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Estradiol-17β를 처리한 흰쥐 자궁에 있어서 Clomiphene이 세포내 수용체, DNA 및 cAMP 농도에 미치는 영향
김곤섭,고복현 동국대학교 경주대학 1989 論文集 Vol.8 No.-
These experiments were carried out to investigate the study on the interaction of estradiol receptor and cAMP level of clomiphene in immature rat uterus. The results obtained were summarized as follow : 1. The binding affinity of clomiphene to estradiol receptor in uterine cytosol was lower than that of estradiol-17β. clomiphene inhibited the replenishment of the receptor to cystol in uterus(P<0.01). 2. DNA level in uterus was increased at 48 hours after estradiol-17β or clomiphene injection(P<0.01) 3. cAMP level in uterus was increased at 48 hours after estradiol-17β or clomiphene injection(P<0.01)
A Study on Ocular and Skin Irritation of a New Disinfectant, Clean Natural<SUP>®</SUP>
Suk Kim,Myung Sun Chun,Hee Sik Chung,Won Chul Jung,Dong Hyeok Kim,Jeong Ju Lim,Ho Yeong Shon,Gon Sup Kim,Hu-Jang Lee 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.1
This test was performed to evaluate the ocular and skin irritation of Clean Natural<SUP>®</SUP>", a new disinfectant of which main components are propolis and wood vinegar from Quercus mongolica. The results of eye irritation test were as followings; in the single application test, 0.1 ㎖ Clean Natural<SUP>®</SUP>" eyedrops and 0.9% saline as vehicle control were applied to one eye of rabbit, respectively. The reactions were examined for 7 days after treatment. Transient, Up to 2 days, eye irritation was observed in the rabbit eyes treated with the test solution. The results of skin irritation test were as following; there were no observed clinical signs, body weight changes and gross pathologic findings during experimental period. There were no observed erythema, eschar, and edema formation on intact and abraded skin. The primary irritation index (P.LL) of Clean Natural<SUP>®</SUP>" was 0 and was evaluated none irritating product. In the present study, 10% Clean Natural<SUP>®</SUP>" was evaluated as minimally irritating and none-irritating product for eye and skin, respectively.
Munki Kim, Chong-Sup Kim, Gon-Sup Kim, Chung-Kil Won 충북대학교 동물의학연구소 2014 Journal of Biomedical and Translational Research Vol.15 No.3
The morphology of the lingual papillae in a female Bengal tiger (Panthera tigris tigris) was examined by scanning electron microscopy (SEM). The tongue was 22.3 cm in length and 7.1 cm in width. Numerous filiform papillae were distributed over the entire dorsal surface of the tongue. SEM examination of the tongue revealed two types of mechanical papillae, i.e. filiform and conical papilla, and two types of gustatory papillae, i.e. fungiform and vallate papilla, on the dorsal surface of the tongue. Each filiform papilla consisted of one primary papilla and several secondary papillae. The filiform papillae on the anterior part of the tongue were divided into one primary and 6~14 secondary papillae. Unlike other mammalians, however, secondary papillae in the mid-part of the tongue showed pineal-like papillae. In the posterior part of the tongue, secondary papillae were rare or absent. Fungiform papillae were surrounded by filiform papillae and densely distributed on the lingual surface. There were two vallate papillae on the borderline between the lingual body and root of the tongue. A vallate papilla contained two secondary papillae inside the grooves. Conical papillae were located in the area of the vallate papillae and covered the posterior part of the tongue root. No foliate papillae were seen on both margins of the posterior part of the tongue. Our results indicate that the structure on the lingual papillae of the Bengal tiger is somewhat different from that of other mammals.
Young-Suk Kim,Boh-Hyun Kim,Gon-Sup Kim,Joung-Soon Jang,So-Young Kim,Byeong-Dae Choi,Jeong-Ok Kim,Yeong-Lae Ha 충북대학교 동물의학연구소 2014 Journal of Biomedical and Translational Research Vol.15 No.4
Glycoproteins isolated from fruit bodies and mycelial cultures of mushrooms exhibit anti-carcinogenic actions in human cancer cells and animal tumor cells by induction of apoptosis. Here, we report that isoflavone-conjugated glycoproteins (designate Gluvone), exhibit strong anti-carcinogenic effects on human breast cancer MCF-7 cells by induction of apoptosis. Gluvone with 9.4 kDa of molecular weight was isolated from submerged-liquid culture of Agaricus blazei mycelia (ABM) in soy flake-containing liquid medium. MCF-7 cells were incubated with various amounts of Gluvone (0~250 μM) for a period of 6 days. Gluvone exhibited anti-proliferative actions in a dose-dependent manner and 62% growth inhibition at 200 μM for 4 days relative to control. Hoechst 33258 staining analysis revealed that Gluvone induced formation of apoptotic bodies. Gluvone was associated with down-regulation of anti-apoptotic Bcl-2 protein expression as well as up-regulation of pro-apoptotic Bax protein expression. Gluvone treatment induced proteolytic activation of caspase-9 and caspase-3 through cytochrome c release from mitochondria to cytosol as well as concomitant degradation of poly (ADP-ribose) polymerase (PARP). In addition, Gluvone induced activation of caspase-8. Taken all together, these results indicate that the anti-proliferative effect of Gluvone is associated with induction of apoptotic cell death through the mitochondrial dysfunction pathway mediated by enhancement of Bax protein expression and suppression of Bcl-2 protein expression.
Kim, Gon Sup,Kim, Dong Hyeok,Lim, Jeong Ju,Lee, Jin Ju,Han, Dae Yong,Lee, Whi Min,Jung, Won Chul,Min, Won Gi,Won, Chung Gil,Rhee, Man Hee,Lee, Hu Jang,Kim, Suk Pharmaceutical Society of Japan 2008 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.31 No.11
<P>Salmonellosis is a major bacterial zoonosis that causes a variety of disease syndromes, from self-limiting enteritis to fatal infection in animals and food-borne infection and typhoid fever in humans. Recently, the emergence of multidrug-resistant strains of <I>Salmonella</I> sp. has caused more serious problems in public health. The present study investigated the antibacterial effects of <I>Houttuynia cordata</I> water extract (HCWE) against murine salmonellosis. In RAW 264.7 cells, there was no detectable cytotoxic effect of HCWE at any concentration between 25 and 100 μg/ml after 8-h incubation. The antibacterial activity of HCWE was then examined in a <I>Salmonella enterica</I> serovar (<I>Salmonella typhimurium</I>), and was found to increase in a dose-dependent manner at concentrations from 25 to 100 μg/ml during 8-h incubation. HCWE also affected RAW 264.7 cells including morphologic change and bacterial uptake, but there was no significant difference in bacterial replication in RAW 264.7 cells. With HCWE alone, nitric oxide (NO) production by RAW 264.7 cells did not increase, but when RAW 264.7 cells were infected by <I>S. typhimurium</I>, with or without HCWE, NO production with HCWE was 2-fold higher than that without HCWE. Treatment with HCWE did not affect inducible NO synthase (iNOS) mRNA expression by RAW 264.7 cells, but when RAW 264.7 cells with HCWE were infected by <I>S. typhimurium</I>, iNOS mRNA expression was increased during 8-h incubation. Furthermore, HCWE showed virulence reduction effects in <I>S. typhimurium</I>-infected BALB/c mice. After a lethal dose of <I>S. typhimurium</I>, the mortality rate in the HCWE untreated group was 100% at 7 d, but the HCWE 25, 50, and 100 μg/ml groups survived until 11, 17, and 23 d, respectively. These data suggest that HCWE is stable and beneficial in the treatment of bacterial infection including intracellularly replicating pathogens and may solve antimicrobial misuse and overuse.</P>
Effects of Hyperbaric Oxygen and $\alpha$-Tocopherol on Skin Antioxidant Enzymes Defence in Rats
Kim, Jang-Shu,Kim, Chung-Hui,Kim, Gon-Sup,Hah, Dae-Sik,Park, Sun-Gun,Kim, Yang-Mi The Korean Society of Toxicology Korea Environment 2001 Toxicological Research Vol.17 No.1
In order to test the effects of hyperbaric oxygen (HBO) and $\alpha$-tocopherol on full-thickness skin grafts in rats, we peeformed full-thickness skin grafts bilaterally on rats. After surgery, we analyzed the tissue-concentrations of superoxide dismutase (SOD), catalase, and glutathione peroxidase(GPx)/reductase(GPr) on days 0, 2, 4, 7, 10, 14, 21 and 28. The four groups had similar patterns of change in SOD, catalase, GPx and GPr values. SOD increased initially, and was significantly increased at day 7, returning to the preoperative activity level on day 14 (control, HBO, and $\alpha$-tocopherol treated alone) and 28 (HBO plus $\alpha$-tocopherol). Catalase had a similar pattern of change as the SOD enzyme activity, except for the surgical control on day 2. Glutathione peroxidase/reductase activity in the four groups had a similar pat-tern of enzyme activity, with a significant increase from preoperative level on day 4, peaking during days 7 to 10, and returning to preoperative level on day 21(surgical control, HBO, and $\alpha$-tocopherol-treated alone) and 28 (HBO plus $\alpha$-tocopherol treated group). Hence, the clinical use of HBO and $\alpha$-tocopherol mixture can be recommended as an adjunctive treatment for free skin grafts in rats. But, the antioxidant used, its dose, and the timing of its administration, as well as, the exposure time and the pressure of HBO, should be the subject of further research.