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마우스 대장암 모델 구축 및 항암제 활성 평가를 위한 예비 연구
김예솔,강봉석,이상은,이은주,이경록,정상헌,박정숙 충남대학교 약학대학 의약품개발연구소 2014 藥學論文集 Vol.29 No.-
Abstract – Aberrant crypt foci (ACF) are early imorphological changes observed in rodents after administration of colon-specific carcinogen such as azoxymethane (AOM). ACF are considered to be putative preneoplastic lesions and are widely used as a surrogate biomarker to rapidly evaluate chemopreventive potential of compounds. The size of colorectal cancer was evaluated after administration of three anticancer drugs, 1 parent drug and 2 prodrugs. The body weights of mice were measured daily and considered as a surrogate for evaluation of general wellbeing. Colons were removed, cut along the longitudinal axis and flushed with phosphate-buffered saline. Each colon was cut into three equal lengths and fixed flat between filter papers. The fixed colon sections were stained with methylene blue. The number of ACF per colon, the number of aberrant crypts observed in each focus and the location of each focus were recorded. After single administration of AOM and multiple doses of anticancer drugs, no significant changes in the body weights of the mice was observed which was recorded for 52 days. However, an expected ACF was not observed in any treated groups. These findings suggest the induction of ACF in mice requires the promotion by dextran sulfate sodium as well as the initiation by AOM.
사무직 근로자의 수면의 질 및 직무스트레스와 우울과의 관련성
강미나,강수영,권수정,김현주,배재원,이보연,이예진,임의롬,정다영,조한솔 이화여자대학교 간호과학대학 2013 이화간호학회지 Vol.- No.47
Purpose: The purpose of this study was to understand the degree of depression, sleep quality, job stress and their association among office workers. Method: A self-administered questionnaire was distributed to 147 workers employed in 5 big enterprises, and 5 small and medium enterprises from October 21 to November 21, 2012. The questionnaires included socio-demographic and health-related characteristics, job-related characteristics, sleep quality, job stress (KOSS) and depression (CES-D). Result: Among all subjects, 23.1% was in the depression group (21 points and over in the CES-D score). Depressive level was positively correlated with job stress and quality of sleep. The adjusted odd ratio for the effects of sleep quality and job stress on depression significantly increased in the depression group compared to that of the normal group. Multiple regression analysis revealed that the following factors of influence had combined explanatory powers on depression: BMI, life satisfaction, quality of sleep, and job stress. Conclusion: The study revealed that complicated influences were exerted on the level of depression by variable factors, as well as socio-demographic characteristics, health-related characteristics, quality of sleep, and job stress. Specifically, the level of depression was influenced by the quality of sleep and job stress.
Kim, Sang Hoon,Kim, Ye-Sol,Jo, Yeong Deuk,Kang, Si-Yong,Ahn, Joon-Woo,Kang, Byoung-Cheorl,Kim, Jin-Baek International Society for Horticultural Science 2019 Scientia horticulturae Vol. No.
<P><B>Abstract</B></P> <P>γ-rays have been used to develop many mutations in diverse ornamental plants, including chrysanthemum, of which most changes have been flower color. Flower color is determined by plant pigments, including anthocyanins, which are affected by mutation of genes in the anthocyanin biosynthesis pathway. Sucrose and plant hormones also crosstalk in complex signaling pathways and modulate anthocyanin biosynthesis. In this study, we determined the optimal sucrose and plant hormone treatments for expression of anthocyanin biosynthesis genes and compared the mutation frequency among sucrose, methyl jasmonate (MeJA), and/or γ-ray treatments in chrysanthemum. Anthocyanin biosynthesis genes were highly expressed with 50 mM sucrose treatment for 18 h. The highest expression levels of anthocyanin biosynthesis genes were observed under sucrose with MeJA treatment followed by MeJA treatment alone. Sucrose with abscisic acid (ABA) treatment, and ABA or GA<SUB>3</SUB> treatment alone, had no effect on expression of anthocyanin biosynthesis genes. Sucrose with MeJA pre-treatment followed by γ-irradiation resulted in the highest mutation frequency (14.5%) among all treatments and a 1.5-fold increase in mutation frequency compared with γ-ray treatment alone. The ratio of anthocyanin-related flower-color mutants with sucrose and/or MeJA pre-treatments followed by γ-irradiation was higher than that with γ-ray treatment alone. Sucrose and/or MeJA modulated the expression of anthocyanin biosynthesis genes and highly expressed genes may be affected more readily by γ-rays, thus resulting in an increased frequency of anthocyanin-related flower-color mutants in chrysanthemum.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Sucrose and plant hormones modulate the expression of genes in the anthocyanin pathway. </LI> <LI> The expression of the genes was maximized at 18 h after treatment of 50 mM sucrose in chrysanthemum. </LI> <LI> The expression of the genes was highly increased by treatment of sucrose with methyl jasmonate. </LI> <LI> Pre-treatment of sucrose with methyl jasmonate increased the frequency of flower-color mutants induced by γ-irradiation. </LI> </UL> </P>
Kim Sol,Woo A La,Yong Seung Hyun,Leem Ah Young,Lee Su Hwan,Lee Sang Hoon,Kim Song Yee,Chung Kyungsoo,Kim Eun Young,Jung Ji Ye,Kang Young Ae,Park Moo Suk,Kim Young Sam,Park Youngmok 대한의학회 2024 Journal of Korean medical science Vol.39 No.20
Background: Coinfections with multiple nontuberculous mycobacterial (NTM) species have not been widely studied. We aimed to evaluate the clinical characteristics and treatment outcomes in patients with NTM-pulmonary disease (PD) caused by coinfection with multiple NTM species. Methods: We retrospectively reviewed patients with NTM-PD at a tertiary referral hospital in Korea between March 2012 and December 2018. Coinfection was defined as two or more species of NTM pathogens isolated from the same respiratory specimen or different specimens within three months. Results: Among 1,009 patients with NTM-PD, 147 (14.6%) NTM coinfections were observed (average age 64.7 years, 69.4% women). NTM species were identified more frequently (median 6 vs. 3 times, P < 0.001) in the coinfection group than in the single species group, and follow-up duration was also longer in the coinfection group (median 44.9 vs. 27.1 months, P < 0.001). Mycobacterium avium complex (MAC) and M. abscessus and M. massiliense (MAB) were the dominant combinations (n = 71, 48.3%). For patients treated for over six months in the MAC plus MAB group (n = 31), sputum culture conversion and microbiological cure were achieved in 67.7% and 41.9% of patients, respectively. We divided the MAC plus MAB coinfection group into three subgroups according to the target mycobacteria; however, no statistical differences were found in the treatment outcomes. Conclusion: In NTM-PD cases, a significant number of multiple NTM species coinfections occurred. Proper identification of all cultured NTM species through follow-up is necessary to detect multispecies coinfections. Further research is needed to understand the nature of NTM-PD in such cases.
Kim, Jung-Hee,Kim, Hee-Jong,Bak, Ye-Sol,Kang, Jeong-Woo,Lee, Dong-Hun,Kim, Man-Sub,Park, Yun-Sun,Kim, Eun-Jin,Jung, Kang-Yeoun,Lim, Yoong-Ho,Hong, Jin-Tae,Yoon, Do-Young The Korean Society for Applied Biological Chemisty 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.1
Anti-cancer effects of naringenin derivative diethyl (5,4'-dihydroxy flavanone-7-yl) phosphate were evaluated in human lung cancer cells. The effect of diethyl (5,4'-dihydroxy flavanone-7-yl) phosphate (dEdHF-7-p) on A549 cell viability was measured using MTS assay and cell counting. Morphological changes were detected using phase-contrast microscopy. Apoptosis was analyzed using Hoechst staining. The influence of dEdHF-7-p on cell cycle distribution was determined using propidium iodide (PI) staining, and protein expression was determined by Western blot analysis. A newly synthesized naringenin derivative dEdHF-7-p suppressed cell growth of A549 though mechanisms including inhibition of cell cycle and increased apoptosis. Apoptotic and cell cycle modulators were changed by dEdHF-7-p in A549 cells; cyclins, ppRB, and anti-apoptotic factor Bcl-2 were down-regulated, whereas apoptotic factor Bax and cyclin-dependent kinase inhibitors p21 and p53 were enhanced, thereby releasing cytochrome c into the cytosol of dEdHF-7-p-treated-A549 cells. dEdHF-7-p treatment processed caspases-3/-8/-9 and cleavage of poly ADP-ribose polymerase. The dEdHF-7-p treatment enhanced Fas expression and decreased expression of cell survival factors such as PI3K and p-Akt in a dose-dependent manner. Taken together, dEdHF-7-p induces apoptosis by inhibiting the PI3K/Akt survival signaling pathway and modulating mitochondria-emanated intrinsic and Fas extrinsic pathways in A549 cells.
Isotherm, kinetic and thermodynamic studies on the adsorption of paclitaxel onto Sylopute
Kim, Ye-Sol,Kim, Jin-Hyun Elsevier 2019 The Journal of chemical thermodynamics Vol.130 No.-
<P><B>Abstract</B></P> <P>Batch experimental studies were carried out on the adsorption of paclitaxel onto Sylopute, with the data then being fit to the Langmuir, Freundlich, Temkin and Dubinin-Radushkevich isotherm models. A comparison of the results revealed that, among the four isotherm models considered, the Freundlich isotherm model could account for the adsorption isotherm data with the highest accuracy. From the analysis of adsorption isotherms, it was found that the adsorption capacity increased with an increasing temperature and the adsorption of paclitaxel onto Sylopute was favourable. The obtained kinetics data for paclitaxel adsorption with Sylopute agreed well with the pseudo-second-order model. In addition, according to the intraparticle diffusion model, intraparticle diffusion played a dominant role in paclitaxel adsorption. Thermodynamic parameters revealed the endothermic, irreversible, and nonspontaneous nature of the adsorption.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Freundlich isotherm accounted for adsorption data of paclitaxel on Sylopute. </LI> <LI> The adsorption process was physical in nature. </LI> <LI> The kinetics data agreed well with the pseudo-second-order model. </LI> <LI> The adsorption process was endothermic, irreversible and nonspontaneous. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>