Lactobacillus (LA-1) and butyrate inhibits osteoarthrits through controling autophagy and inflammatory cell death in chondrocyte

Keun hyung Cho,Hyun-Sik Na,JooYeon Jhun,Jiyoung Kim,Seung Yoon Lee,Jeong soo Lee,In Gyu Um,Seok Jung Kim,Mi-La Cho 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Osteoarthritis (OA) is a disease that reduces quality of life due to pain caused by persistent joint destruction. In addition, as a representative chronic disease, it causes inflammation and affects immunity, and it is one of the diseases that is difficult to cure, so treatment and improvement methods are urgently needed. In a previous study, we published that LA-1 improves osteoarthritis and has cartilage protection by controlling inflammation. However, it was not known how LA-1 improves osteoarthritis in the body. So in this study, it was confirmed that the administration of LA-1 to the MIA-induced OA rat model reduces the pain threshold, protects cartilage, and regulates inflammation markers in the articular synovium. Additionally, collecting and analyzing the feces of the disease model, it affected the gastrointestinal system and improved the environment of the microbiome. Interestingly, by providing LA-1, it was confirmed that the diversity and abundance of microbiome in the intestine were changed, and that the bacteria that produced SCFAs increased. In addition, daily supply of butyrate, one of the SCFAs produced by certain bacteria, triggers autophagy activation and tends to decrease necroptosis. This suggests that systemic immunity as well as OA is regulated according to changes in the intestinal microbial community, and that activation of autophagy can indirectly reduce abnormal cell death. In addition, assuming that osteoarthritis is a chronic degenerative disease, cell analysis was performed using splenocyte and blood assuming that the immune system is deteriorated. As a result, both splenocytes and PBMCs confirmed that regulatory T cells increased and Th17 cells decreased. In summary, providing LA-1 leads to increased production of SCFAs by altering the microbes in the intestine. Accordingly, it is possible to suppress the progression of OA and control pain due to OA, and improve an abnormal joint environment by controlling autophagy and necroptosis.

복합시술 모발의 자외선 보호처리에 따른 손상도와 색상 변화

라채숙ㆍ권수애ㆍ이옥규(hae Suk LaㆍSoo Ae KweonㆍOk Kyu Lee) 한국인체미용예술학회 2016 한국인체미용예술학회지 Vol.17 No.1

This study aimed to determine the effects of UV-blocking by the type of UV care agent for complex-treated hair. For this, healthy women’s hair was permed, bleached and dyed, and a Polypeptide Protein Treatment and hair sunscreen agent were applied. After that, changes in the degree of wave formation, hair surface conditions, physical properties of the hair and hair hue were examined by irradiating UVB rays. The analysis results were found as follows: In terms of the degree of wave formation, no difference was observed among UV care agents. In terms of hair surface scale, hair damage was lower when the hair was coated twice as opposed to being coated once regardless of the type of the UV care agent and treatment method. In addition, tensile strength, elongation rate and resilience rate were superior in the hair recoated with the hair sunscreen agent. In contrast, no significant difference was observed among the UV care agents in terms of damage rates. Lightness and reflected light were also high in the hair recoated with the hair sunscreen agent. In addition, the a* value, reflection and dominant wavelength were the highest in the hair with post-treatment coating for the skin while b* value, reflection and degree of irritation was the highest in the hair with post-treatment coating for hair. The said results can be summarized as follows: The UV care agent revealed effective UV-blocking effects by maintaining the wave formation and preventing hair damage. When UV rays were irradiated, the hair for post-treatment product was the most efficient in maintaining hair color.

소세포 폐암의 갑상선전이 1예

장리라,최영식,박요한,문대성,김자경,김성은,최인수,유찬희,남성진 고신대학교 의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.3

Metastasis to the thyroid gland is usually considered uncommon. The most frequent sites of the primary tumor include the kidney, breast and lung. Its presence often indicates poor prognosis. Metastases represent the advanced stage of the tumors and fine needle aspiration cytology is and important way of diagnosis in thyroid metastasis. we experienced a case of Metastatic Small cell Lung Carcinoma to the Thyroid Gland. A 58-year-old man with Graves' disease diagnosed pulmonary nodule in the left upper lung field on his chest X-ray film. The patient's thyroid gland was diffusely swollen, and elastic to hard. A thyroid ultrasonography showed 1cm sized hypodense nodule on the left thyroid gland. The patient underwent a ultrasonography guided fine needle aspiration cytology of the mass. Cytological diagnosis was a metastatic small cell carcinoma. Then Chemotherapy was administered to the patient. The authors report this rare case of metastatic small cell lung carcinoma to the thyroid with literature review.

초음파 근관기구를 이용한 근관충전법의 근관페쇄능에 관한 주사 전자현미경적 연구

최라영,이인숙 大韓齒科保存學會 1990 Restorative Dentistry & Endodontics Vol.15 No.2

AbstractThe purpose of this study was to evaluate the adaptation of root canal filling material to the dentinal wall of root canal and to compare the sealing ability of the root canal filling materials using ultrasonic endodontic instument with injection-molded thennoplasticized gutta-percha filling method and lateral condensation method.Fifty fresh human single root exiracted for orthodontic treatment, were randomly selected, and instrume- nted by step-back technique.And then, the teeth were divided into 5 groups according to each root canal filling methods. In the experimental group 1 and group 2, the root canals were filled with gutta perdia cases using ultrasonic instrument with and without sealer.In the experimental group 3 and 4, using jection - moldeed thennoplasticized gutta - percha method by obtui?canals were filled with and without sealer. In the control group, the canals were filled with sealer by lateral candensation. And then, 5 teeth of each group were immersed in black Indian ink, decalcified and cleared. The depth of dye penetration into the root canal were evaluated with stereoscope (Reichert Ltd., USA). Among the 5 teeth remaining in each group, the single longituding grooves were made on the labial and lingual root surfaces and then immersed in the liquid nitrogen to fracture the teeth spontaneously without any distortions of gutta-percha.Each specimens were examined with X-650 Scanning Electron Microscope(Hitachi ltd, Japan) to show the adaptation to the canal wall, void, homogenicity of filling material and location of .gutta-percha or sealer in the dentinal tubules of the root canal.The observations were as follows: 1. The experimental group 1 showed smaller mean dye penetration than control group, and showed the penetraton of sealer in the dentinal tubules of apical third of the root canal. 2. The experimental group 2 and group 4 showed the penetration of gutta - percha in the dentinal tubules of root canals. 3. The experimental group 1 and group 3 showed less mean dye penetration than the experimental group 2 and group 4. 4. The experimental group 1 and group 2 showed better adaptation of filling materials than control group.

위 MALT 림프종에서 다발성 림프절 전이로 오인된 동시성 림프절 결핵 1예

김성은,김규종,김도현,송준영,문대성,장리라,유찬희,문원,박무인,박선자,김영옥 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

28세 남자가 내시경적 점막절제술을 통해 저등급 위MALT 림프종으로 진단되었다. 병기설정을 위해 18F-FDG PET 검사를 시행하였고 우측 쇄골상부, 우측상/하부 기관주위, AP window 부위에 강한 섭취가 관찰되어 전이성 림프절로 판단하였다. 그러나, 우측 쇄골상부 림프절에서 시행한 절제생검에서 만성육아종성염증소견이 관찰되었고 조직의 결핵 중합효소연쇄반응에서도 양성 반응을 보였다. 2개월간 항결핵제의 사용 후 시행한 추적 컴퓨터단층촬영 검사에서 림프절 크기의 감소를 보여 최종적으로 위 MALT 림프종에서 광범위 림프절 전이로 오인된 동시성 림프절 결핵으로 진단되었다. A 28-year-old man was diagnosed as gastric MALT lymphoma in diagnostic EMR (endoscopic mucosal resection). He subsequently underwent an 18F-FDG PET. 18F-FDG PET showed intense multifocal uptake in right supraclavicular, right upper and lower paratracheal, and AP window areas that was considered radiologically to represent widespread metastatic lymphadenopathy. Excisional biopsy of a supraclavicular lymph node, however, revealed chronic granulomatous lymphadenitis with a positive reaction of Tb-nested PCR. After antituberculosis treatment for 2 months, a follow-up CT scan showed complete resolution of the lesions. Intense 18F-FDG uptake could be due to an infectious process such as synchronous tuberculous lymphadenitis. Therefore, this could mimic a malignant condition such as lymphoma with extensive lymph node metastasis due to a false-positive finding, which may lead to a misdiagnosis.

In vitro antiproliferative characteristics of flavonoids and diazepam on SNU-C4 colorectal adenocarcinoma cells

Lee, Sang-Woo,Lee, Jae-Tae,Lee, Maan-Gee,Lee, Ho Won,Ahn, Sohn Joo,Lee, Yong Jin,Lee, You La,Yoo, Jeongsoo,Ahn, Byeong-Cheol,Ha, Jeoung-Hee Springer-Verlag 2009 Journal of Natural Medicines Vol.63 No.2

Monosodium urate crystal-induced triggering receptor expressed on myeloid cells 1 is associated with acute gouty inflammation

Lee, Jennifer,Lee, Seon-Young,Lee, Jaeseon,Lee, Juhyun,Baek, Seungye,Lee, Dong-Gun,Kim, Eun-Kyung,Lee, Sung Hak,Cho, Mi-La,Kwok, Seung-Ki,Ju, Ji Hyeon,Park, Sung-Hwan Oxford University Press 2016 Rheumatology Vol.55 No.1

<P>Objective. Triggering receptor expressed on myeloid cells 1 (TREM-1), which amplifies the inflammation elicited by the Toll-like receptor pathway, was originally implicated in sepsis and bacterial infection. However, it has been suggested that TREM-1 may also play an important role in non-infectious inflammation. The present study was conducted to investigate whether TREM-1 is involved in human acute gouty inflammation. Methods. A total of 37 gout patients were recruited between March 2011 and January 2014 from Seoul St Mary's Hospital. The expression of TREM-1 on mononuclear cells was assessed using FACS analysis, immunostaining and real-time RT-PCR. To block the TREM-1 signal, soluble TREM-1 (sTREM-1) or the synthetic blocking peptide LP17 was used. The concentration of sTREM-1 was assessed by ELISA. Results. FACS analysis and real-time RT-PCR demonstrated that TREM-1 expression was higher in the SF mononuclear cells of acute gouty arthritis patients than in peripheral blood mononuclear cells (PBMCs). Immunohistochemical staining of tophi tissues revealed TREM-1 expression, with confocal microscopy demonstrating TREM-1 expression on tophi tissue macrophages. We also demonstrated that MSU treatment induced TREM-1 expression on the PBMCs of acute gout patients in vitro. Although blockade of TREM-1 did not directly suppress MSU-induced IL-1 beta production of PBMCs in vitro, the concentration of soluble TREM-1 was higher in the SF of gout vs OA patients and was positively correlated with serum CRP. Conclusion. TREM-1 is induced by MSU and is associated with the inflammation of human acute gouty arthritis.</P>

Optimization of Dendritic Cell-Mediated Cytotoxic T-Cell Activation by Tracking of Dendritic Cell Migration Using Reporter Gene Imaging

Lee, Hongje,Lee, Ho Won,La Lee, You,Jeon, Yong Hyun,Jeong, Shin Young,Lee, Sang-Woo,Lee, Jaetae,Ahn, Byeong-Cheol Springer-Verlag 2018 Molecular imaging and biology Vol.20 No.3

Targeting of hepatocellular carcinoma with glypican‐3‐targeting peptide ligand

Lee, You La,Ahn, Byeong‐,Cheol,Lee, Yongjin,Lee, Sang‐,Woo,Cho, Je‐,Yoel,Lee, Jaetae John Wiley Sons, Ltd 2011 Journal of Peptide Science Vol.17 No.11

<P>Hepatocellular carcinoma is a common malignancy. The carcinoma cells express glypican‐3 (GPC‐3) on the cell membrane. GPC‐3 is also expressed in melanoma cells. Therefore, GPC‐3 might be a potential target for tumor imaging or therapy. Here, proteomic mass spectrometry was used to identify peptides that target GPC‐3‐expressing tumors. A mammalian expression vector expressing a FLAG‐GPC‐3 fusion protein was cloned for immunoprecipitation. With the use of liposomes, the vector was transfected into HepG2 (HepG2/FLAG‐GPC‐3) and HEK 293 cells, and the transfected cell lines were selected with geneticin. HepG2/FLAG‐GPC‐3 cells were used for immunoprecipitation of FLAG‐GPC‐3 fusion protein. Seven peptide candidates (L1–L7) were selected for GPC‐3‐targeting ligands by mass spectrometric analysis. The L5 peptide with 14 amino acids (Arg‐Leu‐Asn‐Val‐Gly‐Gly‐Thr‐Tyr‐Phe‐Leu‐Thr‐Thr‐Arg‐Gln) showed selective binding to the GPC‐3‐expressing tumor cells, as did a shortened L5 peptide (L5‐2) with seven amino acids (Tyr‐Phe‐Leu‐Thr‐Thr‐Arg‐Gln). These peptide ligands have potential as targeting moieties to GPC‐3‐expressing tumors for diagnostic and/or therapeutic purposes. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd.</P>

Coenzyme Q10 Inhibits Th17 and STAT3 Signaling Pathways to Ameliorate Colitis in Mice

Lee, Seon-Yeong,Lee, Seung Hoon,Yang, Eun-Ji,Kim, Jae-Kyung,Kim, Eun-Kyung,Jung, KyungAh,Jung, Hongsoon,Lee, Kyungjin,Lee, Han Hee,Lee, Bo-In,Park, Sung-Hwan,Shin, Dong Yun,Cho, Mi-La Mary Ann Liebert, Inc. Publishers 2017 Journal of Medicinal Food Vol. No.

<P>Coenzyme Q10 (CoQ10) is a powerful antioxidant substance synthesized in the body. The current study aimed to determine whether CoQ10 suppresses inflammation and inhibits p-STAT3 expression in an experimental colitis mouse model. The mice were orally fed with CoQ10 once a day for 13 days. Histological analysis of the colons was performed by immunohistochemistry. Expression of IL-17, FOXP3, p53, AMPK, and mTOR and activation of p-STAT3 and p-STAT5 in lymph node and spleen tissues were detected by confocal microscopy of stained tissue sections. The relative mRNA expression was measured with real-time PCR, and protein levels were examined by western blot. CoQ10 reduced the disease activity index score and the colon histological score. It also reduced inflammatory mediators in the colon and increased the colon length. The expression of IL-17 and p-STAT3 was decreased with CoQ10 treatment. In contrast, CoQ10 treatment increased the expression of p-AMPK and FOXP3. Expression of anti-inflammatory cytokines was shown to increase in colitis mice treated with CoQ10. These results suggested that CoQ10 may reduce the severity of colitis and suppress inflammation through the inhibition of p-STAT3 and IL-17. These results support the use of CoQ10 as a potential targeted therapy for the treatment of colitis.</P>