Double layer in ionic liquids: Overscreening versus crowding. <I>Phys. Rev. Lett.</I><B>2011</B>, <I>106</I>, 046102.). From the simple one-dimensional (1-D) analysis, it is found that the changes of the composition and the material properties in the EDL are negligible except under some extreme conditions such as strong electric field over<I>O</I>(10<SUP>8</SUP>) V/m. From the electromechanical view points, an ionic liquid behaves like a pure conductor at the interface with a dielectric liquid. Based on these findings, three specific application problems are considered. In the first, a new method is suggested for measuring the interfacial tension of an ionic liquid-dielectric liquid system. The deformation of a charged ionic liquid droplet translating between two electrodes is used for this measurement. The second is for the Taylor cone problem, which includes an extreme electric field condition near the tip. The size of the critical region, where the EDL effect should be considered, is estimated by using the 1-D analysis result. Numerical computation is also performed to see the profiles of electric potential and the electric stress along the interface of the Taylor cone. Lastly, the electrowetting problem of the ionic liquid is considered. The discrepancies in the results of previous workers are interpreted by using the results of the present work. It is shown that all the results might be consistent if the leaking of the dielectric layer and/or the adsorption of ions is considered.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/langd5/2013/langd5.2013.29.issue-6/la3040775/production/images/medium/la-2012-040775_0013.gif'></P>

d-pinitol regulates Th1/Th2 balance via suppressing Th2 immune response in ovalbumin-induced asthma

Lee, J.S.,Lee, C.M.,Jeong, Y.I.,Jung, I.D.,Kim, B.H.,Seong, E.Y.,Kim, J.I.,Choi, I.W.,Chung, H.Y.,Park, Y.M. North-Holland Pub ; Elsevier Science Ltd 2007 FEBS letters Vol.581 No.1

d-pinitol has been demonstrated to exert insulin-like and anti-inflammatory activities. However, its anti-allergic effect in the Th1/Th2 immune response is poorly understood. Recently, it was shown that T-bet and GATA-3 are master Th1 and Th2 regulatory transcription factors. In this study, we have attempted to determine whether d-pinitol regulates Th1/Th2 cytokine production, T-bet and GATA-3 gene expression in OVA-induced asthma model mice. We also examined to ascertain whether d-pinitol could influence eosinophil peroxidase (EPO) activity. After being sensitized and challenged with ovalbumin (OVA) showed typical asthmatic reactions. These reactions included an increase in the number of eosinophils in bronchoalveolar lavage (BAL) fluid, an increase in inflammatory cell infiltration into the lung tissue around blood vessels and airways, airway luminal narrowing, and the development of airway hyper-responsiveness (AHR). The administration of d-pinitol before the last airway OVA challenge resulted in a significant inhibition of all asthmatic reactions. Accordingly, this study may provide evidence that d-pinitol plays a critical role in the amelioration of the pathogenetic process of asthma in mice. These findings provide new insight into the immunopharmacological role of d-pinitol in terms of its effects in a murine model of asthma, and also broaden current perspectives in our understanding of the immunopharmacological functions of d-pinitol.

Two NF-κB inhibitor-alpha (IκBα) genes from rock bream (Oplegnathus fasciatus): Molecular characterization, genomic organization and mRNA expression analysis after immune stimulation

Lee, Y.,Umasuthan, N.,Whang, I.,Revathy, K.S.,Lee, S.,De Zoysa, M.,Oh, C.,Kang, D.H.,Noh, J.K.,Lee, J. Academic Press 2014 FISH AND SHELLFISH IMMUNOLOGY Vol.41 No.2

IκBα is a member of IκB family, which sequesters NF-κB in an inactivate form in the cytoplasm and blocks the translocation of NF-κB to nucleus. The IκBα paralogs of rock bream (OfIκBα-A and OfIκBα-B) encoded IκBα proteins with typical features including, highly conserved IκB degradation motif, six ankyrin repeats and a PEST sequence. However, their amino acid identity and similarity were only 55.6 and 69.7%, respectively suggesting that these two genes could be the two different isoforms of IκBα. The number and size of the exons of OfIκBα-A and OfIκBα-B were conserved well with all the compared vertebrate species, although they have significantly different genomic sizes. Phylogenetic analysis revealed that OfIκBα-A and OfIκBα-B proteins cluster with IκBα family members; however, they were grouped with different subclades in IκBα family. Tissue specific expression of OfIκBα mRNA was constitutively detected in all the tested tissues, and they showed the higher transcription level in heart, liver, gill and peripheral blood cells, respectively. The injection of flagellin stimulated the mRNA expression of OfIκBα paralogs in head kidney and intestine. Moreover, the OfIκBα mRNA expression in gill and liver was significantly up-regulated by LPS, poly I:C and Edwardsiella tarda challenges. The transcription of OfIκBα was up-regulated in early-phase of injection and then rapidly restored. These results suggest that the OfIκBα paralogs might be involved in rapid immune responsive reactions in rock bream against bacterial and viral pathogens.

Power-efficient and high-performance block I/O framework for mobile virtualization systems

Lee, K.,Lee, D.,Lee, S.,Eom, Y. I. Kluwer Academic Publishers 2017 The Journal of supercomputing Vol.73 No.4

<P>A virtualized system generally suffers from low I/O performance, mainly caused by its inherent abstraction overhead and frequent CPU transitions between the guest and hypervisor modes. The recent research of polling-based I/O virtualization partly solved the problem, but excessive polling trades intensive CPU usage for higher performance. This article presents a power-efficient and high-performance block I/O framework for a virtual machine, which allows us to use it even with a limited number of CPU cores in mobile or embedded systems. Our framework monitors system status, and dynamically switches the I/O process mode between the exit and polling modes, depending on the amounts of current I/O requests and CPU utilization. It also dynamically controls the polling interval to reduce redundant polling. The highly dynamic nature of our framework leads to improvements in I/O performance with lower CPU usage as well. Our experiments showed that our framework outperformed the existing exit-based mechanisms by 10.8 % higher I/O throughput, maintaining similar CPU usage by only 3.1 % increment. In comparison to the systems solely based on the polling mechanism, ours reduced the CPU usage roughly down to 10.0 % with no or negligible performance loss.</P>

Thermodynamic behaviors of excitonic emission in ZnO nanorods grown by pulsed laser deposition

Lee, Y.,Lee, D.J.,Cho, H.D.,Yoon, I.T.,Shon, Y.,Lee, S. North-Holland 2017 Journal of luminescence Vol.190 No.-

We investigated the thermodynamic behaviors of the exciton emission in ZnO nanorods that had been grown by laser ablation. The ZnO nanorods exhibited a clear luminescence peak from the neutral donor-bound exciton (D<SUP>0</SUP>X), which persisted near room temperature. Through analyzing temperature-dependences of photoluminescence properties, we found out insignificant thermal-quenching of D<SUP>0</SUP>X, arising from the large donor binding energy (i.e., E<SUB>bD(NR)</SUB> ~ 51.1 +/- 7.3meV). A small discrepancy of E<SUB>bD(NR)</SUB> from ZnO bulks' values (i.e., E<SUB>bD(Bulk)</SUB> = 53 - 72meV) is associated with inhomogeneous thermal-broadening factors such as defect-scattering at the surface of the nanorod. Despite of inhomogeneous thermal-broadening, the ZnO nanorods still have a high luminescence efficiency because of the weak homogeneous thermal-broadening effect (i.e., low exciton-phonon coupling).

RIHSA와 131I-Hippuran으로 측정한 심박출량의 비교

김정일,고창순,이안기,길광수,박진영,김동섭 대한핵의학회 1970 핵의학 분자영상 Vol.4 No.2

저자들은 정상성인 남자 11예에서 방사성동위원소인 RIHSA와 (131)^I-Hippuran을 사용하여 체외계측법에 의한 심박출량을 측정하여 다음과 같은 결론을 얻었다. 1) RIHSA를 사용한 심박출량의 측정치는 6750±866ml/min, 심계수의 측정치는 3960±476ml/min./㎡이었다. 2) 131I-Hippuran을 사용한 심박출량의 측정치는 5940±764ml/min, 심계수의 측정치는 3490±396ml/min./㎡이었다. 3) (131)^I-Hippuran에 의한 심박출량의 측정치는 RIHSA를 사용한 심박출량의 측정치의 88%로 나타났으며 양자간의 차이는 통계학적의미를 가지고 있었다(P$lt;0.05). 4) (131)^I-Hippuran을 사용하여 심박출량측정이 가능함을 알 수 있었다. Repeated measurement of cardiac output in the same 11 normal individuals were done with (131)^I-Hippuran and RIHSA. Following results were obtained. 1) The cardiac output measured with RIHSA was 6750±866ml/min. 2) The cardiac output measured with (131)^I-Hippuran was 5940±764ml/min. 3) The relative value of cardiac output measured with (131)^I-Hippuran to that measured with RIHSA was 88 percent, and a statistical significance was found present in the difference.

자외선 조사간격이 브로일러 병아리 다리 피부의 비타민 D3 함량에 미치는 영향

장윤환(Y . H . Chiang),조인호(I . H . Cho),여영수(Y . S . Yeoh),이은택(E .T . Lee),배은경(E . K . Bae),김중달(J . D . Kim),M . F . Holick(M . F . Holicka) 한국영양사료학회 1993 韓國營養飼料學會誌 Vol.17 No.6

Identification of thioredoxin domain-containing protein 17 from big-belly seahorse <i>Hippocampus abdominalis</i>: Molecular insights, immune responses, and functional characterization

Liyanage, D.S.,Omeka, W.K.M.,Yang, Hyerim,Godahewa, G.I.,Kwon, Hyukjae,Nam, Bo-Hye,Lee, Jehee Elsevier 2019 Fish & shellfish immunology Vol.86 No.-

<P><B>Abstract</B></P> <P>Thioredoxin domain-containing protein 17 (TXNDC17) is a small protein (∼14 kDa) involved in maintaining cellular redox homeostasis via a thiol-disulfide reductase activity. In this study, TXNDC17 was identified and characterized from <I>Hippocampus abdominalis</I>. The open reading frame (ORF) consisted of 369 bp and 123 amino acids. Similar to the other thioredoxins, TXNDC17 contained a conserved WCXXC functional motif. The highest spatial mRNA expressions of <I>HaTXNDC17</I> were observed in the muscle, brain, and intestine. Interestingly, the mRNA expression of <I>HaTXNDC17</I> in blood showed significant upregulation at 48 h against all the pathogen associated molecular patterns (PAMPs) and bacteria. Further, <I>HaTXNDC17</I> transcripts in the trunk kidney were significantly upregulated at 24–48 h by bacterial endotoxin lipopolysaccharides (LPS), viral mimic polyinosinic: polycytidylic acid (poly I:C), and gram-negative bacteria (<I>Edwardsiella tarda</I>). The DPPH assay showed that the radical scavenging activity varies in a concentration-dependent manner. The insulin reduction assay demonstrated a significant logarithmic relationship with the concentration of rHaTXNDC17. Moreover, FHM cells treated with recombinant HaTXNDC17 significantly enhanced cellular viability under oxidative stress. Together, these results show that HaTXNDC17 function is important for maintaining cellular redox homeostasis and that it is also involved in the immune mechanism in seahorses.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Big-belly seahorse thioredoxin domain containing protein 17 maintain redox homeostasis. </LI> <LI> TXNDC17 is ubiquitously found in cytosol and extracellular space. </LI> <LI> Thiol active CXXC conserved motif consists in HaTXNDC17. </LI> <LI> Spatial and temporal mRNA expression was evaluated. </LI> <LI> Radical scavenging ability, antioxidant activity and cellular viability were measured. </LI> </UL> </P>